Acoelomates: Phylum Platyhelminthes and Nemertea - Biosciweb.net
Acoelomates: Phylum Platyhelminthes and Nemertea - Biosciweb.net
Acoelomates: Phylum Platyhelminthes and Nemertea - Biosciweb.net
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<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.1<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
This lab is designed to explore the basic life history, physiology <strong>and</strong> adaptations of the<br />
acoelomate <strong>and</strong> pseudocoelomate animals with a focus on the Phyla Playthelminthes, <strong>Nemertea</strong><br />
(Rhynchocoela), Nematoda, Rotifera. We will review the lifecycles of many of these species<br />
with an emphasis on their commonly parasitic nature. You must learn these lifecycles.<br />
Refer to chapters in the textbook for illustrations, diagrams, <strong>and</strong><br />
additional information about acoelomates & pseudocoelomates.<br />
The Acoelomate Animals<br />
The acoelomates are animals that have no coelom (body cavity). They include flatworms,<br />
phylum <strong>Platyhelminthes</strong> (Gr. platys, flat, + helmins, worm); ribbon worms, phylum <strong>Nemertea</strong><br />
(Gr. Nemertes, one of the Nereids, mermaids of Greek mythology); <strong>and</strong> jaw worms, phylum<br />
Gnathostomulida (Gr. gnathos, jaw, + stoma, mouth). In acoelomate animals, the space<br />
between the body wall (ectoderm) <strong>and</strong> digestive tract (endoderm) is not a cavity, as in<br />
coelomate animals, but is filled with muscle fibers <strong>and</strong> a loose tissue of mesenchymal<br />
origin, called parenchyma, both derived from mesoderm. The presence of a well-developed<br />
mesodermal layer makes the acoelomates triploblastic (having three germ layers: ectoderm,<br />
endoderm, <strong>and</strong> mesoderm). Flatworms are a large <strong>and</strong> economically important group because<br />
they include not only free-living planarians but also parasitic tapeworms <strong>and</strong> flukes.<br />
<strong>Acoelomates</strong> are more complex in organization than the radiate animals in several ways:<br />
‣ <strong>Acoelomates</strong> have bilateral symmetry<br />
‣ They have defined tissues organized into functional organs<br />
‣ They have a highly organized nervous system with concentration of nervous tissue <strong>and</strong><br />
sense organs in the anterior end (cephalization)<br />
‣ They have an excretory system of specialized flame cells <strong>and</strong> tubules for elimination of<br />
nitrogenous wastes (breakdown products of protein, nucleic acid <strong>and</strong> other metabolism)<br />
‣ <strong>Platyhelminthes</strong> have a gastrovascular system, but ribbon worms have separated the<br />
two functions <strong>and</strong> have a complete mouth-to-anus digestive tract <strong>and</strong> a circulatory system<br />
‣ Flatworms have a tissue-organ level of organization<br />
Body Plan Features Retained by <strong>Acoelomates</strong> (seen in previously studied phyla):<br />
‣ true tissues present<br />
Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
(not seen in the <strong>Phylum</strong> Cnidaria – some of these traits are seen in other animal phyla in<br />
addition to the <strong>Phylum</strong> <strong>Platyhelminthes</strong>):<br />
1. acoelomate<br />
2. bilateral symmetry<br />
3. triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />
4. some degree of cephalization = head with sensory organs<br />
5. more organ systems (simple <strong>and</strong> formed of true tissues) are present<br />
6. flame cells in flame bulbs for excretion<br />
7. gastrovascular cavity for digestion <strong>and</strong> gas exchange (organs near body surface)<br />
8. longitudinal, circular <strong>and</strong> ventro-dorsal muscles<br />
9. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />
10. high surface-volume ratio as a result of flat shape (Important in locomotion <strong>and</strong> in<br />
exchange of nutrients <strong>and</strong> wastes).<br />
11. monoecious (hermaphroditic) = male <strong>and</strong> female organs in one individual<br />
12. most are parasitic, but some are free-living
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.2<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
Classification & Adaptations of Acoelomate Animals<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong> (flatworms)<br />
Class Turbellaria (tur'bel-lar' e-a) (L. turbellae, stir, bustle, + aria, like or connected with).<br />
About 3000 species; Turbellarians. Mostly free-living, with a ciliated epidermis. A paraphyletic<br />
grouping. Example: Dugesia tigrina<br />
Class Monogenea (mon'o-gen'e-a) (Gr. mono, single, + gene, origin, birth). About 1100 species;<br />
