poster - International Conference of Agricultural Engineering

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evaluation, five central plants, amounting to 20 plants per treatment. The results were subjected to analysis of variance. Variables significant at Test F were subjected to the Tukey test at 5% probability. 3. Results and discussion The water management affect with different intensities the morphological characteristics of Eucalyptus grandis seedlings (Table 1). Only the seedlings without stress treatment (T1) differed significantly from the others, with higher rates. The fact that the height was similar among stress treatments it can be attributed to the fact that during hardening, seedlings in tube have gone through the phase of rapid growth, since the size of the pack and hence the amount of substrate and nutrients are limiting (Silva et al., 2004). Therefore the seedlings that suffered water stress did not have higher growth rates than those subjected to high stress. Sasse et al. (1996) and Coopman et al. (2008) found a reduction of the height of Eucalyptus globulus subjected to water stress and Lopes et al. (2007) found an increase in height of of Eucalyptus grandis seedlings with increasing levels of irrigation. The stem diameter was not associated with the level of applied stress as found by Coopman et al. (2008) and different in Sasse et al. (1996) who concluded that diameter growth rates were reduced by water stress. The shoot dry masses, root dry masses and total dry masses had decreasing mean inasmuch water stress levels increased. Although the root dry masses of T1, considering only the root system contained in the tube (as seedlings are in constant contact with water, the root system grew out of the tube), had statistically similar values to those subjected to higher stress. These results confirm that the decrease of water promotes a slower growth which reflects in the total dry matter produced. The presence of water in the tissues of the leaves causes the stomatal cells to become turgid for longer and open capturing light energy and carbon to photosynthesize. Coopman et al. (2008) found a reduction in the biomass of leaf, stem and root of Eucalyptus globulus seedlings when subjected to water stress. Lima et al. (2005) observed that the increase of irrigation led to the linear increase in root biomass of Eucalyptus grandis seedlings. The results of the leaf area of seedlings showed that water stress also affected leaf area. In T1 it was observed that despite presenting greater height, the internodes were higher, with the leaves more spaced out contributing for a less high leaf area than others. The treatments with higher levels of stress (T4 and T5) showed the lowest values of leaf area did not differing from each other. Lopes et al. (2007) reported an increase in leaf area with increasing levels of irrigation. Stoneman et al. (1994) and Coopman et al. (2008) found a reduction in leaf area of Eucalyptus marginata and globulus respectively, when subjected to water stress. The most stressed seedlings (T5) had the lowest specific leaf weight. Treatment 1 had the highest specific leaf weight and was statistically different from the other treatments. Farrell et al. (1996) in his research on physiological and morphological responses of seedlings of six clones of Eucalyptus camaldulensis grown in a greenhouse and subjected to water stress treatments found that clones that produced a large number of leaves had leaves with low specific weight, while clones that produced a few leaves had leaves with relatively high specific weight.

Table1. Morphological characteristics of Eucalyptus grandis seedlings, 100 days after sowing, under different irrigation management during hardening. Trat. H (cm) D (mm) SDM (g) RDM (g) TDM (g) LA (m 2 ) LSW (g m -2 ) T1 70.53 a 3.49 b 1.96 a 0.51 ab 2.47 a 0.0166 ab 101.30 a T2 52.15 b 3.63 ab 2.03 a 0.59 a 2.62 a 0.0173 a 87.74 b T3 48.95 b 3.79 a 1.83 ab 0.56 ab 2.39 ab 0.0182 a 84.45 bc T4 51.25 b 3.59 ab 1.61 b 0.50 b 2.12 b 0.0142 c 82.06 bc T5 50.95 b 3.46 b 1.56 b 0.50 b 2.06 b 0.0144 bc 73.43 c CV 7.75 8.87 18.24 16.61 16.88 16,32 16.31 Means followed by same letters in the same column do not differ by the Tukey Test at 5% level of significance. CV = coefficient of variation. T1: Seedlings without suffering water stress (under continuous subirrigation), T2, T3, T4 and T5: irrigated seedlings up to reaching a tension of the substrate water retention at -50, -100, - 500 and -1500 KPa, respectively. 4. Conclusion It is concluded that water management influence on the quality of Eucalyptus grandis seedlings, as it altered morphological characteristics. Although the greatest differences occurred among the seedlings grown under continuous subirrigation and those which suffered some level of stress during hardening. Acknowledgements: This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), a Brazilian Government Agencies. 5. References Coopman, R.E. et al. (2008). Changes in morpho-physiological attributes of Eucalyptus globulus plants in response to different drought hardening treatments. Electronic Journal of Biotechnology, 11, 1-10. Costa e Silva, F. et al. (2004). Responses to water stress in two Eucalyptus globulus clones differing in drought tolerance. Tree Physiology, 24, 1165–1172. Farrell, R.C.C., Bell, D.T., Akilan, K. & Marshall, J.K. (1996). Morphological and physiological comparisons of clonal lines of Eucalyptus camaldulensis. I. Responses to drought and waterlogging. Australian J ournal of Plant Physiology, 23, 497-507. Lemcoff, J.H., Garau, A., Guarnaschelli, A. & Prystupa, P. (1997). Water stress in seedlings of Eucalyptus camaldulensis clones and its efects on growth characteristics. In: IUFRO Conference on Silviculture and Improvement of Eucalypt. Proceedings. Colombo: EMBRAPA - Centro Nacional de Pesquisa de Florestas.

