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information is required to establish the control measures to reduce ARB load in wastewaters and thus its environmental dissemination. 4. Acknowledgments This work is funded by National Funds through the FCT (Portuguese Foundation for Science and Technology) under the project PEst-OE/AGR/UI0681/2011. 5. References Amador, P., Fernandes, R., Duarte, I., Brito, L. & Prudêncio, C. (2011). In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods. World Journal of Microbiology and Biotechnology, 27, 1775-1785. Aminov, R.I. (2009). The role of antibiotics and antibiotic resistance in nature. Environmental Microbiology, 11, 2970-2988. Anónimo (2007). Decreto-Lei No 306/2007 de 27 de Agosto de 2007. Diário da República, 1ª série. Nº. 164 - 27 de Agosto de 2007. Pp. 5747-5765. Ding, C. & He, J. (2010). Effect of antibiotics in the environment on microbial populations. Applied Microbiology and Biotechnology, 87, 925-941. Falk, P., Hooper, L., Midtvedt, T. & Gordon, J. (1998). Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiol Mol Biol Rev, 62, 1157-1170. Fernandes, R. & Prudêncio, C. (2010). Post-surgical wound infections involving Enterobacteriaceae with reduced susceptibility to β-lactams in two Portuguese hospitals. International Wound Journal, 7, 508-514. Finch, R. & Hunter, P.A. (2006). Antibiotic resistance-action to promote new technologies: report of an EU Intergovernmental Conference held in Birmingham, UK, 12–13 December 2005. Journal of Antimicrobial Chemotherapy, 58, Suppl. S1, i3-i22. Jury, K.L., Vancov, T., Stuetz, R.M. & Khan, S.J. (2010). Antibiotic resistance dissemination and sewage treatment plants. In: Current Research, Technology and education Topics in Applied Microbiology and Microbial Biotechnology. Ed. Méndez-Vilas, A. Formatex. pp 509- 519. Kümmerer, K. (2004). Resistance in the environment. The Journal of Antimicrobial Chemotherapy, 54, 311-320. Kümmerer, K. (2009a). Antibiotics in the aquatic environment – A review – Part I. Chemosphere, 75, 417–434. Kümmerer, K. (2009b). Antibiotics in the aquatic environment – A review – Part II. Chemosphere, 75, 435–444. Livermore, D. M. & British Soc, A. (2011). Discovery research: the scientific challenge of finding new antibiotic. Journal of Antimicrobial Chemotherapy, 66, 1941-1944. Marecos do Monte, H. & Albuquerque, A. (2010). Reutilização de Águas Residuais. Série Guias Técnicos-14. Eds: Entidade Reguladora dos Serviços de Aguas e Resíduos e Instituto Superior de Engenharia de Lisboa. ISBN: 978-989-8360-01-4 Moore, J.E., Moore, P.J.A., Millar, B.C., Goldsmith, C.E., Loughrey, A., Rooney, P.J. & Rao, J.R. (2010). The presence of antibiotic resistant bacteria along the River Lagan. Agricultural Water Management, 98, 217–221. Moura, A., Carolina Pereira, C., Henriques, I. & Correia, A. (2011). Novel gene cassettes and integrons in antibiotic-resistant bacteria isolated from urban wastewaters. Research in Microbiology, 163, 92-100. NCCLS (2005). Performance standard for antimicrobial susceptibility testing; Clinical and Laboratory Standards Institute, Wayne, PA, USA, Supplement M100-S15. 5

Oliveira, A.B. & Henriques, M. (2011). Guia dos produtos fitofarmacêuticos. Lista dos produtos com venda autorizada. MADRP. DGADR. Schlüter, A., Heuer, H., Szczepanowski, R., Forney, L.J., Thomas, C.M., Pühler, A. & Top, E.M. (2003). The 64 508 bp IncP-1beta antibiotic multiresistance plasmid pB10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1beta group. Microbiology, 149, 3139-3153. Schwartz, T., Kohnen, W., Jansen, B. & Obst, U. (2003). Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS Microbiology Ecology, 43, 325-335. Silva, J., Castillo, G., Callejas, L., López, H. & Olmos, J. (2006). Frequency of transferable multiple antibiotic resistance amongst coliform bacteria isolated from a treated sewage effluent in Antofagasta, Chile. Electronic Journal of Biotechnology, 9, 533- 540. Watkinson, A.J., Murby, E.J., Kolpin, D.W. & Costanzo, S.D. (2009). The occurrence of antibiotics in an urban watershed: from wastewater to drinking water. Science of the Total Environment, 407, 2711-2723. West, B.M., Liggit, P., Clemans, D.L. & Francoeur, S.N. (2010). Antibiotic Resistance, Gene Transfer, and Water Quality Patterns Observed in Waterways near CAFO Farms and Wastewater Treatment Facilities. Water, Air, and Soil Pollution, 217, 473-489. Xi, C., Zhang, Y., Marrs, C., Ye, W., Simon, C., Forman, B. & Nriagu, J. (2009). Prevalence of antibiotic resistance in drinking water treatment and distribution systems. Applied and Environmental Microbiology, 75, 5714-5718. 6

