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MICROSCOPE COMPONENTS<br />
FOR TRANSMITTED<br />
LIGHT APPLICATIONS<br />
Specifications and equipment are subject to change without any notice or<br />
obligation on the part of the manufacturer. June 2007. ©1997-07 NIKON CORPORATION<br />
NIKON CORPORATION<br />
Parale Mitsui Bldg., 8, Hig<strong>as</strong>hida-cho, Kaw<strong>as</strong>aki-ku,<br />
Kaw<strong>as</strong>aki, Kanagawa 210-0005, Japan<br />
phone: +81-44-223-2167 fax: +81-44-223-2182<br />
http://www.nikon-instruments.jp/eng/<br />
NIKON INSTRUMENTS INC.<br />
1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.<br />
phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only)<br />
fax: +1-631-547-0306<br />
http://www.nikonusa.com/<br />
NIKON INSTRUMENTS EUROPE B.V.<br />
P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands<br />
phone: +31-20-44-96-222 fax: +31-20-44-96-298<br />
http://www.nikon-instruments.com/<br />
NIKON INSTRUMENTS (SHANGHAI) CO., LTD.<br />
CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030<br />
(Beijing office) phone: +86-10-5869-2255 fax: +86-10-5869-2277<br />
(Guangzhou office) phone: +86-20-3882-0552 fax: +86-20-3882-0580<br />
NIKON SINGAPORE PTE LTD<br />
SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668<br />
NIKON MALAYSIA SDN. BHD.<br />
MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387<br />
NIKON INSTRUMENTS KOREA CO., LTD.<br />
KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415<br />
NIKON CANADA INC.<br />
CANADA phone: +1-905-625-9910 fax: +1-905-625-0103<br />
NIKON FRANCE S.A.S.<br />
FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33<br />
NIKON GMBH<br />
GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322<br />
NIKON INSTRUMENTS S.p.A.<br />
ITALY phone: +39-55-3009601 fax: +39-55-300993<br />
NIKON AG<br />
SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861<br />
NIKON UK LTD.<br />
UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584<br />
NIKON GMBH AUSTRIA<br />
AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40<br />
NIKON BELUX<br />
BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45<br />
Printed in Japan (0706-03)T Code No. 2CE-MUZH-3 This <strong>brochure</strong> is printed on recycled paper made from 40% used material.<br />
En
Contents<br />
System Diagram<br />
2<br />
System Diagram .............................. 3<br />
CFI 60 Objectives<br />
CFI Achromat Series<br />
for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DL)............ 4~5<br />
CFI Achromat 4x<br />
CFI Achromat 10x / CFI Achromat DL 10x<br />
CFI Achromat LWD 20x / CFI Achromat LWD DL 20x<br />
CFI Achromat 40x / CFI Achromat DL 40x<br />
CFI Achromat 60x<br />
CFI Achromat 100x Oil / CFI Achromat DL 100x Oil<br />
CFI Achromat LWD DL 20x F<br />
CFI Achromat LWD 40x C / CFI Achromat LWD DL 40x C<br />
CFI Plan Achromat Series<br />
for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DL) ............ 6~7<br />
CFI Plan Achromat UW 1x<br />
CFI Plan Achromat UW 2x<br />
CFI Plan Achromat 4x<br />
CFI Plan Achromat 10x / CFI Plan Achromat DL 10x<br />
CFI Plan Achromat 20x / CFI Plan Achromat DL 20x<br />
CFI Plan Achromat 40x / CFI Plan Achromat DL 40x<br />
CFI Plan Achromat 40x NCG<br />
CFI Plan Achromat 50x Oil<br />
CFI Plan Achromat 100x Oil / CFI Plan Achromat DL 100x Oil<br />
CFI Plan Achromat 100x NCG<br />
CFI Plan Fluor Series<br />
for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DLL) .......... 8~9<br />
CFI Plan Fluor 4x<br />
CFI Plan Fluor 10x / CFI Plan Fluor DLL 10x<br />
CFI Plan Fluor 20x / CFI Plan Fluor DLL 20x<br />
CFI Plan Fluor 40x / CFI Plan Fluor DLL 40x<br />
CFI Plan Fluor 40x Oil<br />
CFI Plan Fluor 60x C<br />
CFI Plan Fluor 100x Oil / CFI Plan Fluor DLL 100x Oil<br />
CFI Plan Fluor 100x Oil with iris diaphragm<br />
CFI Plan Fluor Series for Ph<strong>as</strong>e Contr<strong>as</strong>t (DL).... 9<br />
CFI Plan Fluor DL 4x<br />
CFI Plan Fluor DL 10x<br />
CFI Plan Fluor ELWD Series<br />
for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DM/DLL) ........ 10<br />
CFI Plan Fluor ELWD 20x C / CFI Plan Fluor ELWD DM 20x C<br />
CFI Plan Fluor ELWD 40x C / CFI Plan Fluor ELWD DM 40x C<br />
CFI Plan Fluor ELWD 60x C / CFI Plan Fluor ELWD DLL 60x C<br />
Multi-Immersion Objective for Brightfield .....<br />
CFI Plan Fluor 20x MI<br />
CFI Plan Apochromat Series<br />
10<br />
for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DM) ......... 11~12<br />
CFI Plan Apochromat 2x<br />
CFI Plan Apochromat 4x<br />
CFI Plan Apochromat 10x<br />
CFI Plan Apochromat 20x / CFI Plan Apochromat DM 20x<br />
CFI Plan Apochromat 40x C / CFI Plan Apochromat DM 40x C<br />
CFI Plan Apochromat 40x Oil / CFI Plan Apochromat DM 40x Oil<br />
CFI Plan Apochromat 60x C / CFI Plan Apochromat DM 60x C<br />
CFI Plan Apochromat 60x Oil / CFI Plan Apochromat DM 60x Oil<br />
CFI Plan Apochromat 60x WI<br />
CFI Plan Apochromat 100x Oil / CFI Plan Apochromat DM 100x Oil<br />
CFI Plan Apochromat 100x NCG Oil<br />
CFI S Fluor Series.......................................... 13<br />
CFI S Fluor 10x<br />
CFI S Fluor 20x<br />
CFI S Fluor 40x C<br />
CFI S Fluor 40x Oil<br />
CFI S Fluor 100x Oil<br />
Water Dipping Series.................................... 14<br />
CFI Fluor 10x W<br />
CFI Fluor 40x W<br />
CFI Fluor 60x W<br />
CFI HMC Series<br />
for Hoffmann Modulation Contr<strong>as</strong>t ® .............. 15<br />
CFI HMC 10x<br />
CFI HMC 20x F<br />
CFI HMC LWD 40x C<br />
2nd Objective Lens Unit ............................... 15<br />
Markings on CFI60 Objectives........................ 16<br />
17<br />
Condensers....................................<br />
C-C Abbe Condenser, Dry Type<br />
C-C Achromat Condenser<br />
Achromat Swing-out Condenser<br />
C-C Achromat /Aplanat Condenser<br />
LWD Achromat Condenser<br />
C-C Low Power Condenser<br />
Dark Field Condenser, Oil<br />
Dark Field Condenser, Dry<br />
C-CEL Expander Lens (for darkfield)<br />
Illumination Systems ................ 18~19<br />
Lamphouse HMX-3B<br />
Lamphouse HMX-4B, built-in back mirror<br />
Lamphouse HMX<br />
Mercury Lamp Socket S 100W for HMX-3B<br />
Halogen Lamp Socket 100W/HMX<br />
C-FC Epi-Fl Collector Lens<br />
Quartz Epi-Fl Collector Lens<br />
