MP491/6-Undergraduate Research Project Proposal 1. Title ... - UCSI

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Due to the overlap of their absorption and emission spectra and matrix effects found when working with chemical/biological samples (emission or absorption of the matrix components, attenuation of analyte signals, etc.), the determination of levofloxacin, by means of spectrophotometric or spectrofluorometric methods, traditionally requires a prior separation stage. This step is usually performed by HPLC techniques. Considering the rapid nature and economical advantages of HPTLC method of analysis when compared to HPLC method of analysis, development of HPTLC method for analysis of levofloxacin in different dosage forms is proposed through this project proposal. Also, the literature review indicated that only one HPTLC method has been reported so far for the analysis of levofloxacin. However, the detailed introspection of the reported method revealed that lamotrigine was used as an internal standard. It is proposed to use levofloxacin as an internal standard in our method. Also the mobile phase used was water-methanol-n-butanol-ammonia solution. In our previous personal study, it was found that this mobile phase took long time to ascend the TLC plate resulting in unusually longer duration of analysis. So, it is proposed to use a better mobile phase in our method to shorten the analysis time. The methodology to be used for development of HPTLC analysis method in this project is described as follows. A suitable solvent would be identified for dissolving the different dosage forms so as to get maximum amount of levofloxacin in solution (this would again be confirmed with recovery study also). Then a suitable mobile phase would be selected based on the chemistry of levofloxacin and trial and error method. Then the UV densitometric analysis by absorbance mode would be carried out to estimate the amount of levofloxacin in different dosage forms. The objective of this study is to develop an improved HPTLC method which can be used for routine analysis of levofloxacin dosage forms in a pharmaceutical quality control laboratory. 9. Title: Determination of Heavy Metals in Herbal Supplements in Malaysia Market Supervisor(s) Main: Ms. Wong Pooi See Co- : Dr. Gabriel Akowuah Objective(s) 1. To identify type of heavy metals in herbal supplements in Malaysia market 2. To determine the concentration/level of heavy metals in herbal supplements in Malaysia market. Abstract Herbal supplements become more popular in Malaysia for the past 20 years. The Drug Control Authority (DCA) in Malaysia have recognized the increasing use of herbal medicines and implemented the phase 3 registration of traditional medicines. The registration criteria for any traditional medicine in Malaysia include limits for heavy metals such as lead, mercury, arsenic and cadmium. Most of the supplements are sold as OTC products in the local market. Some of the products might not have gone through 6
the appropriate registration procedure. High level of heavy metals in supplements will lead to severe adverse effects to the human body such as kidney failure and neurological damage. Four metals (Cd, Pd, Hg and As) will be determined from the herbal supplement products by using Atomic atomic absorption spectroscopy (AAS) and inductively coupled plasma-atomic emission spectrometry (ICP-AES). The obtained result will be assessed in compliance with the WHO Standard. 10. Title: Estimation of antioxidant activity of lawsone by using potentiometer Supervisor(s) Main: Mr.Bontha V S Lokesh Co- : None Objective(s) 1. To design a methodology for estimation of the antioxidant activity of pure lawsone and its content in henna leaves electrochemically 2. Comparison of the antioxidant effect of lawsone with powerful antioxidant vitamins like Vitamin C and E 3. Estimation of purity of commercial herbal henna products in Malaysia. Abstract Lawsonia inermis (henna) contains a red-orange pigment, (lawsone), which is also known as hennotannic acid. When henna leaves are crushed in an acidic medium and applied to skin, the lawsone molecules migrate from the henna paste, traverse the outermost skin layer stratum corneum and stain the layer. It is powerful antioxidant. Lawsone is chemically known as 2-hydroxy-1,4-naphthoquinone and is also described as Natural Orange 6 industrially. The name and molecular structure of lawsone show its congeniality to naphthalene 2 . In lawsone, two oxygen atoms are attached to the naphthalene carbons at positions 1 and 4 to form 1,4-naphoquinone and a hydroxyl (–OH) group is present at position 2. Its molecule formula is given to be C 10 H 6 O 3 and its total molecular weight of 174.16. Pure lawsone is an orange powder, 7
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MP491/6-Undergraduate Research Project Proposal 1. Title ... - UCSI

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