Rezumatul tezei de doctorat - USAMV Cluj-Napoca

the minimum growing diameter, of 31,57 mm, was realized by the colonies inoculated from
Motiş water samples, and the maximum diameter, of 38,11 mm, the water samples from
Ariniş.
II.2.2. Comparative results regarding the development
of Saprolegnia colonies on the liquid culture media
The usage of liquid culture media is necessary to obtain bigger quantities of fungal
mass, for DNA extraction.
This experiment was performed to observe and recommend the best transfer
combination from solid culture medium to the liquid one, estimating the fungal mass
quantity, in cm 3 .
There were observed and analyzed 138 samples, 69 samples on each culture medium
combination. Daily observations made from the transfer day up to the seventh day indicated
almost a double rhythm of development in Potato Dextrose Broth (PDB) liquid medium
compared to the samples in Sabouraud Dextrose Broth (SDB) liquid medium. In day seven,
in all 100 ml Erlenmeyer glasses, the fungal pellets from Potato Dextrose Broth (PDB)
liquid medium had between 2,5 and 4 cm 3 , compared to the colonies developed in Potato
Dextrose Broth (PDB) liquid medium, with values of 1-2 cm 3 .
Further to the results, only the fungal samples obtained in bigger quantities on Potato
Dextrose Broth (PDB) liquid medium were analyzed.
II.3. THE MORPHOLOGICAL DESCRIPTION OF SAPROLEGNIA STRAINS
The microscope observations indicated the fact that the hyphae have a characteristic
unsegmented aspect of lower fungi. There were not analyzed the hyphal length and
thickness, only the morphological general aspect, which confirmed the presence of
saprolegnian fungi in the culture, without any morphological differentiation between genera
and species. The microscope observations performed on a period of 21 days evaluated at
every sample the presence or the absence of sexual reproduction organs, as well as the
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