Monoge<strong>net</strong>ic flukes. Adult body covered with syncytial tegument without cilia; leaflike to<br />
cylindrical in shape; posterior attachment organ with hooks, suckers, or clamps, usually in<br />
combination; all parasitic, mostly on skin or gills of fishes; single host; monoecious; usually<br />
free-swimming ciliated larva. Examples: Polystoma, Gyrodactylus<br />
Class Trematoda (trem'a-to'da) (Gr. trematodes, with holes, + eidos, form). About 10,000<br />
species; Dige<strong>net</strong>ic flukes. Adult body covered with nonciliated syncytial tegument; leaf-like or<br />
cylindrical in shape; usually with oral <strong>and</strong> ventral suckers, no hooks; development indirect,<br />
first host a mollusc, final host usually a vertebrate; parasitic in all classes of vertebrates.<br />
Examples: Fasciola, Clonorchis, Schistosoma<br />
Class Cestoda (ses-to'da) (Gr. kestos, girdle, + eidos, form). About 3500 species; Tapeworms.<br />
Adult body covered with non-ciliated, syncytial tegument; scolex with suckers or hooks,<br />
sometimes both, for attachment; long, ribbonlike body, usually divided into series of<br />
proglottids; no digestive organs; parasitic in digestive tract of all classes of vertebrates; first<br />
host may be invertebrate or vertebrate. Examples: Taenia, Diphyllobothrium<br />
<strong>Phylum</strong> <strong>Nemertea</strong> or Rhynchocoela (ribbonworms)<br />
About 600 species. Ribbon worms are often called nemertine or nemertean worms. Nearly all<br />
are marine <strong>and</strong> are characterized by an eversible proboscis that can be thrown out with great<br />
speed to capture food. Ribbon worms have separated the two functions of the gastrovascular<br />
cavity in flatworms <strong>and</strong> have a complete mouth-to-anus digestive tract <strong>and</strong> a circulatory<br />
system. <strong>Nemertea</strong>ns occur in the intertidal, under stones or sea weed, or in burrows in muddy<br />
s<strong>and</strong>. <strong>Nemertea</strong>ns can be very long at up to 50 meters (these species are the worlds<br />
longest animals).<br />
Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> <strong>Nemertea</strong><br />
(some of these traits are seen in other animal phyla in addition to the <strong>Phylum</strong> <strong>Nemertea</strong>):<br />
1. acoelomate, bilateral symmetry, triploblastic structure<br />
2. some degree of cephalization = head with sensory organs<br />
3. some organ systems (formed of true tissues) are present<br />
4. eversible proboscis (only worm phylum with this characteristic)<br />
5. complete digestive tract/system (from mouth-to-anus)<br />
6. separate circulatory system with two lateral vessels on each side of the gut <strong>and</strong><br />
sinuses around head <strong>and</strong> tail; no definite closed circulation<br />
7. flame cells for excretion<br />
8. longitudinal <strong>and</strong> circular muscles contract against fibrous basement membrane;<br />
multiple patterns of muscle fiber organization<br />
9. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />
10. dioecious with sexual reproduction or asexual fragmentation<br />
11. not parasitic, free-living
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.3<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
The Pseudocoelomate Animals<br />
All bilateral animal phyla except the acoelomates possess a body cavity belonging to one of<br />
two types: (1) true coelom, in which a peritoneum (an epithelium of mesodermal origin)<br />
covers both the inner surface of the body wall <strong>and</strong> the outer surface of the visceral<br />
organs in the cavity, or (2) pseudocoel, a body cavity not entirely lined with peritoneum (one<br />
layer of mesoderm attached to the ectoderm).<br />
A body cavity of either type is an advantage because it provides room for organ development<br />
<strong>and</strong> storage <strong>and</strong> allows some freedom of movement within the body. The cavity is often fluidfilled<br />
<strong>and</strong> provides for a hydrostatic skeleton in those forms lacking a true skeleton.<br />
There are nine pseudocoelomate phyla, of which phylum Nematoda is by far the largest. All<br />
pseudocoelomate phyla are at the organ-system level of organization.<br />
In general pseudocoelomates tend to be cylindrical in body form, to be unsegmented, <strong>and</strong> to<br />
have a complete (mouth-to-anus) digestive tract (this is absent in acanthocephalans). The<br />
epidermis is usually covered with a cuticle. There are both aquatic <strong>and</strong> terrestrial members,<br />
<strong>and</strong> parasitism is fairly common.<br />
Body Plan Features Retained by Pseudocoelomates (seen in previously studied phyla):<br />
