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ProSci Catalog
ProSci Catalog
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Antibody Product list
13100 Kirkham Way Tel: 858-513-2638<br />
Suite 207 1-888-513-9525<br />
Poway, CA 92064 Fax: 858-513-2692<br />
www.prosci-inc.com<br />
orders@prosci-inc.com<br />
Antibody Listed by Category<br />
Product Name Cat. No. Species Application Description Release Date<br />
Apoptosis-Chromatin condensation and DNA fragmentation factors<br />
Acinus (CP) 2215 H WB Inducer of chromatin condensation 4thQ 2000<br />
Acinus (CT) 2217 H WB Inducer of chromatin condensation 4thQ 2000<br />
Acinus (IN) 2219 H WB Inducer of chromatin condensation 4thQ 2000<br />
Acinus (NP) 2241 H WB Inducer of chromatin condensation 4thQ 2000<br />
AIF (CT) 2301 H M R WB Apoptosis Inducing Factor 4thQ 2000<br />
AIF (IN) 2267 H M R WB Apoptosis Inducing Factor<br />
AIF (NT) 2239 H WB Apoptosis Inducing Factor<br />
CAD / DFF40 (CT) 2011 M WB Caspase Activated DNase<br />
CAD / DFF40 (I17) 2007 M R WB Caspase Activated DNase<br />
CIDE-A (CT) 2085 H WB Cell death-Inducing DFF-like Effector<br />
CIDE-A (CT) 2089 M WB Cell death-Inducing DFF-like Effector 3rdQ 2000<br />
CIDE-B (CT) 2091 M WB Cell death-Inducing DFF-like Effector<br />
DFF40 / CAD (I18) 2107 H M R WB Caspase Activated DNase<br />
DFF40 / CAD (IN) 2155 H WB Caspase Activated DNase<br />
DFF40 / CAD (NT) 2153 H WB Caspase Activated DNase<br />
DFF45 / ICAD (CT) 1141 H WB DNA fragmentation factor & Inhibitor of CAD<br />
DFF45 / ICAD (NT) 1148 H WB, IHC DNA fragmentation factor & Inhibitor of CAD<br />
DNase II (CT) 2059 H WB DNase involved in apoptosis<br />
EndoG 3035 H M R WB Endonuclease G New<br />
ICAD / DFF45 (CT) 2003 M WB, IHC Inhibitor of CAD<br />
ICAD / DFF45 (NT) 2001 M WB, IHC Inhibitor of CAD<br />
Apoptosis-Caspases<br />
Casp-9 / Mch6 / Apaf-3 (IN1) 2071 H WB A new caspase<br />
Casp-9 / Mch6 / Apaf-3 (IN2) 2073 H WB A new caspase<br />
Casp-10 / FLICE2 (CT) 1128 H M R WB A new caspase<br />
Casp-12 (IN) 2327 H M R WB, IHC A new caspase 4thQ 2000<br />
Casp-12 (NT) 2325 H M R WB A casp. in Apoptosis & Alzheimer's Disease 4thQ 2000<br />
Casp-13 / ERICE 2245 H M R WB Evolutionarily Related ICE<br />
Apoptosis-CED4 homologue<br />
Apaf-1 (CT) 2015 H M R WB Apoptosis Protease Activating Factor<br />
Apaf-1 (NT) 2013 H M R WB Apoptosis Protease Activating Factor<br />
FLASH (CT) 2271 H WB Homolog of CED4 4thQ 2000<br />
Apoptosis-BcI-2 family<br />
Bcl10 / CIPER/ CLAP (NT) 2161 H WB, IHC Involved in apoptosis and tumor 4thQ 2000<br />
Bim / BOD (IN) 2065 H M R WB A new member in Bcl-2 family<br />
Bmf (CT) 3029 H WB A new member in Bcl-2 family New<br />
Bmf (NT) 3031 H M WB A new member in Bcl-2 family New<br />
Bnip3L / Nix (IN) 2289 H WB A new member in Bcl2 family 4thQ 2000<br />
PUMA (CT) bbc3 3041 H M WB A novel member in Bcl-2 family New<br />
PUMA (NT) bbc3 3043 H WB A novel member in Bcl-2 family New
Product Name Cat. No. Species Application Description Release Date<br />
Apoptosis-TRAIL and TRAIL receptors<br />
DcR1 / TRAIL-R3 (ED) 2179 H M R WB Decoy Receptor 1 for TRAIL<br />
DcR1 / TRAIL-R3 (ED2) 2299 H M R WB Decoy Receptor 1 for TRAIL 3rdQ 2000<br />
DcR2 / TRAIL-R4 (ID) 2021 H M R WB Decoy Receptor 2 for TRAIL<br />
DR4 / TRAIL-R1 (CT) 1139 H WB Death Receptor for TRAIL<br />
DR4 / TRAIL-R1 (NT) 1167 H WB Death Receptor for TRAIL<br />
DR5 / TRAIL-R2 2019 H M WB Death Receptor for TRAIL<br />
TRAIL / Apo-2L (CT) 1113 H WB Ligand for DR4 and DR5<br />
Apoptosis-Other cell death receptors<br />
DR3 / Wsl-1 / Apo-3 (CT) 1120 H M R WB Cell Death Receptor<br />
DR3 / Wsl-1 / Apo-3 (ED) 1158 H M WB Cell Death Receptor<br />
DR3 / Wsl-1 / Apo-3 (NT) 1166 H M WB Cell Death Receptor<br />
DR6 (NT) 2157 H WB Death Receptor 6<br />
Apoptosis-TNF and Fas related molecules<br />
ADAM10 / KUZ (CT) 2051 H WB TNFα converting enzyme<br />
ARTS 3025 H WB An apoptosis related protein New<br />
DcR3 / TR6 (NT) 2140 H M R WB Decoy Receptor for FASL and LIGHT<br />
SODD (NT) 2143 H M R WB Silencer Of Death Domains<br />
TACE/adam17 (CT) 1131 H M R WB TNFα converting enzyme<br />
Apoptosis-Regulating kinases<br />
ASK1 / MAPKKK5 (CT) 1151 H WB Apoptosis Signal-regulation Kinase<br />
DAPK2 2323 H M R WB Death-Associated Protein kinase 2 1stQ 2001<br />
DRAK1 (NT) 2147 H WB DAPK Related Apoptosis-inducing Kinase<br />
DRAK2 (CT) 2149 H WB DAPK Related Apoptosis-inducing Kinase<br />
RICK / RIP2 (CT) 2183 H WB RIP-like Interacting CLARP Kinase<br />
RICK / RIP2 (NT) 2075 H M R WB RIP-like Interacting CLARP Kinase<br />
RIP3 2283 M R WB Mouse Receptor Interacting Protein 3 1stQ 2001<br />
ZIPK (IN) 2067 H M R WB Apoptosis inducing kinase<br />
Apoptosis-Inhibitory molecules<br />
ARC (CT) 2081 H WB Apoptosis Repressor with CARD domain<br />
ARC (NT) 2185 H M R WB Apoptosis Repressor with CARD domain<br />
mFLIP / I-FLICE (CT) 1156 M WB FLICE inhibitory protein<br />
FLIP / I-FLICE (CT) 1161 H M R WB FLICE inhibitory protein<br />
FLIP / I-FLICE (NT) 1159 H M R WB FLICE inhibitory protein<br />
FLIPs (CT) 2055 H WB FLICE inhibitory protein 3rdQ 2000<br />
ILP-2 3017 H M R WB A novel member in IAP family New<br />
Livin, KIAP 2505 H WB A novel member in IAP family New<br />
Smac / DIABLO (CT) 2409 H M R WB IAPs binding protein 1stQ 2001<br />
mSmac / DIABLO (CT) 2411 H M R WB, IHC IAPs binding protein 1stQ 2001<br />
Survivin / TIAP (CT) 2235 H WB, IHC A new inhibitor of apoptosis 3rdQ 2000<br />
Survivin / TIAP (NT) 2233 H WB, IHC A new inhibitor of apoptosis 3rdQ 2000<br />
mSurvivin / TIAP (CT) 2237 M WB, IHC A new inhibitor of apoptosis 4thQ 2000
Product Name Cat. No. Species Application Description Release Date<br />
Apoptosis-Adapter molecules<br />
ASC 2287 H WB A novel CARD-containing adaptor protein 1stQ 2001<br />
CARD9 2515 H WB A new CARD-containing protein New<br />
DEDAF 2227 H M R WB A novel regulator of apoptosis 3rdQ 2001<br />
Daxx (CT) 1163 H M WB Adapter Molecule<br />
F1Aα (CT) 2279 H M R WB A novel death receptor-binding protein 1stQ 2001<br />
RAIDD / CRADD (CT) 1117 H WB Adapter Molecule<br />
RAIDD / CRADD (IN) 1115 H WB Adapter Molecule<br />
Signal Transduction<br />
CIKS/Act1 (CT) 2499 H WB A novel NF-κB activator 3rdQ 2001<br />
CIKS/Act1 (NT) 2501 H WB A novel NF-κB activator 3rdQ 2001<br />
DOK (CT) 1107 H WB Tyrosine kinase substrate p62DOK<br />
IKKα / IKK-1 (C1) 2025 H WB, IHC IκB kinase alpha<br />
IKKα / IKK-1 (C2) 2115 H WB IκB kinase alpha<br />
IKKα / IKK-1 (C3) 2117 H WB IκB kinase alpha<br />
IKKβ / IKK-2 (C3) 2121 H WB IκB kinase beta<br />
IKKε / IKKi (CT) 2329 H WB IκB Kinase epsilon 1stQ 2001<br />
IKKγ / NEMO / FIP3 (NT) 2335 H M R WB IκB Kinase gamma 1stQ 2001<br />
IL-1RAcP (C1) 2131 H WB IL-1R accessory protein<br />
IL-1RAcP (C2) 2129 H WB IL-1R accessory protein<br />
IRAK (CT) 1007 H M R WB, IP IL-1R associated kinase<br />
IRAK-2 (C2) 2123 H WB IL-1R associated kinase<br />
IRAK-2 (CT) 2213 H WB IL-1R Associated Kinase<br />
IRAK-M 2355 H M R WB A novel IL-1R Associated Kinase 1stQ 2001<br />
MADD / DENN (CT) 1150 H M R WB Activator of MAPK pathway<br />
MYD88 (CT) 2127 H M R WB Adapter in IL-1 & LPS signal<br />
MYD88 (IN) 2125 H WB Adapter in IL-1 & LPS signal<br />
NAK/TBK1 (CT) 2351 H M WB NF-κB activating kinase 2ndQ 2001<br />
NIK (CT) 1129 H WB NF-κB inducing kinase<br />
SIRPα / SHPS-1 (CT) 1125 H M R WB Tyrosine kinase substrate<br />
STAT1 (CT) 1021 H M R WB, IP JAK activated transcription<br />
HIV and chemokine receptors<br />
Bonzo / STRL33 (CT) 1170 H WB HIV coreceptor<br />
Bonzo / STRL33 (NT2) 2209 H WB HIV coreceptor<br />
CCR5 (NT) 1112 H WB HIV & chemokine receptor<br />
CCR8 (EL) 2097 H WB HIV & chemokine receptor<br />
CX3CR1 / V28 (EL) 2201 H WB HIV & chemokine receptor<br />
CX3CR1 / V28 (NT) 2093 H M R WB, FACS HIV & chemokine receptor<br />
CXCR4 (EL) 1012 H M WB HIV & chemokine receptor<br />
CXCR4 (NT) 1009 H M WB, IP, IHC HIV & chemokine receptor<br />
DC-SIGN (CT) 2347 H WB A novel HIV biding protein 3rdQ 2001<br />
DC-SIGN (ED) 2349 H WB A novel HIV biding protein 3rdQ 2001<br />
Eotaxin (CT) 1123 H WB CCR3 ligand<br />
GPR15 / BOB (NT) 2101 H M R WB HIV coreceptor
Product Name Cat. No. Species Application Description Release Date<br />
Neurobiology<br />
APP (AβNT) 2133 H M R WB Amyloid protein precursor<br />
APP (CT) 2136 H M R WB Amyloid protein precursor<br />
BACE / Asp2 (CT) 2253 H M WB Beta-site APP Cleaving Enzyme<br />
BACE2 / Asp1 (NT) 2247 H M R WB Beta-site APP Cleaving Enzyme 2 4thQ 2000<br />
BACE2 / Asp1 (CT) 2249 H WB Beta-site APP Cleaving Enzyme 2 4thQ 2000<br />
GFRα-1 / GDNFR α (IN) 1133 H M R WB GDNF receptor<br />
GFRα-2 / GDNFR β (IN) 1135 H M R WB Neurturin receptor<br />
GFRα-3 / GDNFR γ (IN) 1137 H M R WB Persephin receptor<br />
Neurturin 1121 H WB Ligand for GFRα-2<br />
P53 Network<br />
Chk2 (NT) 2391 H M R WB A novel check point kinase 2ndQ 2001<br />
MTBP (IN2) 2447 H WB MDM2 binding protein 2ndQ 2001<br />
p53DINP1, SIP 3045 H M R WB Apoptosis-associated p53 target New<br />
p53R2 (NT) 2383 H M R WB A novel ribonucleotide reductase 2ndQ 2001<br />
PERP 2451 H WB A mediator of p53 induced apoptosis 2ndQ 2001<br />
PID/MTA2 2443 H M R WB Involved in P53 deacetylation 2ndQ 2001<br />
Immunology<br />
APRIL (ED) 2223 H WB A new member in the TNF family 2ndQ 2001<br />
APRIL (ED2) 2415 H M WB A new member in the TNF family 2ndQ 2001<br />
BAFF / BLyS / Thank / Tall-1 (CT) 2221 H M R WB B cell Activating Factor in TNF Family<br />
IL-21 (IN) 2465 H WB Interleukin 21, related to IL2 and IL15 3rdQ 2001<br />
IL21R (ED) 2469 H M R WB Interleukin 21 receptor 3rdQ 2001<br />
IL21R (NT) 2471 H M R WB Interleukin 21 receptor 3rdQ 2001<br />
IL22R (NT) 2497 H R WB Interleukin 22 receptor 3rdQ 2001<br />
IL22R (CT) 2489 H R WB Interleukin 22 receptor 3rdQ 2001<br />
TCCR (NT) 2481 H WB A new T cell cytokine receptor 3rdQ 2001<br />
TCCR (CT) 2483 H WB A new T cell cytokine receptor 3rdQ 2001<br />
WB-Western blot / IHC-immunohistochemistry / IP-immunoprecipitation / NT-N-terminus<br />
CT-C-terminus / ED-extracellular domain / ID-intracellular domain / IN-intermediate domain<br />
NP-N-terminus of the peptide / CP-C-terminus of the peptide<br />
EL-extracelluar loop / FACS-Flow Cytometry / H-human / M-mouse / R-rat
peptide Product list
13100 Kirkham Way Toll free: 888-513-9525<br />
Suite 207 Tel: 858-513-2638<br />
Poway, CA 92064 Fax: 858-513-2692<br />
www.prosci-inc.com<br />
orders@prosci-inc.com<br />
Peptide List<br />
Product Name Cat. No. Source Sequences<br />
Acinus (CP) peptide 2215P Human TRTALHGVKWPQSNPK<br />
Acinus (CT) peptide 2217P Human SRSTPVRDRGGRR<br />
Acinus (IN) peptide 2219P Human DTSENRPENDVPEPP<br />
Acinus (NP) peptide 2241P Human DDPVRTAQVPSPPRGK<br />
ADAM10 (CT) peptide 2051P Human PQRQRPRESYQMGHMRR<br />
AIF (CT) peptide 2301P Human KDGEQHEDLNEVAK<br />
AIF (IN) peptide 2267P Human PKSATEQSGTGIRSE<br />
AIF (NT) peptide 2239P Human KQKKAALSASEGEE<br />
Apaf-1 (CT) peptide 2015P Human TFYTNGTNLKKIHVSPDFKT<br />
Apaf-1 (NT) peptide 2013P Human HREALEKDIKTSYIMDHC<br />
APP (AbNT) peptide 2133P Human CDAEFRHDSGY<br />
APP (CT) peptide 2136P Human CGYENPTYKFFEQMQN<br />
APRIL (ED) peptide 2223P Human GTGGPSQNGEGYP<br />
APRIL (ED2) peptide 2415P Human CIRSMPSHPDRAYNS<br />
ARC (CT) peptide 2081P Human PDPEPEPDFEERDESEDS<br />
ARC (NT) peptide 2185P Human GNAQERPSETIDRERKR<br />
ASC peptide 2287P Human RESQSYLVEDLERS<br />
BACE (CT) peptide 2253P Human CLRQQHDDFADDISLLK<br />
BACE2 (CT) peptide 2249P Human CQRRPRDPEVVNDESS<br />
BACE2 (NT) peptide 2247P Human APTPGPGTPAERHADG<br />
BAFF (CT) peptide 2221P Human EEGDELQLAIPRENAQ<br />
BCL-10 (NT) peptide 2161P Human APSLTEEDLTEVKKDC<br />
Bim (IN) peptide 2065P Human AERPPQLRPGAPTSLQTEP<br />
Bnip3L (IN) peptide 2289P Human DAQHESGQSSSRGSSH<br />
Bonzo (CT) peptide 1170P Human KSSEDNSKTFSASHNVEATS<br />
Bonzo (NT2) peptide 2209P Human GFSSFNDSSQEEHQD<br />
CAD (CT) peptide 2011P Mouse KLECDRSRIYRPQTGS<br />
CAD (I17) peptide 2007P Mouse YNGSYFDRGAEASSRL<br />
Casp-10 (CT) peptide 1128P Human ISAQTPRPPMRRWSSVS<br />
Casp-12 (NT) peptide 2325P Murine AARRTHERDPIYKIKG<br />
Casp-13 peptide 2245P Human ETVAGPDESVGSAAT<br />
Casp-9 (IN1) peptide 2071P Human EDIQRAGSGSRRDQAR<br />
Casp-9 (IN2) peptide 2073P Human ASTSPEDESPGSNPEPDATP<br />
Caspase-12 (IN) peptide 2327P Murine CTPSSPSESKRKVEDDE<br />
CCR5 (NT) peptide 1112P Human SSPIYDINYYTSEPC<br />
CCR8 (EL) peptide 2097P Human CYSFYNQQTLKWKIFTNFK<br />
Chk2 (NT) peptide 2391P Human SRESDVEAQQSHGSSAC<br />
CIDE-A (CT) peptide 2085P Human DDKEERPSLRSQAKGRFT<br />
CIKS/Act1 (CT) peptide 2499P Human REEEYVAPPRGPLPT<br />
CIKS/Act1 (NT) peptide 2501P Human PPQLQETRMNRSIP<br />
CX3CR1 (EL) peptide 2201P Human CLGDYPEVLQEIWPV<br />
CX3CR1 (NT) peptide 2093P Human DQFPESVTENFEYDDLAEAC<br />
CXCR4 (EL) peptide 1012P Human DRYICDRFYPNDLWV<br />
CXCR4 (NT) peptide 1009P Human MEGISIYTSDNYTE<br />
DAP kinase 2 peptide 2323P Human ARRKALHPRRRSSTS<br />
Daxx (CT) peptide 1163P Human TSVATQCDPEEIIVLSDSD<br />
DC-SIGN (CT) peptide 2347P Human CSRDEEQFLSPAPATPNPPPA<br />
DC-SIGN (ED) peptide 2349P Human CYFMSNSQRNWHDSITA<br />
DcR1 (ED) peptide 2179P Human CVEEFGANATVETPAAEET<br />
DcR1 (ED2) peptide 2299P Human CKEGTFRNENSPE
Product Name Cat. No. Source Sequences<br />
DcR2 (ID) peptide 2021P Human GGPERVHRVLFRRRS<br />
DcR3 (NT) peptide 2140P Human AETPTYPWRDAETGERC<br />
DEDAF peptide 2227P Human TPKGDMSAVNDESF<br />
DFF40 (IN) peptide 2155P Human NGSYFDRGAKGGSRLC<br />
DFF40 (NT) peptide 2153P Human QKPKSVKLRALRSPRKC<br />
DFF40/CAD (I18) peptide 2107P Mouse EGLESRFRNKSGYLRYS<br />
DFF45 (CT) peptide 1141P Human KASPPGDLQNPKRARQDPT<br />
DFF45/35 (NT) peptide 1148P Human EVTGDAGVPESGEIRTLKPC<br />
DNase II (CT) peptide 2059P Human CNGMARKPSRAYKI<br />
DR3 (CT) peptide 1120P Human ERMGLDGCVEDLRSRLQRGP<br />
DR3 (ED) peptide 1158P Human APCTEPCGNSTCLVCPQDT<br />
DR3 (NT) peptide 1166P Human QGGTRSPRCDCAGDFH<br />
DR4 (CT) peptide 1139P Human CDSGKFIYLEDGTGSAVSLE<br />
DR4 (NT) peptide 1167P Human ASGTEAAAATPSKVWGSSAG<br />
DR5 (CT) peptide 2019P Human KIEDHLLSSGKFMYLEGNAD<br />
DR6 (NT) peptide 2157P Human QPEQKASNLIGTYRHC<br />
DRAK1 (NT) peptide 2147P Human EKPGSGGSSPGATSGC<br />
DRAK2 (CT) peptide 2149P Human CSKRFRFDDSLPNPHE<br />
Eotaxin peptide 1123P Human DSMKYLDQKSPTPKP<br />
F1Aα peptide 2279P Human DINYQDQIPRTLEE<br />
FLASH (CT) peptide 2271P Human SERFQQLMKLFEKSKC<br />
FLIP (CT) peptide 1161P Human NGYMYDWNSRVSAKEKYY<br />
FLIP (NT) peptide 1159P Human SAEVIHQVEEALDTDEKE<br />
FLIPs (CT) peptide 2055P Human QKSLKDPSNNFRMITPYAH<br />
GFRα-1 (IN) peptide 1133P Human KNKPLGPAGSENEI<br />
GFRα-3 (IN) peptide 1137P Human RFHRQLFSQDWADS<br />
GPR15 (NT) peptide 2101P Human YYATSPNSDIRETHSH<br />
ICAD (CT) peptide 2003P Mouse RRSPLPGEPQRPKRAKRDSS<br />
ICAD (NT) peptide 2001P Mouse ELSRGASAPDPDDVRPLKPC<br />
IKKα (C1) peptide 2025P Human CLGHLSTIIHEANEEQGNS<br />
IKKα (C2) peptide 2115P Human TPQDGETSAQMIEENLN<br />
IKKα (C3) peptide 2117P Human CTQSSARSLVGSSLEGA<br />
IKKβ (C3) peptide 2121P Human CSKVRGPVSGSPDSMNASR<br />
