EMBO WORKSHOP 2005 - Istituto Pasteur
EMBO WORKSHOP 2005 - Istituto Pasteur
EMBO WORKSHOP 2005 - Istituto Pasteur
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
<strong>EMBO</strong> <strong>WORKSHOP</strong> <strong>2005</strong><br />
CHROMOSOME STRUCTURAL ELEMENTS: FROM DNA SEQUENCE TO FUNCTION<br />
Villa Mondragone, Monte Porzio Catone, Rome, Italy<br />
September 29-October 3, <strong>2005</strong><br />
Organizer:<br />
Fiorentina Ascenzioni, Dipartimento di Biologia Cellulare e dello Sviluppo, University of Rome<br />
“La Sapienza”, via dei Sardi 70, 00185 Rome, Italy<br />
Co-organizers:<br />
- Silvia Bacchetti, <strong>Istituto</strong> Regina Elena, via delle Messi d’Oro, 156, 00158 Rome, Italy<br />
- Giuseppe Novelli, Dipartimento di Biopatologia e Diagnostica, University of Rome Tor<br />
Vergata, via Montepellier 1, 00133 Rome, Italy<br />
- Maria Savino, Dipartimento di Genetica e Biologia Molecolare, University of Rome “La<br />
Sapienza”, P.le Aldo Moro 5, 00185 Rome, Italy<br />
Person to whom enquiries may be addressed: Fiorentina Ascenzioni,<br />
e-mail: fiorentina.ascenzioni@uniroma1.it<br />
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
Workshop Programme<br />
Talks (T) 25 min + 5 min discussion; Short Talks (ST): 12 min + 3 min discussion, P, Posters<br />
29/09/<strong>2005</strong> Thursday<br />
4-6 pm: Registration, hanging of posters<br />
6 pm: Welcoming remarks: Silvia Bacchetti/Fiorentina Ascenzioni<br />
6:15-7:15 pm: Lecture: Lucio Luzzatto (IT), Chromosomal and molecular basis of<br />
cancer.<br />
Cocktails<br />
30/09/<strong>2005</strong> Friday<br />
9.00-10.30am Session 1: Telomeres: structure, proteins and positioning<br />
Chair: P. Donini (IT)<br />
9:00 T1- Patrick Kaminker (USA), Dual function for a telomere-associated protein?<br />
9:30 T2- Daniela Rhodes (UK), Towards the structure of the “30 nm” chromatin fiber.<br />
10:00 ST1- Heico Schoiber (CH), Telomerase Helps Tether Telomeric Chromatin to the<br />
Nuclear Periphery via Interaction with yKu<br />
10.30-11.00am Coffee break<br />
11 am -12.30pm<br />
11:00 T3- Stefano Cacchione (IT), The role of DNA sequence in telomeric chromatin features<br />
11:30 T4- Hans Lipps (DE), Telomere end-binding proteins control the formation of G-<br />
quadruplex DNA structures in vivo.<br />
12:00 ST2- K. Lemke (Poland). Interaction of antitumor triazoloacridone C-1305 with guaninerich<br />
DNA: induction of structural changes in guanine triplets and stabilization of G-quadruplexes<br />
12:15 ST3- H. Renauld (USA), Interstitial (sub)telomeric repeats: Punctuation marks of genome<br />
dynamics?<br />
12.30-2pm Lunch<br />
2-4.30pm Session 2: Telomere replication and telomere length regulation<br />
Chair: Virginia Zakian (USA)<br />
2:00 T5- Virginia Zakian (USA), Pif1p family helicases: effects on telomerase.<br />
2:30 T6- Eric Gilson (FR), New pathways of telomere length regulation in yeast.<br />
3:00 T7- Arturo Londoňo-Vallejo (FR), Telomere homeostasis in humans: length control at<br />
cell and single telomere levels.<br />
3:30 ST4- S. Bekaert (Belgium), Reference values and basic determinants of telomere length<br />
in a large middle-aged population.<br />
3:45 ST5- D. Baird (UK), Telomere dynamics and instability.<br />
4:00 ST6- M.A. Cerone (Canada) A human cell line that maintains telomeres in the absence of<br />
telomerase and of key markers of ALT.<br />
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
4.15 ST7- C.M. Counter (USA) Loss of hPot1 function leads to telomere instability and a cutlike<br />
phenotype.<br />
4.30-5.00pm Coffee break<br />
5:00-7:15pm Session 3: Chromosome replication and disjunction<br />
Chair: Hans Lipps (DE)<br />
5:00 T8- Michelle Debatisse (FR), Dynamics of DNA replication in mammalian somatic cells<br />
5:30 T9- Silvano Riva (IT), Functional analysis of a human DNA replicator.<br />
6:00 T10- Susan Smith (USA), Telomeres require special mechanisms for their compaction<br />
and resolution at mitosis.<br />
6:30 ST8- Peter Meister (CH), Spatial and temporal separation of replication and<br />
recombination requires the intra-S checkpoint<br />
6:45 ST9- M. Cornfort (USA), Excess of Radiation-Induced Dicentrics Involving Homologous<br />
Chromosomes.<br />