1. presence of true tissues<br />
2. bilateral symmetry<br />
3. triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />
4. some degree of cephalization = head with sensory organs<br />
5. some organ systems (formed of true tissues) are present<br />
Body Plan Features & Characteristics of Pseudocoelomates:<br />
1. possess a pseudocoelom (mesoderm lines ectoderm tissue side only)<br />
2. they are unsegmented<br />
3. complete digestive tract/system (from mouth-to-anus)<br />
4. epidermis is covered with a cuticle
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.4<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
Classification & Adaptations of Pseudocoelomate Animals<br />
<strong>Phylum</strong> Nematoda (roundworms)<br />
More than 25,000 species. Roundworms. Nematodes are an extensive group with worldwide<br />
distribution. They include terrestrial, freshwater, marine, <strong>and</strong> parasitic forms. They are<br />
elongated roundworms covered with a flexible, nonliving cuticle. Circular muscles are lacking<br />
in the body wall <strong>and</strong>, in Ascaris, longitudinal muscles are arranged in four groups separated by<br />
epidermal cords (some nematodes have six or eight groups of longitudinal muscles). Cilia are<br />
completely lacking. Nematodes are found free-living in almost every conceivable habitat from<br />
arid deserts to lake bottoms, rivers, polar seas. Nematodes - both parasitic <strong>and</strong> freeliving - are<br />
incredibly abundant. A h<strong>and</strong>ful of good garden soil contains thous<strong>and</strong>s of nematodes. Some<br />
50 different species of nematodes occur in humans, most of them nonpathogenic. Some<br />
nematodes are plant parasites feeding on plant sap, especially roots.<br />
Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> Nematoda<br />
(some of these traits are seen in other animal phyla):<br />
1. pseudocoelomate<br />
2. bilateral symmetry<br />
3. triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />
4. psuedocoelomate = possess a pseudocoelom<br />
5. some degree of cephalization = head with sensory organs<br />
6. some organ systems (formed of true tissues) are present<br />
7. vermiform (worm-like) body shape<br />
8. complete digestive tract with three angled (longitudinal, circular <strong>and</strong> oblique)<br />
muscular pharynx, intestine lacking muscular walls, short rectum <strong>and</strong> anus<br />
9. excretion <strong>and</strong> osmoregulation through the cuticle <strong>and</strong> by excretory canals<br />
10. lack both cilia <strong>and</strong> flame cells/bulbs<br />
11. body wall lined with cuticle which retains body shape<br />
12. longitudinal muscles only, no circular muscles in body wall; body length or<br />
diameter does not change in movement<br />
13. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />
14. dioecious; sexes separate with females larger than males; one or two tubular gonads;<br />
copulatory spicules present in males<br />
<strong>Phylum</strong> Rotifera (rotifers)<br />
There are about 2000 species of rotifers. The name "rotifer" is derived from the Latin word<br />
meaning "wheel-bearer"; making reference to the crown of cilia around the mouth of the rotifer.<br />
The rapid movement of the cilia in some species makes them appear to whirl like a wheel.<br />
Rotifers can be found in many freshwater environments <strong>and</strong> in moist soil. The habitat of<br />
rotifers may include still water environments, such as lake bottoms, as well as flowing water<br />
environments, such as rivers or streams. Rotifers are also commonly found on mosses <strong>and</strong><br />
lichens growing on tree trunks <strong>and</strong> rocks, in rain gutters <strong>and</strong> puddles, in soil or leaf litter, on<br />
mushrooms growing near dead trees, in tanks of sewage treatment plants, <strong>and</strong> even on<br />
freshwater crustaceans <strong>and</strong> aquatic insect larvae. Most species of rotifers are about 200 to 500<br />
micrometers long. However a few species, such as Rotaria neptunia may be longer than a<br />
millimeter. Rotifers are thus multicellular creatures who make their living at the scale of<br />
unicellular protists. Characteristic features of rotifers include the ciliated corona ("wheel<br />
organ") <strong>and</strong> the mastax. The corona is located anteriorly <strong>and</strong> functions in locomotion <strong>and</strong> food<br />
gathering. The corona is modified extensively in some species. The mastax is a muscular<br />
pharynx containing a complex set of hard jaws or trophi <strong>and</strong> is found in all rotifers. These<br />
characteristics of these structures have been used extensively in classifying rotifers.