IKKε/IKK-i (CT) peptide 2329P Human NRIIERLNRVPAPPDV<br />
IKKγ/NEMO (NT) 2335P Human CQYQAPDMDTLQIHVME<br />
IL-1RAcP (C2) peptide 2129P Human KWKGEKSKYPQGRFWK<br />
IL-1RAcP (CT) peptide 2131P Human KKSPRRSSSDEQGLSYSSLKN<br />
IL-21 (IN) peptide 2465P Human TCPSCDSYEKKPPKE<br />
IL-21R (ED) peptide 2469P Human NITDQSGNYSQECGS<br />
IL-21R (NT) peptide 2471P Human CPDLVCYTDYLQT<br />
IL-22R (CT) peptide 2489P Human DSLFRGLALTVQWES<br />
IL-22R (NT) peptide 2497P Human QSSNFENILTWDSGPE<br />
IRAK (CT) peptide 1007P Human DRQGPEESDEFQS<br />
IRAK-M peptide 2355P Human CSSKFSWDEYEQYKKE<br />
IRAK2 (C2) peptide 2123P Human APWAGAATPLLPTENGEGR<br />
IRAK2 (CT) peptide 2213P Human READSSSEACVGLEPPQDVT<br />
MADD (CT) peptide 1150P Human AAVHSSEEDLRTPPRPVSS<br />
mCIDE-A (CT) peptide 2089P Mouse GDTEEQPSPKPSTKG<br />
mCIDE-B (CT) peptide 2091P Mouse EGADRWQWHGQRHLHS<br />
mFLIP (CT) peptide 1156P Mouse DKVYAWNSGVSSKEKYS<br />
mSmac/DIABLO (CT) peptide 2411P Murine SDEGADQEEEAYLRED<br />
MyD88 (CT) peptide 2127P Human CTKSWFWTRLAKALSLP<br />
MyD88 (IN) peptide 2125P Human CDFQTKFALSLSPGAHD<br />
MTBP (IN2) peptide 2447P Human GAVECFEEEDSNSRESLS<br />
NAK/TBK1 (CT) peptide 2351P Human ERFGSLTMDGGLRNVD
Product Name Cat. No. Source Sequences<br />
NIK (CT) peptide 1129P Human SFAWSWRVKHGQLENRP<br />
NTN (NT) peptide 1121P Human DAHSRYHTVHELSARE<br />
p53R2 (NT) peptide 2383P Human GDPERPEAAGLDQDER<br />
PID/MTA2 peptide 2443P Human PAPSHPASTNEPIVLED<br />
RAIDD (CT) peptide 1117P Human CHNGLRAVEVDPSLLLHMLE<br />
RAIDD (IN) peptide 1115P Human CAGDRLTGIPSHILNSSPSD<br />
RICK (CT) peptide 2183P Human KLKDNKQMGLQPYPE<br />
RICK (NT) peptide 2075P Human PTIPYHKLADLRYLSRGASG<br />
RIP3 peptide 2283P Murine AQFGRGRGWQPFHK<br />
SIRPα (CT) peptide 1125P Human KPEPSFSEYASVQVPRK<br />
Smac/DIABLO (CT) peptide 2409P Human EERAESEQEAYLRED<br />
SODD (NT) peptide 2143P Human SALRRSGYGPSDGPSC<br />
Survivin (CT) peptide 2235P Human CKKVRRAIEQLAAMD<br />
Survivin (NT) peptide 2233P Human MGAPTLPPAWQP<br />
Survivin peptide 2237P Mouse AKTTRQSIEQLAA<br />
TACE (CT) peptide 1131P Human ASFKLQRQNRVDSKETE<br />
TRAIL (CT) peptide 1113P Human CTNEHLIDMDHEASFFGA<br />
ZIPK (IN) peptide 2067P Human RRRNVRGEDSGRKPERRRLK
lysate Product list
13100 Kirkham Way Toll free: 888-513-9525<br />
Suite 207 Tel: 858-513-2638<br />
Poway, CA 92064 Fax: 858-513-2692<br />
www.prosci-inc.com<br />
orders@prosci-inc.com<br />
Product Name <strong>Catalog</strong> No. Application Size<br />
Cell Lysate<br />
293 whole cell lysate 1210 Western blot 200 µg<br />
3T3 whole cell lysate 1212 Western blot 200 µg<br />
U937 whole cell lysate 1215 Western blot 200 µg<br />
A431 whole cell lysate 1202 Western blot 200 µg<br />
A549 whole cell lysate 1203 Western blot 200 µg<br />
HeLa whole cell lysate 1201 Western blot 200 µg<br />
HepG2 whole cell lysate 1211 Western blot 200 µg<br />
HL60 whole cell lysate 1209 Western blot 200 µg<br />
Jurkat whole cell lysate 1205 Western blot 200 µg<br />
K562 whole cell lysate 1204 Western blot 200 µg<br />
MDA-MB-361 whole cell lysate 1217 Western blot 200 µg<br />
MOLT4 whole cell lysate 1206 Western blot 200 µg<br />
PC-3 whole cell lysate 1216 Western blot 200 µg<br />
Raji whole cell lysate 1207 Western blot 200 µg<br />
SW1353 whole cell lysate 1214 Western blot 200 µg<br />
T24 whole cell lysate 1213 Western blot 200 µg<br />
THP-1 whole cell lysate 1208 Western blot 200 µg<br />
Human Tissue Lysate<br />
Human brain tissue lysate 1303 Western blot 200 µg<br />
Human heart tissue lysate 1301 Western blot 200 µg<br />
Human kidney tissue lysate 1305 Western blot 200 µg<br />
Human liver tissue lysate 1304 Western blot 200 µg<br />
Human lung tissue lysate 1302 Western blot 200 µg<br />
Human pancreas tissue lysate 1307 Western blot 200 µg<br />
Human small intestine tissue lysate 1308 Western blot 200 µg<br />
Human spleen tissue lysate 1306 Western blot 200 µg<br />
Human Tumor Tissue Lysate<br />
Lysate List<br />
Human brain tumor lysate 1322 Western blot 200 µg<br />
Human breast tumor lysate 1323 Western blot 200 µg<br />
Human colon normal tissue lysate 1341-N Western blot 200 µg<br />
Human colon tumor tissue lysate 1341-T Western blot 200 µg<br />
Human kidney tumor lysate 1324 Western blot 200 µg<br />
Human liver normal tissue lysate 1342-N Western blot 200 µg<br />
Human liver tumor tissue lysate 1342-T Western blot 200 µg<br />
Human lung tumor lysate 1321 Western blot 200 µg
Product Name <strong>Catalog</strong> No. Application Size<br />
Mouse Tissue Lysate<br />
Mouse brain tissue lysate 1403 Western blot 200 µg<br />
Mouse heart tissue lysate 1401 Western blot 200 µg<br />
Mouse kidney tissue lysate 1405 Western blot 200 µg<br />
Mouse liver tissue lysate 1404 Western blot 200 µg<br />
Mouse lung tissue lysate 1402 Western blot 200 µg<br />
Mouse skeletal muscle tissue lysate 1407 Western blot 200 µg<br />
Mouse small intestine tissue lysate 1408 Western blot 200 µg<br />
Mouse spleen tissue lysate 1406 Western blot 200 µg<br />
Rat Tissue Lysate<br />
Rat brain tissue lysate 1463 Western blot 200 µg<br />
Rat kidney tissue lysate 1465 Western blot 200 µg<br />
Rat liver tissue lysate 1464 Western blot 200 µg<br />
Rat lung tissue lysate 1462 Western blot 200 µg<br />
Rat skeletal muscle tissue lysate 1467 Western blot 200 µg<br />
Rat spleen tissue lysate 1466 Western blot 200 µg
Antibody Data Sheets
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Anti-IRAK (CT)<br />
CATALOG No.: 1007<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli including IL-1, TNFα and<br />
LPS. A serine/threonine protein kinase associated with IL-1<br />
receptor (IRAK) and its homologue mouse pelle-like protein<br />
kinase (mPLK) were identified recently (1,2). IRAK is<br />
associated with the IL-1 receptor subunits IL-1RI and IL-<br />
1RAcP after IL-1 binding and serves as a signaling molecule<br />
to mediate IL-1 response (3). IRAK mediates a signaling<br />
cascade leading to NF-κB activation by members in IL-1<br />
family including IL-1 and a novel cytokine IL-18 (also termed<br />
IGIF) (1,4).<br />
SOURCE:<br />
Rabbit anti-IRAK polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 700 to 712 of human<br />
IRAK (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:1000 dilution and for immunoprecipitation with 2 to 4 µg<br />
per sample. THP-1 or HeLa whole cell lysate can be used as<br />
positive control and an 80 kDa band should be detected in<br />
the lysate from non-activated cells. It is human, mouse, and<br />
rat reactive, and has no cross response to IRAK2. For<br />
research use only.<br />
STORAGE: It is supplied as purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
Western blot analysis of IRAK<br />
in THP-1 (THP) and HeLa<br />
(HL) whole cell lysates with<br />
anti-IRAK at 1:2000 dilution.<br />
REFERENCES:<br />
1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the<br />
interleukin-1 receptor. Science 1996;271:1128-31.<br />
2. Trofimova M; Sprenkle AB; Green M; Sturgill TW; Goebl MG;<br />
Harrington MA. Developmental and tissue-specific expression of<br />
mouse pelle-like protein kinase. J Bio Chem 1996; 271: 17609-<br />
12.<br />
3. Jianing Huang, Xiong Gao, Shyun Li, and Zhaodan Cao.<br />
Recruitment of IRAK to the interleukin 1 receptor complex requires<br />
interleukin-1 receptor accessory protein. Proc Natl Acad Sci USA<br />
1997;94:12829-12832<br />
4. Robinson D, Shibuya K, Mui A, Zonin F, Murphy E, Sana T,<br />
Hartley SB, Menon S, Kastelein R, Bazan F, O'Garra A. IGIF does<br />
not drive Th1 development but synergizes with IL-12 for interferongamma<br />
production and activates IRAK and NF-κB. Immunity<br />
1997;7:571-581<br />
(RD1299)
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Anti-CXCR4 (NT) Fusin, LESTR, HUMSTR<br />
CATALOG NO.: 1009<br />
BACKGROUND:<br />
Human immunodeficiency virus (HIV) and related viruses<br />
require coreceptors, in addition to CD4, to infect target cells.<br />
Some G protein-coupled receptors including CCR5, CXCR4,<br />
CCR3, CCR2b and CCR8 in the chemokine receptor family,<br />
and four new human molecules GPR15, STRL33, GPR1 and<br />
V28 were recently identified as HIV coreceptors (1). Among<br />
them, CXCR4 (fusin, LESTR or HUMSTR) is a principal<br />
coreceptor for T-cell tropic strains of HIV-1 fusion and entry<br />
of human white blood cells (2,3). CXCR4 is also required for<br />
the infection by dual-tropic strains of HIV-1 and mediates<br />
CD-4 independent infection by HIV-2 (4,5). The α-chemokine<br />
SDF-1 is the ligand for CXCR4 and prevents infection by T-<br />
tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-<br />
gp120 complex before HIV enters target cells (8). CXCR4<br />
messenger RNA levels correlated with HIV-1 permissiveness<br />
in diverse human cell types (2). Antibodies to CXCR4 block<br />
HIV-1 and HIV-2 fusion and infection of human target cells<br />
(2,5,10). The amino-terminal domain and the second<br />
extracellular loop of CXCR4 serve as HIV binding sites<br />
(10,11).<br />
SOURCE:<br />
Rabbit anti-CXCR-4 polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 1 to 14 of human<br />
CXCR4 (3,9).<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:1000 to 1:2000 dilution and for immunoprecipitation and<br />
immunocytochemistry. HeLa whole cell lysate can be used<br />
as positive control. It is human and mouse reactive. For<br />
research use only.<br />
Western blot analysis of<br />
CXCR4 in HeLa total cell<br />
lysate with anti-CXCR4 (NT)<br />
at 1:2000 dilution.<br />
REFERENCES:<br />
1. Dimitrov DS. Cell 1997;91:721-730<br />
2. Feng Y et al. Science 1996;272:872-7.<br />
3. Berson JF et al. J Virol 1996;70:6288-95.<br />
4. Doranz BJ et al. Cell 1996;85:1149-1158<br />
5. Endres MJ et al. Cell 1996;87:745-756<br />
6. Bleul CC et al. Nature 1996;382:829-833<br />
7. Oberlin E et al. Nature 1996;382:833-835<br />
8. Lapham CK et al. Science 1996;274:602-5.<br />
9. Leoetscher M et al. J Biol Chem 1994;269:232-237<br />
10. Brelot A et al. J Virol 1997;71:4744--954751.<br />
11. Lu Z et al. Proc Natl Acad Sci USA 1997;94:6426-6431<br />
(RD1299)<br />
STORAGE:<br />
It is supplied as purified IgG fraction, 100 µg in 200 µl of<br />
PBS containing 0.02% sodium azide. Store at 4°C, stable for<br />
one year.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-CXCR4 (EL) Fusin, LESTR, HUMSTR<br />
CATALOG NO.: 1012<br />
BACKGROUND:<br />
Human immunodeficiency virus (HIV) and related viruses<br />
require coreceptors, in addition to CD4, to infect target cells.<br />
Some G protein-coupled receptors including CCR5, CXCR4,<br />
CCR3, CCR2b and CCR8 in the chemokine receptor family,<br />
and four new human molecules GPR15, STRL33, GPR1 and<br />
V28 were recently identified as HIV coreceptors (1). Among<br />
them, CXCR4 (fusin, LESTR or HUMSTR) is a principal<br />
coreceptor for T-cell tropic strains of HIV-1 fusion and entry<br />
of human white blood cells (2,3). CXCR4 is also required for<br />
the infection by dual-tropic strains of HIV-1 and mediates<br />
CD-4 independent infection by HIV-2 (4,5). The α-chemokine<br />
SDF-1 is the ligand for CXCR4 and prevents infection by T-<br />
tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-<br />
gp120 complex before HIV enters target cells (8). CXCR4<br />
messenger RNA levels correlated with HIV-1 permissiveness<br />
in diverse human cell types (2). Antibodies to CXCR4 block<br />
HIV-1 and HIV-2 fusion and infection of human target cells<br />
(2,5,10). The amino-terminal domain and the second<br />
extracellular loop of CXCR4 serve as HIV biding sites<br />
(10,11).<br />
APPLICATION: This polyclonal antibody can be used for<br />
Western blot at 1:500 to 1:1000 dilution. HeLa whole cell<br />
lysate can be used as positive control. It is human and<br />
mouse reactive. For research use only.<br />
SOURCE:<br />
Rabbit anti-CXCR-4 polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 182 to 196<br />
in the second extracellular loop (EL) of human CXCR4<br />
(3,9).<br />
Western blot analysis of<br />
CXCR4 in HeLa total cell<br />
lysate with anti-CXCR4<br />
(EL) at 1:1000 dilution.<br />
STORAGE:<br />
It is supplied as purified IgG fraction, 100 µg in 200 µl of<br />
PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
REFERENCES:<br />
1. Dimitrov DS. Cell 1997;91:721-730<br />
2. Feng Y et al. Science 1996;272:872-7.<br />
3. Berson JF et al. J Virol 1996;70:6288-95.<br />
4. Doranz BJ et al. Cell 1996;85:1149-1158<br />
5. Endres MJ et al. Cell 1996;87:745-756<br />
6. Bleul CC et al. Nature 1996;382:829-833<br />
7. Oberlin E et al. Nature 1996;382:833-835<br />
8. Lapham CK et al. Science 1996;274:602-5.<br />
9. Leoetscher M et al. J Biol Chem 1994;269:232-237<br />
10. Brelot A et al. J Virol 1997;71:4744--954751<br />
11. Lu Z et al. Proc Natl Acad Sci USA 1997;94:6426-6431<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-STAT1α<br />
CATALOG No.: 1021<br />
BACKGROUND:<br />
STATs (signal transducers and activators of transcription)<br />
are a family of cytoplasmic latent transcription factors that<br />
are activated to regulate gene expression in response to a<br />
large number of extracellular signaling polypeptides<br />
including cytokines, interferons, and growth factors. After<br />
phosphorylation by JAK tyrosine kinases, STATs enter the<br />
nucleus to regulate transcription of many different genes.<br />
Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a,<br />
Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a<br />
wide activation profile (1,2). STAT1 is activated by many<br />
different ligands including IFN family (IFN-α, IFN-β, IFN-γ<br />
and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-<br />
CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-<br />
1) (3). STAT1 has two forms, the 91 kDa STAT1α and the<br />
84 kDa STAT1β which are encoded by the same gene with<br />
splicing variant (4).<br />
SOURCE:<br />
Rabbit anti-STAT1α polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 712 to 750 of human<br />
STAT1α (4). The sequences differ from the murine<br />
corresponding sequences by four amino acids.<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:1000 to 1:2000 and for immunoprecipitation at 2 to 4 µg<br />
per sample. It is human, mouse, and rat reactive. Whole cell<br />
lysate from HeLa, Jurkat, or A431 cells can be used as<br />
positive control and a 91 kDa band can be detected. No<br />
reaction to the 84 kDa STAT1β. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at -20°C. Stable for<br />
one year at 2-8°C.<br />
Western blot analysis<br />
of STAT1α in whole<br />
cell lysates from HeLa<br />
(H), Jurkat (J), A431<br />
(A), K562 (K), and<br />
NIH3T3 (N) cells,<br />
respectively, with anti-<br />
STAT1α at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Leonard WJ, O'Shea JJ. Jaks and STATs: biological<br />
implications. Annu Rev Immunol 1998;16:293-322.<br />
