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Acoelomates: Phylum Platyhelminthes and Nemertea - Biosciweb.net

Acoelomates: Phylum Platyhelminthes and Nemertea - Biosciweb.net

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<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.1<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

This lab is designed to explore the basic life history, physiology <strong>and</strong> adaptations of the<br />

acoelomate <strong>and</strong> pseudocoelomate animals with a focus on the Phyla Playthelminthes, <strong>Nemertea</strong><br />

(Rhynchocoela), Nematoda, Rotifera. We will review the lifecycles of many of these species<br />

with an emphasis on their commonly parasitic nature. You must learn these lifecycles.<br />

Refer to chapters in the textbook for illustrations, diagrams, <strong>and</strong><br />

additional information about acoelomates & pseudocoelomates.<br />

The Acoelomate Animals<br />

The acoelomates are animals that have no coelom (body cavity). They include flatworms,<br />

phylum <strong>Platyhelminthes</strong> (Gr. platys, flat, + helmins, worm); ribbon worms, phylum <strong>Nemertea</strong><br />

(Gr. Nemertes, one of the Nereids, mermaids of Greek mythology); <strong>and</strong> jaw worms, phylum<br />

Gnathostomulida (Gr. gnathos, jaw, + stoma, mouth). In acoelomate animals, the space<br />

between the body wall (ectoderm) <strong>and</strong> digestive tract (endoderm) is not a cavity, as in<br />

coelomate animals, but is filled with muscle fibers <strong>and</strong> a loose tissue of mesenchymal<br />

origin, called parenchyma, both derived from mesoderm. The presence of a well-developed<br />

mesodermal layer makes the acoelomates triploblastic (having three germ layers: ectoderm,<br />

endoderm, <strong>and</strong> mesoderm). Flatworms are a large <strong>and</strong> economically important group because<br />

they include not only free-living planarians but also parasitic tapeworms <strong>and</strong> flukes.<br />

<strong>Acoelomates</strong> are more complex in organization than the radiate animals in several ways:<br />

‣ <strong>Acoelomates</strong> have bilateral symmetry<br />

‣ They have defined tissues organized into functional organs<br />

‣ They have a highly organized nervous system with concentration of nervous tissue <strong>and</strong><br />

sense organs in the anterior end (cephalization)<br />

‣ They have an excretory system of specialized flame cells <strong>and</strong> tubules for elimination of<br />

nitrogenous wastes (breakdown products of protein, nucleic acid <strong>and</strong> other metabolism)<br />

‣ <strong>Platyhelminthes</strong> have a gastrovascular system, but ribbon worms have separated the<br />

two functions <strong>and</strong> have a complete mouth-to-anus digestive tract <strong>and</strong> a circulatory system<br />

‣ Flatworms have a tissue-organ level of organization<br />

Body Plan Features Retained by <strong>Acoelomates</strong> (seen in previously studied phyla):<br />

‣ true tissues present<br />

Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

(not seen in the <strong>Phylum</strong> Cnidaria – some of these traits are seen in other animal phyla in<br />

addition to the <strong>Phylum</strong> <strong>Platyhelminthes</strong>):<br />

1. acoelomate<br />

2. bilateral symmetry<br />

3. triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />

4. some degree of cephalization = head with sensory organs<br />

5. more organ systems (simple <strong>and</strong> formed of true tissues) are present<br />

6. flame cells in flame bulbs for excretion<br />

7. gastrovascular cavity for digestion <strong>and</strong> gas exchange (organs near body surface)<br />

8. longitudinal, circular <strong>and</strong> ventro-dorsal muscles<br />

9. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />

10. high surface-volume ratio as a result of flat shape (Important in locomotion <strong>and</strong> in<br />

exchange of nutrients <strong>and</strong> wastes).<br />

11. monoecious (hermaphroditic) = male <strong>and</strong> female organs in one individual<br />

12. most are parasitic, but some are free-living


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.2<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

Classification & Adaptations of Acoelomate Animals<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong> (flatworms)<br />