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.5<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
Body Plan Features & Characteristics of Members of the <strong>Phylum</strong> Rotifera<br />
(some of these traits are seen in other animal phyla):<br />
1. bilateral symmetry <strong>and</strong> triploblastic structure (endoderm, mesoderm <strong>and</strong> ectoderm)<br />
2. psuedocoelomate = possess a pseudocoelom<br />
3. some degree of cephalization = head with sensory organs<br />
4. some organ systems (formed of true tissues) are present<br />
5. complete digestive system with mouth <strong>and</strong> anus<br />
6. well muscularized pharynx = mastax that contains trophi (jaws) for sucking <strong>and</strong><br />
grinding food particles<br />
7. ciliated corona helps sweep food particles to mouth<br />
8. pair of protonephridial tubules for osmoregulation & excretion<br />
9. syncitial (multinucleate) epidermis; some have a secreted cuticle<br />
10. longitudinal muscles <strong>and</strong> circular muscles in body wall<br />
11. pedal gl<strong>and</strong>s of foot secrete adhesive material in both sessile <strong>and</strong> creeping forms<br />
12. hydrostatic skeleton (as muscles push against parenchyma <strong>and</strong> tissue fluids)<br />
13. dioecious; sexes separate with females larger than males; one or two tubular gonads;<br />
copulatory spicules present in males; can be parthenogenic<br />
‣ Reproduction in Rotifers<br />
‣ single (male) or double set of gonads (female) <strong>and</strong> ducts in each sex<br />
‣ sexes separate (dioecious)<br />
‣ males unknown in class Bdelloidea <strong>and</strong> only occur a few weeks a year in the class<br />
Monogononta<br />
‣ all females are parthenoge<strong>net</strong>ic in the class Bdelloidea (diploid females produce<br />
diploid females)<br />
‣ parthenogenesis = unisexual reproduction involving the production of young by<br />
females that are not fertilized by males; common in rotifers, aphids, bees, ants <strong>and</strong><br />
wasps. Parthenoge<strong>net</strong>ic eggs may be haploid or diploid.<br />
‣ in the class Monogononta, most of the year, diploid females produce thin shelled<br />
diploid amictic eggs. These amictic eggs develop parthenoge<strong>net</strong>ically into diploid<br />
(amictic) females. When the environment in the pond or stream changes due to<br />
crowding, diet, change in photoperiod, etc. some amictic eggs develop into diploid<br />
mictic females that produce thin shelled haploid eggs. If these eggs are not fertilized<br />
then they will develop into haploid males. If the eggs are fertilized, they become<br />
mictic eggs with a thick, resistant shell <strong>and</strong> become dormant. These “winter eggs”<br />
can survive until the environment becomes more suitable at which time they hatch <strong>and</strong><br />
develop into amictic females (completing the cycle). Females grow <strong>and</strong> reach maturity<br />
in a few days while males rarely grow <strong>and</strong> are mature at birth.
