17 Digestion - Student.pdf - haspi

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Name: __________________________________________ Date: ___________ Period: _________ The 3 Major Digestible Macromolecules Protein Carbohydrate Lipids Polypeptide Polysaccharide Fat Molecule Polymer http://users.rcn.com/jkimball.ma.ultra net/BiologyPages/P/Peptide.gif Amino Acid http://bioweb.wku.edu/courses/biol115/ wyatt/biochem/Carbos/Carb_poly.gif Monosaccharide http://www.foodmuseum.com/images /exfatMoleculeandCells.jpg Fatty Acid or Cholesterol Monomer Build and repair body tissues Glucose http://kentsimmons.uwinnipeg.ca/cm150 4/Image69.gif Energy http://www.protocolsupplements.com/Sports- Performance-Supplements/wpcontent/uploads/2009/06/amino-acid-mcat1.png http://www.clker.com/clipart- 10765.html Stored energy Use in the Body http://content.contentthatworks.com/image s_articles/2006/health/health_20060724_car egiver_flexedarm.jpg Meat, eggs, beans http://pullenl.wonecks.net/files/2010/09/ Children-Running-Outside.jpg Breads, pasta, fruits, vegetables http://photos.demandstudios.com/ge tty/article/103/163/82370075_XS.jpg Oils, butter, meat, dairy, fast food Foods Sources http://www.gethenchnow.com/wpcontent/uploads/2011/01/high_protein _foods.jpg http://www.magazine.ayurvediccure.com/wpcontent/uploads/2009/02/carbohydrate.jpg http://cdn2- b.examiner.com/sites/default/files/styles/i mage_full_width/hash/af/13/af1309885ddd ea6427ea13af260f4ca4.jpg NIH. 2008. Your Digestive System and How it Works. National Digestive Diseases Information Clearinghouse, NIH Publication No. 08-2681. www.digestive.niddk.nih.gov Adapted from Neo/Sci Food Digestion Lab Activity 2011 236
Name: __________________________________________ Date: ___________ Period: _________ Materials Spot plate 2% Albumin (protein) 1% Hydrochloric acid 20 pH strips 3% Pepsin Biuret 10 stirring sticks 1% Starch solution 2% Amylase Forceps Potassium iodine Corn oil Paper towels Water Liquid soap Procedure Purpose: The goal of this lab will be to observe how digestive enzymes are able to break down macromolecules. All data tables are located in the analysis portion of the lab. PART A: Protein Digestion In part A the albumin represents a polypeptide protein. Pepsin is the enzyme needed to break down the albumin into amino acids, but can only work within a certain pH range. The Biuret will turn pink with smaller protein chains and will turn purple in the presence of large proteins. 1. Using a pencil, label the wells on your spot plate 1-5. 2. Place 5 drops of 2% Albumin in wells 1-4. 3. Add 5 drops of 1% HCl to wellS 2, 4, and 5. 4. Add 5 drops of 3% Pepsin to wells 3 and 4. 5. Use separate stirring sticks to mix each well, and allow them to sit for 5-7 minutes. 6. Use the forceps to dip a separate pH strip into each of the wells 1-5. Compare the color change of each strip to the pH strip chart to determine the pH of the mixture in each well. Record your results in Data Table 1. 7. Add 2 drops of Biuret to wells 1-5. A positive test for protein breakdown will turn pink. If the well is positive, put a + in Data Table 1. If the well is negative put a -. 8. Record your observations of each of the mixtures in Data Table 1. 9. Rinse out your spot plate and dry it off with a paper towel. PART B: Carbohydrate Digestion In part B starch is a polysaccharide, and amylase is the enzyme responsible for breaking starch down into monosaccharides. Since the potassium iodine tests for starch, you will be looking for a negative test to determine whether amylase actually broke down the starch. 1. Using a pencil, label wells 1 and 2 on the spot plate. 2. Add 15 drops of 1% Starch solution to wells 1 and 2. 3. Add 5 drops of 2% Amylase solution to well 2. 4. Use separate stirring sticks to mix each well, and allow them to sit for 5-7 minutes. 5. Add 1 drop of Potassium iodine to wells 1 and 2. A positive test for starch will turn dark blueblack. If a well is positive for starch, put a + in the Data Table 2. If the test is negative for starch put a –. 6. Record your observations of each of the mixtures in Data Table 2. 7. Rinse out your spot plate and dry it off with a paper towel. Adapted from Neo/Sci Food Digestion Lab Activity 2011 237
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