Rezumatul tezei de doctorat - USAMV Cluj-Napoca

The experimental model used, included the collecting of 5 water samples from each
fishpond, from the 4 lake sides and from centre, and 50 cm medium depth. From the 5
samples we made a single water sample, which was subsequent analyzed in laboratory
conditions.
For the identification of different species from Saprolegniaceae family, we collected
and analyzed water samples, carp species and spawns affected by disease, from Transylvania
monitored fishponds. The water samples were collected in 2 litres recipients, from the 5
areas of each fishpond, then the 5 samples were mixed in a 15 litres tank, collecting for the
analyze a single blended sample from each fishpond, in a 2 litres recipient. The transport
was made in the first 12-24 hours from the collection and the analysis were performed in the
next day.
I.3.2. The initiation of Saprolegnia culture in laboratory conditions
To ensure that the possible differences, which could appear between the fungal
species from Saprolegniaceae family present in the water, could be strict attributed to the
differences given by water nature and the species which populates it, we performed as it
follows: from each water sample, in a quantity of 2 litres, we made up 3 samples of 100 ml,
in plastic sterile recipients. In each recipient there were introduced 10-15 spawns from the
same carp female. Because the literature offers contradictory data respecting Saprolegnia’s
development temperature in different world areals, we suggested the testing of the fungus
growing and development mode, at 3 levels of temperature: 10°C, 15°C and 22°C.
Water samples, containing 10-15 spawns for artificial infestation, were introduced in
a incubator, following the temperatures mentioned, between 3-7 days. In this period we
made daily observations on each sample, to find out the starting moment of fungus
infestation upon spawns and the intensity of its development at that temperature.
There was constituted a number of 207 samples for each collecting month, 3 samples
for each fishpond, which were monitored at the 3 temperature levels, and making an
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