Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

EXTRACTION TECHNOLOGIES FOR MEDICINAL AND AROMATIC PLANTS
A
Figure 1: TLC chromatograms as visualized (A) under 254 nm UV and (B) after being
sprayed with anisaldehyde-sulphuric acid reagent
Besides developing the fi ngerprint profi le of the drug, the quantity
of DPH-1 was also estimated in different samples of the plant material.
For the purposes of analysis, 5 g moderately fi ne powder of drug material
was extracted with methanol in a Soxhlet apparatus for 4 h. The extract was
fi ltered and the volume was adjusted with methanol to 50 ml in a volumetric
fl ask. One milliliter of this solution was diluted to 10 ml in a volumetric fl ask
and used for analysis. A standard solution of DPH-1 was prepared by dissolving
4.95 mg DPH-1 in 10 ml methanol and diluting 0.5 ml of this solution to
10 ml in a volumetric fl ask. Six different concentrations of this solution were
applied in triplicate on a precoated TLC plate, which was developed using
mobile phase containing 90 volumes toluene and 10 volumes ethyl acetate.
The developed and dried plate was scanned at 305 nm in a TLC scanner and
the standard plot was constructed (Figure 2). One microliter of test solution
was similarly analyzed using the same conditions as used for DPH-1, and
the amount of DPH-1 in the test sample was calculated from the response
obtained in a TLC scanner. The analyzed drug samples showed large variations
in the content of DPH-1, ranging from below 0.3% to over 1.4%. The
method was validated according to ICH guidelines.
B
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