Collector Lens/QH100W<br />
C-LA HMX Lamphouse Adapter<br />
HMX Connecting Adapter SB-X1 (Bayonet to Screw)<br />
HMX Connecting Adapter SB-X2 (Screw to Bayonet)<br />
Eyepiece Tubes .............................. 19<br />
Y-TB Binocular Tube<br />
Y-TE Ergonomic Binocular Tube<br />
Y-TF Trinocular Tube “F” UW<br />
Y-TT Trinocular Tube “T” UW<br />
Intermediate Tubes ....................... 20<br />
Y-FL Epi-Fl Attachment<br />
Y-IDP Double Port<br />
Eyepieces......................................<br />
CFI 10x with diopter adjustment<br />
CFI 10x M Photom<strong>as</strong>k with diopter adjustment<br />
CFI 12.5x with diopter adjustment<br />
CFI 15x with diopter adjustment<br />
20<br />
Nosepiece ...................................... 21<br />
V-NA Motorized Sextuple Nosepiece<br />
V-ND MG-DIC Intelligent Sextuple Nosepiece<br />
C-N Sextuple Nosepiece<br />
C-ND Sextuple DIC Nosepiece<br />
CCTV Camera Adapters ............. 22~23<br />
V-T Photo Adapter<br />
Y-T TV Tube for C-Mount Adapter A<br />
C-Mount TV Adapter A<br />
Relay Lens 1x I<br />
C-Mount TV Adapter<br />
ENG-Mount TV Adapter<br />
Zoom Lens<br />
C-Mount Zooming Adapter<br />
ENG-Mount Zooming Adapter<br />
C-Mount TV Adapter 0.45x<br />
C-Mount TV Adapter 0.6x<br />
ENG-Mount TV Adapter 0.45x (for Trinocular Tube “F”, “T”)<br />
ENG-Mount TV Adapter 0.6x (for Trinocular Tube “F”, “T”)<br />
C-Mount TV Adapter VM 2.5x<br />
C-Mount TV Adapter VM 4x<br />
Mercury Lamp<br />
Socket S 100W<br />
Halogen Lamp<br />
Socket 100W/HMX<br />
C-C Abbe Condenser<br />
(Dry)<br />
ENG-Mount CCTV Camera<br />
ENG-Mount<br />
TV Adapter<br />
ENG-Mount<br />
TV<br />
Adapter*1*2<br />
0.45X, 0.6X<br />
Y-T TV Tube<br />
for C-Mount<br />
Adapter A<br />
Lamphouse HMX-3B<br />
Lamphouse HMX-4B<br />
with Built-in Back Mirror<br />
Lamphouse HMX<br />
ENG-<br />
Mount<br />
Zooming<br />
Adapter<br />
Relay<br />
Lens 1X I<br />
V-T Photo Adapter<br />
C-C Achromat Condenser<br />
C-Mount<br />
Zooming<br />
Adapter<br />
Zoom Lens<br />
Illumination Systems<br />
C-LA HMX Lamphouse Adapter<br />
HMX Connecting Adapter SB-X1<br />
(Bayonet to Screw Mount)<br />
HMX Connecting Adapter SB-X2<br />
(Screw to Bayonet Mount)<br />
C-FC Epi-Fl Collector Lens<br />
Quartz Epi-Fl Collector Lens<br />
Collector Lens/QH 100W<br />
Achromat Swing-out<br />
Condenser<br />
C-Mount TV<br />
Adapter<br />
Relay Lens<br />
1X I<br />
C-C Achromat/Aplanat<br />
Condenser<br />
C-Mount CCTV Camera<br />
C-Mount<br />
TV Adapter<br />
0.45X, 0.6X<br />
Condensers<br />
C-Mount<br />
TV<br />
Adapter A<br />
Y-TT Trinocular Tube "T" UW Y-TF Trinocular Tube "F" UW Y-TB Binocular Tube Y-TE Ergonomic<br />
Binocular Tube<br />
V-NA<br />
Motorized<br />
Sextuple Nosepiece<br />
LWD Achromat<br />
Condenser<br />
C-Mount<br />
TV Adapter<br />
VM2.5X<br />
CFI 10X<br />
with Diopter Adjustment<br />
C-Mount TV<br />
Adapter A<br />
C-Mount<br />
TV Adapter<br />
VM4X<br />
CFI 10XM<br />
Photom<strong>as</strong>k with<br />
Diopter Adjustment<br />
Eyepiece Tubes<br />
Y-FL Epi-Fl Attachment<br />
V-ND<br />
MG-DIC Intelligent<br />
Sextuple Nosepiece<br />
C-C Low Power<br />
Condenser<br />
C-Mount<br />
TV Adapter A<br />
CFI 12.5X<br />
with Diopter Adjustment<br />
Y-IDP Double Port<br />
Intermediate Tubes<br />
CCTV Camera Adapters<br />
*1: Use the dedicated 0.45X adapter for the double port sub-port.<br />
The 0.6X adapter cannot be used with the double port sub-port.<br />
*2: ENG-mount CCTV adapter 0.45x is a 38-bayonet mount type;<br />
other ENG-mount CCTV adapters are 48-bayonet mount types.<br />
C-N<br />
Sextuple<br />
Nosepiece<br />
CFI60 Objectives<br />
Darkfield Condenser (Oil)<br />
C-CEL Expander Lens<br />
Eyepieces<br />
CFI 15X<br />
with Diopter Adjustment<br />
Intermediate Tubes<br />
Nosepieces<br />
C-ND DIC<br />
Nosepiece<br />
Darkfield Condenser<br />
(Dry)<br />
3
CFI60 OBJECTIVES<br />
CFI Achromat Series for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DL)<br />
Correction for chromatic aberration in these objectives have been dramatically improved and are at the same level<br />
<strong>as</strong> the CFI Plan Achromat Series. These objectives were also corrected for spherical aberration and coma and<br />
image flatness across the 22mm field of view h<strong>as</strong> been dr<strong>as</strong>tically improved.<br />
The result: truly exceptional quality for this cl<strong>as</strong>s of objectives.<br />
The DL ph<strong>as</strong>e contr<strong>as</strong>t objectives in this series have more contr<strong>as</strong>t than the DLL objectives for enhanced contr<strong>as</strong>t<br />
with unstained specimens.<br />
ø27<br />
M25~0.75<br />
ø27<br />
M25~0.75<br />
ø25<br />
ø17<br />
ø14.3<br />
ø8.1<br />
ø28.2<br />
ø25.5<br />
ø20<br />
ø12.2<br />
4.5<br />
ø27<br />
M25~0.75<br />
4.5<br />
4.5<br />
45˚<br />
4.2<br />
60.41<br />
D 20X /0.4<br />
Ph1 DL<br />
/1.2 WD 3.0<br />
M25~0.75<br />
ø27<br />
45˚<br />
6<br />
35˚<br />
3 4.5<br />
19.9 10<br />
30˚<br />
C1<br />
4 X /0.10<br />
/- WD 30<br />
ø25<br />
60.06<br />
2.3 5 36.5<br />
10<br />
45˚<br />
45˚<br />
10X /0.25<br />
/- WD 6.1<br />
ø10<br />
ø16<br />
ø25<br />
60.06<br />
4.1 6 36<br />
10<br />
45˚<br />
45˚<br />
20X /0.40<br />
/0.17 WD 3.8<br />
ø8<br />
ø14.3<br />
ø17<br />
ø25<br />
60.06<br />
4.5 10<br />
36<br />
60.41<br />
D 40X /0.55<br />
WD 2.1<br />
/0–2<br />
2 1.6 1.2 0.8 0.4 0<br />
M25~0.75<br />
ø29<br />
ø32<br />
ø34<br />
5 24.6 25<br />
CFI Achromat 4x<br />
CFI Achromat 10x<br />
CFI Achromat DL 10x<br />
CFI Achromat LWD 20x<br />
CFI Achromat LWD DL 20x<br />
CFI Achromat LWD DL 20x F<br />
CFI Achromat LWD 40x C<br />
CFI Achromat LWD DL 40x C<br />
ø27<br />
M25~0.75<br />
ø27<br />
M25~0.75<br />
ø27<br />
M25~0.75<br />
3.7 3 8<br />
34.5<br />
10 5<br />
45˚<br />
35˚<br />
40X /0.65<br />
WD 0.65<br />
/0.17<br />
ø7<br />
ø13<br />
ø17<br />
ø20<br />
ø25<br />
60.06<br />
CFI Achromat 40x<br />
CFI Achromat DL 40x<br />
5<br />
3 8<br />
34.5<br />
10<br />
4.1<br />
45˚<br />
35˚<br />
60X /0.80<br />
/0.17 WD 0.3<br />
ø7<br />
ø13<br />
ø17<br />
ø20<br />
ø25<br />
CFI Achromat 60x<br />
60.06<br />
5<br />
5 8<br />
34.5<br />
10<br />
2.2<br />
45˚<br />
20˚<br />