evaluation, five central plants, amounting to 20 plants per treatment. The results were<br />

subjected to analysis <strong>of</strong> variance. Variables significant at Test F were subjected to the Tukey<br />

test at 5% probability.<br />

3. Results and discussion<br />

The water management affect with different intensities the morphological<br />

characteristics <strong>of</strong> Eucalyptus grandis seedlings (Table 1). Only the seedlings without stress<br />

treatment (T1) differed significantly from the others, with higher rates.<br />

The fact that the height was similar among stress treatments it can be attributed to the<br />

fact that during hardening, seedlings in tube have gone through the phase <strong>of</strong> rapid growth,<br />

since the size <strong>of</strong> the pack and hence the amount <strong>of</strong> substrate and nutrients are limiting (Silva<br />

et al., 2004). Therefore the seedlings that suffered water stress did not have higher growth<br />

rates than those subjected to high stress.<br />

Sasse et al. (1996) and Coopman et al. (2008) found a reduction <strong>of</strong> the height <strong>of</strong><br />

Eucalyptus globulus subjected to water stress and Lopes et al. (2007) found an increase in<br />

height <strong>of</strong> <strong>of</strong> Eucalyptus grandis seedlings with increasing levels <strong>of</strong> irrigation.<br />

The stem diameter was not associated with the level <strong>of</strong> applied stress as found by<br />

Coopman et al. (2008) and different in Sasse et al. (1996) who concluded that diameter<br />

growth rates were reduced by water stress.<br />

The shoot dry masses, root dry masses and total dry masses had decreasing mean<br />

inasmuch water stress levels increased. Although the root dry masses <strong>of</strong> T1, considering<br />

only the root system contained in the tube (as seedlings are in constant contact with water,<br />

the root system grew out <strong>of</strong> the tube), had statistically similar values to those subjected to<br />

higher stress. These results confirm that the decrease <strong>of</strong> water promotes a slower growth<br />

which reflects in the total dry matter produced.<br />

The presence <strong>of</strong> water in the tissues <strong>of</strong> the leaves causes the stomatal cells to become<br />

turgid for longer and open capturing light energy and carbon to photosynthesize. Coopman et<br />

al. (2008) found a reduction in the biomass <strong>of</strong> leaf, stem and root <strong>of</strong> Eucalyptus globulus<br />

seedlings when subjected to water stress. Lima et al. (2005) observed that the increase <strong>of</strong><br />

irrigation led to the linear increase in root biomass <strong>of</strong> Eucalyptus grandis seedlings.<br />

The results <strong>of</strong> the leaf area <strong>of</strong> seedlings showed that water stress also affected leaf<br />

area. In T1 it was observed that despite presenting greater height, the internodes were<br />

higher, with the leaves more spaced out contributing for a less high leaf area than others.<br />

The treatments with higher levels <strong>of</strong> stress (T4 and T5) showed the lowest values <strong>of</strong> leaf area<br />

did not differing from each other.<br />

Lopes et al. (2007) reported an increase in leaf area with increasing levels <strong>of</strong> irrigation.<br />

Stoneman et al. (1994) and Coopman et al. (2008) found a reduction in leaf area <strong>of</strong><br />

Eucalyptus marginata and globulus respectively, when subjected to water stress.<br />

The most stressed seedlings (T5) had the lowest specific leaf weight. Treatment 1<br />

had the highest specific leaf weight and was statistically different from the other treatments.<br />

Farrell et al. (1996) in his research on physiological and morphological responses <strong>of</strong><br />

seedlings <strong>of</strong> six clones <strong>of</strong> Eucalyptus camaldulensis grown in a greenhouse and subjected to<br />

water stress treatments found that clones that produced a large number <strong>of</strong> leaves had leaves<br />

with low specific weight, while clones that produced a few leaves had leaves with relatively<br />

high specific weight.

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