Class Turbellaria (tur'bel-lar' e-a) (L. turbellae, stir, bustle, + aria, like or connected with).<br />

About 3000 species; Turbellarians. Mostly free-living, with a ciliated epidermis. A paraphyletic<br />

grouping. Example: Dugesia tigrina<br />

Class Monogenea (mon'o-gen'e-a) (Gr. mono, single, + gene, origin, birth). About 1100 species;<br />

Monoge<strong>net</strong>ic flukes. Adult body covered with syncytial tegument without cilia; leaflike to<br />

cylindrical in shape; posterior attachment organ with hooks, suckers, or clamps, usually in<br />

combination; all parasitic, mostly on skin or gills of fishes; single host; monoecious; usually<br />

free-swimming ciliated larva. Examples: Polystoma, Gyrodactylus<br />

Class Trematoda (trem'a-to'da) (Gr. trematodes, with holes, + eidos, form). About 10,000<br />

species; Dige<strong>net</strong>ic flukes. Adult body covered with nonciliated syncytial tegument; leaf-like or<br />

cylindrical in shape; usually with oral <strong>and</strong> ventral suckers, no hooks; development indirect,<br />

first host a mollusc, final host usually a vertebrate; parasitic in all classes of vertebrates.<br />

Examples: Fasciola, Clonorchis, Schistosoma<br />

Class Cestoda (ses-to'da) (Gr. kestos, girdle, + eidos, form). About 3500 species; Tapeworms.<br />

Adult body covered with non-ciliated, syncytial tegument; scolex with suckers or hooks,<br />

sometimes both, for attachment; long, ribbonlike body, usually divided into series of<br />

proglottids; no digestive organs; parasitic in digestive tract of all classes of vertebrates; first<br />

host may be invertebrate or vertebrate. Examples: Taenia, Diphyllobothrium<br />

<strong>Phylum</strong> <strong>Nemertea</strong> or Rhynchocoela (ribbonworms)<br />

About 600 species. Ribbon worms are often called nemertine or nemertean worms. Nearly all<br />

are marine <strong>and</strong> are characterized by an eversible proboscis that can be thrown out with great<br />

speed to capture food. Ribbon worms have separated the two functions of the gastrovascular<br />

cavity in flatworms <strong>and</strong> have a complete mouth-to-anus digestive tract <strong>and</strong> a circulatory<br />

system. <strong>Nemertea</strong>ns occur in the intertidal, under stones or sea weed, or in burrows in muddy<br />

s<strong>and</strong>. <strong>Nemertea</strong>ns can be very long at up to 50 meters (these species are the worlds<br />

longest animals).<br />

Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> <strong>Nemertea</strong><br />

(some of these traits are seen in other animal phyla in addition to the <strong>Phylum</strong> <strong>Nemertea</strong>):<br />

1. acoelomate, bilateral symmetry, triploblastic structure<br />

2. some degree of cephalization = head with sensory organs<br />

3. some organ systems (formed of true tissues) are present<br />

4. eversible proboscis (only worm phylum with this characteristic)<br />

5. complete digestive tract/system (from mouth-to-anus)<br />

6. separate circulatory system with two lateral vessels on each side of the gut <strong>and</strong><br />

sinuses around head <strong>and</strong> tail; no definite closed circulation<br />

7. flame cells for excretion<br />

8. longitudinal <strong>and</strong> circular muscles contract against fibrous basement membrane;<br />

multiple patterns of muscle fiber organization<br />

9. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />

10. dioecious with sexual reproduction or asexual fragmentation<br />

11. not parasitic, free-living


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.3<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

The Pseudocoelomate Animals<br />

All bilateral animal phyla except the acoelomates possess a body cavity belonging to one of<br />

two types: (1) true coelom, in which a peritoneum (an epithelium of mesodermal origin)<br />

covers both the inner surface of the body wall <strong>and</strong> the outer surface of the visceral<br />

organs in the cavity, or (2) pseudocoel, a body cavity not entirely lined with peritoneum (one<br />

layer of mesoderm attached to the ectoderm).<br />

A body cavity of either type is an advantage because it provides room for organ development<br />