00X / 1.25 oil<br />
/0.17 WD 0.18<br />
ø5<br />
ø13<br />
ø16.6<br />
ø20<br />
ø25<br />
60.06<br />
CFI Achromat 100x Oil<br />
CFI Achromat DL 100x Oil<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Ph<strong>as</strong>e ring<br />
CFI Achromat 4x 0.10 30.0 50.0 27.50 72<br />
CFI Achromat 10x 0.25 6.1 20.0 4.40 85<br />
CFI Achromat DL 10x 0.25 6.1 20.0 4.40 85 ph1<br />
CFI Achromat LWD 20x 0.40 3.8 10.0 1.72 90<br />
CFI Achromat LWD DL 20x 0.40 3.8 10.0 1.72 90 ph1<br />
CFI Achromat 40x 0.65 0.65 5.0 0.65 130 Spring-loaded<br />
CFI Achromat DL 40x 0.65 0.65 5.0 0.65 130 Spring-loaded ph2<br />
CFI Achromat 60x 0.80 0.3 3.3 0.43 130 Spring-loaded<br />
CFI Achromat 100x Oil 1.25 0.18 2.0 0.27 130 Spring-loaded<br />
CFI Achromat DL 100x Oil 1.25 0.18 2.0 0.27 130 Spring-loaded ph3<br />
CFI Achromat LWD DL 20x F 0.40 3.0 10.0 1.72 90 Cover gl<strong>as</strong>s thickness 1.2mm ph1<br />
CFI Achromat LWD 40x C 0.55 2.7–1.7 5.0 0.91 195 Cover gl<strong>as</strong>s correction 0.2mm<br />
CFI Achromat LWD DL 40x C 0.55 2.7–1.7 5.0 0.91 195 Cover gl<strong>as</strong>s correction 0.2mm ph2<br />
4<br />
5
CFI60 OBJECTIVES<br />
CFI Plan Achromat Series for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DL)<br />
These objectives provide incredible image flatness over the entire 25mm field of view, with chromatic aberration<br />
corrected throughout the entire visible spectrum. These objectives are suitable not only for routine laboratory work<br />
but for photomicrography.<br />
The DL ph<strong>as</strong>e contr<strong>as</strong>t objectives in this series have more contr<strong>as</strong>t than the DLL objectives for enhanced contr<strong>as</strong>t<br />
with unstained specimens.<br />
10.7 46<br />
5<br />
ø30<br />
M25~0.75<br />
Plan UW<br />
1X /0.04<br />
/-WD 3.2<br />
60.06<br />
52.4 5<br />
5<br />
ø30<br />
M25~0.75<br />
Plan UW<br />
2X /0.06<br />
/-WD 7.5<br />
60.06<br />
19.9 10 4.5<br />
ø27<br />
M25~0.75<br />
Plan 4X/0.<br />
/-WD 3<br />
60.06<br />
5.5 3.5<br />
50.5<br />
5.3<br />
3<br />
45˚<br />
45˚<br />
ø30<br />
M25~0.75<br />
Plan<br />
40 X /0.65 N<br />
/ 0 WD 0.48<br />
ø7.5<br />
ø14<br />
ø18.8<br />
60<br />
CFI Plan Achromat 40x NCG<br />
ø27<br />
M25~0.75<br />
4.5 4.3 51.2<br />
5.3<br />
45˚<br />
30˚<br />
ø30<br />
M25~0.75<br />
Plan<br />
50 X /0.90 oil<br />
/- WD 0.40<br />
ø5.5<br />
ø14<br />
ø18.8<br />
60.06 (NCG)<br />
CFI Plan Achromat 50x Oil<br />
ø30<br />
M25~0.75<br />
2.4 37<br />
10 5<br />
ø31<br />
CFI Plan Achromat UW 1x<br />
45˚<br />
45˚<br />
ø27<br />
M25~0.75<br />
Plan<br />
10X /0.25<br />
/-WD 10.5<br />
ø12<br />
ø19<br />
ø25<br />
60.06<br />
CFI Plan Achromat 10x<br />
CFI Plan Achromat DL 10x<br />
4.6 5 4.5<br />
34.5<br />
10 4.5<br />
ø20<br />
CFI Plan Achromat UW 2x<br />
45˚<br />
45˚<br />
ø27<br />
M25~0.75<br />
Plan<br />
20 X /0.40<br />
/ 0.17 WD 1.3<br />
ø7<br />
ø13<br />
ø17<br />
ø20<br />
ø25<br />
60.06<br />
CFI Plan Achromat 20x<br />
CFI Plan Achromat DL 20x<br />
3.3 4 7.5<br />
34.5<br />
10 5<br />
ø25<br />
CFI Plan Achromat 4x<br />
45˚<br />
30˚<br />
ø27<br />
M25~0.75<br />
Plan<br />
40 X /0.65<br />
/ 0.17<br />
ø6<br />
ø13<br />
ø17<br />
ø20<br />
ø25<br />
WD0.57<br />
60.06<br />
CFI Plan Achromat 40x<br />
CFI Plan Achromat DL 40x<br />
2.2 4 9 44.5<br />
5<br />
45˚<br />
22.5˚<br />
Plan<br />
100 X /1.25<br />
/ 0.17 WD 0.17<br />
ø5.3<br />
ø13<br />
ø16.8<br />
ø20<br />
ø25<br />
60.06<br />
CFI Plan Achromat 100x Oil<br />
CFI Plan Achromat DL 100x Oil<br />
5<br />
51.2<br />
2.2 6.3<br />
2<br />
45˚<br />
20˚<br />
Plan<br />
100 X /0.90 N<br />
/ 0 WD 0.26<br />
ø5.5<br />
ø14<br />
ø18.8<br />
60<br />
CFI Plan Achromat 100x NCG<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Ph<strong>as</strong>e ring<br />
CFI Plan Achromat UW 1x 0.04 3.2 200.0 171.88 180<br />
CFI Plan Achromat UW 2x 0.06 7.5 100.0 76.39 110<br />
CFI Plan Achromat 4x 0.10 30.0 50.0 27.50 65<br />
CFI Plan Achromat 10x 0.25 10.5 20.0 4.40 85<br />
CFI Plan Achromat DL 10x 0.25 10.5 20.0 4.40 85 ph1<br />
CFI Plan Achromat 20x 0.40 1.3 20.0 1.72 85<br />
CFI Plan Achromat DL 20x 0.40 1.3 20.0 1.72 85 ph1<br />
CFI Plan Achromat 40x 0.65 0.57 5.0 0.65 115 Spring-loaded<br />
CFI Plan Achromat DL 40x 0.65 0.57 5.0 0.65 115 Spring-loaded ph2<br />
CFI Plan Achromat 40x NCG 0.65 0.48 5.0 0.65 160 Spring-loaded No cover gr<strong>as</strong>s<br />
CFI Plan Achromat 50x Oil 0.90 0.4 4.0 0.52 160 Spring-loaded<br />
CFI Plan Achromat 100x Oil 1.25 0.17 3.3 0.52 160 Spring-loaded<br />
CFI Plan Achromat DL 100x Oil 1.25 0.17 3.3 0.52 160 Spring-loaded ph3<br />
CFI Plan Achromat 100x NCG 0.90 0.26 2.0 0.18 160 Spring-loaded No cover gr<strong>as</strong>s<br />
6<br />
7
CFI60 OBJECTIVES<br />
CFI Plan Fluor Series for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DLL)<br />
Featuring an extra-high transmission<br />
rate, especially in the ultraviolet<br />
wavelength, and flatness of field<br />
comparable to the CFI Plan Achromat<br />
series, these objectives are designed<br />
for fluorescence observation and<br />
photomicrography. Because of this<br />
improvement in quality, these<br />
objectives can function <strong>as</strong> multipurpose<br />
objectives for brightfield,<br />
fluorescence, polarizing, and DIC<br />
observations.<br />
The DLL (low contr<strong>as</strong>t) ph<strong>as</strong>e<br />
objectives for this series can also be<br />
used <strong>as</strong> universal objectives for<br />
ph<strong>as</strong>e contr<strong>as</strong>t, fluorescence, DIC,<br />
and brightfield techniques.<br />
5.3<br />
42.8<br />
ø30<br />
M25~0.75<br />
Plan Fluor<br />
4X / 0.13<br />
/ - WD 17.1<br />
ø20<br />
CFI Plan Fluor 4x<br />
45˚<br />
5.3<br />
43.9<br />
ø30<br />
M25~0.75<br />
Plan Fluor<br />
10X / 0.30<br />
DIC L@<br />
/0.17 WD 16.0<br />
ø18<br />
CFI Plan Fluor 10x<br />
CFI Plan Fluor DLL 10x<br />
45˚<br />
1.7<br />
5.3<br />
10.8 11<br />
32<br />
3.9<br />
ø34.5<br />
ø30<br />
M25~0.75<br />
Plan Fluor<br />
60X / 0.85<br />
DIC M<br />
/0.11-0.23 WD 0.30<br />
11 14 17 20 23<br />
ø8.5<br />
ø18<br />
ø22<br />
ø33<br />
30˚<br />
30˚<br />
5.3<br />
3.8 4.5<br />
51.5<br />
ø30<br />
Plan Fluor<br />
100X/1.30 oil<br />
DIC H<br />
