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P h y s i o l o g y
MUKHERJEE, D. 1974.
K e t o - a c i d s and amino a c i d s changes in
l e a v e s , f l o w e r s and f r u i t s o f Cajanus
c a j a n . J . I n d i a n B o t . Soc. 5 3 ( 1 - 2 ) :
115-118. 1181
C o r r e l a t i v e s t u d i e s o n changes i n k e t o acids
and f r e e amino a c i d s and amide have been
made i n l e a v e s , f l o w e r s , and f r u i t s o f
Cajanus c a j a n . The c o n c e n t r a t i o n of k e t o
a c i d s has been found to be much h i g h e r than
t h e q u a l i t y o f amino a c i d s i n these p a r t s .
These m e t a b o l i t e s , e s p e c i a l l y k e t o a c i d s ,
are r e a d i l y used u p d u r i n g t h e t r a n s f o r m a ­
t i o n o f v e g e t a t i v e i n t o r e p r o d u c t i v e phase
and o t h e r a c t i v e growth p e r i o d s o f t h e
p l a n t .
MULIMANI, V . H . , and MADAIAH. 1974.
S t u d i e s i n urease E C - 3 . 5 - 1 . 5 , d i s t r i b u t i o n
of Urease in p l a n t , seeds/Cajanus indicus,
Cuaumis s a t i v u s d r y imbibed seeds s y n t h e s i s .
J . Karnataka U n i v . 1 9 : 1 7 6 - 1 8 3 . 1182
NATH, R.L. 1960.
A c t i o n of urease from Cajanus indiaue on
b l o o d urea in vivo. J. P r o c . I n s t . Chem.
3 2 ( 4 ) : 1 6 5 - 1 6 7 . 1183
A h i g h l y s o l u b l e p r e p a r a t i o n was made of
urease from C. indiaue. T h i s p r e p a r a t i o n
when i n j e c t e d i n t r a v e n o u s l y i n t o dogs
showed t h a t t h e average l o w e r i n g of b l o o d
urea was about 28% in 45 m i n u t e s .
NATH, R . L . , and T.K. PRADHAN. 1960.
Note on t h e s t u d y of Urease from Cajanus
i n d i c u s ( A r h a r ) . B u l l . C a l c u t t a Sch. T r o p .
Med. 8 ( 2 ) : 5 9 - 6 0 . 1184
Urease was prepared from dehusked seeds by
v a r i o u s methods. The a c t i v i t y a t 37°C,
determined by t h e method of Nath and U l l a h ,
was almost 1.6 times h i g h e r than t h a t at
30°C. The h i g h e s t a c t i v i t y was o b t a i n e d
when e x t r a c t i o n was c a r r i e d o u t w i t h 40%
e t h a n o l . Products f r o m acetone p r e p a r a ­
t i o n s were more s o l u b l e i n w a t e r t h a n those
from e t h a n o l p r e p a r a t i o n s . A t l o w e r c o n ­
c e n t r a t i o n s t h e r e was d e v i a t i o n from t h e
f i r s t o r d e r l a w , which c o u l d b e e x p l a i n e d
by i n h i b i t i o n by NH 4 + from (NH 4 ) 2 CO3
formed b y t h e h y d r o l y s i s o f u r e a .
NATH, R . L . , and T.K. PRADHAN. 1960.
A s t u d y on urease f r o m Cajanus oajan L i n n .
M i l l s p . P a r t I I I . C r y s t a l l i z a t i o n . Ann.
Biochem. Exp Med. 2 0 ( 5 ) : 1 2 7 - 1 3 0 . 1185
E x t r a c t i o n o f t h e crude powder w i t h w a t e r ,
a c e t o n e , o r e t h a n o l f o l l o w e d b y p r e c i p i t a -
t i o n w i t h v a r i o u s c o n c e n t r a t i o n s o f e t h a n o l
o r acetone were t r i e d . Best c r y s t a l l i z a ­
t i o n was a c h i e v e d , b u t i n poor y i e l d , b y
e x t r a c t i o n w i t h 40% e t h a n o l and p r e c i p i t a ­
t i o n w i t h a n equal amount o f a b s o l u t e
e t h a n o l . The enzyme appears to be completel
y s p e c i f i c f o r u r e a .
