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Chapter 2<br />
Synthesis of substituted pyrimidines via Wittig<br />
Antibiotic Sensitivity Assay<br />
Sr.<br />
No.<br />
CODE<br />
No.<br />
Pseudomonas<br />
aeruginosa<br />
Proteus vulgaris<br />
Escherichia<br />
coli<br />
(Concentration250/500/ 1000 µG/ml)<br />
Staphylococcus<br />
aureus<br />
Candida<br />
albicans<br />
250 500 1000 250 500 1000 250 500 1000 250 500 1000 250 500 1000<br />
1. AW-01 R R 1.2 1.0 1.4 2.0 1.1 1.5 2.0 1.0 1.4 2.0 R 1.3 2.0<br />
2. AW-02 1.5 1.8 2.0 1.4 1.6 2.0 R R 1.8 R 1.2 1.8 1.0 1.4 2.0<br />
3. AW-03 1.1 1.4 2.0 1.0 1.3 2.0 R R R R R R R 1.3 2.0<br />
4. AW-04 R 1.3 1.8 R 1.1 1.4 R R 1.2 1.1 1.4 2.0 1.1 1.4 2.0<br />
5. AW-05 R 1.2 1.8 R 1.3 2.0 R 1.1 1.8 R 1.2 1.9 1.2 1.4 2.0<br />
6. AW-06 R R 1.2 R 1.2 1.8 R 1.1 1.8 1.0 1.3 2.0 R 1.2 2.0<br />
7. AW-07 1.1 1.4 2.0 R 1.2 1.8 1.0 1.3 2.0 1.1 1.4 2.0 R R 1.2<br />
8. AW-08 R 1.2 2.0 1.0 1.3 2.0 R 1.1 2.0 R 1.2 2.0 1.0 1.3 2.0<br />
9. AW-09 R R 1.2 R 1.2 2.0 1.0 1.3 2.0 R 1.0 2.0 R 1.4 2.0<br />
10. AW-10 1.1 1.3 1.8 1.5 1.8 2.0 R 1.0 1.7 R 1.2 2.0 1.0 1.3 2.0<br />
11. AW-11 R R 1.2 R R R 1.0 1.4 2.0 R 1.1 1.8 1.0 1.3 2.0<br />
12. AW-12 1.5 1.8 2.0 1.1 1.4 2.0 R 1.0 1.8 R 1.2 2.0 R 1.3 2.0<br />
13. AW-13 1.6 1.8 2.0 1.5 1.8 2.0 R 1.2 2.0 1.0 1.3 2.0 R 1.2 2.0<br />
14. AW-14 1.4 1.8 2.0 1.3 1.5 2.0 R R R R R R 1.0 1.3 2.0<br />
15. AW-15 R 1.2 1.5 R R R R R R R R R R 1.2 2.0<br />
16. AW-16 1.3 1.6 1.8 1.0 1.2 1.6 R 1.0 1.3 1.2 1.5 1.7 R 1.1 1.8<br />
17. AW-17 1.1 1.4 1.6 1.0 1.3 1.5 1.2 1.5 2.0 1.3 1.5 2.0 1.5 1.7 2.0<br />
18. AW-18 1.0 1.3 1.8 1.1 1.3 1.6 1.5 1.8 2.0 1.4 1.6 2.0 R 1.5 1.9<br />
19. AW-19 R R 1.2 R 1.3 1.8 1 1.2 2.0 1.1 1.6 2.0 1.3 1.6 2.0<br />
20. AW-20 R 1.3 1.8 1.1 1.3 1.2 1.0 1.2 1.4 1.0 1.2 1.5 1.2 1.5 1.7<br />
21. A 1.8 1.8 1.9 1.9 -<br />
22. CPD 2.2 2.1 2.1 2.2 -<br />
23. GF 1.8 1.9 2.0 2.0 -<br />
24. GRF - - - - 2.6<br />
25. FLC - - - - 2.8<br />
Note: Zone of inhibition interpretation is as follows<br />
1. Zone size < 1.0 cm- Resistent (R)<br />
2. Zone size 1.0 to 1.5 cm – Intermediate<br />
3. Zone size > 1.5 – Sensitive<br />
STD Antibiotic Sensitivity Assay Concentration 40 µg/ml<br />
A: Ampicillin GRF: Gresiofulvin<br />
CPD: Cefpodoxime FLC: Fluconazole<br />
GF: Gatifloxacin<br />
Department of Chemistry, <strong>Saurashtra</strong> university, Rajkot-360005 62