/0.17 WD 0.20<br />
ø5.8<br />
ø17<br />
ø21<br />
20˚<br />
45˚<br />
6.2<br />
2.6<br />
5.3<br />
34<br />
2.9 6.5 7.5<br />
17˚<br />
ø33.4<br />
ø32<br />
ø30<br />
M25~0.75<br />
Plan Fluor<br />
100X/0.5-1.3 oil<br />
/0.17 WD 0.2<br />
1.3 0.7<br />
ø5.3<br />
ø19.8<br />
ø23.6<br />
ø32<br />
C2.5<br />
8˚<br />
ø30<br />
M25~0.75<br />
ø30<br />
M25~0.75<br />
ø33.5<br />
ø30<br />
CFI Plan Fluor 60x C<br />
CFI Plan Fluor 100x Oil<br />
CFI Plan Fluor DLL 100x Oil<br />
CFI Plan Fluor 100x Oil with<br />
iris diaphragm<br />
5.3<br />
54<br />
Plan Fluor<br />
20X / 0.50<br />
DIC M<br />
/0.17 WD 2.1<br />
45˚<br />
5.3<br />
57.2<br />
5.5<br />
Plan Fluor<br />
40X / 0.75<br />
DIC M<br />
/0.17 WD 0.72<br />
40<br />
12 5.3<br />
45˚<br />
60.41<br />
Plan Fluor<br />
4X / 0.13<br />
PhL DL<br />
/1.2 WD 16.4<br />
45˚<br />
5<br />
42.8<br />
5.3<br />
M25~0.75<br />
Plan Fluor<br />
40X / 1.30 oil<br />
DIC H<br />
/0.17 WD 0.2<br />
CFI Plan Fluor Series for Ph<strong>as</strong>e Contr<strong>as</strong>t (DL)<br />
These objectives have more contr<strong>as</strong>t than the DLL objectives for enhanced contr<strong>as</strong>t with tissue culture<br />
specimens. These objectives are designed for use on inverted microscopes and therefore their markings are<br />
upside-down for e<strong>as</strong>y reading.<br />
1.2<br />
45˚<br />
3.8<br />
2.7<br />
ø12<br />
ø17<br />
CFI Plan Fluor 20x<br />
CFI Plan Fluor DLL 20x<br />
2<br />
ø10<br />
45˚<br />
CFI Plan Fluor 40x<br />
CFI Plan Fluor DLL 40x<br />
4<br />
3.7<br />
23˚<br />
ø8<br />
ø22<br />
ø25.8<br />
CFI Plan Fluor 40x Oil<br />
60.41<br />
Plan Fluor<br />
10X / 0.30<br />
Ph1 DL<br />
/1.2 WD 15.2<br />
45˚<br />
6<br />
44<br />
5.3<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Ph<strong>as</strong>e ring<br />
CFI Plan Fluor 4x 0.13 17.1 50.0 16.27 110<br />
CFI Plan Fluor 10x 0.30 16.0 20.0 3.06 120<br />
CFI Plan Fluor DLL 10x 0.30 16.0 20.0 3.06 120 ph1<br />
CFI Plan Fluor 20x 0.50 2.1 10.0 1.10 175<br />
CFI Plan Fluor DLL 20x 0.50 2.1 10.0 1.10 175 ph1<br />
CFI Plan Fluor 40x 0.75 0.72 5.0 0.49 210 Spring-loaded<br />
CFI Plan Fluor DLL 40x 0.75 0.72 5.0 0.49 210 Spring-loaded ph2<br />
CFI Plan Fluor 40x Oil 1.30 0.2 5.0 0.25 230 Spring-loaded Stopper<br />
CFI Plan Fluor 60x C 0.85 0.3 3.3 0.38 265 Spring-loaded Cover gl<strong>as</strong>s correction 0.11–0.23mm<br />
CFI Plan Fluor 100x Oil 1.30 0.2 2.0 0.25 250 Spring-loaded Stopper<br />
CFI Plan Fluor DLL 100x Oil 1.30 0.2 2.0 0.25 250 Spring-loaded Stopper ph3<br />
CFI Plan Fluor 100x Oil with iris diaphragm 0.5–1.3 0.2 2.0 0.25 285 Spring-loaded With iris diaphragm<br />
M25~0.75<br />
M25~0.75<br />
ø30<br />
ø30<br />
CFI Plan Fluor DL 4x<br />
CFI Plan Fluor DL 10x<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Ph<strong>as</strong>e ring<br />
CFI Plan Fluor DL 4x 0.13 16.4 50.0 16.27 105 Cover gl<strong>as</strong>s thickness 1.2mm phL<br />
CFI Plan Fluor DL 10x 0.30 15.2 20.0 3.06 120 Cover gl<strong>as</strong>s thickness 1.2mm ph1<br />
8<br />
9
CFI60 OBJECTIVES<br />
CFI Plan Fluor ELWD Series for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DM/DLL)<br />
Offering superb flatness of field, high UV transmission rates, and high Numerical Apertures with extra long working distances, these objectives are<br />
well suited for fluorescence observations, especially with inverted microscopes. Because of their superior optical design, CFI Plan Fluor ELWD DM<br />
objectives can be used universally for all other observation techniques, including brightfield, fluorescence, ph<strong>as</strong>e contr<strong>as</strong>t, and Nomarski DIC.<br />
The DM ph<strong>as</strong>e contr<strong>as</strong>t objectives have higher contr<strong>as</strong>t to enhance the image of very thin, highly detailed specimens with low ph<strong>as</strong>e gradients.<br />
The DLL objectives have less contr<strong>as</strong>t for thicker, more contr<strong>as</strong>ty specimens.<br />
1.2<br />
Standard<br />
thickness<br />
ø32.5<br />
ø28.7<br />
ø25.5<br />
ø16.4<br />
ø32.5<br />
ø29<br />
ø27.5<br />
ø13.3<br />
ø32.5<br />
ø26.3<br />
ø10<br />
CFI Plan Apochromat Series for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DM)<br />
These objectives feature longer working distances with high Numerical Apertures and is designed to correct all<br />
optical aberrations throughout the visible spectrum from violet to red from center to edges across the entire 25mm<br />
field of view. Superior image flatness and color reproduction, plus resolving power at the theoretical limit of today's<br />
optical technology are also featured.<br />
The DM ph<strong>as</strong>e contr<strong>as</strong>t Plan Apochromats have higher contr<strong>as</strong>t to enhance images of very thin, highly detailed<br />
specimens with low ph<strong>as</strong>e gradients.<br />
60.41<br />
Plan Fluor<br />
LWD 20X/0.45<br />
/ 0-2 WD 7.4<br />
25˚<br />
5˚<br />
22.2 1.8<br />
1.8<br />
60.41<br />
Plan Fluor<br />
LWD 40X/0.60<br />
/0-2 WD 3.7 - 2.7<br />
20˚<br />
5<br />
26.5 2.5<br />
2.5<br />
60.41<br />
Plan Fluor<br />
WD 60X/0.70<br />
0.5-1.5 WD 2.1-1.5<br />
22.5<br />
7.5˚<br />
33<br />
5.3<br />
ø30<br />
M25~0.75<br />
5.3<br />
ø30<br />
M25~0.75<br />
5.3<br />
ø30<br />
M25~0.75<br />
2 1.6 1.2 0.8 0.4 0<br />
ø29<br />
ø32<br />
ø34<br />
7˚<br />
11.5<br />
13<br />
5<br />
CFI Plan Fluor ELWD 20x C<br />
CFI Plan Fluor ELWD DM 20x C<br />
2 1.6 1.2 0.8 0.4 0<br />
M25~0.75<br />
ø29<br />
ø32<br />
ø34<br />
7˚<br />
11.5<br />
13<br />
5<br />
CFI Plan Fluor ELWD 40x C<br />
CFI Plan Fluor ELWD DM 40x C<br />
1.5 1.2 1 0.8 0.5<br />
M25~0.75<br />
ø29<br />
ø33<br />
ø34<br />
9˚<br />
6<br />
11.5<br />
13<br />
5<br />
CFI Plan Fluor ELWD 60x C<br />
CFI Plan Fluor ELWD DLL 60x C<br />
51.4<br />
45˚<br />
Plan Apo<br />
2X / 0.1<br />
/ - WD 8.5<br />
ø19<br />
WD=8.5<br />
CG=0.17<br />
60.06<br />
44.2<br />
30<br />
Plan Apo<br />
4X / 0.2<br />
/ - WD 15.7<br />
ø18<br />
WD=15.7<br />
CG=0.17<br />
60.06<br />
55.9<br />
45˚<br />
Plan Apo<br />
10X / 0.45<br />
DIC L<br />
/0.17 WD 4.0<br />
ø15<br />
WD=4.0<br />
CG=0.17<br />
60.06<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Ph<strong>as</strong>e ring<br />
