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CryoEM of ribosomes From 2D images to 3D map 3D reference map CryoEM images Virtual projections (Φ,θ) (Φ,θ,Ψ) x,y New 3D map N iterations Angular refinement
Translocation seen by CryoEM EF-G and ribosome interaction GTP GDP Valle, M., Zavialov, A., Sengupta, J., Rawat, U., Ehrenberg, M., and Frank, J. (2003). Cell 114, 123-134.
Page 1 and 2: Almost lost in translation CryoEM o
Page 3 and 4: Ribosomal structure •Two subunits
Page 5 and 6: Translocation by EF-G ? Pretransloc
Page 7: CryoEM of ribosomes EM of single pa
Page 11 and 12: At atomic level Flexible fitting Xr
Page 13 and 14: A new twist on translocation Single
Page 15 and 16: Dealing with heterogeneity Classifi
Page 17 and 18: Classification A new tool was devel
Page 19 and 20: Pretranslocation revisited by CryoE
Page 21 and 22: Pretranslocation revisited by CryoE
Page 23 and 24: The path of tRNAs through the ribos

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