Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

11 PROCESS-SCALE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR MEDICINAL AND AROMATIC PLANTS
The isolation and purifi cation of β-carotene from carrot involves:
Column
Shim-pack PREP-ODS (H) kit (250 x 20 mm i.d.)
Mobile phase Ethanol (99.5%)
Flow rate
10 ml /min
Detection
480 nm monitor with photodiode array (PDA)
Compound isolated β-carotene
The isolation of anti-HIV compounds from Gleditsia japonica
and Gymnocladus chinensis requires the following conditions:
Column
Mobile phase
Detection
Compound isolated
C18-μBondpak (300 x 24.4 mm i.d.)
Methanol-water (varying percentages)
Refractive index detector
Saponins
Isolation of procyanidins from Vicia faba requires these conditions:
Column
Mobile phase
Flow rate
Detection
Compounds isolated
Sephadex LH-20 column (580 × 25 mm i.d.)
Sequential elution with ethanol, ethanol:methanol, methanol
and fi nally methanol:acetone
Varying
280 nm
(+)-gallocatechin-4-phloroglucinol; (−)-epigallocatechin-
4-phloroglucinol; (+)-gallocatechin; (−)-epicatechin-4-
phloroglucinol; (+)-catechin-4-phloroglucinol; (+)-catechin and
(−)-epicatechin
Isolation of the anticancer compound taxol from Taxus yunnanensis
is done with:
Column
D1 (4000 x 200 mm i.d.) packed with 956 polymeric resin
Mobile phase Acetone:water (58:42)
Flow rate
Detection
Compound isolated
79 ml/min
228 nm
Taxol
192