Goat anti-Rabbit IgG H&L (AP) secondary antibody (ab97048) - Abcam
Goat anti-Rabbit IgG H&L (AP) secondary antibody (ab97048) - Abcam
Goat anti-Rabbit IgG H&L (AP) secondary antibody (ab97048) - Abcam
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Product Datasheet<br />
<strong>Goat</strong> Anti-<strong>Rabbit</strong> <strong>IgG</strong> H&L (Alkaline<br />
Phosphatase) <strong>ab97048</strong><br />
1 References 画 像 数 1<br />
Overview<br />
Product name<br />
Description<br />
Target species<br />
Tested applications<br />
Conjugation<br />
Properties<br />
<strong>Goat</strong> Anti-<strong>Rabbit</strong> <strong>IgG</strong> H&L (Alkaline Phosphatase)<br />
<strong>Goat</strong> polyclonal Secondary Antibody to <strong>Rabbit</strong> <strong>IgG</strong> - H&L (<strong>AP</strong>)<br />
<strong>Rabbit</strong><br />
ICC, IHC-P, ELISA, WB<br />
Alkaline Phosphatase<br />
Form<br />
Storage instructions<br />
Storage buffer<br />
Purity<br />
Purification notes<br />
Liquid<br />
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.<br />
Preservative: 0.09% Sodium Azide<br />
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc<br />
chloride, pH 7.1<br />
Immunogen affinity purified<br />
Conjugation notes Molar enzyme: <strong>anti</strong>body protein ratio is 1:1<br />
Clonality<br />
Isotype<br />
General notes<br />
This <strong>anti</strong>body was isolated by affinity chromatography using <strong>anti</strong>gen coupled to agarose beads and conjugated to<br />
Alkaline Phosphatase (<strong>AP</strong>).<br />
Polyclonal<br />
<strong>IgG</strong><br />
Part of the AbExcel range.<br />
Research Areas Immunology Immunoglobulins Heavy Chain <strong>IgG</strong><br />
Secondary <strong>anti</strong>bodies <strong>anti</strong>-<strong>Rabbit</strong> <strong>IgG</strong> Enzyme Alkaline Phos<br />
Applications<br />
Our Abpromise guarantee covers the use of <strong>ab97048</strong> in the following tested applications.<br />
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.<br />
Application<br />
ICC<br />
Notes<br />
ICC: Use at an assay dependent dilution.<br />
IHC-P IHC-P: 1/100 - 1/1000.<br />
ELISA<br />
ELISA: 1/5000 - 1/50000. Primary<br />
WB<br />
WB: 1/5000 - 1/50000. Chemiluminescent detection<br />
<strong>Goat</strong> Anti-<strong>Rabbit</strong> <strong>IgG</strong> H&L (Alkaline Phosphatase) images<br />
http://www.abcam.co.jp/<strong>Goat</strong>-<strong>Rabbit</strong>-<strong>IgG</strong>-HL-Alkaline-Phosphatase-<strong>ab97048</strong>.html 1
Product Datasheet<br />
All lanes : Anti-beta Actin <strong>anti</strong>body - Loading<br />
Control (ab8227) at 1 µg/ml<br />
Lanes 1-6 : HeLa (Human epithelial<br />
carcinoma cell line) Whole Cell Lysate<br />
Lysates/proteins at 10 µg per lane.<br />
Western blot - <strong>Goat</strong> <strong>anti</strong>-<strong>Rabbit</strong> <strong>IgG</strong> H&L<br />
(<strong>AP</strong>) <strong>secondary</strong> <strong>anti</strong>body (<strong>ab97048</strong>)<br />
Secondary<br />
Lanes 1 - 2 : <strong>Goat</strong> polyclonal Secondary<br />
Antibody to <strong>Rabbit</strong> <strong>IgG</strong> - H&L (<strong>AP</strong>) (<strong>ab97048</strong>)<br />
at 1/5000 dilution<br />
Lanes 3 - 4 : <strong>Goat</strong> polyclonal Secondary<br />
Antibody to <strong>Rabbit</strong> <strong>IgG</strong> - H&L (<strong>AP</strong>) (<strong>ab97048</strong>)<br />
at 1/20000 dilution<br />
Lanes 5 - 6 : <strong>Goat</strong> polyclonal Secondary<br />
Antibody to <strong>Rabbit</strong> <strong>IgG</strong> - H&L (<strong>AP</strong>) (<strong>ab97048</strong>)<br />
at 1/50000 dilution<br />
Developed using the ECL technique<br />
Performed under reducing conditions.<br />
Exposure time : 4 minutes<br />
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THER<strong>AP</strong>EUTIC USE"<br />
Our Abpromise to you: Quality guaranteed and expert technical support<br />
As standard<br />
Replacement or refund for products not performing as stated on the datasheet<br />
Valid for 12 months from date of delivery**<br />
Response to your inquiry within 24 hours<br />
In addition<br />
We provide support in Chinese, English, French, German, Japanese and Spanish<br />
Extensive multi-media technical resources to help you<br />
We investigate all quality concerns to ensure our products perform to the highest standards<br />
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,<br />
please visit http://www.abcam.com/abpromise or contact our technical team.<br />
Terms and conditions<br />
Guarantee only valid for products bought direct from <strong>Abcam</strong> or one of our authorized distributors<br />
**Regional variations to our Abpromise may apply to the following countries: China, Korea, Singapore, Malaysia, Taiwan and Thailand,<br />
which operate a 120 day guarantee. Please contact your regional office for further details<br />
Antibodies tested only in recombinant samples are not covered for use on endogenous samples. Please check the datasheet for<br />
information on tested species<br />
"Predicted to react" information on datasheets is based on sequence homology and provided for reference only<br />
"Predicted to react" species are not guaranteed, as they have not been tested<br />
Fast Track products are not covered under the Abpromise. Please see Fast Track terms and condition for additional information<br />
<strong>Abcam</strong> biochemicals are novel compounds and we have not tested their biological activity in house. Please use the literature to identify<br />
http://www.abcam.co.jp/<strong>Goat</strong>-<strong>Rabbit</strong>-<strong>IgG</strong>-HL-Alkaline-Phosphatase-<strong>ab97048</strong>.html 2
Product Datasheet<br />
how to use these products effectively. If you require further assistance please contact the scientific support team<br />
Japan Office<br />
アブカム 株 式 会 社<br />
〒103-0012 東 京 都 中 央 区 日 本 橋 堀 留 町 2-2-1 住 友 不 動 産 人 形 町 ビル2F<br />
Tel: +81-(0)3-6231-0940<br />
Fax: +81-(0)3-6231-0941<br />
Email: orders@abcam.co.jp<br />
Visit us at: www.abcam.co.jp<br />
http://www.abcam.co.jp/<strong>Goat</strong>-<strong>Rabbit</strong>-<strong>IgG</strong>-HL-Alkaline-Phosphatase-<strong>ab97048</strong>.html 3
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