2. Schindler C, Darnell JE Jr. Transcriptional responses to<br />
polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem<br />
1995;64:621-51.<br />
3. Darnell JE Jr. STATs and gene regulation. Science<br />
1997;277:1630-5.<br />
4. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr.<br />
Proteins of transcription factor IS GF-3: one gene encodes the 91-<br />
and 84-kDa ISGF-3 proteins that are activated by interferon alpha.<br />
Proc Natl Acad Sci USA 1992;89:7836<br />
(RD1299)
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Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-p62 dok<br />
CATALOG No.: 1107<br />
STORAGE:<br />
It is supplied as affinity purified IgG with immunogenic<br />
BACKGROUND:<br />
Signals from most growth factors and cytokines are<br />
peptide, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at -20°C. Stable for one year at 2-8°C.<br />
transduced by receptor tyrosine kinases or non-receptor<br />
tyrosine kinases. Activated tyrosine kinases phosphorylate<br />
their substrates, which mediate the cellular response to<br />
extracellular stimuli. A long-sought major substrate termed<br />
p62 dok (downstream of tyrosine kinase) for many tyrosine<br />
kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and<br />
activated EGF, PDGF, IGF, VEGF and insulin receptors was<br />
Western blot analysis of<br />
p62 dok in Jurkat (Jur) and<br />
THP-1 (THP) total cell<br />
lysates with anti-p62 dok at<br />
1:1000 dilution.<br />
identified recently from human and mouse by several<br />
laboratories (1,2). Upon phosphorylation, p62 dok<br />
forms a<br />
complex with the ras GTPase-activating protein (RasGAP)<br />
(1-3). p62 dok represents a new family with very recently<br />
identified p56 dok (4)<br />
SOURCE:<br />
Rabbit anti-p62 dok polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 425 to 439 of human<br />
p62 dok (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of p62 dok<br />
expression by Western blot at 1:1000 to 1:2000 dilution.<br />
Whole cell lysate from Jurkat cells can be used as positive<br />
control and a 62 kDa band should be detected. This antibody<br />
is for research use only.<br />
REFERENCES:<br />
1. Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R,<br />
Stillman B, Clarkson B p62(dok): a constitutively tyrosinephosphorylated,<br />
GAP-associated protein in chronic myelogenous<br />
leukemia progenitor cells. Cell 1997;88:197-204.<br />
2. Yamanashi Y, Baltimore D Identification of the Abl- and rasGAPassociated<br />
62 KDa protein as a docking protein, Dok. Cell<br />
1997;88:205-211.<br />
3. Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley<br />
LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane<br />
tyrosine residues couple the Eph family receptor EphB2/Nuk to<br />
specific SH2 domain proteins in neuronal cells. EMBO J<br />
1997;16:3877-3888.<br />
4. Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi<br />
R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD.<br />
Molecular cloning and characterization of p56(dok-2) defines a new<br />
family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-<br />
4830.<br />
(RD1299)
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Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-CCR5 (NT)<br />
CATALOG NO.: 1112<br />
BACKGROUND:<br />
Human immunodeficiency virus (HIV) and related virus<br />
require coreceptors, in addition to CD4, to infect target cells.<br />
Some G protein-coupled receptors including CCR5, CXCR4,<br />
CCR3, CCR2b and CCR8 in the chemokine receptor family,<br />
and four new human molecules GPR15, STRL33, GPR1 and<br />
V28 were recently identified as HIV coreceptors (1,2).<br />
Among them, CCR5 (CC-CKR-5) is a principal coreceptor for<br />
macrophage- and dual-tropic HIV-1 strains fusion and entry<br />
of human white blood cells (3,4). CCR5 is required for the<br />
infection by HIV-1, HIV-2, and SIV (1,2). The β-chemokines<br />
RANTES, MIP-1α and MIP-1β are the ligands for CCR5 and<br />
prevent infection by M-tropic HIV-1 (3-5). CXC5 associates<br />
with the surface CD4-gp120 of HIV complex and leads to<br />
membrane fusion and virus entry of target cells (6,7). The<br />
amino-terminal domain and the extracellular loops of CCR5<br />
serve as HIV biding sites (8,9). CCR5 messenger RNA is<br />
expressed in lymphoid organs and monocytes.<br />
SOURCE:<br />
Rabbit anti-CCR5 polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 6 to 20 of human<br />
CCR5 (10,11).<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:1000 to 1:2000 dilution. THP-1 whole cell lysate can be<br />
used as positive control and an approximate 40 kDa band<br />
can be detected. For research use only.<br />
Western blot analysis of<br />
CCR5 in THP-1 whole cell<br />
lysate with anti-CCR5 (NT)<br />
at 1:1000 dilution.<br />
REFERENCES:<br />
1. Dimitrov, DS. Cell 1997;91:721-30<br />
2. Littman, DR. Cell 1998;93:677-80<br />
3. Deng, H, et al. Nature 1996;381:661-6<br />
4. Dragic, T, et al. Nature 1996;381:667-73<br />
5. Coochi, F. et al. Science 1995;270:1811-5.<br />
6. Wu, L. et al. Nature 1996;384:179-83<br />
7. Trkola , A. et al. Nature 1996;384:184-7<br />
8. Doranz, BJ. et al. J Virol 1997;71:6305-14<br />
9. Dragic, T, et al. J Virol 1998;72:279-85<br />
10. Raport, CJ, et al. J Biol Chem 1996;271:17161-6<br />
11. Combadiere, C. et al. J Leuk Biol 1996;60:147-52<br />
(RD0900)<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-TRAIL (CT) Apo-2L<br />
CATALOG No.: 1113<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand in the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. A novel<br />
member in the TNF family was recently identified and<br />
designated TRAIL (for TNF-related apoptosis-inducing<br />
ligand) and Apo-2L (for Apo-2 ligand) 1,2 . TRAIL is a type II<br />
membrane protein and expressed in a variety of human<br />
tissues. Two novel death domain containing receptors DR4<br />
and DR5 have been identified as the receptor for TRAIL 3-6 .<br />
Like TNF and Fas ligand, TRAIL induces apoptosis and NFκB<br />
activation in many tissues and cells.<br />
SOURCE:<br />
Rabbit anti-TRAIL (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 261 to 277<br />
of human TRAIL (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of TRAIL<br />
by Western blot at 1:500 dilution. For research use only.<br />
STORAGE:<br />
It is supplied as ion chromatography purified IgG, 100 µg in<br />
200 µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year. Azide free antibody is available.<br />
Western blot analysis of<br />
TRAIL in HeLa cell lysate<br />
containing 10, 2.5, or 1 ng of<br />
recombinant protein<br />
containing extracellular<br />
domain of TRAIL with anti-<br />
TRAIL (CT) at 1 µg/ml.<br />
REFERENCES:<br />
1. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl<br />
JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al.<br />
Identification and characterization of a new member of the TNF<br />
family that induces apoptosis. Immunity 1995;3:673-682<br />
2. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A;<br />
Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new<br />
member of the tumor necrosis factor cytokine family. J. Biol. Chem.<br />
1996;271:12687-90<br />
3. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R;<br />
Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand<br />
TRAIL. Science; 1997;276:111-113<br />
4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K,<br />
Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2<br />
(DR5) signal FADD-dependent apoptosis and activate NF-κB.<br />
Immunity 1997;7:831-836<br />
5. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist<br />
decoy receptor and a death domain-containing receptor for TRAIL.<br />
Science 1997;277:815-818<br />
6. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M,<br />
Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI,<br />
Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced<br />
apoptosis by a family of signaling and decoy receptors. Science<br />
1997;277:818-821<br />
(RD0500)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-RAIDD (IN) CRADD<br />
CATALOG No.: 1115<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand of the TNF family through their<br />
death domain (DD)-containing receptors, TNFR1 and Fas.<br />
The death signals are transduced by a group of DDcontaining<br />
adapter molecules. A novel cell death adapter<br />
was recently identified by two independent groups and<br />
designated RAIDD (RIP-associated ICH-1/CED-3-<br />
homologous protein with DD) and CRADD (caspase and RIP<br />
adapter with DD) 1,2 . RAIDD contains a DD and a CARD (for<br />
caspase recruitment domain) which interact with RIP and<br />
caspase, respectively, to transduce death signals 1,3 . RAIDD<br />
is constitutively expressed in many tissues and mediates<br />
apoptosis caused by Fas and TNFR-1.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
Western blot analysis of<br />
RAIDD in whole cell<br />
lysates from HeLa (H) or<br />
K562 (K) cells with anti-<br />
RAIDD (IN) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule.<br />
Nature 1997;385:86-89<br />
SOURCE:<br />
Rabbit anti-RAIDD (IN) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 99 to 117 of<br />
human RAIDD 1 .<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of RAIDD<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from HeLa or K562 cells can be used as positive control and<br />
a 22 kDa band should be detected. This antibody is for<br />
research use only.<br />
2. Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G,<br />
Fernandes-Alnemri T, Alnemri ES. CRADD, a novel human<br />
apoptotic adaptor molecule for caspase-2, and FasL/tumor<br />
necrosis factor receptor-interacting protein RIP. Cancer Res 1997<br />
57:615-619<br />
3. Hofmann K, Bucher P, Tschopp J. The CARD domain: a new<br />
apoptotic signalling motif. Trends Biochem Sci 1997;22:155-15<br />
(RD1299)
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Anti-RAIDD (CT) CRADD<br />
CATALOG No.: 1117<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand of the TNF family through their<br />
death domain (DD)-containing receptors, TNFR1 and Fas.<br />
The death signals are transduced by a group of DDcontaining<br />
adapter molecules. A novel cell death adapter<br />
was recently identified by two independent groups and<br />
designated RAIDD (RIP-associated ICH-1/CED-3-<br />
homologous protein with DD) and CRADD (caspase and RIP<br />
adapter with DD) 1,2 . RAIDD contains a DD and a CARD (for<br />
caspase recruitment domain) which interact with RIP and<br />
caspase, respectively, to transduce death signals 1,3 . RAIDD<br />
is constitutively expressed in many tissues and mediates<br />
apoptosis caused by Fas and TNFR-1.<br />
SOURCE:<br />
Rabbit anti-RAIDD (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 181 to 199<br />
of human RAIDD 1 .<br />
STORAGE:<br />
It is supplied as purified IgG fraction, 100 µg in 200 µl of<br />
PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
Western blot analysis of<br />
RAIDD in HeLa total cell lysate<br />
with anti-RAIDD (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor<br />
molecule. Nature 1997;385:86-89<br />
2. Ahmad M, Srinivasula SM, Wang L, Talanian RV,<br />
Litwack G, Fernandes-Alnemri T, Alnemri ES. CRADD, a<br />
novel human apoptotic adaptor molecule for caspase-2,<br />
and FasL/tumor necrosis factor receptor-interacting protein<br />
RIP. Cancer Res 1997 57:615-619<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of RAIDD<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from HeLa cells can be used as positive control and a major<br />
22 kDa band should be detected. This antibody is for<br />
research use only.<br />
3. Hofmann K, Bucher P, Tschopp J. The CARD domain: a<br />
new apoptotic signalling motif. Trends Biochem Sci<br />
1997;22:155-15<br />
(RD1299)
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Anti-DR3 (CT) Wsl-1, Apo-3, TRAMP, LARD<br />
CATALOG No.: 1120<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand of the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. A novel<br />
cell death receptor was recently identified by several groups<br />
independently and designated DR3, Wsl-1, Apo-3, TRAMP<br />
and LARD 1-5 . The ligand for this novel cell death receptor<br />
has not yet been defined. DR3 is highly expressed in the<br />
tissues enriched in lymphocytes including PBL, thymus and<br />
spleen. Like TNFR1, DR3 induces apoptosis and NF-κB<br />
activation.<br />
SOURCE:<br />
Rabbit anti-DR3 (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 398 to 417 of human<br />
DR3 1,2 .<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:500 to 1:1000 dilution. Jurkat total cell lysate can be used<br />
as positive control and a 59 kDa band should be detected. It<br />
is human, mouse, and rat reactive and has no cross reaction<br />
to other death receptors. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG fraction, 100 µg in 200 µl of<br />
PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
Western blot analysis of<br />
DR3 in Jurkat total cell<br />
lysate with anti-DR3 (CT)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan<br />
DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996;274:990-2.<br />
2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT;<br />
Grinham CJ; Brown R; Farrow SN. Nature, 1996;384:372-5.<br />
3. Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL;<br />
Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996;6:1669-76.<br />
4. Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V;<br />
Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al.<br />
Immunity, 1997;6:79-88.<br />
5. Screaton GR; Xu XN; Olsen AL; Cowper AE; Tan R; McMichael<br />
AJ; Bell JI. Proc. Nat. Acad. Sci. USA. 1997;94:4615-9.<br />
(RD1299)
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Anti- Neurturin<br />
CATALOG No.: 1121<br />
BACKGROUND:<br />
Glial cell line-derived neurotrophic factor (GDNF) plays key<br />
roles in the control of vertebrate neuron survival and<br />
differentiation. A novel neurotrophic factor was recently<br />
cloned from human and mouse and designated neurturin (1,<br />
2). Physiological responses to neurturin (NTN) require the<br />
presence of receptor tyrosine kinase RET and a novel<br />
glycosylphosphatidylinositol linked receptor NTNRα (3,4).<br />
The cDNAs encoding NTNRα from human, rat, chicken, and<br />
mouse have been cloned recently (3-8) and termed<br />
GDNFRβ, Ret ligand 2 (RETL2) or TGF-β-related<br />
neurotrophic factor receptor 2 (TrnR2) and nominated as<br />
GFRα-2 recently (9). NTN binds to and forms a complex with<br />
GFRα-2 and the Ret PTK and activates the RET receptor<br />
tyrosine kinase pathway. Both NTN and GDNF can activate<br />
the MAP kinase and phosphatidylinositol 3-kinase pathways<br />
