Ionix Pharmaceuticals - Molecular Devices

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Aim To develop a medium throughput fluorescence-based assay using IMR32 cells in 96-well plate format 6
IMR32 Cell Culture Conditions Cells grown in EMEM supplemented with 2mM Glutamine+1%NEAA+10%FBS Cells were split 1:2/1:3 every 2-3 days or had the media changed Differentiate with 1mM dibutyryl cAMP and 2.5µM bromodeoxyuridine for 8-10 days 7
Page 1 and 2: Using the FLEXstation to investigat
Page 3 and 4: Ionix Profile Founded July 2001, C
Page 5: Voltage-gated Calcium Channels IMR
Page 9 and 10: Fura-2 Fluorescent Indicator 340/3
Page 11 and 12: KCl-Induced Calcium Changes In IMR-
Page 13 and 14: 1M Nicardipine +1M -CnTxGVIA 13 Pha
Page 15 and 16: Increasing CaCl 2 Concentration in
Page 17 and 18: Calcium Channel Screen 50,000 comp
Page 19 and 20: 19 SOFTmax PRO Data Analysis
Page 21 and 22: Summary Developed assay to analyze
Page 23 and 24: Experimental Conditions Neuroblast
Page 25 and 26: Assay Validation Responses measure
Page 27 and 28: Summary of Data Cell Line SH-SY-5Y
Page 29 and 30: Summary Tetrodotoxin sensitive vol
Page 31: Acknowledgements Molecular Pharmac

Ionix Pharmaceuticals - Molecular Devices

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