Cell death, transduction pathway and secretion tracked by flow ...

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II.Apoptosis analysis • To resume : PS : Annexin V/7AAD or PI DNA fragmentation : Sub G1 peak/TUNEL Mitochondrial depolarisation : JC-1/DiOC(6)… Mitochondrial events : APO2.7/BcL-2/Bad… Caspase activity : FLICA/PhiPhilux Caspase active state : anti-cleaved caspase • Not only one, more you have more proofs you give…
III.Transduction pathway analysis • Apoptosis is a transduction pathway • Phospho (S70) BcL-2 more informative than BcL-2 for sensoring apoptotic death • A lot of transduction can be monitored by flow cytometry • Why? • Monitoring of cell pathway more easily, and more quickly than Western Blotting • Agonist/antagonist studies • Pharmacological studies
Page 1 and 2: Cell death, transduction pathway an
Page 3 and 4: I.Critical point • Hydrodynamic f
Page 5 and 6: I.Optical Bench • Collects light
Page 7 and 8: I.Data representation « Dot Plot
Page 9 and 10: I.Data representation Images were m
Page 11 and 12: II.Basic death analysis • Membran
Page 13 and 14: II.Apoptosis analysis • Annexin V
Page 15 and 16: II.Apoptosis analysis • DNA fragm
Page 17 and 18: II.Apoptosis analysis • Mitochond
Page 19 and 20: II.Apoptosis analysis • Bax/Bcl-2
Page 21: II.Apoptosis analysis • Active fo
Page 25 and 26: III.Transduction pathway analysis
Page 33 and 34: IV.Cytokine secretion analysis •
Page 35 and 36: IV.Cytokine secretion analysis ELIS
Page 37: V.Conclusion • Flow cytometry is

Cell death, transduction pathway and secretion tracked by flow ...

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