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.6<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ Concepts Related to Parasitic Worms<br />
Basic Requirements for a Parasitic Life<br />
1. The parasite must have or obtain access to a host(s)<br />
2. The parasite must establish itself <strong>and</strong> survive within the host(s)<br />
3. The parasite must show successful reproduction <strong>and</strong> transmission to a new host<br />
Special Parasitic Adaptations<br />
1. Invasion of the host can occur through many mechanisms:<br />
a. through the mouth with food <strong>and</strong>/or water<br />
b. through the skin by pe<strong>net</strong>ration<br />
c. through a vector such as a biting insect<br />
2. Establishment <strong>and</strong> survival within a host requires:<br />
a. transport of parasites/larvae/eggs in host through blood or lymph<br />
b. resistance to host's defenses (such as resistance to immune cells, circulating<br />
antibodies <strong>and</strong> acids <strong>and</strong> enzymes in the digestive tract for intestinal parasites)<br />
3. Reproduction, by cross fertilization:<br />
a. most trematodes <strong>and</strong> cestodes are monoecious (both sexes in one individual)<br />
b. most nematodes are dioecious; generally, males are smaller than females<br />
4. Parasitic Worm Life Cycles<br />
SEE THE DETAILED HANDOUT AVAILABLE ON THE COURSE WEBSITE<br />
a. Fertilization occurs within a host <strong>and</strong> fertilization is internal as a result of<br />
copulation. The parasite zygote develops into a shelled embryo which develops<br />
into a larval stage(s).<br />
b. SIMPLE PATTERN (monogenes, some tapeworms, nematodes)<br />
ZYGOTE -----> LARVA -----> ADULT -----> MANY EGGS<br />
c. COMPLEX PATTERN (digenes <strong>and</strong> some tapeworms)<br />
ZYGOTE --> 1st LARVAL STAGE--> 2nd LARVAL STAGE--><br />
3rd LARVAL STAGE --> ADULT --> MANY EGGS<br />
5. Dige<strong>net</strong>ic trematodes <strong>and</strong> cestodes have specialized surfaces consisting of a<br />
cellular syncytium with microvilli (microtrichs) which serve to increase the<br />
absorptive surface up to 50 times. Membrane surface serves to protect against<br />
host's digestive enzymes, serves in chemical modification of absorbed<br />
solutes, <strong>and</strong> serves in membrane transport
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.7<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ LIFECYCLES OF HELMINTHS (Worms)<br />
‣ TREMATODES (Class Trematoda)<br />
Clonorchis sinensis = The oriental liver fluke<br />
HOST (human)<br />
adult worm in liver<br />
shelled embryo<br />
miracidium<br />
SNAIL<br />
FISH<br />
metacercaria<br />
(in muscle)<br />
cercaria<br />
redia<br />
sporocyst<br />
Schistosoma mansoni -- Blood fluke<br />
HOST (human)<br />
adult worm in<br />
liver<br />
shelled embryo<br />
miracidium<br />
cercaria<br />
sporocyst<br />
SNAIL<br />
‣ CESTODES (Class Cestoda)<br />
Taeniarhynchus saginatus - The beef tapeworm<br />
ADULT HOST (humans)<br />
adult worm in intestine<br />
shelled embryos<br />
in proglottid<br />
consumed with<br />
grass by cattle<br />
cysticercus (bladder worm)<br />
in beef muscle<br />
onchospheres<br />
in blood
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.8<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ NEMATODES (<strong>Phylum</strong> Nematoda)<br />
Ascaris lubricoides = intestinal roundworm<br />
Ascaris lumbricoides -- Intestinal roundworm<br />
Adults (male & female)<br />
in human intestine<br />
shelled embryos<br />
in feces<br />
shelled larvae<br />
develop (10-15<br />
days) on soil.<br />
in lung, larvae leave<br />
blood, move to<br />
trachea, esophagus<br />
larvae emerge in<br />
duodenum, pe<strong>net</strong>rate gut<br />
wall <strong>and</strong> enter blood stream<br />
shelled larvae<br />
ingested by humans<br />
with food or water<br />
Ancylostoma duodenale = hookworm<br />
Ancylostoma duodenale -- Hookworm<br />
Adults in human<br />
intestine<br />
thin shelled embryos in feces<br />
larvae develop,<br />
hatch in soil<br />
esophagus<br />
larvae grow,<br />
feed on feces<br />
bronchi<br />
in lung, larvae leave<br />
blood, enter alveoli<br />
larvae attach,<br />
pe<strong>net</strong>rate skin,<br />
enter blood stream<br />
Enterobius vermicularis = pinworm<br />
Enterobius vermicularis -- Pinworm<br />
Adults (male & female)<br />
