Rezumatul tezei de doctorat - USAMV Cluj-Napoca

I.3.3.1. Solid culture media used in the experiment
The two solid culture media used in the experiment were the following (according to
http://www.sigmaaldrich.com):
Potato Dextrose Agar (PDA) (Fluka)
Sabouraud 2% Glucose Agar (SGA) (Fluka)
From each fishpond, we made an inoculation in the two solid culture media used
(PDA, SGA), from the most developed sample, for making comparative observations
respecting the developing mode of fungal species from Saprolegniaceae family.
For testing the growing rate on agar solid medium, in Petri dishes containing the two
solid culture media (PDA, SGA) and incubated at 22°C ± 2°C for 72 hours, we observed
Saprolegnia’s hyphae growing rate. The radial diameter growth was measured at every 24
hours. The hyphae reached the maximum capacity of radial growth, when those have get
until the edge of Petri dises (>40 mm) (adapted after Stueland and al., 2005).
At 72 hours of development on the solid media, fungal colonies were moved on the
liquid ones, in the following procedure: the colonies from Potato Dextrose Agar (PDA) solid
medium were transfered on Potato Dextrose Broth (PDB) liquid medium, and the colonies
from Sabouraud 2% Glucose Agar (SGA) solid medium, on Sabouraud Dextrose Broth
(SDB) liquid medium. This experiment was performed to be able to observe and recommend
the best transfer procedure from a solid medium to a liquid one.
I.3.3.2. Liquid culture media used in the experiment
The liquid media used in the experiment were the following (according to
http://www.sigmaaldrich.com):
Potato Dextrose Broth (PDB) (Fluka)
Sabouraud Dextrose Broth (SDB) (Fluka)
All the saprolegnian colonies from the solid media were moved in Erlenmeyer
glasses, with 100 ml antibiotic liquid medium and kept in an rotary shaker incubator, at the
temperature of 22°C ± 2°C, for 5-7 days, depending on the developing degree. The mycelial
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