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datasheet - Dolomite Microfluidics

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The <strong>Dolomite</strong> Centre Ltd<br />

Unit 1, Anglian Business Park, Orchard Road,<br />

Royston, Hertfordshire, SG8 5TW, UK<br />

T: +44 (0)1763 242491<br />

F: +44 (0)1763 246125<br />

E: sales@dolomite-microfluidics.com<br />

W: www.dolomite-microfluidics.com<br />

<strong>Dolomite</strong> <strong>Microfluidics</strong> – North America Office<br />

Blacktrace Inc, 29 Albion Place<br />

Charlestown, MA 02129, USA<br />

C: 617 803 6655<br />

T: 617 848 1121<br />

F: 617 500 0136<br />

E: salesus@dolomite-microfluidics.com<br />

Droplet System Accessories<br />

Product <strong>datasheet</strong><br />

page<br />

Overview 2<br />

Droplet System Reagent Kit 2<br />

Custom Flow Resistor Kit 4<br />

Custom Flow Resistor Kit 0.8mm 5<br />

Droplet System Starter Kits 6<br />

The <strong>Dolomite</strong> Centre Limited Page 1 of 8


The <strong>Dolomite</strong> Centre Ltd<br />

Part name<br />

Part No.<br />

Droplet System Reagent Kit 3200048<br />

Custom Flow Resistor Kit 3200049<br />

Custom Flow Resistor Kit 0.8mm 3200309<br />

Pressure-based Droplet Starter Kit 3200075<br />

Syringe-based Droplet Starter Kit 3200076<br />

Advanced Droplet Starter Kit 3200077<br />

Droplet Collection Starter Kit 3200124<br />

Overview<br />

The Micro Droplet Systems are built around <strong>Dolomite</strong>’s industry leading pumps,<br />

connectors and chips. They provide a complete solution for the generation of<br />

monodispersed droplets in the size range 20 – 250µm. <strong>Dolomite</strong> also provides a range of<br />

flow accessories for use with the Micro Droplet Systems for quick set-up and<br />

experimentation.<br />

Droplet System Reagent Kit (Part No. 3200048)<br />

The Droplet System Reagent Kit contains a range of liquids for initial droplet<br />

demonstrations with a Droplet System. This provides users with the capability to start<br />

making droplets of oil-in-water or water-in-oil immediately. The reagent kit also contains<br />

two commonly used surfactants to increase droplet stability and chip cleaning fluid.<br />

30ml vial in Part No. 3200048<br />

Droplet System Reagent Kit<br />

Droplets of water with 10% v/v blue dye in mineral oil with 2% v/v<br />

Span 80 (surfactant) generated using fluids from reagent kit<br />

MAR-000070 1.1 Page 2 of 8


The <strong>Dolomite</strong> Centre Ltd<br />

Benefits:<br />

Contains the reagents needed to start making droplets<br />

Surfactants provided for droplet stability<br />

Chip cleaning fluid included<br />

Specifications:<br />

Reagent Kit Component Function Quantity Included<br />

30ml mineral oil in vial use as organic phase 2<br />

30ml filtered water in vial use as aqueous phase 1<br />

30ml filtered water with 10%<br />

v/v blue dye in vial<br />

use as aqueous phase 1<br />

30ml Span 80 in vial surfactant 1<br />

30ml Tween 80 in vial surfactant 1<br />

30ml Acetone in vial chip cleaning fluid 1<br />

30ml polypropylene vial with<br />

cap<br />

spare vials 5<br />

MAR-000070 1.1 Page 3 of 8


The <strong>Dolomite</strong> Centre Ltd<br />

Custom Flow Resistor Kit (Part No. 3200049)<br />

The Custom Flow Resistor Kit is designed for use with the Pressure-based Droplet Starter<br />

System. With the tools provided, flow resistors of the required length can be made from<br />

1.6mm (1/16”) OD tubing. This enables the user to set the desired flow rate ratio between<br />

the organic phase and the aqueous phase without independent pressure control of each<br />

phase. The additional benefit of flow resistors is that they maximize the flow rate range of<br />

the Mitos P-Pump and prevent flow rate instability when using fluids of different<br />

viscosities. The Custom Flow Resistor Kit contains high tolerance FEP tubing with 100 µm<br />

microbore and the required fittings to connect into the droplet system.<br />

¼-28 fitting and ferule ¼-28 union<br />

Precision Tube Cutter<br />

short flow resistor for high viscosity organic phase<br />

long flow resistor for low viscosity aqueous phase<br />

Example of Part No. 3200049 Custom Flow Resistor Kit used with Pressure-based Droplet Starter<br />