CFI Plan Fluor ELWD 20x C 0.45 8.1–7.0 10.0 1.36 195 Cover gl<strong>as</strong>s correction 0–2mm<br />
CFI Plan Fluor ELWD DM 20x C 0.45 8.1–7.0 10.0 1.36 195 Cover gl<strong>as</strong>s correction 0–2mm ph1<br />
CFI Plan Fluor ELWD 40x C 0.60 3.7–2.7 5.0 0.76 195 Cover gl<strong>as</strong>s correction 0–2mm<br />
CFI Plan Fluor ELWD DM 40x C 0.60 3.7–2.7 5.0 0.76 195 Cover gl<strong>as</strong>s correction 0–2mm ph2<br />
CFI Plan Fluor ELWD 60x C 0.70 2.1–1.5 3.3 0.56 210 Cover gl<strong>as</strong>s correction 0.5–1.5mm<br />
CFI Plan Fluor ELWD DLL 60x C 0.70 2.1–1.5 3.3 0.56 210 Cover gl<strong>as</strong>s correction 0.5–1.5mm ph2<br />
CFI Plan Apochromat 2x<br />
CFI Plan Apochromat 4x<br />
CFI Plan Apochromat 10x<br />
10<br />
Multi-Immersion Objective for Brightfield<br />
This multi-purpose immersion objective h<strong>as</strong> a higher numerical aperture and<br />
superior contr<strong>as</strong>t. The use of immersion media helps eliminate surface<br />
reflections and provides an image with higher contr<strong>as</strong>t. It is an excellent<br />
objective for fluorescence, brightfield, and DIC observation techniques.<br />
This unique objective h<strong>as</strong> a correction collar that allows it to be used with<br />
immersion oil, glycerin, and water (with and without a cover gl<strong>as</strong>s).<br />
Objective N.A. W.D. Focal length Physical depth Weight Remarks<br />
(magnification) (mm) (mm) of focus (µm) (g)<br />
CFI Plan Fluor 20x MI 0.75 0.35–0.33 10.0 0.49 300<br />
Multi-immersion<br />
Oil-glycerine-water<br />
10.4 19 12.5 14 5.3<br />
3.7<br />
Oil G<br />
ø36<br />
ø34.5<br />
ø30.6<br />
M25~0.75<br />
Plan Fluor<br />
20X / 0.75 Mlmm<br />
/ - WD 0.35<br />
ø8<br />
ø28<br />
ø33<br />
0.17 W<br />
0<br />
CFI Plan Fluor 20x MI<br />
40˚<br />
8˚<br />
3˚<br />
30˚<br />
5.3<br />
12<br />
5.9 2.5 38.5<br />
45˚<br />
30˚<br />
ø33.5<br />
ø30<br />
M25~0.75<br />
Plan Apo<br />
20X / 0.75<br />
DIC M<br />
/0.17 WD 1.0<br />
ø13.5<br />
ø22<br />
ø26<br />
WD=1.0<br />
CG=0.17<br />
60.06<br />
CFI Plan Apochromat 20x<br />
CFI Plan Apochromat DM 20x<br />
1.7<br />
4.2 10.8 11<br />
32 5.3<br />
30˚<br />
30˚<br />
3˚<br />
ø34.5<br />
ø30<br />
M25~0.75<br />
Plan Apo<br />
40X / 0.95<br />
DIC M<br />
/0.11-0.23 WD 0.14<br />
11 14 17 20 23<br />
ø7<br />
ø18<br />
ø22<br />
ø33<br />
WD=0.14<br />
CG=0.17<br />
60.06<br />
CFI Plan Apochromat 40x C<br />
CFI Plan Apochromat DM 40x C<br />
5<br />
12<br />
4.7 4.5 38.5<br />
45˚<br />
30˚<br />
5˚<br />
ø32<br />
ø30<br />
ø25<br />
Plan Apo<br />
40X / 1.0 oil<br />
DIC H<br />
/0.17 WD 0.16<br />
ø8<br />
ø18.8<br />
ø22.8<br />
WD=0.16 (oil)<br />
CG=0.17<br />
60.06<br />
CFI Plan Apochromat 40x Oil<br />
CFI Plan Apochromat DM 40x Oil<br />
11
CFI60 OBJECTIVES<br />
1.7<br />
5.3<br />
32<br />
11<br />
10.8<br />
4.2<br />
30˚<br />
30˚<br />
3˚<br />
ø34.5<br />
ø30<br />
M25~0.75<br />
Plan Apo<br />
60X /0.95<br />
DIC M<br />
/ 0.11-0.23 WD 0.15<br />
11 14 17 20 23<br />
ø7<br />
ø18<br />
ø22<br />
ø33<br />
WD=0.15<br />
CG=0.17<br />
60.06<br />
CFI Plan Apochromat 60x C<br />
CFI Plan Apochromat DM 60x C<br />
ø30<br />
M25~0.75<br />
CFI Plan Apochromat Series for Brightfield/Ph<strong>as</strong>e Contr<strong>as</strong>t (DM)<br />
3.7 4<br />
40<br />
12 5.3<br />
45˚<br />
22.5˚<br />
8˚<br />
ø33.5<br />
ø30<br />
M25~0.75<br />
Plan Apo<br />
60X /1.40 oil<br />
DIC H<br />
/ 0.17 WD 0.21<br />
ø7.2<br />
ø22<br />
ø25.8<br />
CG=0.17 WD=0.21 (oil)<br />
60.06<br />
CFI Plan Apochromat 60x Oil<br />
CFI Plan Apochromat DM 60x Oil<br />
ø30<br />
M25~0.75<br />
59.7 5.3<br />
23.5<br />
11<br />
11.9<br />
10.4<br />
2.9<br />
30˚<br />
7˚<br />
5˚<br />
30˚<br />
ø36.5<br />
ø35.2<br />
ø30<br />
M25~0.75<br />
Plan Apo<br />
60X /1.20 WI<br />
/ 0.15-0.18 WD 0.22<br />
15 16 17 18<br />
ø7.5<br />
ø29<br />
ø33.4<br />
CFI Plan Apochromat 60x WI<br />
CFI S Fluor Series<br />
These objectives ensure a high transmission rate of ultraviolet wavelengths down to 340 nm for fluorochromes like<br />
indo-1, fura-2, and fluor-3. Also, these objectives have improved signal-to-noise ratios (S/N) for short wavelengths and<br />
have high N.A., making the fluorescence images they produce significantly sharper and brighter.<br />
64<br />
5.3<br />
12<br />
38.5<br />
8.2<br />
ø32<br />
ø30<br />
M25~0.75<br />
S Fluor<br />
10 X / 0.50<br />
/ 0.17 WD 0.12<br />
ø8<br />
ø19<br />
ø24<br />
1.2<br />
64.2<br />
5.3<br />
5.9 41<br />
12<br />
ø33.5<br />
ø30<br />
M25~0.75<br />
S Fluor<br />
20 X / 0.75<br />
/ 0.17 WD 1.0<br />
ø9<br />
ø22<br />
ø25<br />
1<br />
5<br />
27<br />
3.1 8.5 10.5 10.5<br />
ø34.5<br />
ø33<br />
ø30<br />
M25~0.75<br />
Fluor 40 X / 0.9<br />
/ 0.11-0.23 WD 0.3<br />
11 14 17 20 23<br />
ø7.5<br />
ø18<br />
ø26<br />
ø33<br />
CFI S Fluor 10x<br />
CFI S Fluor 20x<br />
CFI S Fluor 40x C<br />
5.3<br />
59.8<br />
51.5<br />
Plan Apo<br />
100 X /1.40 oil<br />
DIC H<br />
/ 0.17 WD 0.13<br />
60.06<br />
59.8 5.3<br />
51.5<br />
Plan Apo<br />
00X /1.40 oilN<br />
/ 0 WD 0.17<br />
60<br />
5<br />
12<br />
ø32<br />
ø30<br />
M25~0.75<br />
5<br />
ø32<br />
ø30<br />
M25~0.75<br />
4.5<br />
3.8<br />
20˚<br />
C2<br />
ø5.8<br />
ø17<br />
ø21<br />
WD=0.13<br />
4.5<br />
3.8<br />
C2<br />
20˚<br />
ø5.8<br />
ø17<br />
ø21<br />
WD=0.17<br />
37<br />
S Fluor<br />
40 X / 1.30<br />
/ 0.17 WD 0.22<br />
Oil<br />
64.7<br />
32<br />
7.5<br />
7.5<br />
S Fluor<br />
100 X /0.5-1.3 oil<br />
/ 0.17 WD 0.2<br />
1.3 0.7<br />
CFI Plan Apochromat 100x Oil<br />
CFI Plan Apochromat DM 100x Oil<br />
CFI Plan Apochromat 100x NCG Oil<br />
6.5<br />
9.8<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Ph<strong>as</strong>e ring<br />
CFI Plan Apochromat 2x 0.10 8.5 100.0 27.50 180<br />
CFI Plan Apochromat 4x 0.20 15.7 50.0 6.88 180<br />
CFI Plan Apochromat 10x 0.45 4.0 20.0 1.36 220<br />
CFI Plan Apochromat 20x 0.75 1.0 10.0 0.49 240 Spring-loaded<br />
CFI Plan Apochromat DM 20x 0.75 1.0 10.0 0.49 240 Spring-loaded ph2<br />
CFI Plan Apochromat 40x C 0.95 0.14 5.0 0.30 270 Spring-loaded Cover gl<strong>as</strong>s correction 0.11–0.23mm<br />
CFI Plan Apochromat DM 40x C 0.95 0.14 5.0 0.30 270 Spring-loaded Cover gl<strong>as</strong>s correction 0.11–0.23mm ph2<br />