and play a critical role in the development of many neuronal<br />
populations. Neurturin and GDNF define a new family of<br />
neurotrophic factors.<br />
SOURCE:<br />
Rabbit anti-neurturin polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 178 to 193 of human<br />
neurturin (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
neurturin by Western blot. An approximate 14 kDa band of<br />
the full length recombinant NTN was detected. For research<br />
use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at -20°C.<br />
Stable for one year at 2-8°C.<br />
Western blot analysis of NTN in<br />
HeLa cell lyaste containing 10 or<br />
2.5 ng of full length recombinant<br />
NTN with anti-neurturin at 1:500.<br />
REFERENCES:<br />
1. Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative<br />
glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470<br />
2.<br />
2. Heuckeroth RO, Kotzbauer P, Copeland NG, et al. Neurturin, a novel<br />
neurotrophic factor, is localized to mouse chromosome 17 and human<br />
chromosome 19p13.3. Genomics 1997;44(1):137-40<br />
3. Klein RD, Sherman D, Ho WH, et al. GPI-linked protein that interacts with<br />
Ret to form a candidate neurturin receptor. Nature 1997;387:717-721<br />
4. Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a<br />
GPI-linked receptor and the Ret receptor tyrosine kinase. Nature<br />
1997;387:721-724<br />
5. Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that<br />
mediates neurturin and GDNF signaling through Ret. Neuron 1997;18:793-<br />
802<br />
6. Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic<br />
factor-dependent RET activation can be mediated by two different cellsurface<br />
accessory proteins. Proc Natl Acad Sci USA 1997;94:6238-6243<br />
7. Suvanto P, Wartiovaara K, Lindahl M, et al. Cloning, mRNA distribution<br />
and chromosomal localisation of the gene for glial cell line-derived<br />
neurotrophic factor receptor beta, a homologue to GDNFR-alpha. Hum Mol<br />
Genet 1997;6:1267-1273<br />
8. Wang CY, Ni J, Jiang H, et al. Cloning and characterization of glial cell<br />
line-derived neurotrophic factor receptor-B: a novel receptor for members of<br />
glial cell line-derived neurotrophic factor family of neurotrophic factors.<br />
Neuroscience 1998;83(1):7-14<br />
9. Nomenclature Committee. Nomenclature of GPI-linked receptors for the<br />
GDNF ligand family. GFRα Neuron 1997;19:485 (RD0500)
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Anti-Eotaxin<br />
CATALOG No.: 1123<br />
BACKGROUND:<br />
Chemokines play a key role in inflammation. The CC<br />
chemokine eotaxin is a potent and specific eosinophil<br />
chemoattractant that is expressed by a variety of cell types<br />
in certain inflammatory conditions (1,2). Some G-protein<br />
coupled chemokine receptors are also utilized as virus<br />
coreceptors for fusion and infection of cells. The eotaxin<br />
receptor CCR3 is required for HIV-1 entry into target cells<br />
such as microglia and eotaxin inhibits the infection of HIV-1<br />
(3,4).<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 1123P).<br />
Western blot analysis of<br />
eotaxin in HeLa cell lysate<br />
containing 10, 2.5, or 1 ng of<br />
full length recombinant<br />
eotaxin with anti-eotaxin at 1<br />
µg/ml.<br />
SOURCE:<br />
This polyclonal antibody was raised in rabbits against a<br />
peptide corresponding to amino acids 83 to 97 of human<br />
eotaxin (1,2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of eotaxin<br />
by Western blot at 0.5 to 1 µg/ml. The recombinant protein<br />
can be used as positive control and monomer and<br />
homodimer are detected at approximate 9 and 18 kDa. For<br />
research use only.<br />
REFERENCES:<br />
1. Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human<br />
eotaxin, an eosinophil-selective CC chemokine, and identification<br />
of a specific eosinophil eotaxin receptor, CC chemokine receptor 3.<br />
J Biol Chem 1996;271:7725-30<br />
2. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human<br />
eosinophil chemoattractant, eotaxin. Expression, receptor binding,<br />
and functional properties suggest a mechanism for the selective<br />
recruitment of eosinophils. J Clin Invest 1996;97:604-12<br />
3. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are coreceptors<br />
for HIV-1 infection of microglia. Nature.<br />
1997;385(6617):645-9<br />
4. Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors<br />
CCR3 and CCR5 facilitate infection by primary HIV -1 isolates. Cell.<br />
1996;85:1135-48<br />
(WD0500)<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.
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Anti-SIRPα (CT) SHPS-1, MyD-1, BIT, p84<br />
CATALOG No.: 1125<br />
BACKGROUND:<br />
Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2<br />
are critical regulators in the intracellular signaling pathways<br />
that result in cell responses such as mitosis, differentiation,<br />
migration, survival, transformation or death. SHP-2 is a<br />
signal transducer for several receptor tyrosine kinases and<br />
cytokine receptors. A novel SHP-2 associated glycoprotein<br />
was recently cloned from human, rat, mouse and cattle by<br />
several labs and was designated SIRPα (1), SHPS-1 (2,3),<br />
MyD-1 (4), BIT (5,6) and p84 (7). SIRPα is a new gene<br />
family containing at least fifteen members. SIRPα is a<br />
substrate of many activated tyrosine kinases such as insulin<br />
receptor, EGFR, PDGFR and src, and a specific docking<br />
protein for SHP-2 (1,2,5,8). SIRPα has regulatory effects on<br />
cellular responses induced by serum, growth factors, insulin,<br />
oncogenes, growth hormones and cell adhesion and plays a<br />
general role in different physiological and pathological<br />
processes (1,2,5,8).<br />
SOURCE:<br />
Rabbit anti-SIRPα polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 487 to 503 of human<br />
SIRPα1 (1). The sequences of immunogenic peptide differ<br />
from those of mouse, rat and bovine by one amino acid (1-<br />
7).<br />
cells can be used as positive control and 75-110 kDa bands<br />
should be detected. It is human, mouse, and rat reactive<br />
and recognizes SIRPα1, 2 and 3. For research use only.<br />
Western blot analysis of<br />
SIRPα in THP-1 whole<br />
cell lysate with anti-<br />
SIRPα at 1:2000<br />
dilution.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
REFERENCES:<br />
1. Kharitonenkov A, et al. Nature 1997;386:181-186.<br />
2. Fujioka Y, et al. Mol Cell Biol 1996;16:6887-6899<br />
3. Yamao T, et al. Biochem Biophys Res Commun 1997;231:61-67<br />
4. Brooke GP, et al. Eur J Immunol 1998;28:1-11<br />
5. Ohnishi H, et al. J Biol Chem 1996;271:25569-25574<br />
6. Ohnishi H, et al. Genomics 1997;40:504-506<br />
7. Comu S, et al. J Neurosci 1997;17:8702-8710<br />
8. Stofega MR, et al. J Biol Chem 1998;273:7112-7117<br />
(RD1299)<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:1000 to 1:2000 dilution. Whole cell lysate from THP-1
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Anti-Caspase-10 (CT) FLICE2<br />
CATALOG No.: 1128<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain (DD)- containing<br />
adapter molecules and members of the ICE/CED-3 protease<br />
family. A novel ICE/CED-3 protease was identified recently,<br />
designated FLICE2 and Mch4 1,2 and renamed as caspase-<br />
10. Caspase-10 has two death effector domains (DEDs) that<br />
bind to the DED in the adapter molecule FADD and recruits<br />
both TNFR1 and CD95 to form complexes with these<br />
receptors. Caspase-10 is therefore involved in the CD95 and<br />
TNFR1 induced apoptosis 1 . Caspase-10 cleaves and<br />
activates caspase-3, -4, -6, -7, -8 and -9, which causes the<br />
proteolytic cleavage of many key proteins such as PARP.<br />
Cleavage of PARP occurs in many different systems during<br />
apoptosis and is the hallmark of programmed cell death 3 .<br />
Caspase-10 is expressed in many tissues and cell lines 1,2 .<br />
SOURCE:<br />
Rabbit anti-caspase-10 polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 505 to 521<br />
of human FLICE2 1 .<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
caspase-10 by Western blot at 1:500 to 1:1000 dilution.<br />
HeLa whole cell lysate can be used as positive control and a<br />
59 kDa band can be detected. This antibody only recognizes<br />
FLICE2 form of caspase-10. It is human, mouse and rat<br />
reactive. For research use only.<br />
Western blot analysis of<br />
caspase-10 in HeLa (H), Jurkar<br />
(J), A431 (A), K562 (K), and<br />
NIH3T3 (3T3) whole cell<br />
lysates with anti-caspase-10 at<br />
1:1000 dilution.<br />
STORAGE:<br />
It is supplied as purified IgG fraction, 100 µg in 200 µl of<br />
PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
REFERENCES:<br />
1. Vincenz.C. and Dixit,V.M. Fas-associated death domain protein<br />
interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3<br />
homologue, is proximally involved in CD95- and p55-mediated<br />
death signaling. J. Biol. Chem. 1997;272:6578-6583<br />
2. Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM,<br />
Wang L Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G,<br />
Alnemri ES. In vitro activation of CPP32 and Mch3 by Mch4, a<br />
novel human apoptotic cysteine protease containing two FADD-like<br />
domains. Proc. Natl. Acad. Sci. USA. 1996;93:7464-7469<br />
3. Cohen GM. Caspases: the executioners of apoptosis. Biochem J<br />
1997;326:1-16<br />
(RD1299)
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Anti-NIK (CT)<br />
CATALOG No.: 1129<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli including IL-1, TNFα, LPS<br />
and mitogens. A serine/threonine protein kinase which<br />
mediates NF-κB activation by IL-1, TNFα and CD95 was<br />
identified recently and designated NIK (for NF-κB inducing<br />
kinase) (1). NIK is an activator of IκB kinase alpha and beta<br />
(IKKα and IKKβ) (2-5). Therefore, NIK is a key molecule in<br />
the NF-κB signaling pathway leading to the induction of a<br />
variety of gene expression in response to proinflammatory<br />
cytokines and bacteria products.<br />
SOURCE:<br />
Rabbit anti-NIK polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 931 to 947 of human<br />
NIK (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of NIK by<br />
Western blot. 293 cell lysate can be used as positive control.<br />
STORAGE:<br />
It is supplied as 100 µg purified IgG in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 1129P).<br />
293 cell lysate, 200 µg/100 µl, is available for positive<br />
control (<strong>Catalog</strong> No. 1210).<br />
REFERENCES:<br />
1. Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3Krelated<br />
kinase involved in NF-kappaB induction by TNF, CD95 and<br />
IL-1. Nature 1997;385:540-544<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M.<br />
Identification and characterization of an IkappaB kinase. Cell<br />
1997;90:373-383<br />
3. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DV. IkappaB<br />
kinase-beta: NF-kappaB activation and complex formation with<br />
IkappaB kinase-alpha and NIK. Science 1997;278:866-869<br />
4. Ling L, Cao Z, Goeddel D. NF-kappaB -inducing kinase activates<br />
IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci USA<br />
1998;95:3792-3797<br />
5. Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita<br />
H, Okumura K. Differential regulation of IkappaB kinase alpha and<br />
beta by two upstream kinases, NF-kappaB-inducing kinase and<br />
mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl<br />
Acad Sci USA 1998;95:3537-3542 (RD0400)
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Anti-TACE (CT) ADAM17<br />
CATALOG No.: 1131<br />
BACKGROUND:<br />
Tumor-necrosis factor-α is a proinflammatory cytokine and<br />
contributes to a variety of inflammatory disease responses<br />
and programmed cell death. TNF-α is synthesized as a 26K<br />
type II membrane-bound precursor that is cleaved by a<br />
convertase to generate secreted 17K mature TNF-α. TNF-α<br />
converting enzyme (TACE) protein was recently purified and<br />
the human and mouse TACE cDNAs were cloned by several<br />
groups separately (1-3). TACE is a membrane-bound<br />
metalloprotease-disintegrin in the family of mammalian<br />
ADAM (for a disintegrin and metalloprotease). TACE also<br />
processes other cell surface proteins, including TNF<br />
receptor, TGFα, the L-selectin adhesion molecule, and<br />
alpha-cleavage of amyloid protein precursor (APP) (4,5).<br />
TACE mRNA is expressed in a variety of human and murine<br />
tissues. TACE was selected as one of the few targets in<br />
cytokine activation by the Eighth International Conference of<br />
the Inflammation Research Association (6)<br />
SOURCE:<br />
Rabbit anti-TACE (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 807 to 823<br />
of human TACE (1,2). This sequence differs from those of<br />
mouse and rat TACE by one amino acid (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of TACE<br />
by Western blot at 1:500 to 1:2000 dilution. HeLa or Jurkat<br />
whole cell lysate can be used as positive control and 80 to<br />
130 kDa bands can be detected, which may represent<br />
mature protein, precursor, and glycosylated TACE. It is<br />
human, mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at -20°C, stable for one year.<br />
Western blot analysis of<br />
TACE in HeLa (1,3) and<br />
Jurkat (2,4) whole cell lysate<br />
in the absence (1,2) or<br />
presence (3,4) of blocking<br />
peptide (<strong>Catalog</strong> no. 1131P)<br />
with anti-TACE (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Black RA, Rauch CT, Kozlosky CJ, et al. A<br />
metalloproteinase disintegrin that releases tumour-necrosis<br />
factor-alpha from cells. Nature 1997;385:729-733<br />
2. Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin<br />
metalloproteinase that processes precursor tumour-necrosis<br />
factor-alpha. Nature 1997;385:733-736<br />
3. Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of<br />
mouse tumor necrosis factor-alpha converting enzyme (TACE)<br />
and partial analysis of its promoter. Gene 1999;233:67-74<br />
4. Peschon JJ, Slack JL, Reddy P, et al. An essential role for<br />
ectodomain shedding in mammalian development. Science<br />
1998;282:1281-4<br />
5. Buxbaum JD, Liu KN, Luo Y, et al. Evidence that tumor<br />
necrosis factor alpha converting enzyme is involved in<br />
regulated alpha-secretase cleavage of the Alzheimer amyloid<br />
protein precursor. J Biol Chem 1998;273:27765-7<br />
6. Giegel DA, Chaplin DD. Targets in cytokine activation.<br />
Agents Actions Suppl 1998;49:1-3 (WD1299)
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Anti- GFRα-1 GDNFRα, RETL1, TrnR1<br />
CATALOG No.: 1133<br />
BACKGROUND:<br />
Glial cell line-derived neurotrophic factor (GDNF) is a potent<br />
survival factor for central and peripheral neurons and is<br />
