Vol 27 No 2 December - The Indian Society for Parasitology

More documents

Recommendations

Info

114 Distribution of trace metals in Avitellina Lahorea and Serum of Sheep JPD : Vol. 27 (2), 2003 Tissue and Serum Electrolytes gravid region of parasite and host serum and the highest concentration of calcium in the gravid region was found to be significant. 100 mg of tissues from immature, mature and gravid proglottid regions were taken for the electrolyte analysis. The tissues were digested in 2 ml of TABLE I concentrated HC1 and diluted with distilled water and made up to 10 ml for the estimation. The above was filtered and the filtrate was used for the analysis of sodium, potassium and calcium by using Atomic Emission Spectrophotometer (THE VARIAN TEACHTRON AA6 6D) at the range of 589.592nm for + ++ ++ Na , 422.673nm for Ca and 766.49 Inm for K IIT, Madras). The host blood was collected in different test ± ± ± ± tubes without adding anticoagulant and brought to the S.E. 4.101 3.653 3.634 17.827 laboratory for the analysis of serum. One ml of serum was made up to 3 ml with double distilled water and the above extract was used for the electrolyte analysis. Determination of Cobalt, Selenium and Zinc 100 mg of immature, mature and gravid segments were digested with triple acid in the ratio of 10:3:l (concentrated nitric acid + 70% perchloric acid + concentrated sulphuric acid). Cobalt, Selenium and Zinc contents of the digests and 1 ml of serum with 2 ml double distilled water were determined using an Atomic Absorption Spectrophotometer (THE VARIAN TEACHTRON AA6 6D) with aqueous calibration standards. RESULTS The concentrations of electrolytes, sodium, potassium and calcium measured in the different strobilar regions of A.lahorea and host serum were given in the Table I and Figure I (A, B & C). Regarding sodium concentration, it was observed that mature region showed significantly higher value than the other regions. No significant difference was observed between the values of immature and gravid regions. Remarkably highest value of sodium concentration was noticed in host serum, than the strobilar regions of the parasite, which was found to be significant (P=0.01). The different strobilar regions viz., immature, mature and gravid of the parasite were found to contain lesser concentration of potassium than the host serum. This higher content of potassium in host serum was found to be statistically significant (P=0.01). The calcium content observed between Concentration of sodium, potassium and calcium ions, in the proglottides of adult cestode Avitellina lahorea and host (sheep) serum (ppm/µg/ml) Immature Mature Gravid Serum F-value P-value Sodium (a) (b) (a) (b) Mean 101.769 162.960 104.707 2412.00 14513.36 0.000** Potassium (a) (a) (a) (b) Mean 1.447 1.687 1.847 10.717 648.78 0.000** ± ± ± ± S.E. 0.016 0.022 0.020 0.354 Calcium (a) (a) (b) (b) Mean 14.736 17.758 25.501 22.642 28.42 0.000 ± ± ± ± S.E. 0.237 0.260 1.766 0.194 Each value represents mean ±SE of six observations ** denote significant at 1% level Concentration in order a
JPD : Vol. 27 (2), 2003 Distribution of trace metals in Avitellina Lahorea and Serum of Sheep 115 TABLE II A by the above symports viz., Na/glucose and Na/aminoacids (Thomborough, 1995). There is no Concentration of cobalt, selenium and zinc in the marked variation in the percent water content in the proglottides of adult cestode Avitellina lahorea and different regions of A.lahorea. The above finding also host (sheep) serum (ppm/µg/ml) suggests that the cell electrolyte Na may be involved in the cell volume regulation against the osmotic changes Immature Mature Gravid Serum F-value P-value in the luminal environment. The influx of Na along Cobalt (b) (a) (a) (a) with cations inside the segments may increase their Mean 0.029 0.011 0.007 0.015 16.02 0.000** osmolarity and prevent the water loss in case, fluidity ± ± ± ± of the lumen changes. S.E. 0.004 0.000 0.001 0.002 The electrolyte potassium level showed minimal Selenium (c) (b) (a) (d) Mean 0.379 0.280 0.188 0.526 106.19 0.000** concentrations in all the three different regions. Such ± ± ± ± hypokalemic condition confirms to the documented S.E. 0.017 0.017 0.006 0.014 concept that the living organisms will exhibit Zinc (c) (b) (a) (d) hypematremia and hypokalemia in the normal Mean 0.418 0.314 2.227 0.625 1760.03 0.000 physiological condition (von Brand, 1966). ± ± ± ± S.E. 0.004 0.005 0.004 0.002 The increasing gradient of calcium concentration towards the posterior segments agrees with the report Each value represents mean ± SE of six observations ** denote significant at 1% level of others (Chowdhury and DeRycke, 1977; Concentration in order a
Page 2 and 3: JOURNAL OF PARASITIC DISEASES (ISSN
Page 4 and 5: Journal of Parasitic Diseases Copyr
Page 6 and 7: SHORT COMMUNICATIONS On a new speci
Page 8 and 9: 70 Trematodes of public health impo
Page 14 and 15: Journal of Parasitic Diseases Vol.
Page 16 and 17: 78 Parasitic infections in traveler
Page 24 and 25: 86 CSF production induced by L. don
Page 34 and 35: 96 Humoral immune response to helmi
Page 36 and 37: 98 Humoral immune response to helmi
Page 38 and 39: 100 Red cell genetic defects and ma
Page 40 and 41: 102 Red cell genetic defects and ma
Page 44 and 45: 106 AMA1 and DBP polymorphism in P.
Page 46 and 47: H:\JPD PGI\December\Human infesting
Page 48 and 49: 110 Human Infesting Ixodid Ticks of
Page 50 and 51: 112 Human Infesting Ixodid Ticks of
Page 54 and 55: 116 Distribution of trace metals in
Page 56 and 57: 118 Distribution of trace metals in
Page 58 and 59: 120 Effect of Dextran and Polyvinyl
Page 60 and 61: 122 Effect of Dextran and Polyvinyl
Page 68 and 69: JPD : Vol. 27 (2), 2003 JOURNAL OF
Page 70 and 71: JPD : Vol. 27 (2), 2003 AUTHOR INDE
Page 72 and 73: JPD : Vol. 27 (2), 2003 CONGRATULAT
Page 74: JOURNAL OF PARASITIC DISEASES Volum

Vol 27 No 2 December - The Indian Society for Parasitology

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?