in human large intestine<br />
<strong>and</strong> cecum<br />
females migrate to<br />
anus at night<br />
deposited shelled<br />
embryos cause itching<br />
hatch in duodemum<br />
reinfection of self,<br />
others, water <strong>and</strong> food.<br />
Also in air or dust.<br />
fingernails <strong>and</strong><br />
clothing pick up<br />
shelled embryos
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.9<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
LAB PROCEDURE<br />
NAME:<br />
LAB SCORE:<br />
Refer to the textbook <strong>and</strong> the inter<strong>net</strong> to help you answer the questions.<br />
You must answer ALL questions in the lab procedure for full credit.<br />
Finish them at home if you do not have time to complete them in lab.<br />
Answer these questions:<br />
List some advantages of bilateral symmetry over radial symmetry?<br />
What advantages does cephalization offer for a bilateral animal?<br />
<strong>Acoelomates</strong> <strong>and</strong> pseudocoelomates have excretory systems of specialized flame cells or<br />
protonephridia for the elimination of nitrogenous wastes. How does a radiate animal, such as<br />
a hydra or sea anemone, rid itself of waste molecules?<br />
Cilia are completely lacking in nematodes. Are cilia present in any acoelomates? If so,<br />
during what life stage are the cilia often obvious?<br />
Are cilia present in any cnidarians?
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.10<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Turbellaria = free-living flatworms<br />
‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />
‣ Record the descriptive information requested at the end of the lab for each species.<br />
‣ Dugesia sp., a triclad flatworm<br />
‣ an available polyclad flatworm<br />
‣ What are the two major orders of flatworms in the Class Turbellaria?<br />
‣ Observations of a Free-Living Planarian Flatworm<br />
‣ Obtain a living specimen of Dugesia tigrina or Planaria sp. as available in a small<br />
petri dish or watch glass <strong>and</strong> study it under the dissecting microscope.<br />
Species name of your observed specimen:<br />
‣ Note the gliding movement. What causes it?<br />
‣ Using forceps, gently turn your flatworm over. Observe <strong>and</strong> describe the righting response.<br />
‣ How long does the righting response take?<br />
‣ Does the worm avoid obstacles such as a needle or probe without touching them?<br />
‣ Locate the eyespots <strong>and</strong> the “ears” or auricular organs. These organs contain<br />
chemoreceptors for detection of specific molecules (eg. “smell”/olfaction). Place a small<br />
piece of liver in the dish <strong>and</strong> observe <strong>and</strong> record how the worm responds.
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.11<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ Do you think the response to the liver depends on the presence of the auricular organs?<br />
‣ Briefly describe how you could test your hypothesis?<br />
‣ Does the worm respond to differences in light intensity? Record your observations below.<br />
‣ Record the reaction of the planarian to the addition of one drop of a 0.1% salt solution<br />
placed in front of it.<br />
‣ How do the two major types of turbellarian (Class Turbellaria) flatworms differ? (eg. How do<br />
members of the Order Tricladida (triclad flatworms) differ from members of the Order<br />
Polycladida (polyclad flatworms – how are their bodies different)?
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.12<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ Observations of Planarian Flatworm Body Structures<br />
‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />
Dugesia sp.or Planaria sp.<br />
‣ Draw <strong>and</strong> CLEARLY label the Dugesia sp.or Planaria sp. whole mount.<br />
‣ eyespots<br />
‣ intestine<br />
‣ auricular organs<br />
‣ pharynx<br />
‣ Draw <strong>and</strong> CLEARLY label the Dugesia sp.or Planaria sp. cross section through the<br />
pharynx.<br />
‣ intestine<br />
‣ diverticula<br />
‣ epidermis<br />
‣ pharynx<br />
‣ cilia<br />
‣ nerve cords<br />
‣ circular muscles<br />
‣ longitudinal muscles<br />
‣ Planaria Regeneration Experiments<br />
‣ Your instructor may perform incisions on some planarian flatworms to demonstrate<br />
regeneration. We will culture <strong>and</strong> observe these specimens in lab over the next few weeks.