information is required to establish the control measures to reduce ARB load in wastewaters<br />

and thus its environmental dissemination.<br />

4. Acknowledgments<br />

This work is funded by National Funds through the FCT (Portuguese Foundation for Science<br />

and Technology) under the project PEst-OE/AGR/UI0681/2011.<br />

5. References<br />

Amador, P., Fernandes, R., Duarte, I., Brito, L. & Prudêncio, C. (2011). In vitro transference<br />

and molecular characterization <strong>of</strong> bla TEM genes in bacteria isolated from Portuguese<br />

ready-to-eat foods. World Journal <strong>of</strong> Microbiology and Biotechnology, 27, 1775-1785.<br />

Aminov, R.I. (2009). The role <strong>of</strong> antibiotics and antibiotic resistance in nature. Environmental<br />

Microbiology, 11, 2970-2988.<br />

Anónimo (2007). Decreto-Lei No 306/2007 de 27 de Agosto de 2007. Diário da República, 1ª<br />

série. Nº. 164 - 27 de Agosto de 2007. Pp. 5747-5765.<br />

Ding, C. & He, J. (2010). Effect <strong>of</strong> antibiotics in the environment on microbial populations.<br />

Applied Microbiology and Biotechnology, 87, 925-941.<br />

Falk, P., Hooper, L., Midtvedt, T. & Gordon, J. (1998). Creating and maintaining the<br />

gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiol<br />

Mol Biol Rev, 62, 1157-1170.<br />

Fernandes, R. & Prudêncio, C. (2010). Post-surgical wound infections involving<br />

Enterobacteriaceae with reduced susceptibility to β-lactams in two Portuguese hospitals.<br />

<strong>International</strong> Wound Journal, 7, 508-514.<br />

Finch, R. & Hunter, P.A. (2006). Antibiotic resistance-action to promote new technologies:<br />

report <strong>of</strong> an EU Intergovernmental <strong>Conference</strong> held in Birmingham, UK, 12–13 December<br />

2005. Journal <strong>of</strong> Antimicrobial Chemotherapy, 58, Suppl. S1, i3-i22.<br />

Jury, K.L., Vancov, T., Stuetz, R.M. & Khan, S.J. (2010). Antibiotic resistance dissemination<br />

and sewage treatment plants. In: Current Research, Technology and education Topics in<br />

Applied Microbiology and Microbial Biotechnology. Ed. Méndez-Vilas, A. Formatex. pp<br />

509- 519.<br />

Kümmerer, K. (2004). Resistance in the environment. The Journal <strong>of</strong> Antimicrobial<br />

Chemotherapy, 54, 311-320.<br />

Kümmerer, K. (2009a). Antibiotics in the aquatic environment – A review – Part I.<br />

Chemosphere, 75, 417–434.<br />

Kümmerer, K. (2009b). Antibiotics in the aquatic environment – A review – Part II.<br />

Chemosphere, 75, 435–444.<br />

Livermore, D. M. & British Soc, A. (2011). Discovery research: the scientific challenge <strong>of</strong><br />

finding new antibiotic. Journal <strong>of</strong> Antimicrobial Chemotherapy, 66, 1941-1944.<br />

Marecos do Monte, H. & Albuquerque, A. (2010). Reutilização de Águas Residuais. Série<br />

Guias Técnicos-14. Eds: Entidade Reguladora dos Serviços de Aguas e Resíduos e<br />

Instituto Superior de Engenharia de Lisboa. ISBN: 978-989-8360-01-4<br />

Moore, J.E., Moore, P.J.A., Millar, B.C., Goldsmith, C.E., Loughrey, A., Rooney, P.J. & Rao,<br />

J.R. (2010). The presence <strong>of</strong> antibiotic resistant bacteria along the River Lagan.<br />

<strong>Agricultural</strong> Water Management, 98, 217–221.<br />

Moura, A., Carolina Pereira, C., Henriques, I. & Correia, A. (2011). Novel gene cassettes and<br />

integrons in antibiotic-resistant bacteria isolated from urban wastewaters. Research in<br />

Microbiology, 163, 92-100.<br />

NCCLS (2005). Performance standard for antimicrobial susceptibility testing; Clinical and<br />

Laboratory Standards Institute, Wayne, PA, USA, Supplement M100-S15.<br />

5

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