<strong>and</strong> storage <strong>and</strong> allows some freedom of movement within the body. The cavity is often fluidfilled<br />

<strong>and</strong> provides for a hydrostatic skeleton in those forms lacking a true skeleton.<br />

There are nine pseudocoelomate phyla, of which phylum Nematoda is by far the largest. All<br />

pseudocoelomate phyla are at the organ-system level of organization.<br />

In general pseudocoelomates tend to be cylindrical in body form, to be unsegmented, <strong>and</strong> to<br />

have a complete (mouth-to-anus) digestive tract (this is absent in acanthocephalans). The<br />

epidermis is usually covered with a cuticle. There are both aquatic <strong>and</strong> terrestrial members,<br />

<strong>and</strong> parasitism is fairly common.<br />

Body Plan Features Retained by Pseudocoelomates (seen in previously studied phyla):<br />

1. presence of true tissues<br />

2. bilateral symmetry<br />

3. triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />

4. some degree of cephalization = head with sensory organs<br />

5. some organ systems (formed of true tissues) are present<br />

Body Plan Features & Characteristics of Pseudocoelomates:<br />

1. possess a pseudocoelom (mesoderm lines ectoderm tissue side only)<br />

2. they are unsegmented<br />

3. complete digestive tract/system (from mouth-to-anus)<br />

4. epidermis is covered with a cuticle


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.4<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

Classification & Adaptations of Pseudocoelomate Animals<br />

<strong>Phylum</strong> Nematoda (roundworms)<br />

More than 25,000 species. Roundworms. Nematodes are an extensive group with worldwide<br />

distribution. They include terrestrial, freshwater, marine, <strong>and</strong> parasitic forms. They are<br />

elongated roundworms covered with a flexible, nonliving cuticle. Circular muscles are lacking<br />

in the body wall <strong>and</strong>, in Ascaris, longitudinal muscles are arranged in four groups separated by<br />

epidermal cords (some nematodes have six or eight groups of longitudinal muscles). Cilia are<br />

completely lacking. Nematodes are found free-living in almost every conceivable habitat from<br />

arid deserts to lake bottoms, rivers, polar seas. Nematodes - both parasitic <strong>and</strong> freeliving - are<br />

incredibly abundant. A h<strong>and</strong>ful of good garden soil contains thous<strong>and</strong>s of nematodes. Some<br />

50 different species of nematodes occur in humans, most of them nonpathogenic. Some<br />

nematodes are plant parasites feeding on plant sap, especially roots.<br />

Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> Nematoda<br />

(some of these traits are seen in other animal phyla):<br />

1. pseudocoelomate<br />

2. bilateral symmetry<br />

3. triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />

4. psuedocoelomate = possess a pseudocoelom<br />

5. some degree of cephalization = head with sensory organs<br />

6. some organ systems (formed of true tissues) are present<br />

7. vermiform (worm-like) body shape<br />

8. complete digestive tract with three angled (longitudinal, circular <strong>and</strong> oblique)<br />

muscular pharynx, intestine lacking muscular walls, short rectum <strong>and</strong> anus<br />

9. excretion <strong>and</strong> osmoregulation through the cuticle <strong>and</strong> by excretory canals<br />

10. lack both cilia <strong>and</strong> flame cells/bulbs<br />

11. body wall lined with cuticle which retains body shape<br />

12. longitudinal muscles only, no circular muscles in body wall; body length or<br />

diameter does not change in movement<br />

13. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />

14. dioecious; sexes separate with females larger than males; one or two tubular gonads;<br />

copulatory spicules present in males<br />

<strong>Phylum</strong> Rotifera (rotifers)<br />

There are about 2000 species of rotifers. The name "rotifer" is derived from the Latin word<br />

meaning "wheel-bearer"; making reference to the crown of cilia around the mouth of the rotifer.<br />