7:00 ST10- B. Meier (USA), The C. elegans catalytic subunit of telomerase TRT-1 and DNA<br />
damage signaling.<br />
7.15 ST11- S. Bailey (USA), Telomeres and Double-Strand Breaks: Tying up Loose Ends.<br />
01/10/<strong>2005</strong> Saturday<br />
9.00-10.30am Session 4: Centromeres, DNA sequences and chromatin plasticity<br />
Chair: Brenda Grimes (USA)<br />
9:00 T11- Beth A. Sullivan (USA), Centromeric Chromatin is a Continuous, Dynamic<br />
Domain that Permits Gene Expression<br />
9:30 T12- Christine Farr (UK), Topoisomerase II: untangling its contribution at centromere<br />
10:00 T13- Giuliano Della Valle (IT), Dissecting Cenp-C function in mammalian centromeres<br />
10.30-11am Coffee break<br />
11.00am -12.15pm Session 4 continued<br />
11:00 T14- W.C. Earnshaw (UK), Genetic analysis of chromosome segregation in vertebrate<br />
cells.<br />
11:30 ST12- C. Desmaze (FR), Chromatin structure and dynamics in the formation of<br />
chromosome aberrations.<br />
11:45 ST13- C. Woo (USA), Long-range Nucleosome Mapping of the HOX Gene Cluster.<br />
12:00 ST14- J. Fajkus (CZ), Minisatellite telomeres in the family Alliacae are lost with<br />
divergence of the genus allium.<br />
12.30 –3.30 pm Poster session with Lunch<br />
3.30pm<br />
6pm<br />
7:30pm<br />
Departure for Rome by turist coach<br />
Guided visit to part of the Musei Capitolini and the Campidoglio (City Hall<br />
of Rome)<br />
Cocktails and dinner on the adjoining Terrazza Caffarelli<br />
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
02/10/05 Sunday<br />
9-10:30am Session 5: De novo centromeres assembly and Chromosome Engineering<br />
Chair: W.C. Earnshaw (UK)<br />
9:00 T15- Brenda Grimes (USA), Segregation of human artificial chromosomes in mouse<br />
cells.<br />
9:30 T16- T. Voet, (Belgium), Optimisation and exploitation of a chromosomal vector<br />
10:00 ST15- N. Mestrovic (Croatia), The MEL-172 satellite DNA library in parthenogenetic<br />
meloidogyne species: conserved pattern of nucleotide variability along the monomers<br />
11.30-11.00am Coffee break<br />
11.00am -12.30pm Session 5 continued<br />
Chair: Christine Farr (UK)<br />
11:30 T17- H. Masumoto (JPN), Chromatin assemblies required for de novo human artificial<br />
chromosome formation.<br />
12:00 T18- Fiorentina Ascenzioni (IT), Human minichromosomes: expression vectors for<br />
human genes.<br />
12.30-3pm Poster session with Lunch<br />
3.00-4.00pm Lecture: Giorgio Bernardi (IT), Chromatin structure in interphase nuclei of<br />
vertebrates and the formation GC-rich isochores.<br />
4:00-4.30pm Coffee break<br />
4.30-6:00pm session 6: Chromosomes organization and gene evolution<br />
Chair: Giuseppe Novelli (IT)<br />
4:30 T19- M. Cremer (DE), Nuclear architecture in the light of conserved higher order<br />
chromatin arrangements.<br />
5:00 T20- Mariano Rocchi (IT), Centromere repositioning in evolution.<br />
5:30 T21- Novelli Giuseppe (IT), The role of the nuclear envelope in genome organisation.<br />
Closing remarks: Silvia Bacchetti and Giuseppe Novelli<br />
2/10-<strong>2005</strong> or 3/10/05 Departure<br />
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
Posters*<br />
P1 - C. Auriche (IT), Chronic Chromosome Instability in Humanized Yeast<br />
P2 - C. Azzalin (CH), The human RNA surveillance factors UPF1 and EST1A/SMG6 are<br />
essential for S-phase progression and DNA repair.<br />
P3 - M. Babar (Pakistan), Identification of bacterial blight resistance genes Xa4 in Pakistani rice<br />
germplasm using PCR based markers.<br />
P4 - S. Carrabino (IT), Polyethylenamine/albumin complexes display high transfection<br />
efficiency in confluent and polarized human respiratory epithelial cells.<br />
P5 - I. Draskovic (FR), Molecular mechanisms of alternative lengthening of telomeres (ALT) in<br />
human cancers.<br />
P6 – A. Farsetti (IT), Primary cultures of prostate luminal cells as a model for an integrated<br />
approach to prostate cancer: isolation, characterization and gene profiling.<br />
P7 - M. Gezahagne (Ethiopia), Species distinctiveness between Trifolium quartinianum and<br />
Trifolium steudneri.<br />
P8- C. Keil (DE), Functional interactions of poly(ADP-Ribose)glycohydrolase with PARP-1 and<br />
XRCC1<br />
