MP491/6-Undergraduate Research Project Proposal 1. Title ... - UCSI

2. Title: Antimicrobial Susceptibility Testing of Murraya koenigii extract on
Staphylococcus aureus
Supervisor(s)
Main: Ms Priya
Co- : Dr. Thet Thet Htar
Objective(s)
1. To determine the minimal inhibitory concentration value using disk diffusion method
and microdilution assay on Staphylococcus aureus cultures.
Abstract
Natural products have shown that they are potential source of antimicrobial
agents, such as neem leaves and curry leaves. Since antimicrobial therapy is going
through a crisis with the increasing resistance towards antimicrobial agents, it is
important to screen new plants and study their effectiveness in their antimicrobial
activities.
Staphylococcus aureus, gram positive bacteria that typically occur in grape-like
clusters have yellow pigmented colonies. They seem to be the most pathogenic of all the
staphylococci and are often a problem in the hospital environment. Infections by
staphylococci have become difficult to treat as they have become resistant to many
antibiotics that are exposed in the hospital environment. Folliculitis, food poisoning, toxic
shock syndrome, impetigo are some of the diseases caused by S. aureus strains.
3. Title: Sub-chronic (28 days) toxicity study of Limonia acidissima bark extract in rats.
Supervisor(s)
Main: Dr. Chin Jin Han
Co-: Dr. Gabriel Akowuah
Objective(s)
1. To examine the possible toxic or adverse effect of L. acidissima bark extract in rats.
2. To determine LD 50 of Limonia acidissima extract.
Abstract
Limonia acidissima (Rutaceae) is commonly known as wood apple (locally
named belinggai), is distributed in dry warm regions of Burma, India, Malaya and Sri
Lanka. Oil and constituents derived from the leave, bark, roots and fruit pulp are used as
topical application on venomous wounds and against snakebite. An interesting feature of
the plants classified within the family Rutaceae is that they contain several types of
cytotoxic, antimicrobial, neuroactive quinoleic alkaloids derived from anthranilic acid. The
plant has been reported to contain limonoids in stem bark and root bark, alkaloids,
benzoquinone, flavonoids, triterpenoids in root bark, stem bark, leaves and fruits.
However, toxicity studies on this plant have not been reported extensively. Therefore,
this study aims to ensure the safe use of this plant for therapeutic purposes.
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