The rapid movement of the cilia in some species makes them appear to whirl like a wheel.<br />

Rotifers can be found in many freshwater environments <strong>and</strong> in moist soil. The habitat of<br />

rotifers may include still water environments, such as lake bottoms, as well as flowing water<br />

environments, such as rivers or streams. Rotifers are also commonly found on mosses <strong>and</strong><br />

lichens growing on tree trunks <strong>and</strong> rocks, in rain gutters <strong>and</strong> puddles, in soil or leaf litter, on<br />

mushrooms growing near dead trees, in tanks of sewage treatment plants, <strong>and</strong> even on<br />

freshwater crustaceans <strong>and</strong> aquatic insect larvae. Most species of rotifers are about 200 to 500<br />

micrometers long. However a few species, such as Rotaria neptunia may be longer than a<br />

millimeter. Rotifers are thus multicellular creatures who make their living at the scale of<br />

unicellular protists. Characteristic features of rotifers include the ciliated corona ("wheel<br />

organ") <strong>and</strong> the mastax. The corona is located anteriorly <strong>and</strong> functions in locomotion <strong>and</strong> food<br />

gathering. The corona is modified extensively in some species. The mastax is a muscular<br />

pharynx containing a complex set of hard jaws or trophi <strong>and</strong> is found in all rotifers. These<br />

characteristics of these structures have been used extensively in classifying rotifers.


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.5<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> Rotifera<br />

(some of these traits are seen in other animal phyla):<br />

1. bilateral symmetry <strong>and</strong> triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />

2. psuedocoelomate = possess a pseudocoelom<br />

3. some degree of cephalization = head with sensory organs<br />

4. some organ systems (formed of true tissues) are present<br />

5. complete digestive system with mouth <strong>and</strong> anus<br />

6. well muscularized pharynx = mastax that contains trophi (jaws) for sucking <strong>and</strong><br />

grinding food particles<br />

7. ciliated corona helps sweep food particles to mouth<br />

8. pair of protonephridial tubules for osmoregulation & excretion<br />

9. syncitial (multinucleate) epidermis; some have a secreted cuticle<br />

10. longitudinal muscles <strong>and</strong> circular muscles in body wall<br />

11. pedal gl<strong>and</strong>s of foot secrete adhesive material in both sessile <strong>and</strong> creeping forms<br />

12. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />

13. dioecious; sexes separate with females larger than males; one or two tubular gonads;<br />

copulatory spicules present in males; can be parthenogenic<br />

‣ Reproduction in Rotifers<br />

‣ single (male) or double set of gonads (female) <strong>and</strong> ducts in each sex<br />

‣ sexes separate (dioecious)<br />

‣ males unknown in class Bdelloidea <strong>and</strong> only occur a few weeks a year in the class<br />

Monogononta<br />

‣ all females are parthenoge<strong>net</strong>ic in the class Bdelloidea (diploid females produce<br />

diploid females)<br />

‣ parthenogenesis = unisexual reproduction involving the production of young by<br />

females that are not fertilized by males; common in rotifers, aphids, bees, ants <strong>and</strong><br />

wasps. Parthenoge<strong>net</strong>ic eggs may be haploid or diploid.<br />

‣ in the class Monogononta, most of the year, diploid females produce thin shelled<br />

diploid amictic eggs. These amictic eggs develop parthenoge<strong>net</strong>ically into diploid<br />

(amictic) females. When the environment in the pond or stream changes due to<br />

crowding, diet, change in photoperiod, etc. some amictic eggs develop into diploid<br />

mictic females that produce thin shelled haploid eggs. If these eggs are not fertilized<br />

then they will develop into haploid males. If the eggs are fertilized, they become<br />

mictic eggs with a thick, resistant shell <strong>and</strong> become dormant. These “winter eggs”<br />

can survive until the environment becomes more suitable at which time they hatch <strong>and</strong><br />

develop into amictic females (completing the cycle). Females grow <strong>and</strong> reach maturity<br />

in a few days while males rarely grow <strong>and</strong> are mature at birth.