CFI Plan Apochromat 40x Oil 1.0 0.16 5.0 0.42 240 Spring-loaded Stopper<br />
CFI Plan Apochromat DM 40x Oil 1.0 0.16 5.0 0.42 240 Spring-loaded Stopper ph3<br />
CFI Plan Apochromat 60x C 0.95 0.15 3.3 0.30 285 Spring-loaded Cover gl<strong>as</strong>s correction 0.11–0.23mm<br />
CFI Plan Apochromat DM 60x C 0.95 0.15 3.3 0.30 285 Spring-loaded Cover gl<strong>as</strong>s correction 0.11–0.23mm ph2<br />
CFI Plan Apochromat 60x Oil 1.40 0.21 3.3 0.21 285 Spring-loaded Stopper<br />
CFI Plan Apochromat DM 60x Oil 1.40 0.21 3.3 0.21 285 Spring-loaded Stopper ph3<br />
CFI Plan Apochromat 60x WI 1.20 0.22 3.3 0.25 285 Spring-loaded Cover gl<strong>as</strong>s correction 0.15–0.18mm<br />
Water immersion<br />
CFI Plan Apochromat 100x Oil 1.40 0.13 2.0 0.21 245 Spring-loaded Stopper<br />
CFI Plan Apochromat DM 100x Oil 1.40 0.13 2.0 0.21 245 Spring-loaded Stopper ph3<br />
CFI Plan Apochromat 100x NCG Oil 1.40 0.17 2.0 0.21 245 Spring-loaded Stopper No cover gl<strong>as</strong>s<br />
4.2<br />
ø8.3<br />
ø20<br />
ø23.8<br />
CFI S Fluor 40x Oil<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks<br />
CFI S Fluor 10x 0.50 1.2 10.0 1.10 275 Spring-loaded<br />
CFI S Fluor 20x 0.75 1.0 10.0 0.49 275 Spring-loaded<br />
CFI S Fluor 40x C 0.90 0.3 5.0 0.34 285 Spring-loaded Cover gl<strong>as</strong>s correction 0.11–0.23mm<br />
CFI S Fluor 40x Oil 1.30 0.22 5.0 0.25 275 Spring-loaded<br />
CFI S Fluor 100x Oil 1.30 0.2 2.0 0.25 285 Spring-loaded<br />
2.9<br />
ø6<br />
ø17<br />
ø24<br />
ø29.5<br />
CFI S Fluor 100x Oil<br />
12<br />
13
CFI60 OBJECTIVES<br />
Water Dipping Series CFI HMC ® Series for Hoffmann Modulation Contr<strong>as</strong>t ®<br />
These objectives (no cover gl<strong>as</strong>s) are designed with a narrow top and a 2mm working distance to allow<br />
microelectrodes to be inserted into the specimen at 45°. They use ceramic and gl<strong>as</strong>s nose cones to isolate them<br />
thermally and electronically. Since they are FLUOR lenses, they are designed for fluorescence and have very high<br />
transmission in the UV and IR wavelengths with a high signal-to-noise ratio for superior images.<br />
ø29<br />
M25~0.75<br />
ø29<br />
M25~0.75<br />
ø29<br />
M25~0.75<br />
These objectives have been completely redesigned by <strong>Nikon</strong>, allowing the contr<strong>as</strong>t direction to be changed using<br />
a modulator inside the objective. That direction, once set, is maintained over the entire magnification range from<br />
10X to 40X.<br />
Note: Hoffman Modulation Contr<strong>as</strong>t and HMC are registered trademarks of Modulation Optics Inc.<br />
ø25<br />
ø13.3<br />
ø10<br />
ø25<br />
ø16.5<br />
ø15<br />
ø8<br />
ø33.3<br />
ø32<br />
ø28.8<br />
ø25.5<br />
ø20<br />
ø12<br />
5<br />
58.5<br />
32.7<br />
1.5 12 3.8 8.5<br />
55˚<br />
45˚<br />
Fluor<br />
10X / 0.30 W<br />
DIC L<br />
/ 0 WD 2.0<br />
ø3.5<br />
ø5<br />
ø20.5<br />
ø24<br />
ø26<br />
48˚<br />
2 (Water dipping)<br />
60.5<br />
5<br />
58.5<br />
32.7<br />
4.5 5 5 11.3<br />
50˚<br />
45˚<br />
Fluor<br />
40X / 0.80 W<br />
DIC M<br />
/ 0 WD 2.0<br />
ø4<br />
ø11.5<br />
ø19<br />
ø23<br />
ø25.5<br />
45˚<br />
2 (Water dipping)<br />
60.5<br />
5<br />
58.5<br />
32.7<br />
1 5.5 8 11.3<br />
40˚<br />
30˚<br />
Fluor<br />
60X / 1.00 W<br />
DIC H<br />
/ 0 WD 2.0<br />
ø5.5<br />
ø19.2<br />
ø23<br />
ø25.5<br />
33.7˚<br />
2 (Water dipping)<br />
60.5<br />
60.41<br />
45˚<br />
45˚<br />
10X / 0.25<br />
/ 1.2 WD 5.2<br />
MC1 HMC<br />
M25~0.75<br />
ø27<br />
ø27.8<br />
4.5 10 13<br />
28.5 4.5 2.5<br />
60.41<br />
45˚<br />
45˚<br />
D 20X / 0.4<br />
/ 1.2 WD 3.0<br />
MC2 HMC<br />
M25~0.75<br />
ø27<br />
ø27.8<br />
4.8<br />
10 13<br />
28.5<br />
4.5<br />
60.41<br />
WD 40X / 0.55<br />
/0- 2 WD 2.7-1.7<br />
2<br />
MC3 HMC<br />
1.6 1.2 0.8 0.4<br />
M25~0.75<br />
ø29<br />
ø32<br />
ø34<br />
0<br />
35˚<br />
1<br />
5 13 11.5 8 19 5.6<br />
CFI Fluor 10x W<br />
CFI Fluor 40x W<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks<br />
CFI Fluor 10x W 0.30 2.0 20.0 4.06 210 Water dipping<br />
CFI Fluor 40x W 0.80 2.0 5.0 0.57 210 Water dipping<br />
CFI Fluor 60x W 1.0 2.0 3.3 0.37 210 Water dipping<br />
CFI Fluor 60x W<br />
CFI HMC 10x<br />
2nd Objective Lens Unit<br />
CFI HMC 20x F<br />
Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks<br />
CFI HMC 10x 0.25 5.2 20.0 4.40 130 Cover gl<strong>as</strong>s thickness 1.2mm<br />
CFI HMC 20x F 0.40 3.0 10.0 1.72 130 Cover gl<strong>as</strong>s thickness 1.2mm<br />
CFI HMC LWD 40x C 0.55 2.7–1.7 5.0 0.91 210 Cover gl<strong>as</strong>s correction0–2mm<br />
This unit is attached to infinity objectives when neither a<br />
binocular or trinocular eyepiece tube is used.<br />
• Infinity Tube Lens Unit for CFI objectives (chromatic aberrationfree<br />
infinity corrected objectives).<br />
• Focusing distance: 200mm (7.87 in.)<br />
• To obtain the optimal objective performance, keep the distance<br />
between the tube lens and the objective’s shoulder within<br />
100–200 mm (3.84–7.87 in.).<br />
28<br />
22.5<br />
M38~0.5<br />
5<br />
ø36f7 -0.025<br />
-0.050<br />
ø33<br />
CFI HMC LWD 40x C<br />
Approx. 70~170 148<br />
Image plane<br />
Objective's shoulder<br />
Plan Fluor<br />
100X / 1.30 oil<br />
DIC H<br />
/ 0.17 WD 0.20<br />
14<br />
2nd Objective Lens Unit<br />
15
CFI60 OBJECTIVES<br />
Markings on CFI60 Objectives<br />
Condensers<br />
2.5 58<br />
21<br />
ø27<br />
ø8<br />
30˚<br />
ø50<br />
14.5 6<br />
7.5<br />
(1) Magnification and Color Code<br />
A color coded ring on the barrel identifies<br />
the magnification of the objective:<br />
(2) Numerical Aperture (N.A.)<br />
N.A. is the most important factor in defining<br />
the performance characteristics of an objective.<br />
N. A.= n sin <br />
n: the refractive index of the media at d-line (587nm)<br />
For dry objective n=1.000 (air)<br />
For oil objective n=1.515 (oil)<br />
For water objective n= 1.333 (water)<br />
:Half angle of incident rays to the top lens of the objective<br />
The higher N.A., the higher resolving power.<br />
When the resolving power is defined <strong>as</strong> the power to<br />
recognize the two points,<br />
