Vol 27 No 2 December - The Indian Society for Parasitology

JPD : Vol. 27 (2), 2003
CSF production induced by L. donovani amastigote components
91
Table VIII: CFU-GM counts in spleen and BM of
a
LDAA-treated mice at different time intervals
b
b
LDAA CFU-GM/spleen CFU-GM/femur
3 3
(mg/kg) (x 10 ) (x 10 )
0 h 24 h 48 h 0 h 24 h 48 h
- 1.1±0.3 1.0±0.2 1.1±0.2 1.1±0.2 1.2±0.1 1.1±0.1
Control 1.0±0.1 1.0±0.2 1.1±0.3 1.1±0.2 1.1±0.3 1.0±0.1
antigen
0.01 1.1±0.1 1.3±0.2 1.1±0.2 1.0±0.2 1.4±0.3 1.1±0.2
0.10 1.1±0.2 2.3±0.6 1.8±0.4 1.2±0.2 1.8±0.3 1.7±0.2
1.00 1.0±0.2 2.7±0.8* 1.8±0.6 1.1±0.1 2.9±0.8* 1.5±0.2
5.00 1.1±0.3 2.7±0.7* 1.6±0.5 1.0±0.2 2.8±0.8* 1.5±0.2
10.00 1.0±0.3 2.4±0.6* 1.5±0.4 1.1±0.1 2.6±0.5* 1.6±0.3
HI-LDAA1.0±0.2 1.1±0.1 1.1±0.2 1.2±0.3 1.1±0.2 1.0±0.2
a 5 5
Spleen and BM scells (2x10 and 3x10 cells/dish, respectively)
from LDAA-, HI-LDAA- or control antigen (10 mg/kg)-treated
o
mice were cultured in CFU-GM medium (1 ml) for 48 h at 37 C in
5% CO2-air atmosphere.
b
After 7 days, CFU-GM colonies were counted. Data are the mean
number of colonies ± SD of three separate experiments, run in
triplicate.
*
Significantly (p