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.6<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ Concepts Related to Parasitic Worms<br />

Basic Requirements for a Parasitic Life<br />

1. The parasite must have or obtain access to a host(s)<br />

2. The parasite must establish itself <strong>and</strong> survive within the host(s)<br />

3. The parasite must show successful reproduction <strong>and</strong> transmission to a new host<br />

Special Parasitic Adaptations<br />

1. Invasion of the host can occur through many mechanisms:<br />

a. through the mouth with food <strong>and</strong>/or water<br />

b. through the skin by pe<strong>net</strong>ration<br />

c. through a vector such as a biting insect<br />

2. Establishment <strong>and</strong> survival within a host requires:<br />

a. transport of parasites/larvae/eggs in host through blood or lymph<br />

b. resistance to host's defenses (such as resistance to immune cells, circulating<br />

antibodies <strong>and</strong> acids <strong>and</strong> enzymes in the digestive tract for intestinal parasites)<br />

3. Reproduction, by cross fertilization:<br />

a. most trematodes <strong>and</strong> cestodes are monoecious (both sexes in one individual)<br />

b. most nematodes are dioecious; generally, males are smaller than females<br />

4. Parasitic Worm Life Cycles<br />

SEE THE DETAILED HANDOUT AVAILABLE ON THE COURSE WEBSITE<br />

a. Fertilization occurs within a host <strong>and</strong> fertilization is internal as a result of<br />

copulation. The parasite zygote develops into a shelled embryo which develops<br />

into a larval stage(s).<br />

b. SIMPLE PATTERN (monogenes, some tapeworms, nematodes)<br />

ZYGOTE -----> LARVA -----> ADULT -----> MANY EGGS<br />

c. COMPLEX PATTERN (digenes <strong>and</strong> some tapeworms)<br />

ZYGOTE --> 1st LARVAL STAGE--> 2nd LARVAL STAGE--><br />

3rd LARVAL STAGE --> ADULT --> MANY EGGS<br />

5. Dige<strong>net</strong>ic trematodes <strong>and</strong> cestodes have specialized surfaces consisting of a<br />

cellular syncytium with microvilli (microtrichs) which serve to increase the<br />

absorptive surface up to 50 times. Membrane surface serves to protect against<br />

host's digestive enzymes, serves in chemical modification of absorbed<br />

solutes, <strong>and</strong> serves in membrane transport


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.7<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ LIFECYCLES OF HELMINTHS (Worms)<br />

‣ TREMATODES (Class Trematoda)<br />

Clonorchis sinensis = The oriental liver fluke<br />

HOST (human)<br />

adult worm in liver<br />

shelled embryo<br />

miracidium<br />

SNAIL<br />

FISH<br />

metacercaria<br />

(in muscle)<br />

cercaria<br />

redia<br />

sporocyst<br />

Schistosoma mansoni -- Blood fluke<br />

HOST (human)<br />

adult worm in<br />

liver<br />

shelled embryo<br />

miracidium<br />

cercaria<br />

sporocyst<br />

SNAIL<br />

‣ CESTODES (Class Cestoda)<br />

Taeniarhynchus saginatus - The beef tapeworm<br />

ADULT HOST (humans)<br />

adult worm in intestine<br />

shelled embryos<br />

in proglottid<br />

consumed with<br />

grass by cattle<br />

cysticercus (bladder worm)<br />

in beef muscle<br />

onchospheres<br />

in blood


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.8<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ NEMATODES (<strong>Phylum</strong> Nematoda)<br />

