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Media Components ISO 9001Certified ISO 13485 Certified Animal-free Reagents Minimize risks and improve consistency in cell-culture and production applications with Non-Animal Origin (NAO) reagents Animal-free LB Media • Superior performance - Bacterial growth curves match or exceed those obtained in conventional media. • Safer - Animal-free Peptone derived from soy meal • Convenient - Each package prepares one liter of media All components of AMRESCO’s animal-free LB Media are derived from non-animal origins and have been extensively tested to ensure that growth matches or exceeds that in media containing peptones from conventional animal sources. Supplied as a convenient, pre-mixed powder, animal-free LB media is a general purpose media for the growth of non-fastidious microorganisms. It may also be used as a base for enriched media when supplemented with additional growth requirements. Animal-free LB Media Components Final Amount (g/L) Soy Peptone, animal-free 10 Yeast extract 5 Sodium Chloride 5 Supplied as 5 individual packs per box. Each pack makes 1 L of media. Animal-free Peptone Excellent source of essential nutrients for the culture of microorganisms. Animal-free Peptone is a papaic/pancreatic digest of defatted soybean meal derived from sources free of genetic modifications. It is an ideal substitute for peptone derived from animal sources for all growth media including tissue culture, fermentation and agar plates. PRODUCT DESCRIPTION CODE SIZE LB Powder, Non-Animal Origin Each pack prepares 1 liter of broth Soy Peptone, Animal-free, GMO-free N526-5PK N454-100G N454-500G 5 Packs 100 g 500 g Comparision of Bacterial Growth in animal-free LB media (Code: N526) versus conventional LB media. Saturated cultures of E. Coli, P. aeruginosa, and S aureus were used to inoculate fresh cultures of either animal-free LB media or conventional LB media, (Miller Formulation, Code: J106), for 8 hours. <strong>Culture</strong>s were incubated at 37˚C with shaking. Growth curves were obtained by determining OD 600 at hourly intervals. 600 4
www.amresco-inc.com Media Components Animal-free Products Demand for animal-free reagents continues to rise in both research and production applications. <strong>Cell</strong> culture, theraputics and production processes that must be free of contamination by transmissible pathogens such as bovine spongiform encephalopathy (BSE) increasingly require development with animal-free components. Elimination of animal derived products can confer substantial cost and logistic benefits including: • Minimization of potential contamination by transmisible pathogens such as BSE • Reduction of costs related to logistics of animal derived products • Improvement of process consistency from pilot development to industrial production AMRESCO offers a variety of high quality, cost effective animal-free reagents that can replace animal derived counterparts including amino acids, protease inhibitors and media components. The animal-free products are obtained from a range of sources including: • Microbial fermentation • Plants • Synthetic processes The purity and consistency of each product is ensured by rigorous quality control testing performed on all AMRESCO products. Animal-free amino-acids High Purity For media Preparation Code Product 0106 L-Alanine 0877 L-Arginine 94341 L-Asparagine 0192 L-Aspartic Acid N554 L-Cysteine 0421 L-Glutamic Acid 0374 L-Glutamine 0167 L-Glycine E806 L-Histidine E803 L-Isoleucine E811 L-Leucine 0437 L-Lysine E801 L-Methionine 0991 L-Phenylalanine E812 L-Proline 1B1103 L-Serine E808 L-Threonine E800 L-Tryptophan N553 L-Tyrosine 1B1102 L-Valine Prices and specifications for the complete line of animal-free products offered by AMRESCO can be found in the current catalog or on our website at www.amresco-inc.com. Animal-free protease inhibitors superior Quality • Excellent Value Code Product M182 AEBSF M181 Antipain Dihydrochloride M141 Benzamidine Hydrochloride M179 Bestatin M184 Chymostatin M186 E-64 M101 EDTA M180 Leupeptin M183 Pepstatin M135 1,10-Phenanthroline M145 PMSF M185 Phosphoramidon 5 3
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Cell Culture

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