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Name: __________________________________________ Date: ___________ Period: _________<br />
Data Table 3<br />
Well Contents Initial pH Final pH Observations<br />
1 Oil + Water<br />
2 Oil + Water +<br />
Lipase<br />
3 Oil + Water +<br />
Lipase + Soap<br />
PART C Analysis Questions - on a separate sheet of paper complete the following<br />
1. How does the pH of each solution show that fat digestion has occurred?<br />
2. Which well showed the greatest degree of fat digestion? Explain.<br />
3. Where does fat digestion take place in the human digestive system?<br />
4. Describe the effects of bile on fat.<br />
5. Why is emulsification necessary for efficient digestion of fats, but not for proteins or<br />
carbohydrates?<br />
6. What effect would fat digestion have on the pH of the surrounding solution? Explain.<br />
7. Predict the effect of a blocked bile duct on the composition of a patient’s stool.<br />
Data Table 4<br />
Well Contents Initial pH Final pH Observations<br />
1 Food sample<br />
2 Food sample +<br />
Pepsin + HCl<br />
3 Food sample<br />
4 Food sample +<br />
Amylase<br />
5 Food sample +<br />
Lipase + Soap<br />
PART D Analysis Questions - on a separate sheet of paper complete the following<br />
1. Summarize the pH changes in each well.<br />
2. What macromolecules were present in your food sample? Was this what you expected?<br />
Explain your answer.<br />
3. What macromolecules do you think you might find in the following foods: bread, eggs,<br />
broccoli, apple, steak, potato chips.<br />
4. CONCLUSION: In 1-2 paragraphs summarize the procedure and results of this lab.<br />
Adapted from Neo/Sci Food <strong>Digestion</strong> Lab Activity 2011 240