Ascaris lubricoides = intestinal roundworm<br />

Ascaris lumbricoides -- Intestinal roundworm<br />

Adults (male & female)<br />

in human intestine<br />

shelled embryos<br />

in feces<br />

shelled larvae<br />

develop (10-15<br />

days) on soil.<br />

in lung, larvae leave<br />

blood, move to<br />

trachea, esophagus<br />

larvae emerge in<br />

duodenum, pe<strong>net</strong>rate gut<br />

wall <strong>and</strong> enter blood stream<br />

shelled larvae<br />

ingested by humans<br />

with food or water<br />

Ancylostoma duodenale = hookworm<br />

Ancylostoma duodenale -- Hookworm<br />

Adults in human<br />

intestine<br />

thin shelled embryos in feces<br />

larvae develop,<br />

hatch in soil<br />

esophagus<br />

larvae grow,<br />

feed on feces<br />

bronchi<br />

in lung, larvae leave<br />

blood, enter alveoli<br />

larvae attach,<br />

pe<strong>net</strong>rate skin,<br />

enter blood stream<br />

Enterobius vermicularis = pinworm<br />

Enterobius vermicularis -- Pinworm<br />

Adults (male & female)<br />

in human large intestine<br />

<strong>and</strong> cecum<br />

females migrate to<br />

anus at night<br />

deposited shelled<br />

embryos cause itching<br />

hatch in duodemum<br />

reinfection of self,<br />

others, water <strong>and</strong> food.<br />

Also in air or dust.<br />

fingernails <strong>and</strong><br />

clothing pick up<br />

shelled embryos


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.9<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

LAB PROCEDURE<br />

NAME:<br />

LAB SCORE:<br />

Refer to the textbook <strong>and</strong> the inter<strong>net</strong> to help you answer the questions.<br />

You must answer ALL questions in the lab procedure for full credit.<br />

Finish them at home if you do not have time to complete them in lab.<br />

Answer these questions:<br />

List some advantages of bilateral symmetry over radial symmetry?<br />

What advantages does cephalization offer for a bilateral animal?<br />

<strong>Acoelomates</strong> <strong>and</strong> pseudocoelomates have excretory systems of specialized flame cells or<br />

protonephridia for the elimination of nitrogenous wastes. How does a radiate animal, such as<br />

a hydra or sea anemone, rid itself of waste molecules?<br />

Cilia are completely lacking in nematodes. Are cilia present in any acoelomates? If so,<br />

during what life stage are the cilia often obvious?<br />

Are cilia present in any cnidarians?


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.10<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Turbellaria = free-living flatworms<br />

‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />

‣ Record the descriptive information requested at the end of the lab for each species.<br />

‣ Dugesia sp., a triclad flatworm<br />

‣ an available polyclad flatworm<br />

‣ What are the two major orders of flatworms in the Class Turbellaria?<br />

‣ Observations of a Free-Living Planarian Flatworm<br />

‣ Obtain a living specimen of Dugesia tigrina or Planaria sp. as available in a small<br />

petri dish or watch glass <strong>and</strong> study it under the dissecting microscope.<br />

Species name of your observed specimen:<br />

‣ Note the gliding movement. What causes it?<br />

‣ Using forceps, gently turn your flatworm over. Observe <strong>and</strong> describe the righting response.<br />

‣ How long does the righting response take?<br />

‣ Does the worm avoid obstacles such as a needle or probe without touching them?<br />

‣ Locate the eyespots <strong>and</strong> the “ears” or auricular organs. These organs contain<br />

chemoreceptors for detection of specific molecules (eg. “smell”/olfaction). Place a small<br />

piece of liver in the dish <strong>and</strong> observe <strong>and</strong> record how the worm responds.


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.11<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ Do you think the response to the liver depends on the presence of the auricular organs?<br />

‣ Briefly describe how you could test your hypothesis?<br />

‣ Does the worm respond to differences in light intensity? Record your observations below.<br />

‣ Record the reaction of the planarian to the addition of one drop of a 0.1% salt solution<br />

placed in front of it.<br />

‣ How do the two major types of turbellarian (Class Turbellaria) flatworms differ? (eg. How do<br />

members of the Order Tricladida (triclad flatworms) differ from members of the Order<br />

Polycladida (polyclad flatworms – how are their bodies different)?


