Extraction Technologies for Medicinal and Aromatic ... - Capacity4Dev

9 SOLID PHASE MICRO-EXTRACTION AND HEADSPACE TRAPPING EXTRACTION
9.7.4.1.1 Preparation of Traps
Traps were prepared using silane-treated glass tubing (79 mm
x 6 mm) packed with 13 mg 60/80 mesh Tenax-TA (2,6-diphenyl-p-phenylene
oxide) polymers held in place by silanized glass wool. The traps were initially
conditioned at 330° C for 2 h under nitrogen gas at a 20 ml/min fl ow rate.
The traps were regenerated at 250° C for 1 h immediately before each
purge-and-trap experiment.
9.7.4.1.2 Thermal Desorption
Adsorbed volatile compounds were recovered from the trap directly
inside the GC injector. The desorption time and temperature were previously
determined. The injector temperature was 200° C and a loop of the
analytical column at the injector end was immersed in a liquid nitrogen-fi lled
Dewar fl ask to cryogenically trap the desorbed volatiles. Subsequently, the
injector glass liner (insert) was replaced with the trap to desorb volatiles.
Thermal desorption was carried out for 5 min with the split vent and septum
purge closed.
9.7.4.2 Headspace of Hedychium coronium
Hedychium coronium fl ower has a delicate, pleasant fragrance,
but the essential oil and concrete extracted by traditional methods usually
lose this fragrance. Thus, the headspace of the H. coronium fl ower was analyzed.
The essential oil of H. coronium fl owers, which was absorbed by XAD-4
resin, eluted by organic solvent and concentrated, had a fragrance similar to
the natural fragrance of H. coronarium fl owers.
9.7.4.3 Volatiles of White Hyacinths Isolated by Dynamic
Headspace Trapping
More than 70 constituents of white hyacinths can be identifi
ed by GC and GC-MS. The principal constituents are benzyl acetate and
(E,E)-α-farnesene. Beside these, sensorily important substances like indole,
oct-1-en-3-ol and phenylacetaldehyde were identifi ed. Minor traces of three
substituted pyrazines were detected by GC-sniffi ng. The advantages of the simultaneous
closed-loop stripping technique using various adsorbing agents
at the same time were demonstrated. By this method, artifact formation and
discrimination of individual components can be determined
9.7.4.4 Medical Materials Testing by Headspace Trapping-GC-MS
The new technology provided by the HS-40 Trap coupled with
a sensitive detection method such as GC-MS allows volatile organic compounds
in medical sutures to be analyzed easily at trace levels. Individual
compounds present in the sutures can be analyzed by GC-MS and identifi ed
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