lecture
lecture lecture
Pretranslocation revisited by CryoEM E.coli 70S ribosomes 70S · mRNA· fMet-tRNA · Leu-tRNA fMetLeu Leu-tRNA Variables Mg++ Association between ribosomal subunits Spermine Structure and stability of nucleic acids ML3D classification
Pretranslocation revisited by CryoEM Preliminary screening ML3D Full set Class 1 Class2 MgCl 2 0 mM Spermine 0 mM ___ ___ 18400 MgCl 2 20 mM Spermine 0 mM E P A E P A E P A P 18387 4576 7879 P/E MgCl 2 20 mM Spermine 2 mM E P A E P A E P A P A P/E A/P 15984 11958 4026
- Page 1 and 2: Almost lost in translation CryoEM o
- Page 3 and 4: Ribosomal structure •Two subunits
- Page 5 and 6: Translocation by EF-G ? Pretransloc
- Page 7 and 8: CryoEM of ribosomes EM of single pa
- Page 9 and 10: Translocation seen by CryoEM EF-G a
- Page 11 and 12: At atomic level Flexible fitting Xr
- Page 13 and 14: A new twist on translocation Single
- Page 15 and 16: Dealing with heterogeneity Classifi
- Page 17: Classification A new tool was devel
- Page 21 and 22: Pretranslocation revisited by CryoE
- Page 23 and 24: The path of tRNAs through the ribos
Pretranslocation revisited by CryoEM<br />
Preliminary screening<br />
ML3D<br />
Full set Class 1 Class2<br />
MgCl 2<br />
0 mM<br />
Spermine 0 mM<br />
___<br />
___<br />
18400<br />
MgCl 2<br />
20 mM<br />
Spermine 0 mM<br />
E P A E P A E P A<br />
P<br />
18387 4576<br />
7879<br />
P/E<br />
MgCl 2<br />
20 mM<br />
Spermine 2 mM<br />
E P A E P A E P A<br />
P A<br />
P/E A/P<br />
15984 11958 4026
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