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.12<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ Observations of Planarian Flatworm Body Structures<br />

‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />

Dugesia sp.or Planaria sp.<br />

‣ Draw <strong>and</strong> CLEARLY label the Dugesia sp.or Planaria sp. whole mount.<br />

‣ eyespots<br />

‣ intestine<br />

‣ auricular organs<br />

‣ pharynx<br />

‣ Draw <strong>and</strong> CLEARLY label the Dugesia sp.or Planaria sp. cross section through the<br />

pharynx.<br />

‣ intestine<br />

‣ diverticula<br />

‣ epidermis<br />

‣ pharynx<br />

‣ cilia<br />

‣ nerve cords<br />

‣ circular muscles<br />

‣ longitudinal muscles<br />

‣ Planaria Regeneration Experiments<br />

‣ Your instructor may perform incisions on some planarian flatworms to demonstrate<br />

regeneration. We will culture <strong>and</strong> observe these specimens in lab over the next few weeks.


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.13<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Trematoda = flukes<br />

‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />

‣ Record the descriptive information requested at the end of the lab for this species.<br />

‣ Opisthorchis (Clonorchis ) sinensis = human liver fluke<br />

‣ Observations of Trematode Body Structures<br />

‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />

trematodes.<br />

‣ Draw <strong>and</strong> CLEARLY label the Opisthorchis (Clonorchis) sinensis. whole mount.<br />

‣ oral sucker<br />

‣ ventral sucker<br />

‣ pharynx & intestines<br />

‣ excretory canals/ducts<br />

‣ uterus<br />

‣ ovary (as visible)<br />

‣ yolk gl<strong>and</strong> (as visible)<br />

‣ anterior & posterior testes<br />

‣ vas deferens (as visible)<br />

‣ seminal receptacle (as visible)<br />

‣ bladder<br />

‣ Why do you think this species lacks a complete gut (it has only one opening, not really<br />

an anus)?<br />

‣ Then why is a gut/intestine present at all?


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.14<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ Observation of an Example of Trematode Copulation<br />

‣ Draw the male <strong>and</strong> female Schistosoma mansoni in copulation from a prepared slide<br />

indicating which sex is which.<br />

‣ What aspect of their reproduction is unusual with respect to other trematode species?<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Cestoda = tapeworms<br />

‣ Observe the specimens <strong>and</strong>/or diagrams of the<br />

species listed below.<br />

‣ Record the descriptive information requested at the<br />

end of the lab for this species.<br />

‣ one adult cestode tapeworm on display<br />

‣ Observation of Cestode Body Structures<br />

‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />

cestodes.<br />

‣ Examine a prepared slide of a scolex (head) from a tapeworm such as Taenia sp. or<br />

Diplidium sp. .<br />

‣ Sketch <strong>and</strong> label the scolex. Label the four suckers, <strong>and</strong> the hooks.<br />

‣ Examine a slide of a gravid proglottid of a cestode. Note the following structures:<br />

(you do not need to draw this, but you should be able to identify these in a diagram)<br />

‣ nerve cords (as visible)<br />

male reproductive structures are not easily<br />

‣ excretory canals<br />

visible in a gravid proglottid<br />

‣ vagina/genital pore<br />

‣ Note how the ova are packed into the branched uterus<br />

‣ Roughly, how many eggs are their per gravid proglottid?<br />

(are there 10s, 100s, 1000s, millions of eggs per gravid proglottid?)


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.15<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Nemertea</strong> (Rhynchocoela = ribbonworms)<br />

‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />

‣ Record the descriptive information requested at the end of the lab for this species.<br />

‣ Baseodiscus pun<strong>net</strong>i or other<br />

‣ List one anatomical structure seen in nemerteans that is not seen in other types of<br />

aceolomate or pseudocoelomate worms.<br />

<strong>Phylum</strong> Nematoda (roundworms)<br />

‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />

‣ Record the descriptive information requested at the end of the lab for this species.<br />