essential for the development of kidneys and the enteric<br />
nerves system. Physiological responses to GDNF require<br />
the presence of a novel glycosylphosphadidylinositol linked<br />
protein GDNFRα, which is a cell surface receptor for GDNF<br />
(1,2). The cDNAs encoding GDNFRα from human, rat,<br />
chicken and mouse have been cloned recently (1-5).<br />
GDNFRα was also termed Ret ligand 1 (RETL1) or TGF-βrelated<br />
neurotrophic factor receptor 1 (TrnR1) and<br />
nominated as GFRα-1 recently (5-7). GFRα-1 binds GDNF<br />
specifically and mediates activation of the Ret protein<br />
tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK<br />
form a complex to transduce GDNF signal and to mediate<br />
GDNF function.<br />
SOURCE:<br />
Rabbit anti- GFRα-1 polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 369 to 382 of human<br />
GFRα-1 (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of GFRα-<br />
1 by Western blot. The amino acid sequence of<br />
immunogenic peptide is identical to those of mouse and rat<br />
and the antibody recognizes GFRα-1 from human, mouse<br />
and rat origins. This antibody is for research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at -20°C. Stable for<br />
one year at 2-8°C.<br />
REFERENCES:<br />
Western blot analysis of<br />
GFRα-1 in crude<br />
membrane fractions of<br />
human brain (B), liver (L),<br />
kidney (K), and spleen (S),<br />
respectively, with anti-<br />
GFRα−1 at 1:500 dilution.<br />
1. Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z;<br />
Cupples R; Louis JC; Hu S; Altrock BW; Fox GM. GDNF-induced activation<br />
of the Ret protein tyrosien kinase is mediated by GDNFR-α, a novel receptor<br />
for GDNF. Cell, 1996;85:1113-24.<br />
2. Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD;<br />
Gray C; Armanini MP; Pollock RA; Herti F; Phillips HS; Goddard A; Moore<br />
MW; Buj-Bello A;Davies AM; Asai N; Takahashi M; Vandlen R; Henderson<br />
CE; Rosenthal A. Characterization of a multicomponent receptor for GDNF.<br />
Nature, 1996;382:80-83.<br />
3. Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C, Walus L,<br />
Robinson S, Jaworski G, Wei H, Tizard R, Whitty A, Pepinsky RB, Cate RL.<br />
Glial cell line-derived neurotrophic factor-dependent RET activation can be<br />
mediated by two different cell-surface accessory proteins. Proc Natl Acad<br />
Sci U S. 1997;94:6238-6243<br />
4. Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A,<br />
Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires<br />
a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature<br />
1997;387:721-724<br />
5. Dey,B.K., Wong,Y.W. and Too,H.P. Cloning of a novel murine isoform of<br />
the glial cell line-derived neurotrophic factor receptor. Neuroreport<br />
1998;9:37-42<br />
6. Baloh RH, Tansey MG, Golden JP, Creedon DJ, Heuckeroth RO, Keck<br />
CL, ZimonjicDB, Popescu NC, Johnson EM Jr, Milbrandt J. TrnR2, a novel<br />
receptor that mediates neurturin and GDNF signaling through Ret. Neuron<br />
1997;18:793-802<br />
7. Nomenclature Committee. Nomenclature of GPI-linked receptors for the<br />
GDNF ligand family. GFRα.. Neuron 1997;19:485 (RD1299)
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Anti- GFRα−2, GDNFRβ, RETL2, TrnR2, NTNR-α<br />
CATALOG No.: 1135<br />
BACKGROUND:<br />
Members of the glial cell line-derived neurotrophic factor<br />
(GDNF) family, including GDNF and neurturin (NTN) (1),<br />
play key roles in the control of vertebrate neuron survival<br />
and differentiation. Physiological responses to NTN require<br />
the presence of a novel glycosylphosphadidylinositol linked<br />
protein NTNRα, which is a cell surface receptor for NTN<br />
(2,3). The cDNAs encoding NTNRα from human, rat,<br />
chicken, and mouse have been cloned recently (2-7).<br />
NTNRα was also termed GDNFRβ, Ret ligand 2 (RETL2) or<br />
TGF-β-related neurotrophic factor receptor 2 (TrnR2) (4-7)<br />
and nominated as GFRα-2 recently (8). GFRα-2 binds NTN<br />
and mediates activation of RET receptor tyrosine kinase by<br />
both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK<br />
form a complex to transduce NTN signal and to mediate<br />
NTN function.<br />
SOURCE:<br />
Rabbit anti-GFRα-2 polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 377 to 391 of<br />
human GFRα-2. The amino acid sequences of immunogenic<br />
peptide are identical to those of mouse and rat.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of GFRα-<br />
2 by Western blot at 1:1000 to 1:2000 dilution. HeLa whole<br />
cell lysate can be used as positive control and a 52 kDa<br />
band should be detected. It is human, mouse, and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at -20°C. Stable for<br />
one year at 2-8°C.<br />
REFERENCES:<br />
Western blot analysis of GFRα-2<br />
in HeLa total cell lysate with anti-<br />
GFRα-2 at 1:1000 dilution.<br />
1. Kotzbauer PT, Lampe PA, Heuckeroth RO, Golden JP, Creedon<br />
DJ, Johnson EM Jr, lbrandt J. Neurturin, a relative glial-cell-linederived<br />
neurotrophic factor. Nature 1996;384:467-470<br />
2. Klein RD, Sherman D, Ho WH, Stone D, Bennett GL, Moffat B,<br />
Vandlen R, Simmons L, Gu Q, Hongo JA, Devaux B, Poulsen K,<br />
Armanini M, Nozaki C, Asai N, Goddard A, Phillips H, Henderson<br />
CE, Takahashi M, Rosenthal A. A GPI-link ed protein that interacts<br />
with Ret to form a candidate neurturin receptor. Nature<br />
1997;387:717-721<br />
3. Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal<br />
A, Chinchetru M, Buchman VL, Davies AM. Neurturin<br />
responsiveness requires a GPI-linked receptor and the Ret receptor<br />
tyrosine kinase. Nature 1997;387:721-724<br />
4. Baloh RH, Tansey MG, Golden JP, Creedon DJ, Heuckeroth RO,<br />
Keck CL, Zimonjic DB, Popescu NC, Johnson EM Jr, Milbrandt J.<br />
TrnR2, a novel receptor that mediates neurturin and GDNF<br />
signaling through Ret. Neuron 1997;18:793-802<br />
5. Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C,<br />
Walus L, Robinson S, Jaworski G, Wei H, Tizard R, Whitty A,<br />
Pepinsky RB, Cate RL. Glial cell line-derived neurotrophic factordependent<br />
RET activation can be mediated by two different cellsurface<br />
accessory proteins. Proc Natl Acad Sci USA 1997;94:6238-<br />
6243<br />
6. Suvanto P, Wartiovaara K, Lindahl M, Arumae U, Moshnyakov<br />
M, Horelli-Kuitunen N, Airaksinen MS, Palotie A, Sariola H, Saarma<br />
M. Cloning, mRNA distribution and chromosomal localisation of the<br />
gene for glial cell line-derived neurotrophic factor receptor beta, a<br />
homologue to GDNFR-alpha. Hum Mol Genet 1997;6:1267-1273<br />
7. Wang CY, Ni J, Jiang H, Hsu TA, Dugich-Djordjevic M, Feng L,<br />
Zhang M, Mei L, Gentz R, Lu B. Cloning and characterization of<br />
glial cell line-derived neurotrophic factor receptor-B: a novel<br />
receptor for members of glial cell line-derived neurotrophic factor<br />
family of neurotrophic factors. Neuroscience 1998 Mar;83(1):7-14<br />
8. Nomenclature Committee. Nomenclature of GPI-linked receptors<br />
for the GDNF ligand family. GFRα. Neuron 1997;19:485 (RD1299)
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Anti- GFRα-3, GDNFRγ, RETL2, TrnR2, NTNR-α<br />
CATALOG No.: 1137<br />
BACKGROUND:<br />
Members of the glial cell line-derived neurotrophic factor<br />
(GDNF) family including GDNF and neurturin (NTN) play<br />
essential roles in the control of vertebrate neuron survival<br />
and differentiation. A new member of the GDNF family was<br />
recently identified and designated persephin (1).<br />
Physiological responses to these neurotrophic factors<br />
require two receptor subunits, the novel<br />
glycosylphosphadidylinositol linked protein GFRα and Ret<br />
receptor tyrosine kinase GFRβ (2). Following the findings of<br />
GFRα-1 and -2, a novel receptor in GFRα family was<br />
identified very recently from human and mouse and<br />
designated GFRα-3 (3-7). GFRα-3 binds persephin, thus,<br />
persephin, GFRα-3, and Ret PTK form a complex to<br />
transduce persephin signal and to mediate persephin<br />
function.<br />
SOURCE:<br />
Rabbit anti-GFRα-3 polyclonal antibody was raised against<br />
a peptide corresponding to amino acids from 347 to 360 of<br />
murine GFRα-3 (6).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of GFRα-<br />
3 expression by Western blot at 1:500 to 1:1000 dilution.<br />
Murine kidney, liver or heart tissue lysates can be used as<br />
positive control. It is human mouse, and rat reactive. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at -20°C. Stable for<br />
one year at 2-8°C.<br />
REFERENCES:<br />
Western blot analysis of<br />
GFRα−3 in crude cell<br />
membrane fractions of<br />
murine heart (H), spleen<br />
(S), kidney (K), liver (L),<br />
and brain (B), respectively,<br />
with anti-GFRα−3 at 1:500<br />
dilution.<br />
1. Milbrandt J, de Sauvage FJ, Fahrner TJ, et al. Persephin, a<br />
novel neurotrophic factor related to GDNF and neurturin. Neuron<br />
1998;20:245-253.<br />
2. Nomeclature Committee. Nomeclature of GPI-linked receptors<br />
for the GDNF ligand family. GFRα. Neuron 1997: 19:485.<br />
3. Jing S. Yu Y, Fang M, Hu Z, Holst PL, Boone T, Delaney J.<br />
Schultz H, Zhou R, Fox GM. GFRalpha-2 and GFRalpha-3 are two<br />
new receptors for ligands of the DGNF family. J Biol Chem 1997;<br />
272:33111-33117<br />
4. Baloh RH, Gorodinsky A, Golden JP, Tansey MG, Keck CL,<br />
Popescu NC, Johnson EM Jr, Milbrandt J. GFRalpha3 is an orphan<br />
member of the GDNF/neurturin/persephin receptor family. Proc Natl<br />
Acad Sci USA 1998;95:5801-5806.<br />
5. Worby CA, Vega QC, Chao HH, Seasholtz AF, Thompson RC,<br />
Dixon JE. Identification and characterization of GFRalpha-3, a<br />
novel Co-receptor belonging to the glial cell line-derived<br />
neurotrophic receptor family. J Biol Chem 1998;273:3502-3508.<br />
6 Naveilhan P, Baudet C, Mikaels A, Shen L, Westphal H, Ernfors<br />
P. Expression and regulation of GFRalpha3, a glial cell line-derived<br />
neurotrophic factor family receptor. Proc Natl Acad Sci USA<br />
1998;95:1295-1300.<br />
7. Nomoto,S., Ito,S., Yang,L.X. and Kiuchi,K. Molecular cloning and<br />
expression analysis of GFR alpha-3, a novel cDNA related to<br />
GDNFR alpha and NTNR alpha. Biochem. Biophys. Res. Commun.<br />
1998;244:849-853. (RD1299)
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Anti-DR4 (CT) TRAIL-R1<br />
CATALOG No.: 1139<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand in the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. A<br />
novel death domain containing receptor was recently<br />
identified and designated DR4 (for death receptor 4) 1 . The<br />
ligand for this novel death receptor has been identified and<br />
termed TRAIL 2,3 , which is a new member in the TNF family.<br />
DR4 is also called TRAIL receptor-1 (TRAIL-R1) 4 . DR4 is<br />
expressed in most of human tissues including spleen,<br />
peripheral blood leukocytes, small intestine and thymus.<br />
Like TNFR1, Fas and DR3, DR4 mediates apoptosis and<br />
NF-κB activation in many tissues and cells.<br />
SOURCE:<br />
Rabbit anti-DR4 (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 427 to 445 of<br />
human DR4 mature protein.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DR4<br />
expression by Western blot at 1:500 to 1:1000 dilution. HeLa<br />
whole cell lysate can be used as positive control and a 57<br />
kDa band should be detected. It is human, mouse, and rat<br />
reactive and has no cross reaction to DR5. For research use<br />
only.<br />
Western blot analysis of DR4<br />
in HeLa total cell lysate with<br />
anti-DR4 (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner<br />
R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL.<br />
Science; 1997;276:111-113<br />
2. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl<br />
JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al.<br />
Identification and characterization of a new member of the TNF<br />
family that induces apoptosis. Immunity 1995;3:673-682<br />
3. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A;<br />
Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member<br />
of the tumor necrosis factor cytokine family. J. Biol. Chem.<br />
1996;271:12687-90<br />
4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K,<br />
Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2<br />
(DR5) signal FADD-dependent apoptosis and activate NF-κB.<br />
Immunity 1997;7:831-836<br />
(RD1299)<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DFF45 (CT)<br />
CATALOG No.: 1141<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A human 45 kDa<br />
DNA fragmentation factor (DFF45) was identified recently<br />
which was cleaved by caspase-3 during apoptosis (1).<br />
Mouse homologue of human DFF45 was identified as a<br />
DNase inhibitor designated ICAD (2,3). Upon cleavage of<br />
DFF45/ICAD, a caspase activated deoxyribonuclease<br />
(DFF40/CAD) is released and activated and eventually<br />
causes the degradation of DNA in the nuclei (2-5).<br />
Therefore, the cleavage of DFF45/ICAD, which causes<br />
DFF40/CAD activation and DNA degradation, is the hallmark<br />
of apoptotic cell death.<br />
SOURCE:<br />
Rabbit anti-DFF45 (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 313 to 331<br />
of human DFF45 (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DFF45<br />
and one of the cleaved fragments of DFF45 by Western blot<br />
(6) at 1:1000 to 1:2000 dilution and for immunoprecipitation.<br />
HeLa whole cell lysate can be used as positive control and a<br />
45 kDa band can be detected in non-apoptotic cells. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of<br />
DFF45 in HeLa (H), Jurkat<br />
(J), A431 (A), and K562 (K)<br />
whole cell lysate with anti-<br />
DFF45 (CT) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein<br />
that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H,<br />
Nagata S. Molecular cloning and characterization of human<br />
caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8<br />
6. Tang D, Kidd VJ. Cleavage of DFF-45/ICAD by multiple<br />
caspases is essential for its function during apoptosis. J Biol Chem<br />
1998;273:28549-52<br />
(RD1299)
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DFF45/35 (NT)<br />
CATALOG No.: 1148<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A human 45 kDa<br />
DNA fragmentation factor (DFF45) was identified recently<br />
that was cleaved by caspase-3 during apoptosis (1). Mouse<br />
homologue of human DFF45 was identified as a DNase<br />
inhibitor designated ICAD (2,3). DFF45/ICAD have short<br />
forms that were termed DFF35 and ICADs, respectively,<br />
(2,4). Upon cleavage of DFF45/ICAD, the caspase activated<br />
deoxyribonuclease (DFF40/CAD) is released and activated<br />
and eventually causes the degradation of DNA in the nuclei<br />
(2-5). Therefore, the cleavage of DFF45/ICAD, which causes<br />