P9-– C. Lan (Taiwan), Significance of Topoisomerase 2a in association with Her2/neu in breast<br />
cancer management.<br />
P10- S-C. Liang (Taiwan), Chromosomal aberrations in malignant pleural effusion from lung<br />
adenocarcinoma: varying cytogenetic abnormalities detected in relationship to gender and<br />
smoking.<br />
P11 - L-C. Lin (Taiwan), EGFR gene sequence in breast cancer patients in Taiwan.<br />
P12 – C. Mondello (IT), Cellular transformation of telomerase immortalized human fibroblasts.<br />
P13 – B. Mravinac (Croatia), What a difference “junk” DNAs make…..<br />
P14 – V. Petrovic (Croatia), Intricate patterns of sequence divergence and conservation in an<br />
organizationally complex Donax trunculus satellite DNA family.<br />
P15 – G. Piaggio (IT), NF-Y dependent chromatin remodelling in proliferation and<br />
differentiation.<br />
P16 – S. Redon (FR), Protein-protein and protein-RNA interactions mediate the association of<br />
human EST1a with telomerase.<br />
P17 – E. Sykorova (CZ), Plant telomerases synthesizing human type of telomeres.<br />
* Poster will be displayed for the duration of the meeting. Each poster has been assigned a<br />
number according to the list above. Presenting authors are requested to stand alongside their<br />
poster during the poster sessions.<br />
Maximum size of the poster: 1.20cm high x 1.50 width cm<br />
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
Travelling to Villa Mondragone<br />
From Leonardo da Vinci-Fiumicino Airport<br />
1. Take the Express Train (Leonardo Express) to Rome Termini Station (about E8). The<br />
train leaves every 30 min (7 and 37 min after the hour) and takes about 30 min to reach<br />
the Termini Station.<br />
2. Take the local train to Frascati. Tickets for this train can be bought at the Airport Station.<br />
The train for Frascati leaves every hour beginning at 5:46am. Following trains are at:<br />
6:32am / 7:33am / 8:35am and then 52 min after the hour till 21:52pm.<br />
3. At the Frascati Station take a taxi to Villa Mondragone or Hotel Villa Vecchia.<br />
Notification of where you will be staying will be posted on the site at a later time.<br />
4. Alternatively, take a taxi from the airport to Villa Mondragone or Villa vecchia (about<br />
E60).<br />
Please make sure to discuss the fare at departure.<br />
From Ciampino Airport<br />
1. Take the shuttle bus (leaving every 20 min till 23:30pm) or a taxi to Ciampino Train<br />
Station. From there take the train to Frascati which leaves at: 4:49am / 6:02am / 6:50am/<br />
7:47am / 8.53am / 10:08am and then at 8 min after the hour till 22:08pm.<br />
2. From Frascati Station take a taxi to Villa Mondragone or Hotel Villa Vecchia.<br />
Notification of where you will be staying will be posted on the site at a later time.<br />
3. Alternatively take a taxi from Ciampino Airport to Villa Mondragone or Villa vecchia<br />
(about E25). Please make sure to discuss the fare at departure.<br />
Local Taxi companies<br />
Taxi Frascati: 06/9422500<br />
Alfredo Iacoangeli: Via G. Saragat, 2 00045 Genzano (RM) 328/3911794 06/9397592<br />
Andrea Amore: 338/8888550, 328/3332022, 340/7240146<br />
Migliori Gianni: 349/5860561<br />
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Chromosome Structural Elements/Rome <strong>2005</strong><br />
Venue<br />
Villa Mondragone<br />
Via Frascati 51, 0040 Monteporzio Catone, Rome<br />
Tel +39 06 9420018<br />
www.villamondragone.it<br />
info@villamondragone.it<br />
Accomodation<br />
Hotel Villa Vecchia,<br />
via Frascati 49, 0040 Monteporzio Catone, Rome<br />
tel +39 06 94340096, fax, +39 069420568<br />
www.villavecchia.it<br />
info@villavecchia.it<br />
Scientific Session<br />
All lectures will be held in Sala degli Svizzeri<br />
Poster will be displayed in Sala Riunioni<br />
Meals and Refreshments<br />
Coffee breaks and lunches will be served in Villa Mondragone<br />
Sponsors:<br />
Università degli Studi di Roma “La Sapienza”<br />
Università degli Studi di Roma Tor Vergata<br />
<strong>Istituto</strong> <strong>Pasteur</strong>-Fondazione Cenci Bolognetti<br />
Ministero dell’Istruzione, dell’Università e della Ricerca<br />
Applied Biosystems<br />
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