Cell death, transduction pathway and secretion tracked by flow ...

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III.Transduction pathway analysis • Differents methods For/Saponine, For/MeOH • Good, but not the best • For (2-4%)/Triton X-100 (0,1%) best for fresh blood samples : Best separation, good gating, scatters maintened Shankey et col, Cytometry, 2006;70B:259-69
III.Transduction pathway analysis • Monocytes Stimulated by LPS for different times • Stained with anti-CD14, fixed, permeabilized and stained for phospho ERK, SAP-JNK and p38 Images were made with Kaluza Tm Software, Beckman Coulter
Page 1 and 2: Cell death, transduction pathway an
Page 3 and 4: I.Critical point • Hydrodynamic f
Page 5 and 6: I.Optical Bench • Collects light
Page 7 and 8: I.Data representation « Dot Plot
Page 9 and 10: I.Data representation Images were m
Page 11 and 12: II.Basic death analysis • Membran
Page 13 and 14: II.Apoptosis analysis • Annexin V
Page 15 and 16: II.Apoptosis analysis • DNA fragm
Page 17 and 18: II.Apoptosis analysis • Mitochond
Page 19 and 20: II.Apoptosis analysis • Bax/Bcl-2
Page 21 and 22: II.Apoptosis analysis • Active fo
Page 23 and 24: III.Transduction pathway analysis
Page 27: III.Transduction pathway analysis
Page 33 and 34: IV.Cytokine secretion analysis •
Page 35 and 36: IV.Cytokine secretion analysis ELIS
Page 37: V.Conclusion • Flow cytometry is

Cell death, transduction pathway and secretion tracked by flow ...

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