DFF40/CAD activation and DNA degradation, is the hallmark<br />
of apoptotic cell death.<br />
SOURCE:<br />
Rabbit anti-DFF45 (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 2 to 21 of<br />
human DFF45 (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DFF45,<br />
DFF35, and one the cleaved fragment by Western blot at<br />
1:1000 to 1:2000 dilution and for immunohistochemistry.<br />
Jurkat whole cell lysate can be used as positive control and<br />
45 and 35 kDa bands can be detected in non-apoptotic cells.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of<br />
DFF45/35 in HeLa, (H),<br />
K562 (K), Jurkat (J), and<br />
Raji (R) whole cell lysate<br />
with anti-DFF45/35 (NT)<br />
at 1:1000 dilution.<br />
REFERENCES:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
4. Gu J, Dong RP, Zhang C, McLaughlin DF, Wu MX, Schlossman<br />
SF. Functional interaction of DFF35 and DFF45 with caspaseactivated<br />
DNA fragmentation nuclease DFF40. J Biol Chem<br />
1999;274:20759-62<br />
5. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
6. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H,<br />
Nagata S. Molecular cloning + characterization of human caspaseactivated<br />
DNase. Proc Natl Acad Sci USA 1998;95:9123-8<br />
(RD1299)
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Anti-MADD DENN<br />
CATALOG No.: 1150<br />
BACKGROUND:<br />
MAP kinase-activating death domain protein (MADD) was<br />
initially identified as the type 1 tumor necrosis factor receptor<br />
(TNFR1) associated protein though their death domains (1).<br />
Overexpression of MADD activates MAP kinases ERK and<br />
JNK and induces the phosphorylation of cytosolic<br />
phospholipase A2 (1). MADD shares 98% identity with<br />
DENN (for differentially expressed in neoplastic vs. normal<br />
cells), which was recently identified as a substrate for c-jun<br />
N-terminal kinase 3 (JNK3) (2, 3). MADD has greater than<br />
94% overall identity to a GDP/GTP exchange protein Rab3-<br />
GEP (4, 5). MADD is 87% identical to KIAA0358, a brain<br />
protein of unknown function (4). Identification of MADD as a<br />
component of the TNFR1 signaling complex and the<br />
similarity between MADD and Rab3-GEP provides a<br />
connection between TNFR1 activation and downstream<br />
MAP kinase activity through a guanine-nucleotide exchange<br />
protein.<br />
SOURCE:<br />
Rabbit anti-MADD polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 1570 to 1588 of<br />
human MADD (1). The sequence of immunogenic peptide is<br />
identical to that of DENN and differs by one amino acid with<br />
rat GDP/GTP exchange protein Rab3-GEP (2,5).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of MADD<br />
by Western blot at 1:250 to 1:500 dilution. Whole cell lysate<br />
from HeLa or NIH3T3 cells can be used as positive control<br />
and 200 to 220 kDa bands should be detected. It is human<br />
and mouse reactive. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at -20°C. Stable for<br />
one year at 2-8°C.<br />
Western blot analysis<br />
of MADD in whole cell<br />
lysates from the<br />
indicated cell lines with<br />
anti-MADD at 1:250<br />
dilution.<br />
REFERENCES:<br />
1. Schievella AR, Chen JH, Graham JR, Lin LL. MADD, a novel<br />
death domain protein that interacts with the type 1 tumor necrosis<br />
factor receptor and activates mitogen-activated protein kinase. J<br />
Biol Chem 1997;272:12069-12075<br />
2. Chow VT, Lee SS. DENN, a novel human gene differentially<br />
expressed in normal and neoplastic cells. DNA Seq 1996;6:263-<br />
273<br />
3. Zhang Y, Zhou L, Miller CA. A splicing variant of a death<br />
domain protein that is regulated by a mitogen-activated kinase is a<br />
substrate for c-Jun N-terminal kinase in the human central nervous<br />
system. Proc Natl Acad Sci U S A 1998;95:2586-2591<br />
4. Brown TL and Howe PH. MADD is highly homologous to a Rab3<br />
guanine-nucleotide exchange protein (Rab3-GEP). Curr Biol<br />
1998;8:R191<br />
5. Wada M, Nakanishi H, Satoh A, Hirano H, Obaishi H, Matsuura<br />
Y, Takai Y. Isolation and characterization of a GDP/GTP exchange<br />
protein specific for the Rab3 subfamily small G proteins. J Biol<br />
Chem 1997;272:3875-3878.<br />
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Anti-ASK1 (CT) MAPKKK5<br />
CATALOG No.: 1151<br />
BACKGROUND:<br />
Mitogen-activated protein (MAP) kinase cascades are<br />
activated in response to various extracellular stimuli,<br />
including cytokines, growth factors and environmental<br />
stresses. A novel MAP kinase kinase kinase (MAPKKK) was<br />
recently identified and designated ASK1 (for apoptosis<br />
signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1<br />
activated two different subgroups of MAPKK, MKK4 and<br />
MKK6, which in turn activated c-Jun N-terminal kinase (JNK)<br />
and p38 MAP kinase, respectively. ASK1/MAPKKK5 is<br />
activated by TNFR and Fas through the interaction with<br />
members of the TRAF family and Fas-associated protein<br />
Daxx. Overexpression of ASK1 induced apoptotic cell death,<br />
and a catalytically inactive form of ASK1 inhibited TNF-αinduced<br />
apoptosis. ASK1 is expressed in variety of human<br />
and mouse tissues.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year<br />
Western blot analysis of<br />
ASK1 in SW1353 whole cell<br />
lysate with anti-ASK1 at<br />
1:500 dilution.<br />
REFERENCES:<br />
1. Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T,<br />
Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of<br />
apoptosis by ASK1, a mammalian MAPKKK that activates<br />
SAPK/JNK and p38 signaling pathways. Science 1997;275:90-4<br />
SOURCE:<br />
Rabbit anti-ASK1 polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 1356 to 1375 of<br />
human ASK1 (1). This sequence is different from that of<br />
mouse by last two amino acids (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of ASK1<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from SW1353 can be used as positive control and a 155<br />
kDa band can be detected. For research use only.<br />
2. Wang XS, Diener K, Jannuzzi D, Trollinger D, Tan TH,<br />
Lichenstein H, Zukowski M, Yao Z. Molecular cloning and<br />
characterization of a novel protein kinase with a catalytic domain<br />
homologous to mitogen-activated protein kinase kinase kinase. J<br />
Biol Chem 1996;271:31607-11<br />
3. Tobiume K, Inage T, Takeda K, Enomoto S, Miyazono K, Ichijo<br />
H. Molecular cloning and characterization of the mouse apoptosis<br />
signal-regulating kinase 1. Biochem Biophys Res Commun<br />
1997;239:905-10<br />
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techsupport@prosci-inc.com<br />
Anti-mFLIP (CT) CASHα<br />
CATALOG No.: 1156<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain (DD) containing<br />
adapter molecules and members of the ICE/CED-3 protease<br />
family. Caspases-8 (FLICE) and -10 (FLICE2) are two<br />
pivotal members in the ICE/CED-3 protease family. FLICEinhibitory<br />
proteins were identified in virus and human and<br />
designated v-FLIPs and FLIP respectively (1,2). The human<br />
FLIP was also cloned by several labs independently and<br />
termed Casper, I-FLICE, FLAME-1, CASH, CLARP and<br />
MRIT (3-8). FLIP contains two death effector domains<br />
(DEDs) and a caspase-like domain. FLIP interacts with<br />
adapter protein FADD and caspase-8 and –10, and potently<br />
inhibits apoptosis induced by all known death receptors<br />
CD95, DR3, TRAIL-R and TNFR1.<br />
SOURCE:<br />
Rabbit anti-mFLIP polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 449 to 465 of mouse<br />
FLIP L /CASHα (2,6).<br />
APPLICATION:<br />
This polyclonal antibody can be used for the detection of<br />
FLIP by Western blot at 1:500 to 1:1000 dilution. NIH/3T3<br />
whole cell lysate can be used as positive control and a 55<br />
kDa band can be detected. FLIP has short form (FLIP S ) and<br />
long form (FLIP L ) and this antibody recognizes the FLIP L<br />
only. This antibody is for research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at -20°C. Stable for<br />
one year at 2-8°C.<br />
Western blot analysis of mFLIP<br />
in NIH/3T3 whole cell lysate<br />
with anti-mFLIP (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F,<br />
Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH,<br />
Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent<br />
apoptosis induced by death receptors. Nature 1997;386:517-521.<br />
2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V,<br />
Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE,<br />
Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature<br />
1997;388:190-195.<br />
3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and caspase-related<br />
inducer of apoptosis. Immunity 1997;6:751-763.<br />
4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of<br />
tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem<br />
1997;272:17255-17257.<br />
5. Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang Y,<br />
Fernandes -Alnemri T, Croce CM, Litwack G, Tomaselli KJ, Armstrong RC,<br />
Alnemri ES. FLAME-1, a novel FADD-like anti-apoptotic molecule that<br />
regulates Fas/TNFR1-induced apoptosis. J Biol Chem 1997;272:18542-<br />
18545.<br />
6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM,<br />
Wallach D. CASH, a novel caspase homologue with death effector domains.<br />
J Biol Chem 1997;272:19641-19644.<br />
7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death effector<br />
domain-containing protein interacts with caspase-8 and regulates apoptosis.<br />
Proc Natl Acad Sci USA 1997;94:10717-10722.<br />
8. Han DK, Chaudhary PM, Wright ME, Friedman C, Trask BJ, Riedel RT,<br />
Baskin DG, Schwartz SM, Hood L. MRIT, a novel death-effector domaincontaining<br />
protein, interacts with caspases and BclXL and initiates cell death.<br />
Proc Natl Acad Sci USA 1997;94:11333-11338.<br />
9. Wallach D. Apoptosis. Placing death under control. Nature 1997;388:123.<br />
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Anti-DR3 (ED) Wsl-1, Apo-3, TRAMP, LARD<br />
CATALOG No.: 1158<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand of the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. A<br />
novel cell death receptor was recently identified by several<br />
groups independently and designated DR3, Wsl-1, Apo-3,<br />
TRAMP and LARD 1-5 . The ligand for this novel death<br />
receptor has been defined as TWEAK, also termed Apo3L.<br />
DR3 is highly expressed in the tissues enriched in<br />
lymphocytes including PBL, thymus and spleen. Like<br />
TNFR1, DR3 induces apoptosis and NF-κB activation.<br />
SOURCE:<br />
Rabbit anti-DR3 (ED) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 59 to 77 in<br />
extracellular domain (ED) of human DR3 precusor 1,2 .<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DR3<br />
expression by Western blot at 1:500 to 1:1000 dilution.<br />
Jurkat total cell lysate can be used as positive control and a<br />
59 kDa band should be detected. It is human and mouse<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of DR3<br />
in Jurkat total cell lysate with<br />
anti-DR3 (ED) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan<br />
DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996;274:990-2.<br />
2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT;<br />
Grinham CJ; Brown R; Farrow SN. Nature, 1996;384:372-5.<br />
3. Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL;<br />
Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996;6:1669-76.<br />
4. Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V; Thome<br />
M; Bornand T; Hahne M; Schroter M; Becker K; et al. Immunity,<br />
1997;6:79-88.<br />
5. Screaton GR; Xu XN; Olsen AL; Cowper AE; Tan R; McMichael<br />
AJ; Bell JI. Proc. Nat. Acad. Sci. USA. 1997;94:4615-9.<br />
(RD1299)
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-FLIP (NT) Casper, I-FLICE, FLAME-1, CASH, CLARP<br />
CATALOG No.: 1159<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain (DD)- containing<br />
adapter molecules and members of the ICE/CED-3 protease<br />
family. Caspases-8 (FLICE) and -10 (FLICE2) are two<br />
pivotal members in the ICE/CED-3 protease family. FLICEinhibitory<br />
proteins were identified in virus and human and<br />
designated v-FLIPs and FLIP, respectively 1,2 . The human<br />
FLIP was also cloned by several labs independently and<br />
termed Casper, I-FLICE, FLAME-1, CASH and CLARP 3-7 .<br />
FLIP contains two death effector domains (DEDs) and a<br />
caspase-like domain. FLIP interacts with adapter protein<br />
FADD and caspase-8 and –10, and potently inhibits<br />
apoptosis induced by all known death receptors. Four splice<br />
variants of c-FLIPs have been identified and termed FLIPα,<br />
β, γ, and δ, respectively 8 .<br />
SOURCE:<br />
Rabbit anti-FLIP (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 2 to 18 of<br />
human FLIP 2 . The sequence is identical in all FLIP splice<br />
variants.<br />
APPLICATION:<br />
This polyclonal antibody can be used for Western blot at<br />
1:500 to 1:1000 dilution. It recognizes all FLIP splice<br />
variants including FLIPα, β, γ, and δ and is human, mouse<br />
and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
REFERENCES:<br />
1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E,<br />
Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C,<br />
Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins<br />
(FLIPs) prevent apoptosis induced by death receptors. Nature<br />
1997;386:517-521<br />
2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner<br />
V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D,<br />
French LE, Tschopp J. Inhibition of death receptor signals by<br />
cellular FLIP. Nature 1997;388:190-195<br />
3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and<br />
caspase-related inducer of apoptosis. Immunity 1997;6:751-763<br />
4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel<br />
inhibitor of tumor necrosis factor receptor-1- and CD-95-induced<br />
apoptosis. J Biol Chem 1997;272:17255-17257<br />
5. Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang<br />
Y, Fernandes-Alnemri T, Croce CM, Litwack G, Tomaselli KJ,<br />
Armstrong RC, Alnemri ES. FLAME-1, a novel FADD-like antiapoptotic<br />
molecule that regulates Fas/TNFR1-induced apoptosis. J<br />
Biol Chem 1997;272:18542-18545<br />
6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE,<br />
Brodianskii VM, Wallach D. CASH, a novel caspase homologue<br />
with death effector domains. J Biol Chem 1997;272:19641-19644<br />
7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death<br />
effector domain-containing protein interacts with caspase-8 and<br />
regulates apoptosis. Proc Natl Acad Sci USA 1997;94:10717-10722<br />
8. Wallach D. Apoptosis. Placing death under control. Nature<br />
1997;388:123 (RD1299).