System<br />

MAR-000070 1.1 Page 4 of 8


The <strong>Dolomite</strong> Centre Ltd<br />

Benefits:<br />

Allows user to set the flow rate ratio without independent pressure control<br />

Maximises the flow rate range of the Mitos P-Pump<br />

Enables low flow rate performance for systems of low fluidic resistance<br />

Prevents flow rate instability when using fluids of different viscosities<br />

Quick to make and connect<br />

Excellent chemical compatibility<br />

Specifications:<br />

Custom Flow Resistor Kit<br />

Component<br />

Function<br />

Quantity Included<br />

FEP tubing 100µm ID (±25<br />

µm), 1.6mm (1/16”) OD, 10 m<br />

length<br />

use to make flow resistors 1<br />

Delrin ¼-28” end fitting with<br />

ETFE ferule for 1.6mm (1/16”)<br />

OD tubing<br />

Delrin ¼-28” female – female<br />

union<br />

Precision tube cutter<br />

use to connect flow resistor<br />

into system<br />

use to connect flow resistor<br />

into system<br />

use to produce clean 90º cut<br />

on FEP tubing<br />

5<br />

1<br />

1<br />

Custom Flow Resistor Kit 0.8mm (Part No. 3200309)<br />

The Custom Flow Resistor Kit 0.8mm allows flow resistors of the required length to be<br />

made from 0.8mm (1/32”) OD tubing.<br />

Custom Flow Resistor Kit<br />

0.8mm Component<br />

Function<br />

Quantity Included<br />

FEP tubing 100µm ID (±13<br />

µm), 0.8mm (1/32”) OD, 10 m<br />

length<br />

use to make flow resistors 1<br />

Delrin ¼-28” end fitting with<br />

ETFE ferule for 0.8mm (1/32”)<br />

OD tubing<br />

Delrin ¼-28” female – female<br />

union<br />

Precision tube cutter<br />

use to connect flow resistor<br />

into system<br />

use to connect flow resistor<br />

into system<br />

use to produce clean 90º cut<br />

on FEP tubing<br />

5<br />

1<br />

1<br />

MAR-000070 1.1 Page 5 of 8


3200073<br />

Pressure-based<br />

Droplet Starter Kit<br />

3200074<br />

Syringe-based<br />

Droplet Starter Kit<br />

3200075<br />

Advanced<br />

Droplet Starter Kit<br />

3200124<br />

Droplet Collection<br />

Starter Kit<br />

The <strong>Dolomite</strong> Centre Ltd<br />

Droplet System Starter Kits<br />

The Droplet System Starter Kits contain all the required tubing, fittings and accessories for<br />

carrying out experimentation with the micro droplet systems. With the starter kit it is quick<br />

and easy to set-up the droplet system and start making droplets. There is a separate<br />

starter kit for each of the micro droplet systems.<br />

Pressure-based Droplet System Starter Kit – Part No. 3200073<br />

Syringe-based Droplet System Starter Kit – Part No. 3200074<br />

Advanced Droplet System Starter Kit – Part No. 3200075<br />

Droplet Collection System Starter Kit – Part No. 3200124<br />

Droplet System<br />

Starter Kit Component<br />

Image<br />

T- Connector ETFE<br />

0.5mm I.D. for 1.6mm<br />

O.D. Pipe<br />

1 1 1 1<br />

Fluid Tube with fitting,<br />

FEP, 250µm ID<br />

(±25µm), 1/16” OD,<br />

0.5m length<br />

0 4 4 4<br />

Fluid Tube, FEP,<br />

250µm ID (±25µm),<br />

1/16” OD, 0.5m length<br />

2 2 2 2<br />

FEP Tubing 250µm ID<br />

(±25 µm), 1/16” OD, 1m<br />

length<br />

1 1 1 1<br />

MAR-000070 1.1 Page 6 of 8


The <strong>Dolomite</strong> Centre Ltd<br />

PTFE Tubing 500µm ID<br />

(±80 µm), 1/16” OD,<br />

10m length<br />

1 1 1 1<br />

Short length of PTFE<br />

Rod that can be used to<br />

block off ports on the<br />

Linear Connector or the<br />

Pipe Gripper. (Pack of<br />

10)<br />

0 1 1 1<br />

Delrin ¼-28” end fitting<br />

with ETFE ferule for<br />

1/16” tubing<br />

2 2 2 2<br />

Precision Tube Cutter 0 1 1 1<br />

¼-28” female thread to<br />

female Luer lock<br />

chemically resistant<br />

connector (Tefzel). For<br />

making connections<br />

between head fittings<br />

and syringes.<br />

1 1 1 1<br />

MAR-000070 1.1 Page 7 of 8


The <strong>Dolomite</strong> Centre Ltd<br />

MAR-000070 1.1 Page 8 of 8

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