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C y t ogenetics
morphology, and c e r t a i n a n a t o m i c a l f e a t u r e s ,
and by t h e success of the cross C. cajan
FC 9334 x A. scarabaeoidee. It is t h e r e -
f o r e proposed t h a t Atylosia be i n c o r p o r a t e d
i n t o t h e genus Cajanus, w i t h cytotaxonomic
r e v i s i o n o f t h e l a t t e r .
SHARMA, C.N. 1967.
S t u d i e s on t h e Cajanus and Atylosia h y b r i d .
D I I T (1967) T h e s i s . I n d i a n I n s t i t u t e o f
Technology, Kharagpur, West B e n g a l ,
I n d i a . 574
SHRIVASTAVA, M.P. 1975.
E f f e c t of gamma i r r a d i a t i o n on d i p l o i d and
t e t r a p l o i d seeds of Cajanus cajan ( L . )
M i l l s p . C u r r . S c i . 4 4 ( 5 ) : 1 6 7 - 1 6 8 . 575
Normal d i p l o i d and c o l c h i c i n e - i n d u c e d t e t r a ­
p l o i d seeds were t r e a t e d a t f i v e doses o f
- r a y s and s t u d i e d f o r g e r m i n a t i o n , p e r c e n t ­
age s u r v i v a l , s e e d l i n g h e i g h t , and number
o f l e a v e s . I n d i p l o i d s , i n c r e a s i n g doses,
15 to 60 K r a d , r e s u l t e d in a decrease f o r
a l l these c h a r a c t e r s . I n t h e t e t r a p l o i d s ,
1 5 Krad r e s u l t e d i n i n c r e a s e d g e r m i n a t i o n ,
h e i g h t , and number of leaves compared w i t h
u n t r e a t e d seeds. A t h i g h e r doses, g e r m i ­
n a t i o n remained s i m i l a r t o t h a t o f t h e
d i p l o i d s except a t t h e h i g h e s t dose o f
6 0 K r a d , when i t was l e s s . S u r v i v a l i n a l l
cases was h i g h e r than t h a t o f t h e d i p l o i d s ;
h e i g h t was g r e a t e r a t a l l doses except
60 K r a d , when it was t h e same as in t h e
d i p l o i d s ; and t h e number o f leaves i n a l l
cases was h i g h e r t h a n i n t h e d i p l o i d s .
SHRIVASTAVA, M.P., and R.K. JOSHI. 1972.
A smear t e c h n i q u e f o r r o o t t i p chromosome
p r e p a r a t i o n o f Cajanus c a j a n ( L . ) M i l l s p .
JNKW Res. J. 6 : 5 9 - 6 0 . 576
Of s i x chemicals t e s t e d , - bromonaphthalene
was most s u c c e s s f u l i n p r e t r e a t m e n t f o r
Feulgen s t a i n i n g . A t somatic metaphase,
2n - 22 was o b s e r v e d .
SHRIVASTAVA, M . P . , LAXMAN SINGH, and
R.K. JOSHI. 1972.
I n d u c t i o n and c y t o m o r p h o l o g i c a l study o f
a u t o t e t r a p l o i d y i n Cajanus c a j a n ( L . )
M i l l s p . JNKW Res. J . 6 : 4 7 - 5 0 . 577
A l l 1 3 t e t r a p l o i d s induced b y c o l c h i c i n e
t r e a t m e n t showed reduced seed s e t and
y i e l d . C y t o l o g i c a l s t u d i e s showed m e i o t i c
i r r e g u l a r i t i e s b u t p h y s i o l o g i c a l imbalances
were a l s o a p p a r e n t l y i n v o l v e d i n
c a u s i n g s t e r i l i t y .
SHRIVASTAVA, M . P . , D. SHARMA, and
LAXMAN SINGH. 1973.
Karyotype a n a l y s i s i n 1 5 v a r i e t i e s o f
Cajanus aajan ( L . ) M i l l s p . and Atylosia
lineata (W. and A . ) . C y t o l o g i a 3 8 ( 2 ) :
219-227. 578
A chromosome number of 2n - 22 was found
in 15 v a r i e t i e s of C. aajan and in
A. lineata. One p a i r of s a t e l l i t e d c h r o ­
mosomes was observed in 13 o u t of t h e 15
v a r i e t i e s ; P-958 d i d not have t h e s a t e l
l i t e s and NP-69 had a h e t e r o m o r p h i c p a i r
w i t h one member of t h e p a i r possessing a
s a t e l l i t e . Karyotype a n a l y s i s r e v e a l e d
c o n s i d e r a b l e i n t e r v a r i e t a l v a r i a t i o n regardi
n g arm r a t i o , t o t a l l e n g t h , and r a t i o o f
l o n g e s t and s h o r t e s t chromosomes. T h i s
v a r i a t i o n i s n o t a s s o c i a t e d w i t h any morp
h o l o g i c a l and agronomic c h a r a c t e r s of t h e
v a r i e t i e s , a l t h o u g h P-458, the v a r i e t y
w i t h o u t s a t e l l i t e s , was d i s t i n g u i s h e d
from the o t h e r v a r i e t i e s b y i t s obovate
t r i f o l i a t e l e a v e s . The chromosome morphol
o g y of A. lineata c l o s e l y resembled t h a t
of C. aajan T = 2 1 .
SIKDAR, A.K. 1965.
C y t o l o g i c a l i n v e s t i g a t i o n on Atylosia and
i t s h y b r i d i z a t i o n w i t h Cajanus. D I I T
(1965) T h e s i s . I n d i a n I n s t i t u t e o f Techn
o l o g y , Kharagpur, West B e n g a l ,
I n d i a . 579
SIKDAR, A . K . , and D.N. DE. 1967.
C y t o l o g i c a l s t u d i e s o f two species o f
Atylosia and Cajanus aajan. B u l l . B o t .
Soc. Beng. 2 1 ( 1 ) : 2 5 - 2 8 . 580
SINGH, S.P. 1947.
A new method of a p p l i c a t i o n of acenapht
h e n e . S c i . C u l t . 1 2 : 5 9 3 - 5 9 4 . 581
A method of t r e a t i n g seeds w i t h acenaphthene
i n l a r d i s o u t l i n e d . The e f f e c t s o f
acenaphthene t r e a t m e n t on Cajanus c e l l s are
d e s c r i b e d . I t i s thought t h a t chromosome
d o u b l i n g m i g h t be induced by v a r y i n g t h e
c o n c e n t r a t i o n of t h e acenaphthene and t h e
d u r a t i o n o f t h e t r e a t m e n t .
SINGH, VEDPAL. 1959.
R e t a r d a t i o n i n t h e r a t e o f g e r m i n a t i o n o f
Cajanus c a j a n ( L i n n ) M i l l s p . seeds t r e a t e d
w i t h c o l c h i c i n e . Agra U n i v . J . Res. ( S c i . )
8 ( 1 ) : 35-38. 582
SINHA, S . S . N . , and S.B. AKHAURY. 1969.
E f f e c t of gamma-radiation and E.M.S. on
Cajanus c a j a n . P r o c . I n d i a n S c i . Cong.
Assoc. 5 6 ( 3 ) : 3 4 8 - 3 4 9 . ( A b s t r a c t ) . 583
79