Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

13 COUNTER-CURRENT CHROMATOGRAPHY
Figure 14: Separation of WAP-8294A components using HSCCC
13.5.2.7 Other Examples of Separation of Phytoconstituents by
CCC
Apart from the previously mentioned examples, isolation and
purifi cation of polymethoxylated fl avones from tangerine peel, catechin constituents
from fi ve tea cultivars, rupestonic acid from the Chinese medicinal
plant Artemisia rupestris L., lycopene from tomato paste, spiramycin, gallic
acid from Cornus offi cinalis, lutein from the microalga Chlorella vulgaris,
naphthopyranone glycosides, salvianolic acid B from the Chinese medicinal
plant Salvia miltiorrhiza, dammarane saponins from Panax notoginseng, isofl
avan and pterocarpan glycosides from Astragalus membranaceus Bge. var.
mongholicus (Bge.), glycyrrhizin from the root of liquorice and active principles
from the roots of Sophora fl avescens have been carried out successfully
using HSCCC.
13.6 Advantages of CCC
• Quick (high throughput in preparative separation).
• Inexpensive (only solvent costs, which are 5 times less than
for other LC techniques).
• Gentle and versatile, for separation of varied compounds,
with less chance of decomposition.
• Able to resolve from milligrams to tens of grams on the
same instrument.
• Able to switch between normal and reverse phase at will.
• A CCC machine, which is a chromatographic column with a
liquid stationary phase, can be used as a liquid–liquid reactor
for chemical reactions involving a liquid catalyst.
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