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Ionix Pharmaceuticals - Molecular Devices

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Adherent vs Suspension Assay<br />

Cells used in suspension<br />

IMR32 cells adhere to surfaces weakly<br />

Changing media every 2-3 days causes<br />

large loss of cells when grown in 96 well<br />

plates<br />

Cells grown in tissue culture flasks<br />

(under differentiating conditions, if<br />

necessary) and then loaded with<br />

fluorescent indicator and assayed in<br />

suspension<br />

8

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