ABCD ABCD - Molecular Devices

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Assay statistics ABCD Z' 0.6-0.7 S/B ~11:1 Ralf Heilker Dep. of Integrated Lead Discovery Quality control software: batch stability over half an assay day ABCD Ralf Heilker Dep. of Integrated Lead Discovery
Quality control software: batch statistics ABCD 200%CTL: full activation of GPCR X1 by reference agonist 100%CTL: no activation of GPCR X1 using buffer Ralf Heilker Dep. of Integrated Lead Discovery Primary screen ABCD 100 % CTL: inactive compound 200 % CTL: reference agonist stimulated Hit threshold • ~750.000 compounds • Hit threshold >150 %CTL • ~23000 primary hits (hit rate of 3.1 %) Ralf Heilker Dep. of Integrated Lead Discovery
Page 1 and 2: ABCD Molecular Devices 8th Internat
Page 3 and 4: Integration of a 2nd FLIPR to an au
Page 5: Sealing of the compound dilution pl
Page 9 and 10: Host cell counterscreen: no compoun
Page 11 and 12: Counterscreen 2 ABCD I 0 hit antago
Page 13 and 14: GPCR X1 agonist ABCD EC50 0.457 ug/
Page 15 and 16: GPCR X1 no dose response curve fit
Page 17 and 18: Validate cAMP-AlphaScreen for Gs an

ABCD ABCD - Molecular Devices

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