110 / 210 Fiber Optic Oxygen Monitor - Instech Laboratories, Inc.

volume of the chamber. A small overflow will
occur.
2. If working at ambient pO 2, you may allow some
time to allow thermal and oxygen equilibration
to occur before sealing off the sample.
3. Add cells or organelles and slide in the central
transparent core plug. Orient the top collar so
that the air rises to the top of the bevel and exits
through the fill port.
4. The setscrew should be facing you when the plug
has been correctly installed.
5. Turn off stirring, if on.
6. Slowly continue to slide the plug down while
watching from above to ensure that all air is
expelled from the chamber.
7. When no air bubbles are visible, rotate the top
collar until the setscrew faces toward the rear
which will seal the chamber.
8. Additions can made though the fill port in the
right front of the chamber. First return the
plug/valve to the forward position to allow entry.
9. This should be done with a loosely fitting needle
or pipette so that the overflow will flow by the
needle. No separate vent port is needed. Inject
near the bottom and the overflow will flow out
from the top part of the chamber and into the
circular trough. Turning the stirrer off during this
procedure is recommended to reduce mixing.
Using the Titanium
Chamber with O-ring fitted
plugs with central fill hole.
This type does not depend on the use of the side fill
hole and may not even exist with larger chambers.
1. Fill chamber with plug out to slightly more than
the stated chamber volume.
2. Gently push plug into chamber. Air should be
expelled and a small amount of solution will be
expelled insuring that no bubbles remain.
3. Continue as in step 9 above.
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