R=<br />
or<br />
(1) Magnification (2) Numerical Aperture<br />
(6) Application Markings<br />
<br />
N.A.<br />
R=<br />
<br />
N.A. Obj. +N.A. Cond.<br />
The higher N.A. the brighter image we take.<br />
2<br />
Brightness: B <br />
N.A.<br />
Total Magnification <br />
The higher N.A., the shallower the depth of focus (DOF).<br />
DOF = N.A. 2<br />
(1) Color Code<br />
(7) Immersion Oil—cleaning<br />
Mag. 1X 2X 4X 10X 20X 40X 50X 60X 100X<br />
Color code Black Gray Red Yellow Green<br />
Light<br />
Blue<br />
Light<br />
Blue<br />
Cobalt<br />
Blue<br />
White<br />
(3) Working Distance<br />
Working distance (W.D.) defines the distance between<br />
the top lens of the objective and the surface of the<br />
cover gl<strong>as</strong>s.<br />
CFI60 objectives can offer longer working distance with<br />
high numerical aperture.<br />
(3) Working Distance<br />
<strong>Nikon</strong> offers a wide variety of CFI objectives. To <strong>as</strong>sist the user they are clearly marked with information<br />
on the objective barrel such <strong>as</strong>: which DIC module or Ph<strong>as</strong>e Ring to use.<br />
n = 1.515<br />
Oil<br />
n = 1.000<br />
Dry<br />
Objective lens<br />
<br />
Cover Gl<strong>as</strong>s<br />
Slide Gl<strong>as</strong>s<br />
(4) Correction Ring<br />
Dry objectives with high Numerical Aperture are<br />
susceptible to spherical and other aberrations which<br />
can impair resolution and contr<strong>as</strong>t when used with a<br />
cover gl<strong>as</strong>s whose thickness differs from the specified<br />
value. A 1 1/2 cover gl<strong>as</strong>s (0.17mm thick) should be used<br />
<strong>as</strong> standard, however not all 11/2 cover gl<strong>as</strong>ses are<br />
exactly 0.17mm and many specimens have media<br />
between them and the cover gl<strong>as</strong>s. The correction ring<br />
is used to adjust for these subtle differences to ensure<br />
the optimum objective performance.<br />
How to use the correction ring<br />
Position the ring at 0.17. The thickness of the<br />
standard cover gl<strong>as</strong>s is 0.17mm.<br />
Focus the lens on a small artifact in the specimen.<br />
Rotate the ring very slightly and focus the lens again<br />
to check if the image h<strong>as</strong> improved or degraded.<br />
Repeat the above step to determine if the image is<br />
improving or degrading in the direction you are<br />
turning the ring.<br />
If the image h<strong>as</strong> degraded, follow the same<br />
procedure in the opposite direction to find the<br />
position offering optimum resolving power and<br />
contr<strong>as</strong>t.<br />
(5) Cover Gl<strong>as</strong>s<br />
For optimum performance, the thickness of the cover<br />
gl<strong>as</strong>s should be 0.17mm. For example, at N.A.=0.95, a<br />
0.01mm difference in thickness reduces image<br />
formation by 45% from the ideal image.<br />
N.A.<br />
0.3<br />
0.45<br />
0.7<br />
0.85<br />
0.95<br />
Difference in cover gl<strong>as</strong>s thickness<br />
0.01mm<br />
0.02mm<br />
100%<br />
100<br />
98<br />
81<br />
45<br />
(4) Correction Ring<br />
(5) Cover Gl<strong>as</strong>s<br />
100%<br />
100<br />
92<br />
43<br />
29<br />
(6) Application Markings<br />
DIC: for Differential Interference Contr<strong>as</strong>t<br />
DM: Ph<strong>as</strong>e contr<strong>as</strong>t, Dark contr<strong>as</strong>t middle type<br />
DL: Ph<strong>as</strong>e contr<strong>as</strong>t, Dark contr<strong>as</strong>t light type<br />
DLL: Ph<strong>as</strong>e contr<strong>as</strong>t, Lower contr<strong>as</strong>t type<br />
(7) Immersion Oil—cleaning<br />
After using immersion oil, gently blot the lens dry with<br />
lens tissue. Then slightly moisten a piece of lens tissue<br />
with petroleum benzene (Naphtha) and clean off all<br />
traces of the oil from the immersion objective. Cleaning<br />
is essential for water immersion objectives <strong>as</strong> well; after<br />
use, wipe the water off the top lens.<br />
Type N.A. Object distance (mm) Magnification<br />
C-C Abbe Condenser, Dry Type 0.9 1.9 4–100x<br />
C-C Achromat Condenser 0.8 4.2 4–100x<br />
Achromat Swing-out Condenser 0.9/0.22 1.8 2–100x<br />
C-C Achromat /Aplanat Condenser 1.40 1.6 10–100x<br />
LWD Achromat Condenser 0.65 10.2 4–40x<br />
C-C Low Power Condenser 0.13 10.2 1–4x<br />
Dark Field Condenser, Oil 1.2–1.43 1.5 20–100x<br />
Dark Field Condenser, Dry 0.8–0.95 4.0 20–40x<br />
16 17<br />
1.0<br />
(Slidgl<strong>as</strong>s thickness)<br />
55<br />
10<br />
R40<br />
ø55<br />
ø43<br />
ø40<br />
ø54<br />
ø59<br />
0.7<br />
8 54.9<br />
55.6<br />
C-C Abbe Condenser, Dry Type<br />
8 55.5 1<br />
(Slidgl<strong>as</strong>s thickness)<br />
0.6<br />
(Oil)<br />
54.4 -0.2<br />
0<br />
70˚<br />
ø54<br />
Achr-ADl N.A=1.4 JAPAN<br />
1.4 1.2 1.0 0.8 0.6 0.4 0.2<br />
ø40<br />
ø62<br />
C-C Achromat /Aplanat Condenser<br />
55 0.5<br />
14 9.5 (Oil)<br />
20<br />
70˚<br />
ø39<br />
ø34.5<br />
Dark Field Condenser<br />
Oil 1.43-1.20<br />
JAPAN<br />
ø40<br />
ø56<br />
Dark Field Condenser, Oil<br />
R40<br />
ø55<br />
ø36.8<br />
ø40<br />
ø54<br />
ø58<br />
2.0<br />
8 54.5<br />
56.5<br />
C-C Achromat Condenser<br />
1<br />
55.6<br />
54<br />
8<br />
(Slidgl<strong>as</strong>s thickness)<br />
1.5<br />
ø59<br />
ø54.5<br />
ø42<br />
ø36.8<br />
ø22<br />
ø40<br />
9<br />
12<br />
24.5<br />
LWD Achromat Condenser<br />
70˚<br />
20.6<br />
ø35.5<br />
Dark Field Condenser<br />
Dry 0.95-0.80<br />
JAPAN<br />
ø40<br />
ø56<br />
Dark Field Condenser, Dry<br />
29.8<br />
4.5˚<br />
8.6<br />
8<br />
Achr 0.90 JAPAN<br />
0.8 0.6 0.4 0.2<br />
54.8<br />
63.4<br />
ø60<br />
ø10<br />
34.5<br />
19<br />
13<br />
11.5<br />
4.5˚<br />
R40<br />
ø40<br />
Achromat Swing-out Condenser<br />
R40<br />
ø55<br />
ø38<br />
ø40<br />
ø54<br />
ø58<br />
8 58.7<br />
8.3<br />
67<br />
C-C Low Power Condenser<br />
C-CEL<br />
ø30<br />
11.5<br />
14.9<br />
8<br />
C-CEL Expander Lens (for darkfield)<br />
55<br />
70˚
Illumination Systems<br />
139<br />
168.5<br />
139<br />
50<br />
12 24.5 13.5<br />
90˚<br />
76<br />
3.5<br />
28.5<br />
15<br />
76<br />
7<br />
4<br />
4.6<br />
30 8<br />
Eyepiece Tubes<br />
(Center of lamp)<br />
