110 / 210 Fiber Optic Oxygen Monitor - Instech Laboratories, Inc.

4. Loosen the bracket thumbscrew and place the
bracket in the track.
5. Very carefully slide the probe through the hole in
the seal tightening screw and advance it until the
tip just protrudes into the chamber. Do not allow
the tip to touch the far side of the well, it could
jam and crack the tip.
6. The final position of the probe tip should have
just the angled portion of the tip exposed with
the bevel facing upward. As the seal is tightened,
it may advance into the chamber. Tighten seal
and then reposition as necessary until the seal no
longer can be moved.
Chamber Plugs
Depending upon the chamber size, different style
plugs may be use, either beveled tip style or O-ring
style with single vertical fill hole.
The beveled type can be rotated to seal off the side
fill hole.
For large volume chambers, e.g. 1000 microliters, the
central hole can be plugged to reduce oxygen leakage
although little reoxygenation actually occurs.
Larger chambers have no side fill hole.
Setting the Optional Beveled Plug Depth
1. Slide a fine tip micro pipette tip into the fill hole
until it touches the far side of the chamber well.
2. Drop the beveled transparent plug into the well
with the bevel matching the angle of the pipette
tip.
3. Place the collar over the plug and tighten the
holding screw facing forward. This will be the
position indicator as well.
4. This process will position the depth of the plug
so that air will clear properly when the chamber
is initially filled
7. Repeat for the second chamber and then attach
the single end of each bifurcated fiber bundle.
Using the Titanium Micro
Chamber with Beveled Plug
1. Fill the chamber with buffer solution that has
been equilibrated at your operating temperature
with the oxygen tension to be used in the
experiment. Use slightly more than the specified
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