Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

EXTRACTION TECHNOLOGIES FOR MEDICINAL AND AROMATIC PLANTS
Sample
Solvents
Solvent system
Ratio
Mobile
phase
n-Hexane–1-BuOH–H 2O 3:4:7 Lower
Phytolacca saponins CHCl 3–MeOH–2-PrOH–H 2O 5:6:1:4 Lower
Glycyrrhizin EtOAc–MeOH–H 2O 5:2:5 Lower
Carotenoids
Zeaxanthin n-Hexane–EtOAc–EtOH–H 2O 8:2:7:3 Lower
Lycopene n-Hexane–CH 3CN–CH 2Cl 2 20:13:7 Upper
Alkaloids
Aporphine alkaloids CH 2Cl 2–MeOH–5% HOAc 5:5:3 Lower
Naphthylisoquinoline
alkaloids
CHCl 3–EtOAc–MeOH–0.1 M HCl 5:3:5:3 Lower
Diterpene alkaloids n-Hexane–CH 2Cl 2–MeOH–H 2O 15:15:24:8 Lower
C 6H 6–CHCl 3–MeOH–H 2O 5:5:7:2 Lower
Tetramethylpyrazines
Chuanxiongzine n-Hexane–EtOAc–EtOH–H 2O 5:5:3:7 Lower
Glucosinolates PrOH–CH 3CN–(NH 4) 2SO 4–H 2O 10:5:12:10 Upper
Cyclodepsipeptides n-Heptane–EtOAc–MeOH–H 2O 2:8:2:8 Lower
13.5.1.2 Retention of the Stationary Phase
• Successful separation in HSCCC-CPC largely depends on
the amount of the stationary phase retained in the column.
In general, higher the retention of stationary phase, better
the peak resolution.
• The amount of stationary phase retained in the column is highly
correlated with the settling time of the two phases in a test tube.
• Measure the settling time of the two-phase solvent system
to be used for the separation.
• The procedure is as follows: the two phases are fi rst equilibrated
in a separatory funnel. Deliver 2 ml of each phase, a
total volume of 4 ml, into a test tube or graduated cylinder,
which is then capped. Gently invert the container for several
times and then immediately place it in an upright position to
measure the time required for the two phases to form clear
layers with a distinct interface.
• If the settling time is less than 20 seconds, the solvent system will
provide satisfactory retention of the stationary phase, usually over
50% of the total column capacity, in a proper range of flow rates.
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