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.13<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Trematoda = flukes<br />
‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />
‣ Record the descriptive information requested at the end of the lab for this species.<br />
‣ Opisthorchis (Clonorchis ) sinensis = human liver fluke<br />
‣ Observations of Trematode Body Structures<br />
‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />
trematodes.<br />
‣ Draw <strong>and</strong> CLEARLY label the Opisthorchis (Clonorchis) sinensis. whole mount.<br />
‣ oral sucker<br />
‣ ventral sucker<br />
‣ pharynx & intestines<br />
‣ excretory canals/ducts<br />
‣ uterus<br />
‣ ovary (as visible)<br />
‣ yolk gl<strong>and</strong> (as visible)<br />
‣ anterior & posterior testes<br />
‣ vas deferens (as visible)<br />
‣ seminal receptacle (as visible)<br />
‣ bladder<br />
‣ Why do you think this species lacks a complete gut (it has only one opening, not really<br />
an anus)?<br />
‣ Then why is a gut/intestine present at all?
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.14<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ Observation of an Example of Trematode Copulation<br />
‣ Draw the male <strong>and</strong> female Schistosoma mansoni in copulation from a prepared slide<br />
indicating which sex is which.<br />
‣ What aspect of their reproduction is unusual with respect to other trematode species?<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Cestoda = tapeworms<br />
‣ Observe the specimens <strong>and</strong>/or diagrams of the<br />
species listed below.<br />
‣ Record the descriptive information requested at the<br />
end of the lab for this species.<br />
‣ one adult cestode tapeworm on display<br />
‣ Observation of Cestode Body Structures<br />
‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />
cestodes.<br />
‣ Examine a prepared slide of a scolex (head) from a tapeworm such as Taenia sp. or<br />
Diplidium sp. .<br />
‣ Sketch <strong>and</strong> label the scolex. Label the four suckers, <strong>and</strong> the hooks.<br />
‣ Examine a slide of a gravid proglottid of a cestode. Note the following structures:<br />
(you do not need to draw this, but you should be able to identify these in a diagram)<br />
‣ nerve cords (as visible)<br />
male reproductive structures are not easily<br />
‣ excretory canals<br />
visible in a gravid proglottid<br />
‣ vagina/genital pore<br />
‣ Note how the ova are packed into the branched uterus<br />
‣ Roughly, how many eggs are their per gravid proglottid?<br />
(are there 10s, 100s, 1000s, millions of eggs per gravid proglottid?)
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.15<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Nemertea</strong> (Rhynchocoela = ribbonworms)<br />
‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />
‣ Record the descriptive information requested at the end of the lab for this species.<br />
‣ Baseodiscus pun<strong>net</strong>i or other<br />
‣ List one anatomical structure seen in nemerteans that is not seen in other types of<br />
aceolomate or pseudocoelomate worms.<br />
<strong>Phylum</strong> Nematoda (roundworms)<br />
‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />
‣ Record the descriptive information requested at the end of the lab for this species.<br />
‣ Ascaris lumbricoides = human intestinal parasite<br />
‣ Observation of Nematode Body Structures<br />
‣ Using a microscope <strong>and</strong> the preserved, prepared slides identify the following structures of<br />
cestodes.<br />
‣ Examine a prepared slide of Ascaris lumbricoides male <strong>and</strong> female. Note the<br />
following structures: (you do not need to draw this, but you should be able to<br />
identify these in a diagram)<br />
‣ epidermis<br />
‣ cuticle<br />
‣ muscle cells & processes<br />
‣ intestine<br />
‣ pseudocoel<br />
‣ longitudinal muscles<br />
‣ lateral lines/nerve cords<br />
‣ excretory canals (as visible)<br />
‣ In the female Ascaris cross section locate the following:<br />
‣ ovary<br />
‣ oviduct<br />
‣ uterus Can you recognize the ova?<br />
‣ In the male Ascaris cross-section (on the same slide) locate the :<br />
‣ testes<br />
‣ vas deferens (as visible)<br />
‣ Observation of a Free-Living Nematode Roundworm<br />
Obtain a sample of living “vinegar eels”, Turbatrix aceti, <strong>and</strong> examine under the 10X<br />
objective <strong>and</strong> the 20X or 40X of the compound microscope. You should add Proto-Slo<br />
to the preparation before adding the cover slip.<br />
‣ Note the direction of movement, which is actually dorso-ventral (rather than<br />
lateral) bending.<br />
‣ What is it about the muscular structural arrangement in nematodes that permits<br />
only this characteristic “whip-like” motion?
