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Pretranslocation revisited by CryoEM E.coli 70S ribosomes 70S · mRNA· fMet-tRNA · Leu-tRNA fMetLeu Leu-tRNA Variables Mg++ Association between ribosomal subunits Spermine Structure and stability of nucleic acids ML3D classification
Pretranslocation revisited by CryoEM Preliminary screening ML3D Full set Class 1 Class2 MgCl 2 0 mM Spermine 0 mM ___ ___ 18400 MgCl 2 20 mM Spermine 0 mM E P A E P A E P A P 18387 4576 7879 P/E MgCl 2 20 mM Spermine 2 mM E P A E P A E P A P A P/E A/P 15984 11958 4026
Page 1 and 2: Almost lost in translation CryoEM o
Page 3 and 4: Ribosomal structure •Two subunits
Page 5 and 6: Translocation by EF-G ? Pretransloc
Page 7 and 8: CryoEM of ribosomes EM of single pa
Page 9 and 10: Translocation seen by CryoEM EF-G a
Page 11 and 12: At atomic level Flexible fitting Xr
Page 13 and 14: A new twist on translocation Single
Page 15 and 16: Dealing with heterogeneity Classifi
Page 17: Classification A new tool was devel
Page 21 and 22: Pretranslocation revisited by CryoE
Page 23 and 24: The path of tRNAs through the ribos

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