Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

EXTRACTION TECHNOLOGIES FOR MEDICINAL AND AROMATIC PLANTS
Figure 13: Separation of Andrographolide and Neoandrographolide
from the leaves of Andrographis paniculata using HSCCC
13.5.2.6 Separation of WAP-8294A Components, a Novel Antimethicillin-resistant
Staphylococcus aureus Antibiotic,
using HSCCC
The WAP-8294A complex was isolated from the fermentation
broth of Lysobacter sp. WAP-8294 (Figure 14). The major component, WAP-
8294A2, shows strong activity against gram-positive bacteria including methicillin-resistant
Staphylococcus aureus (MRSA) and vancomycin-resistant
Enterococci in vitro, and also exhibited a potent activity against MRSA in
vivo.
The previous separations were unsatisfactory. Hence HSCCC
was applied. n-Butanol–ethyl acetate–aqueous 0.005 M trifl uoroacetic acid
(1.25:3.75:5, v/v/v) was used as biphasic solvent. A sample size of 25 mg
yielded pure fractions of three components (1–6 mg). The method will contribute
to the clinical development of WAP-8294A2 as an anti-MRSA agent.
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