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.16<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
‣ Is the reproductive cycle of Turbatrix aceti as complex as that of Ascaris<br />
lumbricoides? Why, or why not do you think this is the case? Look this up in the text<br />
or on the Inter<strong>net</strong>.<br />
<strong>Phylum</strong> Rotifera (rotifers)<br />
‣ Observe the specimens <strong>and</strong>/or diagrams of the species listed below.<br />
‣ Record the descriptive information requested at the end of the lab for this species.<br />
‣ a rotifer (you will probably be unable to identify it to species, so just provide a<br />
common name)<br />
‣ Examine the culture of rotifers. Examine a sample on a slide with Proto-Slo under<br />
the compound microscope.<br />
‣ Draw <strong>and</strong> CLEARLY label the following rotifer structures, otherwise be able to<br />
identify the following structures:<br />
‣ corona<br />
‣ mastax (jaw apparatus)<br />
‣ trochus (ciliated whorls)<br />
‣ stomach<br />
‣ intestine<br />
‣ mouth<br />
‣ foot <strong>and</strong> toes (for anchoring)<br />
‣ cloacal bladder (as visible)<br />
‣ pedal gl<strong>and</strong>s (as visible)<br />
‣ eyespots near brain (as visible)
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.17<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
For the live specimens available for observation in the lab, record the requested<br />
information.<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Turbellaria<br />
Order Tricladida<br />
Scientific name: Dugesia sp. or other<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.18<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Turbellaria<br />
Order Polycladida<br />
Scientific name of a polyclad turbellarian:<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.19<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Trematoda<br />
Scientific name: Opisthorchis (Clinorchis) sinensis or other<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:<br />
Fluke lifecycle
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.20<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Platyhelminthes</strong><br />
Class Cestoda<br />
Scientific name:<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:<br />
Pig Tapeworm Lifecycle
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.21<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> <strong>Nemertea</strong> (Rhynchocoela)<br />
Class (check ITIS):<br />
Scientific name: Baseodiscus pun<strong>net</strong>i or other<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.22<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> Nematoda<br />
Class (check ITIS):<br />
Scientific name: Ascaris lumbricoides<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.23<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> Nematoda<br />
Class (check ITIS):<br />
Scientific name:<br />
Common name: Vinegar Eels<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.24<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
<strong>Phylum</strong> Rotifera<br />
Class (check ITIS):<br />
Scientific name:<br />
Common name:<br />
General dimensions of specimen:<br />
Unique structures or features:<br />
Draw a simple sketch to remind you of the basic structure of this species <strong>and</strong> any unique<br />
characteristics observed.<br />
Notes & observations to help you remember <strong>and</strong> distinguish this group/species:
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.25<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
LABORATORY NOTES:<br />
Platodes by Haeckel
<strong>Acoelomates</strong>: <strong>Phylum</strong> <strong>Platyhelminthes</strong> <strong>and</strong> <strong>Nemertea</strong> <strong>and</strong><br />
Pseudocoelomates: Phyla Nematoda <strong>and</strong> Rotifera & Parasitism 6.26<br />
Lab #6 - Biological Sciences 102 – Animal Biology<br />
LABORATORY NOTES: