MP491/6-Undergraduate Research Project Proposal 1. Title ... - UCSI

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2. Title: Antimicrobial Susceptibility Testing of Murraya koenigii extract on Staphylococcus aureus Supervisor(s) Main: Ms Priya Co- : Dr. Thet Thet Htar Objective(s) 1. To determine the minimal inhibitory concentration value using disk diffusion method and microdilution assay on Staphylococcus aureus cultures. Abstract Natural products have shown that they are potential source of antimicrobial agents, such as neem leaves and curry leaves. Since antimicrobial therapy is going through a crisis with the increasing resistance towards antimicrobial agents, it is important to screen new plants and study their effectiveness in their antimicrobial activities. Staphylococcus aureus, gram positive bacteria that typically occur in grape-like clusters have yellow pigmented colonies. They seem to be the most pathogenic of all the staphylococci and are often a problem in the hospital environment. Infections by staphylococci have become difficult to treat as they have become resistant to many antibiotics that are exposed in the hospital environment. Folliculitis, food poisoning, toxic shock syndrome, impetigo are some of the diseases caused by S. aureus strains. 3. Title: Sub-chronic (28 days) toxicity study of Limonia acidissima bark extract in rats. Supervisor(s) Main: Dr. Chin Jin Han Co-: Dr. Gabriel Akowuah Objective(s) 1. To examine the possible toxic or adverse effect of L. acidissima bark extract in rats. 2. To determine LD 50 of Limonia acidissima extract. Abstract Limonia acidissima (Rutaceae) is commonly known as wood apple (locally named belinggai), is distributed in dry warm regions of Burma, India, Malaya and Sri Lanka. Oil and constituents derived from the leave, bark, roots and fruit pulp are used as topical application on venomous wounds and against snakebite. An interesting feature of the plants classified within the family Rutaceae is that they contain several types of cytotoxic, antimicrobial, neuroactive quinoleic alkaloids derived from anthranilic acid. The plant has been reported to contain limonoids in stem bark and root bark, alkaloids, benzoquinone, flavonoids, triterpenoids in root bark, stem bark, leaves and fruits. However, toxicity studies on this plant have not been reported extensively. Therefore, this study aims to ensure the safe use of this plant for therapeutic purposes. 2
4. Title: Toxicity effects of sub-chronic administration of Limonia acidissima bark extract on blood parameters of rats. Supervisor(s) Main: Prof Noran Co- : Dr Chin Objectives 1. To measure the haematocrit values of normal and treated rats. 2. To determine RBC and WBC counts. 3. To measure the Hb content of RBC. 4. To prepare blood smears of normal and treated rats using giemsa and Wright staining methods for morphological evaluation. Abstract The cellular components of blood are essential for carrying out its vital functions. The erythrocytes (RBC) are primarily involved in oxygen and carbon dioxide transport, the leucocytes (WBC) constitute an important part of the defence and immune systems of the body, and thrombocytes (platelets) are vital for blood clotting. Any changes in these blood components due to infections or exposure to chemicals will ultimately affect normal physiological functions, hence monitoring these parameters is one of the approaches in determining the effects of exposure to Limonia acidissima bark extract. 5. Title: Toxicity effects of sub-chronic administration of Limonia acidissima bark extract on sperm quality of rats. Supervisor(s) Main: Prof Noran Co- : Dr Chin Objective(s) 1.Extraction of seminal fluid from isolated rat epididymis for determination of mortality rate, sperm counts and percentage of progressive forward movements. 2.Preparation of sperm smears for morphological analyses. Abstract Sub-chronic or chronic exposure to various chemicals or drugs may affect sperm production and morphology in males. Although application of extract from the plant Limonia acidissima has been reported to have many beneficial effects but there has been no report on the effects of exposure to sub-chronic doses of its bark extract on fertility. One of the first sign would be damages to sperm production, hence the fist step would be to look at semen quality by studying sperm viability. 3
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MP491/6-Undergraduate Research Project Proposal 1. Title ... - UCSI

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