30<br />
6.6<br />
67.8<br />
20.5<br />
37.8<br />
i35.5‘39.5j<br />
7<br />
ø57<br />
41.5<br />
ø67<br />
ø51<br />
ø71<br />
M50~0.75<br />
ø71<br />
ø61<br />
ø55<br />
105<br />
24<br />
61 (Center of lamp)<br />
105<br />
24<br />
61<br />
(Center of lamp)<br />
20.5<br />
3<br />
4<br />
171<br />
1 170<br />
80<br />
80.5<br />
Lamphouse HMX-3B<br />
171<br />
1 170<br />
80<br />
80.5<br />
Lamphouse HMX-4B, built-in back mirror<br />
(Datum clamp face)<br />
(Datum clamp face)<br />
C-LA HMX Lamphouse Adapter<br />
(Datum clamp face)<br />
(Datum clamp face)<br />
HMX Connecting Adapter SB-X1<br />
(Bayonet to Screw)<br />
M50~0.75-6H<br />
Sectional View<br />
HMX Connecting Adapter SB-X2<br />
(Screw to Bayonet)<br />
ø42<br />
4<br />
60<br />
80<br />
64<br />
77 69.5<br />
100<br />
70<br />
105 (Min. distance)<br />
50<br />
64<br />
(Tilting section)<br />
98<br />
100<br />
110<br />
Approx. 111<br />
90.5<br />
8 63<br />
R32<br />
R40<br />
Y-TB Binocular Tube<br />
8˚ (Min. angle)<br />
ø51.3<br />
5<br />
70<br />
50<br />
50.5 77<br />
Y-TE Ergonomic Binocular Tube<br />
120<br />
2<br />
ø56<br />
47<br />
68<br />
80<br />
109<br />
67.8<br />
137.3<br />
99.5<br />
81.5<br />
52.5<br />
40<br />
70 8.5<br />
26<br />
102<br />
20<br />
138.5<br />
76.5<br />
6<br />
136<br />
34<br />
2<br />
51<br />
10<br />
148<br />
Lamphouse HMX<br />
Mercury Lamp Socket S 100W for HMX-3B<br />
27.1<br />
135.9<br />
50<br />
ø50<br />
ø52<br />
16.5<br />
ø58<br />
ø52<br />
172<br />
10.3 0.6<br />
24 5<br />
(Center of pin)<br />
(Center of filament)<br />
218.5<br />
151.5<br />
ø59<br />
16.5<br />
3<br />
Halogen Lamp Socket 100W/HMX<br />
58.5<br />
100<br />
73.7<br />
38.5<br />
7.5<br />
12<br />
36 11<br />
3<br />
36.5<br />
12.5<br />
66.5<br />
102.5<br />
i239j<br />
76.5<br />
18<br />
ø45<br />
1.1<br />
ø33<br />
Center <br />
of <br />
lamp<br />
3 22<br />
ø3<br />
6<br />
C-FC Epi-Fl Collector Lens<br />
ø45<br />
ø36<br />
Center <br />
of <br />
lamp<br />
3 21<br />
Quartz Epi-Fl Collector Lens<br />
ø45<br />
Center of<br />
lamp<br />
3 22<br />
Collector Lens/QH100W<br />
Y-TF Trinocular Tube “F” UW<br />
Y-TT Trinocular Tube “T” UW<br />
Y-TB Binocular Tube<br />
F.O.V. 22mm<br />
Y-TE Ergonomic Binocular Tube F.O.V. 22mm with tilting angle of 8° to 32° and telescopic mechanism of ±15mm movement<br />
Y-TF Trinocular Tube “F” UW Two-way: 100 : 0 or 0 : 100 (observation : photo) F.O.V. 22mm or 25mm with eyepiece reticule orientation adjustment mechanism<br />
Y-TT Trinocular Tube “T” UW Three-way: 100 : 0, 20 : 80, or 0 : 100 (observation : photo) F.O.V. 22mm or 25mm with eyepiece reticule orientation adjustment mechanism<br />
19
Intermediate Tubes<br />
Nosepieces<br />
22.5<br />
22.5<br />
(Top View)<br />
50.5<br />
285.5<br />
175<br />
V-ND JAPAN<br />
V-N JAPAN<br />
264<br />
248<br />
80<br />
100<br />
100<br />
80<br />
126.5<br />
13.5<br />
57.5<br />
22<br />
50<br />
123.2<br />
50<br />
123.2<br />
3<br />
(Right-side View)<br />
ø52<br />
6<br />
1<br />
2<br />
53<br />
48<br />
2<br />
53<br />
48<br />
120.6<br />
55<br />
34.5<br />
20.5<br />
322<br />
353.5<br />
45 25<br />
5<br />
55<br />
45<br />
100<br />
0<br />
Y-IDP Double Port<br />
ø51<br />
V-NA Motorized Sextuple Nosepiece<br />
ø121<br />
V-ND MG-DIC Intelligent Sextuple Nosepiece<br />
ø121<br />
Y-FL Epi-Fl Attachment<br />
Eyepieces<br />
22.5<br />
22.5<br />
48–56<br />
48–56<br />
22.5<br />
22.5<br />
C-ND DIC6<br />
JAPAN<br />
ø30<br />
CFI 10X / 22<br />
ø34<br />
ø38.8<br />
ø30<br />
CFI 10X / 22M<br />
ø34<br />
ø38.8<br />
50<br />
105.8<br />
50<br />
123.2<br />
CFI 10x with diopter adjustment<br />
CFI 10x M Photom<strong>as</strong>k with diopter adjustment<br />
22.5<br />
42–47<br />
22.5<br />
37–41<br />
ø30<br />
CFI 12.5X / 16<br />
ø35<br />
ø38.8<br />
ø30<br />
CFI 15X / 14.5<br />
ø35<br />
ø38.8<br />
53<br />
51.1<br />
48<br />
53<br />
48<br />
ø106<br />
ø121<br />
C-N Sextuple Nosepiece<br />
C-ND Sextuple DIC Nosepiece<br />
CFI 12.5x with diopter adjustment<br />
CFI 15x with diopter adjustment<br />
Type (magnification) F.O.V. Remarks<br />
CFI 10x with diopter adjustment<br />
22mm<br />
CFI 10x M Photom<strong>as</strong>k with diopter adjustment 22mm With photom<strong>as</strong>k reticule<br />
CFI 12.5x with diopter adjustment<br />
16mm<br />
CFI 15x with diopter adjustment<br />
14.5mm<br />
20<br />
21
CCTV Camera Adapters<br />
Focal point<br />
Focal point<br />
3<br />
72<br />
27<br />
ø52<br />
35<br />
ø12<br />
9.3<br />
ø51<br />
ø38<br />
76.5<br />
C-mount screw<br />
41<br />
25.5<br />
TV camera imaging plane<br />
ø50<br />
ø25.4<br />
ø38<br />
11.47 17.526<br />
30˚<br />
C-Mount TV Adapter A<br />
ø23.1<br />
ø42<br />
ø53<br />
17.526<br />
30.7 27.3 4<br />
100.1<br />
C-Mount TV Adapter 0.45x<br />
ø23.1<br />
ø42<br />
ø53<br />
17.526<br />
30.7 27 4<br />
116.8<br />
C-Mount TV Adapter 0.6x<br />
Focal point<br />
ø38<br />
ø52<br />
ø38<br />
ø59<br />
Focal point<br />
V-T Photo Adapter<br />
Y-T TV Tube for C-Mount Adapter A<br />
38<br />
48<br />
ø34<br />
ø30<br />
44<br />
31<br />
Relay Lens 1x I<br />
ø58.5<br />
ø54<br />
75<br />
10<br />
Image plane <br />
on eyepiece tube side<br />
ø31<br />
ø42<br />
ø46<br />
45˚<br />
7.5<br />
27.3<br />
64.5<br />
C-Mount TV Adapter<br />
91.8 17.526<br />
95.8<br />
ø42<br />
ø47<br />
ø62<br />
ø63<br />
27.8 11.5<br />
ENG-Mount TV Adapter<br />
53.3<br />
61.3}0.1 48<br />
64.3<br />
ø23.1<br />
ø42<br />
ø53<br />
30.2 27<br />
4<br />
111.2<br />
ENG-Mount TV Adapter 0.45x<br />
(for Trinocular Tube “F”, “T”)<br />
ø23.1<br />
ø42<br />
ø53<br />
30.2 27.0<br />
3.3<br />
122.8<br />
ENG-Mount TV Adapter 0.6x<br />
(for Trinocular Tube “F”, “T”)<br />
Focal point<br />
130<br />
67.3<br />
40.2<br />
JAPAN<br />
ø44<br />
ø54<br />
C-mount screw<br />
45˚<br />
11.5 4<br />
7.5 42.8 17.526<br />
54.3<br />
ø44<br />
ø57<br />
48<br />
12.3<br />
7.5<br />
22.8<br />
Focal point<br />
ø53<br />
ø38<br />
42 29<br />
68<br />
ø53<br />
29<br />
83<br />
109<br />
ø23.1<br />
C-Mount Zooming Adapter<br />
ENG-Mount Zooming Adapter<br />
C-Mount TV Adapter VM 2.5x<br />
ø42<br />
ø38<br />
Zoom Lens<br />
C-Mount TV Adapter VM 4x<br />
22<br />
23