SELENITE CYSTINE BROTH (7283) - mibius
SELENITE CYSTINE BROTH (7283) - mibius
SELENITE CYSTINE BROTH (7283) - mibius
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YEPD AGAR (7294)<br />
Intended Use<br />
YEPD Agar is used for the maintenance and propagation of yeasts.<br />
Product Summary and Explanation<br />
YEPD Agar, Yeast Extract Peptone Dextrose Agar, is used often in yeast genetics studies for cultivating<br />
Saccharomyces cerevisiae and other yeasts. 1 Yeasts grow well on a minimal medium containing only<br />
dextrose and salts. The additional source of nitrogen and vitamins permits faster growth of yeasts.<br />
YEPD Agar is also referred to as YPD Agar, and is used extensively in molecular microbiology procedures. 2,3<br />
Principles of the Procedure<br />
Peptone provides the nitrogen and amino acids in YEPD Agar. Yeast Extract supplies vitamins and minerals<br />
to stimulate growth. Dextrose is the carbon energy source. Agar is the solidifying agent.<br />
Formula / Liter<br />
Yeast Extract .......................................................................... 10 g<br />
Peptone................................................................................... 20 g<br />
Dextrose ................................................................................. 20 g<br />
Agar ........................................................................................ 15 g<br />
Final pH: 6.5 ± 0.2 at 25°C<br />
Formula may be adjusted and/or supplemented as required to meet performance specifications.<br />
Precaution<br />
1. For Laboratory Use.<br />
Directions<br />
1. Dissolve 65 g of the medium in one liter of purified water.<br />
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.<br />
3. Autoclave at 121°C for 15 minutes.<br />
Quality Control Specifications<br />
Dehydrated Appearance: Powder is homogeneous, free flowing, and beige to yellow-beige.<br />
Prepared Appearance: Prepared medium is trace to slightly hazy, and light to yellow beige.<br />
Expected Cultural Response: Cultural response on YEPD Agar at 25 - 30°C and examined for growth at<br />
18 – 72 hours of incubation.<br />
Microorganism<br />
Kluyveromyces lactis ATCC® 8563<br />
Saccharomyces cerevisiae ATCC® 18790<br />
Saccharomyces cerevisiae ATCC® 9080<br />
The organisms listed are the minimum that should be used for quality control testing.<br />
Response<br />
growth<br />
growth<br />
growth<br />
Test Procedure<br />
Refer to appropriate references for specific procedures on the maintenance and propagation of yeasts. 1,2,3<br />
PI7294, Rev New, 11/06
Results<br />
After sufficient incubation, the medium should show growth as evidenced by formation of isolated colonies<br />
and/or a confluent lawn of growth on the surface of the agar.<br />
Storage<br />
Store sealed bottle containing the dehydrated medium at 2 - 30°C. Once opened and recapped, place<br />
container in a low humidity environment at the same storage temperature. Protect from moisture and light by<br />
keeping container tightly closed.<br />
Expiration<br />
Refer to expiration date stamped on the container. The dehydrated medium should be discarded if not free<br />
flowing, or if the appearance has changed from the original color. Expiry applies to medium in its intact<br />
container when stored as directed.<br />
Limitation of the Procedure<br />
1. Due to nutritional variation, some strains may be encountered that grow poorly or fail to grow on this<br />
medium.<br />
2. If adding supplements such as amino acid mixtures, care should be taken to avoid agar hydrolysis. Add<br />
the supplements after autoclaving and do not re-melt.<br />
Packaging<br />
YEPD Agar Code No. 7294A 500 g<br />
7294B 2 kg<br />
7294C 10 kg<br />
References<br />
1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1994. Current protocols in<br />
molecular biology, vol. 1. Current Protocols, New York, N.Y.<br />
2. Adams, A., D. E. Gottschling, C. A. Kaiser, and T. Stearns. 1997. Methods in yeast genetics: A Cold Spring Harbor Laboratory<br />
Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.<br />
3. Burke, D., D. Dawson, and T. Stearns. 2000. Method in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring<br />
Harbor, New York.<br />
Technical Information<br />
Contact Acumedia Manufacturers, Inc. for Technical Service or questions involving dehydrated culture media preparation or<br />
performance at (517)372-9200 or fax us at (517)372-2006.<br />
PI7294, Rev New, 11/06