‣ Ascaris lumbricoides = human intestinal parasite<br />

‣ Observation of Nematode Body Structures<br />

‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />

cestodes.<br />

‣ Examine a prepared slide of Ascaris lumbricoides male <strong>and</strong> female. Note the<br />

following structures: (you do not need to draw this, but you should be able to<br />

identify these in a diagram)<br />

‣ epidermis<br />

‣ cuticle<br />

‣ muscle cells & processes<br />

‣ intestine<br />

‣ pseudocoel<br />

‣ longitudinal muscles<br />

‣ lateral lines/nerve cords<br />

‣ excretory canals (as visible)<br />

‣ In the female Ascaris cross section locate the following:<br />

‣ ovary<br />

‣ oviduct<br />

‣ uterus Can you recognize the ova?<br />

‣ In the male Ascaris cross-section (on the same slide) locate the :<br />

‣ testes<br />

‣ vas deferens (as visible)<br />

‣ Observation of a Free-Living Nematode Roundworm<br />

Obtain a sample of living “vinegar eels”, Turbatrix aceti, <strong>and</strong> examine under the 10X<br />

objective <strong>and</strong> the 20X or 40X of the compound microscope. You should add Proto-Slo<br />

to the preparation before adding the cover slip.<br />

‣ Note the direction of movement, which is actually dorso-ventral (rather than<br />

lateral) bending.<br />

‣ What is it about the muscular structural arrangement in nematodes that permits<br />

only this characteristic “whip-like” motion?


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.16<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

‣ Is the reproductive cycle of Turbatrix aceti as complex as that of Ascaris<br />

lumbricoides? Why, or why not do you think this is the case? Look this up in the text<br />

or on the Inter<strong>net</strong>.<br />

<strong>Phylum</strong> Rotifera (rotifers)<br />

‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />

‣ Record the descriptive information requested at the end of the lab for this species.<br />

‣ a rotifer (you will probably be unable to identify it to species, so just provide a<br />

common name)<br />

‣ Examine the culture of rotifers. Examine a sample on a slide with Proto-Slo under<br />

the compound microscope.<br />

‣ Draw <strong>and</strong> CLEARLY label the following rotifer structures, otherwise be able to<br />

identify the following structures:<br />

‣ corona<br />

‣ mastax (jaw apparatus)<br />

‣ trochus (ciliated whorls)<br />

‣ stomach<br />

‣ intestine<br />

‣ mouth<br />

‣ foot <strong>and</strong> toes (for anchoring)<br />

‣ cloacal bladder (as visible)<br />

‣ pedal gl<strong>and</strong>s (as visible)<br />

‣ eyespots near brain (as visible)


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.17<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

For the live specimens available for observation in the lab, record the requested<br />

information.<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Turbellaria<br />

Order Tricladida<br />

Scientific name: Dugesia sp. or other<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.18<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Turbellaria<br />

Order Polycladida<br />

Scientific name of a polyclad turbellarian:<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.19<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Trematoda<br />

Scientific name: Opisthorchis (Clinorchis) sinensis or other<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:<br />

Fluke lifecycle


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.20<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />

Class Cestoda<br />

Scientific name:<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:<br />

Pig Tapeworm Lifecycle


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.21<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> <strong>Nemertea</strong> (Rhynchocoela)<br />

Class (check ITIS):<br />

Scientific name: Baseodiscus pun<strong>net</strong>i or other<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.22<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> Nematoda<br />

Class (check ITIS):<br />

Scientific name: Ascaris lumbricoides<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.23<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> Nematoda<br />

Class (check ITIS):<br />

Scientific name:<br />

Common name: Vinegar Eels<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.24<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

<strong>Phylum</strong> Rotifera<br />

Class (check ITIS):<br />

Scientific name:<br />

Common name:<br />

General dimensions of specimen:<br />

Unique structures or features:<br />

Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />

characteristics observed.<br />

Notes & observations to help you remember <strong>and</strong> distinguish this group/species:


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.25<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

LABORATORY NOTES:<br />

Platodes by Haeckel


<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />

Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.26<br />

Lab #6 - Biological Sciences 102 – Animal Biology<br />

LABORATORY NOTES:

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