NATH, R . L . , and T.K. PRADHAN. 1960.
A study on urease from Cajanus oajan. I V .
K i n e t i c s t u d y w i t h c r y s t a l l i n e u r e a s e .
Ann. Biochem. E x p l Med. 2 0 : 1 5 7 - 1 6 4 . 1186
The optimum pH was found to be 7.2 to 7.5
a t s u b s t r a t e c o n c e n t r a t i o n s o f 0 . 0 1 t o
0.05 M and 7.4 t o 7.6 a t 0.5 M. A pH
h i g h e r than optimum f a v o r s enzyme-substrate
complex f o r m a t i o n w h i l e lower pH f a v o r s
d e c o m p o s i t i o n o f the p r o d u c t s .
NATH, R . L . , and T.K. PRADHAN. 1964.
E f f e c t o f versene o n the a c t i v i t y o f urease
from red gram. Cajanus oajan. J. P r o c .
I n s t . Chem. 3 6 ( 3 ) : 1 8 8 - 1 9 1 . 1187
PALIWAL, K . V . , and G.L. MALIWAL. 1973.
S a l t t o l e r a n c e of some a r h a r (Cajanus indicus)
and cowpea (V. s i n e n s i s ) v a r i e t i e s at
g e r m i n a t i o n and s e e d l i n g s t a g e s . Ann.
A r i d Zone 1 2 ( 3 - 4 ) : 1 3 5 - 1 4 4 . 1188
The e f f e c t s o f d i f f e r e n t s a l i n i t y l e v e l s
at v a r i o u s c o n c e n t r a t i o n s of NaCl + CaCl2
on 23 C. indiaue v a r i e t i e s , and 13 v a r i e -
t i e s of V. sinensie. I n c r e a s e d s a l i n i t y
reduced g e r m i n a t i o n percentage and s e e d l i n g
h e i g h t i n a l l v a r i e t i e s b u t s i g n i f i c a n t
v a r i e t a l d i f f e r e n c e s i n s a l t t o l e r a n c e
were n o t e d .
PANDEY, R.K., and M.C. SAXENA. 1974.
M o r p h o l o g i c a l c o n s i d e r a t i o n s i n the development
o f e f f i c i e n t p l a n t types o f a r h a r
and gram. P r o c . 2nd General Cong. SABRA0,
New D e l h i , I n d i a . 1012-1015. 1189
The v e g e t a t i v e and r e p r o d u c t i v e phases in
i d e a l types of Cajanus c a j a n and Cicer
a r i e t i n u m are c o n s i d e r e d i n r e l a t i o n t o
p h o t o s y n t h e s i s and r e s p i r a t i o n . C. arietinum
i s a l s o c o n s i d e r e d i n r e l a t i o n t o f r o s t
and s a l i n i t y r e s i s t a n c e .
PANDEY, R . K . , M.C. SAXENA, M.H. KALUBARME,
V . B . SINGH, and V . V . S . S . PRASAD. 1976.
Genotypic v a r i a t i o n s i n p h o t o s y n t h e t i c r a t e
and r e s p i r a t o r y l o s s e s i n some g r a i n
legumes. P I . Biochem. J . 3 ( l ) : 7 2 - 8 0 . 1190
The r e l a t i v e r a t e of p h o t o s y n t h e s i s (RRP)
in Cajanus oajan c u l t i v a r s , as measured by
14 CO 2 f i x a t i o n ranged from 100% in 1 4 cv.
1
Pant A-3 to 126% in c v . UPAS-120.
4 CO 2
147