Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

More documents

Recommendations

Info

11 PROCESS-SCALE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR MEDICINAL AND AROMATIC PLANTS The isolation and purifi cation of β-carotene from carrot involves: Column Shim-pack PREP-ODS (H) kit (250 x 20 mm i.d.) Mobile phase Ethanol (99.5%) Flow rate 10 ml /min Detection 480 nm monitor with photodiode array (PDA) Compound isolated β-carotene The isolation of anti-HIV compounds from Gleditsia japonica and Gymnocladus chinensis requires the following conditions: Column Mobile phase Detection Compound isolated C18-μBondpak (300 x 24.4 mm i.d.) Methanol-water (varying percentages) Refractive index detector Saponins Isolation of procyanidins from Vicia faba requires these conditions: Column Mobile phase Flow rate Detection Compounds isolated Sephadex LH-20 column (580 × 25 mm i.d.) Sequential elution with ethanol, ethanol:methanol, methanol and fi nally methanol:acetone Varying 280 nm (+)-gallocatechin-4-phloroglucinol; (−)-epigallocatechin- 4-phloroglucinol; (+)-gallocatechin; (−)-epicatechin-4- phloroglucinol; (+)-catechin-4-phloroglucinol; (+)-catechin and (−)-epicatechin Isolation of the anticancer compound taxol from Taxus yunnanensis is done with: Column D1 (4000 x 200 mm i.d.) packed with 956 polymeric resin Mobile phase Acetone:water (58:42) Flow rate Detection Compound isolated 79 ml/min 228 nm Taxol 192
EXTRACTION TECHNOLOGIES FOR MEDICINAL AND AROMATIC PLANTS 11.9 Conclusions Stepwise scale-up starting with analytical scale to process scale is an important issue that needs to be considered. Optimization of operating conditions is always useful for getting high purity phytomolecules. Thus, process-scale HPLC is the choice for isolating valuable molecules with desired purity for commercialization. Its signifi cance will continue to grow because of the increasing requirements for high-purity molecules. Bibliography Anonymous, 2007, Shimadzu application note SC-AP-LC-0186. Available at: http:// www2.shimadzu.com/apps/appnotes/app113.pdf Bouchet, N., Levesque, J. and Pousset, J. L., 2000, HPLC isolation, identifi cation and quantifi cation of tannins from Guiera senegalensis, Phytochemistry Annals, 11: 52–56 Brandt, A. and Kueppers, S., 2002, Practical aspects of preparative HPLC in pharmaceutical and development production, In: LC-GC-Europe, p. 2-5 Konoshima, T., Yasuda, I., Kashiwada, Y., Cosentino, L. M. and Lee, K. H., 1995, Anti-AIDS agents. XXI: Triterpenoid saponins as anti-HIV principles from fruits of Gleditsia japonica and Gymnocladus chinensis, and a structure-activity correlation, Journal of Natural Products, 58: 1372-1377 Lorenz, H., Sheehan, P. and Morgenstern, A. S., 2001, Coupling of simulated moving bed chromatography and fractional crystallisation for effi cient enantioseparation, Journal of Chromatography A, 908: 201-214 Merghem, R., Jay, M., Brun, N. and Voirin, B., 2004, Qualitative analysis and HPLC isolation and identifi cation of procyanidins from Vicia faba, Phytochemistry Annals, 15: 95-99 Pavli, V., Kmetec, V. and Tanja, T., 2004, Isolation of peptide components of bacitracin by preparative HPLC and solid phase extraction (SPE), Journal of Liquid Chromatography & Related Technology, 27: 2381-2396 Sargenti, S. R. and Vichnewski, W. 2000, Sonication and liquid chromatography as a rapid technique for extraction and fractionation of plant material, Phytochemistry Annals, 11: 69-73 Venn, R. F. (Ed.), 2000, Practicals and Practice of Bioanalysis, Taylor & Francis, London, p. 44-130 Yang, X., Liu, K. and Xie, M. 1998, Purifi cation of taxol by industrial preparative liquid chromatography, Journal of Chromatography A, 813: 201-204 193
Page 3 and 4:
Extraction Technologies for Medicin
Page 5:
Extraction Technologies for Medicin
Page 8 and 9:
CONTRIBUTORS Chapter 6 Aqueous Alco
Page 11 and 12:
EXTRACTION TECHNOLOGIES FOR MEDICIN
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117:
Page 120 and 121:
7 DISTILLATION TECHNOLOGY FOR ESSEN
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146: EXTRACTION TECHNOLOGIES FOR MEDICIN
Page 147: EXTRACTION TECHNOLOGIES FOR MEDICIN
Page 150 and 151: 9 SOLID PHASE MICRO-EXTRACTION AND
Page 195: EXTRACTION TECHNOLOGIES FOR MEDICIN
Page 200 and 201: 12 FLASH CHROMATOGRAPHY AND LOW PRE
Page 214 and 215: 13 COUNTER-CURRENT CHROMATOGRAPHY C
Page 216 and 217: 13 COUNTER-CURRENT CHROMATOGRAPHY K
Page 218 and 219: 13 COUNTER-CURRENT CHROMATOGRAPHY 1
Page 220 and 221: 13 COUNTER-CURRENT CHROMATOGRAPHY a
Page 224 and 225: 13 COUNTER-CURRENT CHROMATOGRAPHY W
Page 226 and 227: 13 COUNTER-CURRENT CHROMATOGRAPHY S
Page 230 and 231: 13 COUNTER-CURRENT CHROMATOGRAPHY
Page 232 and 233: 13 COUNTER-CURRENT CHROMATOGRAPHY E
Page 236 and 237: 13 COUNTER-CURRENT CHROMATOGRAPHY F
Page 240 and 241: 13 COUNTER-CURRENT CHROMATOGRAPHY H
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
show all

Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

Create successful ePaper yourself

Delete template?

Save as template?