SLOVENIAN VETERINARY RESEARCH
SLOVENIAN VETERINARY RESEARCH
SLOVENIAN VETERINARY RESEARCH
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Slov Vet Res 2012; 49 (1): 51- 8<br />
UDC 636.2.082.4:591.463.1<br />
Original Scientific Article<br />
Improvement of sperm sorting efficiency and<br />
fertilizing capacity employing two variations of a<br />
new bull semen extender (Sexcess ® )<br />
Primož Klinc 1 *, Detlef Rath 2<br />
1<br />
Clinic for Reproduction and Horses, Veterinary Faculty, Gerbičeva 60, 1000 Ljubljana, Slovenia; 2 Friedrich-Loeffler-Institut, Institute of Farm<br />
Animal Genetics Hoeltystr. 10, 31535 Neustadt, Germany<br />
*Corresponding author, E-mail: primoz.klinc@vf.uni-lj.si<br />
Summary: The aim of the present study was to analyse the effect of temporary chemical inhibition of sperm motility before and<br />
during flow cytometrical sorting on the quality and fertilizing capacity of sex sorted and frozen/thawed spermatozoa. The quality<br />
and fertilizing capacity of temporarily immobilized spermatozoa sorted in the presence of sodium fluoride (S-AO-NF) was compared<br />
with sorted semen using a standard protocol as developed in our laboratory (S-AO) and with unsorted control (C). Semen<br />
from two bulls (Holstein Friesian and Limousine) was used and in total 283 first inseminations with 2 million live, frozen/thawed sex<br />
sorted and unsorted spermatozoa were performed on 197 farms. Motility of spermatozoa was completely inhibited after addition<br />
of sodium fluoride and after sorting and centrifugation returned to a similar degree as before. Motility after thawing and incubation<br />
at 37°C did not differ between groups. After further incubation at 37°C for 12 h and 24 h motility was significantly higher (P