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-FLIPα (CT) Casper, I-FLICE, FLAME-1, CASH, CLARP<br />
CATALOG No.: 1161<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain (DD)- containing<br />
adapter molecules and members of the ICE/CED-3 protease<br />
family. Caspases-8 (FLICE) and -10 (FLICE2) are two<br />
pivotal members in the ICE/CED-3 protease family. FLICEinhibitory<br />
proteins were identified in virus and human and<br />
designated v-FLIPs and FLIP, respectively 1,2 . The human<br />
FLIP was also cloned by several labs independently and<br />
termed Casper, I-FLICE, FLAME-1, CASH and CLARP 3-7 .<br />
FLIP contains two death effector domains (DEDs) and a<br />
caspase-like domain. FLIP interacts with adapter protein<br />
FADD and caspase-8 and –10, and potently inhibits<br />
apoptosis induced by all known death receptors. Four splice<br />
variants of c-FLIPs have been identified and termed FLIPα,<br />
β, γ, and δ, respectively 8 .<br />
SOURCE:<br />
Rabbit anti-FLIP (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 447 to 464 at C-<br />
terminus of human FLIPα/FLIP L form 2 .<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of FLIP<br />
by Western blot at 1:500 to 1:2000 dilution. HeLa whole cell<br />
lysate can be used as positive control and a 55 kDa band<br />
can be detected. It is human, mouse and rat reactive. This<br />
antibody recognizes the FLIPα only. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of<br />
FLIP in HeLa (H), Jurkat (J),<br />
and K562 (K) whole cell<br />
lysate with anti-FLIP (CT) at<br />
1:1000 dilution.<br />
REFERENCES:<br />
1. Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory<br />
proteins (FLIPs) prevent apoptosis induced by death receptors.<br />
Nature 1997;386:517-521<br />
2. Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor<br />
signals by cellular FLIP. Nature 1997;388:190-195<br />
3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and<br />
caspase-related inducer of apoptosis. Immunity 1997;6:751-763<br />
4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel<br />
inhibitor of tumor necrosis factor receptor-1- and CD-95-induced<br />
apoptosis. J Biol Chem 1997;272:17255-17257<br />
5. Srinivasula SM, Ahmad M, Ottilie S, et al.. FLAME-1, a novel<br />
FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-<br />
induced apoptosis. J Biol Chem 1997;272:18542-18545<br />
6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE,<br />
Brodianskii VM, Wallach D. CASH, a novel caspase homologue<br />
with death effector domains. J Biol Chem 1997;272:19641-19644<br />
7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death<br />
effector domain-containing protein interacts with caspase-8 and<br />
regulates apoptosis. Proc Natl Acad Sci USA 1997;94:10717-10722<br />
8. Wallach D. Apoptosis. Placing death under control. Nature<br />
1997;388:123 (RD1299)
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Poway, CA 92064<br />
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techsupport@prosci-inc.com<br />
Anti-Daxx (CT)<br />
CATALOG No.: 1163<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand of the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. Cell<br />
death signals are transduced by death domain (DD)-<br />
containing adapter molecules and members of the ICE/CED-<br />
3 protease family. A novel DD-containing molecule was<br />
recently cloned from mouse, human and monkey and<br />
designated Daxx 1,2 . Daxx binds specifically to the Fas death<br />
domain and enhances Fas induced apoptosis and activates<br />
the Jun N-terminal kinase (JNK) pathway. Daxx is widely<br />
expressed in fetal and adult human and mouse tissues<br />
indicating its important function in Fas signaling pathways 1,2 .<br />
SOURCE:<br />
Rabbit anti-Daxx (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 722 to 740<br />
at C-terminus of human Daxx 1,2 .<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Daxx<br />
by Western blot at 1:500 to 1:1000 dilution. HeLa whole cell<br />
lysate can be used as positive control and a 120 kDa major<br />
band can be detected. The sequences of immunogenic<br />
peptide are identical among human, monkey and mouse<br />
origins of Daxx 1,2 , and the antibody recognizes Daxx from<br />
all these species. For research use only.<br />
Western blot analysis of Daxx in<br />
HeLa total cell lysate with anti-<br />
Daxx (CT) at 1:1000 dilution.<br />
REFERENCES:<br />
1. Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel<br />
Fas-binding protein that activates JNK and apoptosis. Cell<br />
1997;89:1067-1076<br />
2. Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd.<br />
Cloning and expression of primate Daxx cDNAs and mapping of<br />
the human gene to chromosome 6p21.3 in the MHC region. DNA<br />
Cell Biol 1997;16:1289-1298<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DR3 (NT) Wsl-1, Apo-3, TRAMP, LARD<br />
CATALOG No.: 1166<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand of the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. A novel<br />
cell death receptor was recently identified by several groups<br />
independently and designated DR3, Wsl-1, Apo-3, TRAMP<br />
and LARD 1-5 . The ligand for this novel death receptor has<br />
been defined as TWEAK, also termed Apo3L. DR3 is highly<br />
expressed in the tissues enriched in lymphocytes including<br />
PBL, thymus and spleen. Like TNFR1, DR3 induces<br />
apoptosis and NF-κB activation.<br />
SOURCE:<br />
Rabbit anti-DR3 (NT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 25 to 40 of human<br />
DR3 precusor 1,2 .<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DR3 by<br />
Western blot at 1:500 to 1:1000 dilution. Jurkat whole cell<br />
lysate can be used as positive control and a 59 kDa band<br />
can be detected. It is human and mouse reactive and has no<br />
cross reaction to other death receptors. For research use<br />
only.<br />
STORAGE: It is supplied as purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
Western blot analysis<br />
of DR3 in Jurkat total<br />
cell lysate with anti-<br />
DR3 (NT) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan<br />
DR; Xing L; Gentz R; Ni J; Dixit VM. Science, 1996;274:990-2.<br />
2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT;<br />
Grinham CJ; Brown R; Farrow SN. Nature, 1996;384:372-5.<br />
3. Marsters SA; Sheridan JP; Donahue CJ; Pitti RM; Gray CL;<br />
Goddard AD; Bauer KD; Ashkenazi A. Curr Biol, 1996;6:1669-76.<br />
4. Bodmer JL; Burns K; Schneider P; Hofmann K; Steiner V;<br />
Thome M; Bornand T; Hahne M; Schroter M; Becker K; et al.<br />
Immunity, 1997;6:79-88.<br />
5. Screaton GR; Xu XN; Olsen AL; Cowper AE; Tan R; McMichael<br />
AJ; Bell JI. Proc. Nat. Acad. Sci. USA. 1997;94:4615-9.<br />
(RD1299)
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Anti-DR4 (NT) TRAIL-R1<br />
CATALOG No.: 1167<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, occurs during normal<br />
cellular differentiation and development of multicellular<br />
organisms. Apoptosis is induced by certain cytokines<br />
including TNF and Fas ligand in the TNF family through their<br />
death domain containing receptors, TNFR1 and Fas. A novel<br />
death domain containing receptor was recently identified and<br />
designated DR4 (for death receptor 4) 1 . The ligand for this<br />
novel death receptor has been identified and termed<br />
TRAIL 2,3 , which is a new member in the TNF family. DR4 is<br />
also called TRAIL receptor-1 (TRAIL-R1) 4 . DR4 is expressed<br />
in most of human tissues including spleen, peripheral blood<br />
leukocytes, small intestine and thymus. Like TNFR1, Fas<br />
and DR3, DR4 mediates apoptosis and NF-κB activation in<br />
many tissues and cells.<br />
SOURCE:<br />
Rabbit anti-DR4 (NT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acid 1 to 20 of human<br />
DR4 mature protein.<br />
STORAGE:<br />
Supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of DR4<br />
in HeLa (H), K562 (K), and<br />
Jurkat (J) whole cell lysate<br />
with anti-DR4 (NT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J;<br />
Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science;<br />
1997;276:111-113<br />
2. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK,<br />
Sutherland GR, Smith TD, Rauch C, Smith CA, et al. Identification and<br />
characterization of a new member of the TNF family that induces apoptosis.<br />
Immunity 1995;3:673-682<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DR4 by<br />
Western blot at 1:500 to 1:1000 dilution. HeLa or Jurkat<br />
whole cell lysate can be used as positive control and a 57<br />
kDa band can be detected. For research use only.<br />
3. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A.<br />
Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis<br />
factor cytokine family. J. Biol. Chem. 1996;271:12687-90<br />
4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T,<br />
Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADDdependent<br />
apoptosis and activate NF-κB. Immunity 1997;7:831-836<br />
(RD1299)
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Anti-Bonzo (CT) STRL33, TYMSTR<br />
CATALOG NO.: 1170<br />
BACKGROUND:<br />
Human immunodeficiency virus (HIV) and simian<br />
immunodeficiency virus (SIV) require coreceptors, in addition<br />
to CD4, to infect target cells. Some G protein-coupled<br />
receptors including CCR5, CXCR4, CCR3, and CCR2b in<br />
the chemokine receptor family have been identified as HIV<br />
coreceptors. An orphan G protein-coupled receptor was<br />
recently cloned and designated Bonzo, STRL33 and<br />
TYMSTR, and identified as HIV and SIV coreceptor (1-4).<br />
Bonzo/STRL33 is used by SIV, HIV-2 and HIV-1. The<br />
messenger RNA of Bonzo/STRL33 is expressed in lymphoid<br />
tissues and activated peripheral blood lymphocytes.<br />
SOURCE:<br />
Rabbit anti-Bonzo polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 319 to 338 of human<br />
origin (1,2). The sequence of this peptide is identical to those<br />
of macaque and African green monkey.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Bonzo<br />
by Western blot at 1:1000 dilution. Whole cell lysate from<br />
SW1353 cells can be used as positive control. This antibody<br />
is for research use only.<br />
STORAGE:<br />
It is supplied as purified IgG fraction, 100 µg in 200 µl of<br />
PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
Western blot analysis of<br />
Bonzo in SW1353 total<br />
cells lysate with anti-<br />
Bonzo (CT) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR.<br />
Expression cloning of new receptors used by simian and human<br />
immunodeficiency viruses. Nature 1997;388:296-300<br />
2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber<br />
JM. STRL33, A novel chemokine receptor-like protein, functions as<br />
a fusion cofactor for both macrophage-tropic and T cell line-tropic<br />
HIV-1. J Exp Med 1997;185:2015-23<br />
3. Alkhatib G, Liao F, Berger EA, Farber JM, Peden KW. A new<br />
SIV co-receptor, STRL33. Nature 1997;388:238<br />
4. Loetscher M, Amara A, Oberlin E, Brass N, Legler D, Loetscher<br />
P, D'Apuzzo M, Meese E, Rousset D, Virelizier JL, Baggiolini M,<br />
Arenzana-Seisdedos F, Moser B. TYMSTR, a putative chemokine<br />
receptor selectively expressed in activated T cells, exhibits HIV -1<br />
coreceptor function. Curr Biol 1997;7:652-60 (RD1299)
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Anti-ICAD (NT)<br />
CATALOG No.: 2001<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A human DNA<br />
fragmentation factor (DFF) was identified recently which is<br />
cleaved by caspase-3 during apoptosis (1). Mouse<br />
homologue of human DFF was identified as a DNase<br />
inhibitor designated ICAD, for inhibitor of caspase-activated<br />
DNase (2,3). Upon cleavage of DFF/ICAD, a caspase<br />
activated deoxyribonuclease (CAD) is released and<br />
activated and eventually causes the degradation of DNA in<br />
the nuclei (2,3). Therefore, the cleavage of CAD inhibitor<br />
molecule DFF/ICAD, which causes DNase activation and<br />
DNA degradation, is the hallmark of apoptotic cell death (4).<br />
SOURCE:<br />
Rabbit anti-ICAD (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 2 to 21 of<br />
mouse ICAD (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of of<br />
ICAD by Western blot at 1:500 to 1:1000 dilution. Murine<br />
lung tissue lysate can be used as positive control and a 45<br />
kDa band cad be detected. This antibody is for research use<br />
only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
Western blot analysis of<br />
ICAD in murine lung<br />
tissue lysate with anti-<br />
ICAD (NT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
4. Wyllie A. Apoptosis. An endonuclease at last. Nature<br />
1998;39120-21(RD1299)
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Anti-ICAD (CT)<br />
CATALOG No.: 2003<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A human DNA<br />
fragmentation factor (DFF) was identified recently which was<br />
cleaved by caspase-3 during apoptosis (1). Mouse<br />
homologue of human DFF was identified as a DNase<br />
inhibitor designated ICAD, for inhibitor of caspase-activated<br />
DNase (2,3). Upon cleavage of DFF/ICAD, a caspase<br />
activated deoxyribonuclease (CAD) is released and<br />
activated and eventually causes the degradation of DNA in<br />
the nuclei (2,3). Therefore, the cleavage of CAD inhibitor<br />
molecule DFF/ICAD, which causes DNase activation and<br />
DNA degradation, is the hallmark of apoptotic cell death (4).<br />
SOURCE:<br />
Rabbit anti-ICAD (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 312 to 331<br />
of mouse ICAD (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of ICAD<br />
by Western blot at 1:500 to 1:2000 dilution. Murine lung<br />
tissue lysate can be used as positive control and a 45 kDa<br />
band can be detected. This antibody is for research use<br />
only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
Western blot analysis<br />
of ICAD in murine lung<br />
(L), brain (B), liver (L),<br />
and kidney tissue<br />
lysate with anti-ICAD<br />
(CT) at 1:1000 dilution.<br />
REFERENCES:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
4. Wyllie A. Apoptosis. An endonuclease at last. Nature<br />
1998;39120-21<br />
(RD1299)
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Anti-CAD (I17)<br />
CATALOG No.: 2007<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A mouse DNase<br />
that causes DNA fragmentation was identified recently and<br />
designated CAD (for caspase activated deoxyribonuclease)<br />
(1,2). The human homologue of mouse CAD was more<br />
recently identified by two groups independently and termed<br />
CPAN and DFF40 (3,4). Human DFF45 and its mouse<br />
homologue ICAD are the inhibitors of CPAN/DFF40 and<br />
CAD, respectively (5). Upon cleavage of DFF45/ICAD by<br />
activated caspase, DFF40/CAD is released and activated<br />
and eventually causes the degradation of DNA in the nuclei<br />
(1-4). Activation of CAD/DFF40, which causes DNA<br />
degradation, is the hallmark of apoptotic cell death.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
Western blot analysis of<br />
CAD in murine kidney<br />
tissue lysate with anti-CAD<br />
(I17) at 1:500 dilution.<br />
REFERENCES:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The<br />
SOURCE:<br />
Rabbit anti-CAD (I17) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 205 to 222 of murine<br />
CAD (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of CAD<br />
by Western blot at 1:500 to 1:1000 dilution. Murine kidney<br />
tissue lysate can be used as positive control and a 40 kDa<br />
band should be detected. It is mouse and rat reactive. For<br />
research use only.<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L,<br />
Williams LT. CPAN, a human nuclease regulated by the caspasesensitive<br />
inhibitor DFF45. Curr Biol 1998;8:537-40<br />
5. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
(RD1299)
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Anti-CAD (CT)<br />
CATALOG No.: 2011<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A mouse DNase<br />
that causes DNA fragmentation was identified recently and<br />
designated CAD (for caspase activated deoxyribonuclease)<br />
(1,2). The human homologue of mouse CAD was more<br />
recently identified by two groups independently and termed<br />
CPAN and DFF40 (3,4). Human DFF45 and its mouse<br />
homologue ICAD are the inhibitors of CPAN/DFF40 and<br />
CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD<br />
by activated caspase, DFF40/CAD is released and activated<br />
and eventually causes the degradation of DNA in the nuclei<br />
(1-4). Activation of CAD/DFF40, which causes DNA<br />
degradation, is the hallmark of apoptotic cell death.<br />
SOURCE:<br />
Rabbit anti-CAD (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 314 to 329 of murine<br />
CAD (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of CAD<br />
by Western blot at 1:500 to 1:1000 dilution. Tissue lysate<br />
from murine lung or kidney can be used as positive control<br />
and a 40 kDa band should be detected. For research use<br />
only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
Western blot analysis of<br />
CAD in murine lung (L) and<br />
kidney (K) tissue lysates<br />
with anti-CAD (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L,<br />
Williams LT. CPAN, a human nuclease regulated by the caspasesensitive<br />
inhibitor DFF45. Curr Biol 1998;8:537-40<br />
5. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
(RD1299)
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Anti-Apaf-1 (NT)<br />
CATALOG No.: 2013<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
The mammalian homologues of the key cell death gene<br />
CED-4 in C. elegans has been identified recently from<br />
human and mouse and designated Apaf-1 (for apoptosis<br />
protease-activating factor 1) (1,2). Apaf-1 binds to<br />
cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads<br />
to caspase-9 activation. Activated caspase-9 in turn cleaves<br />
and activates caspase-3 that is one of the key proteases,<br />
being responsible for the proteolytic cleavage of many key<br />
proteins in apoptosis (3). Apaf-1 can also associate with<br />
caspase-4 and caspase-8 (4). Apaf-1 is ubiquitously<br />
expressed in human tissues (1).<br />
SOURCE:<br />
Rabbit anti-Apaf-1 (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 12 to 28 of<br />
human Apaf-1 (1). The sequences of the immunogenic<br />
peptide are identical between human and mouse (1,2)<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
REFERENCES:<br />
1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human<br />
protein homologous to C. elegans CED-4, participates in<br />
cytochrome c-dependent activation of caspase-3. Cell<br />
1997;90:405-13<br />
2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P.<br />
Apaf1 (CED-4 homolog) regulates programmed cell death in<br />
mammalian development. Cell 1998;94:727-37<br />
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M,<br />
Alnemri ES, Wang X. Cytochrome c and dATP-dependent<br />
formation ofApaf-1/caspase-9 complex initiates an apoptotic<br />
protease cascade. Cell 1997;91:479-89<br />
4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts<br />
with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.<br />
Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Apaf-1<br />
by Western blot at 1:1000 to 1:2000 dilution. Whole cell<br />
lysate from HeLa cells can be used as positive control and a<br />
130 kDa band should be detected. It is human, mouse, and<br />
rat reactive. This antibody is for research use only.
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Anti-Apaf1 (CT)<br />
CATALOG No.: 2015<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
The mammalian homologous of the key cell death gene<br />
CED-4 in C. elegans was identified recently from human and<br />
mouse and designated Apaf1 for apoptosis proteaseactivating<br />
factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2)<br />
and caspase-9 (Apaf3), which leads to caspase-9 activation.<br />
Activated caspase-9 in turn cleaves and activates caspase-3<br />
that is one of the key proteases, being responsible for the<br />
proteolytic cleavage of many key proteins in apoptosis (3).<br />
Apaf1 can also associate with caspase-4 and caspase-8 (4).<br />
Apaf1 transcript is ubiquitously expressed in human tissues<br />
(1).<br />
SOURCE:<br />
Rabbit anti-Apaf1 (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 1158 to<br />
1177 of human Apaf1 (1). The sequence of the immunogenic<br />
peptide differs from that of murine Apaf1 by one amino acid<br />
(1,2)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Apaf1<br />
by Western blot at 1:500 to 1:2000 dilution. Human heart<br />
tissue lysate can be used as positive control and a 130 kDa<br />
band should be detected. It is human, mouse and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
Apaf1 in human heart<br />
tissue lysate with anti-Apaf1<br />
(2015) at 1:500 dilution in<br />
the absence (A) or<br />
presence (B) of blocking<br />
peptide.<br />
REFERENCES:<br />
1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human<br />
protein homologous to C. elegans CED-4, participates in<br />
cytochrome c-dependent activation of caspase-3. Cell<br />
1997;90:405-13<br />
2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P.<br />
Apaf1 (CED-4 homolog) regulates programmed cell death in<br />
mammalian development. Cell 1998;94:727-37<br />
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M,<br />
Alnemri ES, Wang X. Cytochrome c and dATP-dependent<br />
formation of Apaf-1/caspase-9 complex initiates an apoptotic<br />
protease cascade. Cell 1997;91:479-89<br />
4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts<br />
with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation.<br />
Proc Natl Acad Sci USA 1998;95:4386-91<br />
(WD1299)<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg / 250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2015P).
