Swine Disease Diagnostic Guide OL.indd - Newport Laboratories
Swine Disease Diagnostic Guide OL.indd - Newport Laboratories
Swine Disease Diagnostic Guide OL.indd - Newport Laboratories
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<strong>Disease</strong> Suspected Specimen Sample Preparation Laboratory Procedure<br />
Influenza<br />
Trachea, lung, nasal swabs<br />
(In viral transport media)<br />
Lung<br />
Refrigerate<br />
Virus isolation, PCR,<br />
subtyping, sequencing,<br />
BinaxNOW®<br />
10% Formalin Histopathology, (IHC)<br />
Mycoplasmosis<br />
PRRS<br />
Porcine circovirus<br />
(PCV2)<br />
Rotavirus<br />
Salmonellosis<br />
Serum Refrigerate Serology (HI/ELISA)<br />
Refrigerate<br />
Culture<br />
Lung<br />
10% Formalin Histopathology,<br />
PCR detection/speciation<br />
Serum Refrigerate Serology<br />
Lung<br />
Refrigerate/frozen Virus isolation, PCR<br />
10% Formalin Histopathology, IHC<br />
Refrigerate<br />
Serology, PCR, virus<br />
Serum<br />
isolation, sequencing<br />
Blood swab, semen Refrigerate PCR<br />
Lung, spleen, lymph nodes,<br />
kidney, intestine, pancreas<br />
Small intestine<br />
Small and large intestine, liver,<br />
lung, spleen, feces, mesenteric<br />
lymph node<br />
Refrigerate<br />
Virus isolation, PCR<br />
detection & sequencing<br />
10% Formalin Histopathology<br />
Refrigerate<br />
PCR (types A & C), VI,<br />
sequencing, RotaStat<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture-sensitivity,<br />
serotyping at reference lab<br />
Small and large intestine 10% Formalin Histopathology<br />
Salt Poisoning Brain 10% Formalin Histopathology<br />
Streptococcus suis<br />
Transmissible<br />
Gastroenteritis (TGE)<br />
Brain, lung, joint, liver, spleen<br />
Small intestine, ileum<br />
Refrigerate<br />
Culture-sensitivity,<br />
serotyping<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Histopathology<br />
10% Formalin IHC
<strong>Disease</strong> Suspected Specimen Sample Preparation Laboratory Procedure<br />
Abortion<br />
Actinobacillus<br />
pleuropneumoniae<br />
Arthritis<br />
Rhinitis<br />
Coccidiosis<br />
Colibacillosis<br />
Enteritis<br />
(non-specific)<br />
Clostridium<br />
perfringens<br />
Clostridium difficile<br />
Edema disease<br />
Erysipelas<br />
Haemophilus<br />
parasuis<br />
(Glasser's diseasepolyserositis)<br />
Ileitis<br />
(Lawsonia<br />
intracellularis)<br />
Fetus<br />
Refrigerate<br />
Culture-sensitivity<br />
Reference Lab - IgG, PCR,<br />
etc.<br />
Refrigerate<br />
Culture-sensitivity,<br />
Lung<br />
serotyping (via PCR)<br />
10% Formalin Histopathology<br />
Serum Refrigerate Serology<br />
Joint fluid, joint<br />
Snout, turbinates, nasal swabs<br />
Small intestine<br />
Small intestine<br />
Small intestine<br />
Small intestine<br />
Colon<br />
Fecal swabs<br />
Brain, small intestine<br />
Heart, lymph node, liver, spleen,<br />
joints, joint fluid<br />
Joint cavity or fluids, meninges,<br />
lung, exudate, heart<br />
Ileum, feces<br />
Refrigerate<br />
Culture-sensitivity<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture-sensitivity<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Smear<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture-sensitivity, PCR type<br />
& toxin test<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture-sensitivity, smear,<br />
anaerobic culture/typing,<br />
TGE Immunohistochemistry<br />
(IHC), rotavirus<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Anaerobic culture-sensitivity,<br />
toxin PCR (typing)<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture, A/B toxin ELISA,<br />
A/B toxin PCR<br />
Refrigerate<br />
Culture-sensitivity, PCR type<br />
& toxin test<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture-sensitivity<br />
10% Formalin Histopathology<br />
Refrigerate<br />
Culture-sensitivity, PCR<br />
10% Formalin Histopathology<br />
Refrigerate<br />
PCR at reference lab<br />
10% Formalin Histopathology
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