MP491/6-Undergraduate Research Project Proposal 1. Title ... - UCSI

Due to the overlap of their absorption and emission spectra and matrix effects
found when working with chemical/biological samples (emission or absorption of the
matrix components, attenuation of analyte signals, etc.), the determination of
levofloxacin, by means of spectrophotometric or spectrofluorometric methods,
traditionally requires a prior separation stage. This step is usually performed by HPLC
techniques.
Considering the rapid nature and economical advantages of HPTLC method of
analysis when compared to HPLC method of analysis, development of HPTLC method
for analysis of levofloxacin in different dosage forms is proposed through this project
proposal.
Also, the literature review indicated that only one HPTLC method has been
reported so far for the analysis of levofloxacin. However, the detailed introspection of the
reported method revealed that lamotrigine was used as an internal standard. It is
proposed to use levofloxacin as an internal standard in our method. Also the mobile
phase used was water-methanol-n-butanol-ammonia solution. In our previous personal
study, it was found that this mobile phase took long time to ascend the TLC plate
resulting in unusually longer duration of analysis. So, it is proposed to use a better
mobile phase in our method to shorten the analysis time.
The methodology to be used for development of HPTLC analysis method in this
project is described as follows. A suitable solvent would be identified for dissolving the
different dosage forms so as to get maximum amount of levofloxacin in solution (this
would again be confirmed with recovery study also). Then a suitable mobile phase would
be selected based on the chemistry of levofloxacin and trial and error method. Then the
UV densitometric analysis by absorbance mode would be carried out to estimate the
amount of levofloxacin in different dosage forms.
The objective of this study is to develop an improved HPTLC method which can
be used for routine analysis of levofloxacin dosage forms in a pharmaceutical quality
control laboratory.
9. Title: Determination of Heavy Metals in Herbal Supplements in Malaysia Market
Supervisor(s)
Main: Ms. Wong Pooi See
Co- : Dr. Gabriel Akowuah
Objective(s)
1. To identify type of heavy metals in herbal supplements in Malaysia market
2. To determine the concentration/level of heavy metals in herbal supplements in
Malaysia market.
Abstract
Herbal supplements become more popular in Malaysia for the past 20 years. The Drug
Control Authority (DCA) in Malaysia have recognized the increasing use of herbal
medicines and implemented the phase 3 registration of traditional medicines. The
registration criteria for any traditional medicine in Malaysia include limits for heavy
metals such as lead, mercury, arsenic and cadmium. Most of the supplements are sold
as OTC products in the local market. Some of the products might not have gone through
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