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Anti-DR5 Apo2, TRAIL-R2, TRICK2, KILLER<br />
CATALOG No.: 2019<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand in the TNF family through their death domain<br />
containing receptors. TRAIL/Apo2L is a new member of the<br />
TNF family. DR4 was recently identified as the receptor for<br />
TRAIL. A novel death domain containing receptor for TRAIL<br />
was more recently identified and designated DR5, Apo2,<br />
TRAIL-R2, TRICK2, or KILLER by several groups<br />
independently (1-7). Like DR4, DR5 transcript is widely<br />
expressed in normal tissues and in many types of tumor<br />
cells. DR5 binds to TRAIL and mediates TRAIL induced cell<br />
death. Overexpression of DR5 induces apoptosis and<br />
activates NF-κB.<br />
SOURCE:<br />
Rabbit anti-DR5 polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 388 to 407 of human<br />
DR5 precursor (1,2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DR5<br />
expression by Western blot at 1:500 to 1:1000 dilution.<br />
Whole cell lysate from HeLa cells can be used as positive<br />
control. It is human and mouse reactive and has no cross<br />
reaction to DR4. For research use only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100 µg<br />
in 200 µl of PBS containing 0.02% sodium azide. Store at -<br />
20°C. Stable for one year at 2-8°C.<br />
Western blot analysis of DR5<br />
in HeLa (H) and K562 (K)<br />
whole cell lysates with anti-<br />
DR5 at 1:500 dilution.<br />
2. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M,<br />
Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI,<br />
Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced<br />
apoptosis by a family of signaling and decoy receptors. Science<br />
1997;277:818-21<br />
3. Walczak H, Degli-Esposti MA, Johnson RS, Smolak PJ, Waugh<br />
JY, Boiani N, Timour MS, Gerhart MJ, Schooley KA, Smith CA,<br />
Goodwin RG, Rauch CT. TRAIL-R2: a novel apoptosis-mediating<br />
receptor for TRAIL. EMBO J 1997;16:5386-97<br />
4. MacFarlane M, Ahmad M, Srinivasula SM, Fernandes-Alnemri<br />
T, Cohen GM, Alnemri ES. Identification and molecular cloning of<br />
two novel receptors for the cytotoxic ligand TRA IL. J Biol Chem<br />
1997;272:25417-20<br />
5. Screaton GR, Mongkolsapaya J, Xu XN, Cowper AE, McMichael<br />
AJ, Bell JI. TRICK2, a new alternatively spliced receptor that<br />
transduces the cytotoxic signal from TRAIL. Curr Biol 1997;7:693-6<br />
6. Wu GS, Burns TF, McDonald ER 3rd, Jiang W, Meng R, Krantz<br />
ID, Kao G, Gan DD, Zhou JY, Muschel R, Hamilton SR, Spinner<br />
NB, Markowitz S, Wu G, el-Deiry WS. KILLER/DR5 is a DNA<br />
damage-inducible p53-regulated death receptor gene. Nat Genet<br />
1997;17:141-3<br />
7. Chaudhary PM, Eby M, Jasmin A, Bookwalter A, Murray J, Hood<br />
L Death receptor 5, a new member of the TNFR family, and DR4<br />
induce FADD-dependent apoptosis and activate the NF-kappaB<br />
pathway. Immunity 1997;7:821-30 (RD1299)<br />
REFERENCES:<br />
1. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist<br />
decoy receptor and a death domain-containing receptor for TRAIL.<br />
Science 1997;277:815-8
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Anti-DcR2 (ID) TRAIL-R4, TRUNDD<br />
CATALOG No.: 2021<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand in the TNF family through their death domain<br />
containing receptors. TRAIL/Apo2L is a new member of the<br />
TNF family and induces apoptosis of a variety of tumor cell<br />
lines. DR4 and DR5 are the recently identified functional<br />
receptors for TRAIL, and DcR1/TRID is a decoy receptor (1-<br />
3). Another member of the TRAIL receptor family was more<br />
recently identified and designated DcR2, TRAIL-R4, or<br />
TRUNDD (4-6). DcR2 has an extracellular TRAIL-binding<br />
domain but lacks intracellular death domain and does not<br />
induce apoptosis. Like DR4 and DR5, DcR2 transcript is<br />
widely expressed in normal human tissues. Overexpression<br />
of DcR2 attenuated TRAIL-induced apoptosis.<br />
SOURCE:<br />
Rabbit anti-DcR2 (ID) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 249 to 263 of human<br />
DcR2 precursor (4-6).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DcR2<br />
expression by Western blot at 1:1000 to 1:2000 dilution.<br />
Whole cell lysate from HeLa cells can be used as positive<br />
control. It is human, mouse, and rat reactive. For research<br />
use only.<br />
Western blot analysis of<br />
DcR2 in HeLa whole cell<br />
lysate with anti-DcR2 (ID)<br />
at 1:1000 dilution.<br />
REFERENCES:<br />
1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R;<br />
Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand<br />
TRAIL. Science; 1997;276:111-113<br />
2. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist<br />
decoy receptor and a death domain-containing receptor for TRAIL.<br />
Science 1997;277:815-8<br />
3. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M,<br />
Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI,<br />
Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced<br />
apoptosis by a family of signaling and decoy receptors. Science<br />
1997;277:818-21<br />
4. Marsters SA, Sheridan JP, Pitti RM, Huang A, Skubatch M,<br />
Baldwin D, Yuan J, Gurney A, Goddard AD, Godowski P,<br />
Ashkenazi A. A novel receptor for Apo2L/TRAIL contains a<br />
truncated death domain. Curr Biol 1997;7:1003-6<br />
5. Degli-Esposti MA, Dougall WC, Smolak PJ, Waugh JY, Smith<br />
CA, Goodwin RG. The novel receptor TRAIL-R4 induces NFkappaB<br />
and protects against TRAIL-mediated apoptosis, yet<br />
retains an incomplete death domain. Immunity 1997;7:813-20<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100 µg<br />
in 200 µl of PBS containing 0.02% sodium azide. Store at -<br />
20°C. Stable for one year at 2-8°C.<br />
6. Pan G, Ni J, Yu G, Wei YF, Dixit VM. TRUNDD, a new member<br />
of the TRAIL receptor family that antagonizes TRAIL signalling.<br />
FEBS Lett 1998;424:41-5<br />
(RD1299)
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Anti-IKKα (C1)<br />
CATALOG No.: 2025<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli including IL-1, TNFα, and<br />
bacteria product LPS. NF-κB is associated with IκB proteins<br />
in the cell cytoplasm, which inhibit NF-κB activity. The longsought<br />
IκB kinase (IKK), which phosphorylates IκB, and<br />
mediates IκB degradation and NF-κB activation, was<br />
recently identified by several laboratories (1-5). IKK is a<br />
serine protein kinase, and the IKK complex contains alpha<br />
and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact<br />
with each other and both are essential for the NF-κB<br />
activation. IKKα specifically phosphorylates IκB-α. IKKα is<br />
expressed in variety of human tissues.<br />
SOURCE:<br />
Rabbit anti-IKKα (C1) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 716 to 734 of human<br />
IKKα (1,2), which differs from corresponding murine<br />
sequence by four amino acids.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IKKα<br />
by Western blot at 1:1000 to 1:2000 dilution. Whole cell<br />
lysate from HeLa or Jurkat cells can be used as positive<br />
control and an 85 kDa band should be detected. It has no<br />
cross response to IKKβ or IKKγ. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of IKKα<br />
in HeLa whole cell lysate with<br />
anti-IKKα (C1) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A<br />
cytokine-responsive IkappaB kinase that activates the transcription<br />
factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M.<br />
Identification and characterization of an IkappaB kinase. Cell<br />
1997;90:373-83<br />
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The<br />
IkappaB kinase complex (IKK) contains two kinase subunits,<br />
IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and<br />
NF-kappaB activation. Cell 1997;91:243-52<br />
4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB<br />
kinase-beta: NF-kappaB activation and complex formation with<br />
IkappaB kinase-alpha and NIK. Science 1997;278:866-9<br />
5. Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J,<br />
Young DB, Barbosa M, Mann M, Manning A, Rao A. IKK-1 and<br />
IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB<br />
activation. Science 1997; 278:860-6 (RD1299)
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Anti-ADAM10 (CT) KUZ, MADM<br />
CATALOG No.: 2051<br />
BACKGROUND:<br />
Proinflammatory cytokine tumor necrosis factor-α (TNF-α)<br />
contributes to a variety of inflammatory responses and<br />
programmed cell death. Notch receptor and its ligand<br />
participate in cell fate decisions during vertebrate<br />
development and are associated with several human<br />
disorders, including a T-cell lymphoma. TNF-α, notch and its<br />
ligand delta are all membrane-bond molecules, which are<br />
cleaved by proteases to release mature proteins or<br />
functional receptor. ADAM10, a metalloprotease-disintegrin<br />
in the family of mammalian ADAM (for a disintegrin and<br />
metalloprotease), was recently identified to cleave TNF-α,<br />
notch and its ligand delta (1-3). The genes encoding human,<br />
mouse, and bovine ADAM10 were recently cloned and<br />
designated ADAM 10, kuzbanian (KUZ), and MADM,<br />
respectively, (1,2,4). ADAM10 mRNA is expressed in a<br />
variety of human and bovine tissues (1,4).<br />
SOURCE:<br />
Rabbit anti-ADAM10 (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 732 to 748<br />
of human ADAM10 (1). This sequence is identical to those of<br />
bovine and rat origins and differs from that of mouse<br />
ADAM10 by one amino acid (2,4).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
ADAM10 by Western blot at 1:500 to 1:2000 dilution. Jurkat<br />
whole cell lysate can be used as positive control and an<br />
approximately 85 kDa band can be detected, which may<br />
represent precursor. A 60 kDa faint band was detected in<br />
some cell lines including Jurkat, which appears to be the<br />
processed mature protein. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at -20°C, stable for one year.<br />
REFERENCES:<br />
Western blot analysis of<br />
ADAM10 in Jurkat whole cell<br />
lysate in the absence (1) or<br />
presence (2) of blocking<br />
peptide (<strong>Catalog</strong> no. 2051P)<br />
with anti-ADAM10 (CT) at<br />
1:1000 dilution.<br />
1. Rosendahl MS, Ko SC, Long DL, et al. Identification and<br />
characterization of a pro-tumor necrosis factor-alpha-processing<br />
enzyme from the ADAM family of zinc metalloproteases. J Biol<br />
Chem 1997;272:24588-93<br />
2. Pan D, Rubin GM. Kuzbanian controls proteolytic processing of<br />
Notch and mediates lateral inhibition during Drosophila and<br />
vertebrate neurogenesis. Cell 1997;90:271-80<br />
3. Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T,<br />
Artavanis-Tsakonas S. Processing of the notch ligand delta by the<br />
metalloprotease Kuzbanian. Science 1999;283:91-4<br />
4. Howard L, Lu X, Mitchell S, Griffiths S, Glynn P. Molecular<br />
cloning of MADM: a catalytically active mammalian disintegrinmetalloprotease<br />
expressed in various cell types. Biochem J<br />
1996;317:45-50<br />
(RD1299)
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Anti-FLIP γ/δ (CT) FLIP, I-FLICE, FLAME-1, CASH, CLARP<br />
CATALOG No.: 2055<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain (DD)-containing<br />
adapter molecules and members of the ICE/CED-3 protease<br />
family. Caspases-8 (FLICE) and -10 (FLICE2) are two<br />
pivotal members in the ICE/CED-3 protease family. FLICEinhibitory<br />
proteins were identified in virus and human and<br />
designated v-FLIPs and c-FLIPs, respectively 1,2 . The human<br />
FLIPs were also cloned by several labs independently and<br />
termed Casper, I-FLICE, FLAME-1, CASH, CLARP and<br />
usurpin 3-7 . FLIP contains two death effector domains (DEDs)<br />
and a caspase-like domain. FLIP interacts with adapter<br />
protein FADD and caspase-8 and –10, and potently inhibits<br />
apoptosis induced by all known death receptors CD95, DR3,<br />
TRAIL-R and TNFR1. Four splice variants of c-FLIPs have<br />
been identified and termed FLIPα, β, γ, and δ, respectively 8 .<br />
SOURCE:<br />
Rabbit anti-FLIP (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 191 to 209 at C-<br />
terminus of human FLIPδ/FLIP s form 2 .<br />
APPLICATION:<br />
This antibody can be used for detection of FLIP by Western<br />
blot at 1:1000 dilution. Jurkat cell lysate can be used as<br />
positive control and 35 kDa and 25 kDa bands can be<br />
detected. This antibody recognizes human and murine FLIPγ<br />
and FLIPδ but not FLIPα and FLIPβ. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
Western blot analysis of<br />
FLIPγ/δ in total cell<br />
lysates from HeLa (H),<br />
Jurkat (J), THP-1 (T),<br />
A431 (A), K562 (K) and<br />
NIH3T3 (N) cells with<br />
anti-FLIPγ/δ (CT) at<br />
1:1000 dilution.<br />
REFERENCES:<br />
1. Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F,<br />
Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH,<br />
Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent<br />
apoptosis induced by death receptors . Nature 1997;386:517-521<br />
2. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V,<br />
Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE,<br />
Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature<br />
1997;388:190-195<br />
3. Shu HB, Halpin DR, Goeddel DV. Casper is a FADD- and caspase-related<br />
inducer of apoptosis. Immunity 1997;6:751-763<br />
4. Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of<br />
tumor necrosis factor receptor-1- and CD-95-induced apoptosis . J Biol Chem<br />
1997;272:17255-17257<br />
5. Srinivasula SM, Ahmad M, Ottilie S, Bullrich F, Banks S, Wang Y,<br />
Fernandes -Alnemri T, Croce CM, Litwack G, Tomaselli KJ, Armstrong RC,<br />
Alnemri ES. FLAME-1, a novel FADD-like anti-apoptotic molecule that<br />
regulates Fas/TNFR1-induced apoptosis. J Biol Chem 1997;272:18542-<br />
18545<br />
6. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM,<br />
Wallach D. CASH, a novel caspase homologue with death effector domains.<br />
J Biol Chem 1997;272:19641-19644<br />
7. Inohara N, Koseki T, Hu Y, Chen S, Nunez G. CLARP, a death effector<br />
domain-containing protein interacts with caspase-8 and regulates apoptosis.<br />
Proc Natl Acad Sci USA 1997;94:10717-10722<br />
8. Tschopp J, Irmler M, Thome M. Inhibition of fas death signals by FLIPs.<br />
Curr Opin Immunol 1998;10:552-8 (RD 1299)
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Anti-DNase II (CT)<br />
CATALOG No.: 2059<br />
BACKGROUND:<br />
Apoptosis is characterized by several morphological nuclear<br />
changes including chromatin condensation and nuclear<br />
fragmentation. These changes are triggered by the activation<br />
of members of caspase family, caspase activated DNase,<br />
and several novel proteins including AIF and Acinus (1).<br />
DNase II causes both chromatin condensation and DNA<br />
fragmentation (1). The genes encoding human (2-4), porcine<br />
(4), and murine (5) DNase II have been cloned. The DNase<br />
II gene encodes a 40 kDa proenzyme. The mature enzyme<br />
consists of two non-identical subunits, the 32 kDa (α) and 12<br />
kDa (β) chains, generated by proteolytic processing.<br />
Overexpression of DNase II induces chromatin condensation<br />
(3). DNase II is ubiquitously expressed in human tissues.<br />
SOURCE:<br />
Rabbit anti-DNase II (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 347 to 360<br />
of human DNase II precursor (2-4).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DNase<br />
II expression by Western blot at 1:500 to 1:1000 dilution.<br />
Human spleen tissue lysate or THP-1 cell lysate can be used<br />
as positive control and an approximate 40 kDa band can be<br />
detected, which represents the pro-enzyme of DNase II. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
DNase II in human spleen<br />
tissue lysate in the absence<br />
(lane 1) or presence (lane<br />
2) of blocking peptide with<br />
anti-DNase II (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature<br />
1999;401:127-8<br />
2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O,<br />
Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding<br />
human deoxyribonuclease II. J Biol Chem 1998;273:2610-6<br />
3. Krieser RJ, Eastman A. The cloning and expression of human<br />
deoxyribonuclease II. A possible role in apoptosis. J Biol Chem<br />
1998;273:30909-14<br />
4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine<br />
and human DNase II. Biochem Biophys Res Commun<br />
1998;247:864-9<br />
5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular<br />
cloning and characterization of human and murine DNase II. Gene<br />
1998;215:281-9<br />
(RD0400)
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Anti-Bim (IN) BOD<br />
CATALOG No.: 2065<br />
BACKGROUND:<br />
Members in the Bcl-2 family are critical regulators of<br />
apoptosis by either inhibiting or promoting cell death. Bcl-2<br />
homology 3 (BH3) domain is a potent death domain. BH3<br />
domain containing pro-apoptotic proteins, including Bad,<br />
Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2<br />
family. A novel BH3 domain containing protein was recently<br />
identified and designated Bim or BOD in human, mouse and<br />
rat (1,2). Bim/BOD interacts with diverse members in the<br />
pro-survival Bcl-2 sub-family including Bcl-2, Bcl-x L and Bclw.<br />
Bim/BOD induces apoptosis. The messenger RNA of Bim<br />
is ubiquitously expressed in multiple tissues and cell lines<br />
(1,2).<br />
SOURCE:<br />
Rabbit anti-Bim (IN) polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 22 to 40 of human<br />
origin (1). The sequence is identical to that of mouse and<br />
differs from that of rat by one amino acid.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Bim by<br />
Western blot at 1:1000 dilution. Human K562 cell lysate can<br />
be used as positive control and a 23 kDa band can be<br />
detected. This antibody is human, mouse, and rat reactive.<br />
This antibody is for research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of Bim in<br />
K562 (K) and A549 (A) whole cell<br />
lysates with anti-Bim (IN) at<br />
1:1000 dilution.<br />
REFERENCES:<br />
1. O'Connor,L., Strasser,A., O'Reilly,L.A., Hausmann,G.,<br />
Adams,J.M., Cory,S. and Huang,D.C. Bim: a novel member of the<br />
Bcl-2 family that promotes apoptosis. EMBO J. 1998;17:384-395<br />
2. Hsu, S.Y., Lin, P., and Hsueh, A.J. BOD (Bcl-2-related ovarian<br />
death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-<br />
2 protein capable of dimerization with diverse antiapoptotic Bcl-2<br />
members. Mol Endocrinol 1998;12:1432-40<br />
(RD1299)
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Anti-ZIP kinase (IN)<br />
CATALOG No.: 2067<br />
BACKGROUND:<br />
Apoptosis is mediated by death domain containing adapter<br />
molecules and a caspase family of proteases. