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So …, translocation must be revisited<br />
What a “CryoEM-ist” would say<br />
Pseudo-native state<br />
No spatial restrictions (close-packing)<br />
Conformational changes<br />
Dynamic reactions<br />
L1<br />
E P A Dynamic<br />
interchange<br />
E<br />
P<br />
A<br />
L1<br />
E P A<br />
E P A<br />
Paradox: polymorphism vs resolution<br />
Structure-function relationship<br />
Conformational changes<br />
Heterogeneity<br />
Resolution<br />
Synchronization<br />
Classification<br />
Homogeneous groups
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