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SLOVENIAN VETERINARY RESEARCH

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22<br />

Ö. Pelin Can<br />

9<br />

8<br />

7<br />

a<br />

8<br />

7<br />

b<br />

6<br />

6<br />

5<br />

4<br />

5<br />

log cfu/g<br />

3<br />

2<br />

1<br />

log cfu/g<br />

4<br />

3<br />

0<br />

0 2 4 6 8 10 12 14<br />

2<br />

0 2 4 6 8 10 12 14<br />

Storage Time (Day)<br />

Storage Time (Day<br />

8<br />

c<br />

8<br />

d<br />

6<br />

6<br />

4<br />

4<br />

log cfu/g<br />

2<br />

log cfu/g<br />

2<br />

0<br />

0 2 4 6 8 10 12 14<br />

0<br />

0 2 4 6 8 10 12 14<br />

Storage Time (Day)<br />

Storage Time (Day)<br />

Figure 1: Changes (log cfu/g) in (a) Total Viable Counts (TVC); (b) Pseudomonas spp.; (c) LAB and (d) Enterobacteriaceae,<br />

control (A), batter with added thyme EO 0.4% (B) and ball surface b thyme EO 0.4% (C). Each point is the mean<br />

2,4<br />

of three samples taken from two replicate experiments (n: 3 x 2: 6). 2,1 Error bars show SD.<br />

1,8<br />

7,5<br />

7,2<br />

a<br />

1,5<br />

2,4<br />

b<br />

1,2<br />

2,1<br />

1,8 0,9<br />

6,9<br />

6,6<br />

6,3<br />

6,0<br />

0 2 4 6 8 10 12 14<br />

Storage Time (Day)<br />

mg malondialdehyde/kg mg malondialdehyde/kg<br />

1,5 0,6<br />

1,2 0,3<br />

0,9<br />

0,0<br />

0 2 4 6 8 10 12 14<br />

0,6<br />

Storage Time (Day)<br />

0,3<br />

0,0<br />

0 2 4 6 8 10 12 14<br />

Storage Time (Day)<br />

Figure 2: Changes of pH (a) and TBA (b) values of chicken balls, control (A), batter with added thyme EO 0.4% (B)<br />

and ball surface thyme EO 0.4% (C). Each point is the mean of three samples taken from two replicate experiments<br />

(n: 3 x 2: 6). Error bars show SD.<br />

The sensory properties (odour and taste) of the<br />

cooked chicken balls are given in Fig. 3a-b. Sensory<br />

analysis was carried out until the 6 th day of<br />

storage for control group samples and until the<br />

12 th day of storage for treatment group samples.<br />

Due to the microbiological quality of the control<br />

group samples, exceeded limit values were not<br />

subject to sensorial evaluation. Taste attribute<br />

has usually been a more sensitive parameter than<br />

odour; therefore, taste attribute was used for the<br />

sensory evaluation of the thyme products and the<br />

determination of sensorial shelf-life in the present<br />

study. On day 0 of storage, the cooked chicken<br />

balls had a pleasant taste and odour. The presence<br />

of thyme oil (on ball surface) in the samples<br />

produced a very pleasant taste (P

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