ABCD ABCD - Molecular Devices

More documents

Recommendations

Info

Selectivity profile of "potent and efficient" GPCR X1 hits vs GPCR X2/X3 ABCD EC 50 (X1) [µM] No Effect on Subtype X2 No Effect on Subtype X3 Selectivity_X2 Selectivity_X3 No Effect on Subtypes X2 or X3 Ralf Heilker Dep. of Integrated Lead Discovery Functional secondary assays for the FLIPR format: cAMP-AlphaScreen ABCD GPCR α γ β ATP adenylate cyclase ↑ Fsk cAMP 680 nm 520-620 nm biotin-cAMP Acceptor-Anti-cAMP Donor-SA Ralf Heilker Dep. of Integrated Lead Discovery
Validate cAMP-AlphaScreen for Gs and Gi pathway in GPCR X1-transfected CHO cells ABCD Ralf Heilker Dep. of Integrated Lead Discovery Functional secondary assays for the FLIPR format: IP3-AlphaScreen ABCD Ralf Heilker Dep. of Integrated Lead Discovery
Page 1 and 2: ABCD Molecular Devices 8th Internat
Page 3 and 4: Integration of a 2nd FLIPR to an au
Page 5 and 6: Sealing of the compound dilution pl
Page 7 and 8: Quality control software: batch sta
Page 9 and 10: Host cell counterscreen: no compoun
Page 11 and 12: Counterscreen 2 ABCD I 0 hit antago
Page 13 and 14: GPCR X1 agonist ABCD EC50 0.457 ug/
Page 15: GPCR X1 no dose response curve fit

ABCD ABCD - Molecular Devices

Create successful ePaper yourself

Delete template?

Save as template?