Vol 27 No 2 December - The Indian Society for Parasitology

JPD : Vol. 27 (2), 2003
AMA1 and DBP polymorphism in P.vivax
107
All these observations are based on sequence analysis
of this region (Tsuboi et al 1994, Ampudia et al 1996,
knowlesi 66 kDa merozoite antigen Parasite Immunol 70:535-552,
Kho et al 2001, Xalini et al 2000). In the present study
no variations in polymorphic region of DBP gene in erythrocytes by malaria merozoites. Science 182 :48 - 750,
clinical isolates collected from Northern region of
India by PCR-RFLP technique was observed.
Deans JD, Knight AM. Jeans WC, Waters AP, Cohen S and Mitchell GH.
1988. Vaccination trials in rhesus monkeys with minor, invariant P.
Dvorak J A. Miller LN, Whitehouse WC, Shiroishi T. 1975. Invasion of
Figtree M, Pasay CJ, Slade R, Cheng Q, Cloonan N, Walker J and Saul A.
2000. Plasmodium vivax synonymous substitution frequencies,
evolution and population structure deduced from diversity in AMA -1
and MSP-1 genes. Mol. Biochem. Parasitol 108. 53-66.
PCR or PCR-RFLP is not ideal method to study the
genetic variation. The strains which show no variation
Kho WG, Chung JY, Sim EJ, Kirn DW, Chung WC. 2001. Analysis of
polymorphic regions of Plasmodium vivax Duffy binding protein of
by these techniques may show small nucleotide Korean isolates. Korean J. Parasitol 59:143-150.
changes on the sequencing of the genes. Therefore, Laserson KF, Petraland, Hamlin D.M., Almera, R Fuentes M, Carrasquel
there is a need of further analysis of AMA-1 and DBP A and Barker (Jr) RH. 1994. Use of the polymerase chain reaction to
directly detect malaria parasites in blood samples from the
genes of P. vivax strains prevalent in Northern region
Venezuelan Amazon. Am J. Trop. Med. Hyg. 50:169-180.
of India by gene sequencing which is a powerful
Miller LH, Mason SJ, Clyde DZ and McGinnis MH. 1976.The resistance
technique to study small variations. However, the factor to P. vivax in blacks. The Duffy blood group genotype, Fy Fy. N.
functional constraints in both the proteins appear to Eng. J. Med 295:302-304.
limit the scope of significant variation in these genes.
ACKNOWLEDGEMENT
The authors thank Dr. C.R. Pillai, Malaria Research
Centre, Delhi for provoiding few isolates of P.vivax.
The research was supported by ICMR grant to Dr.
Umar Farooq.
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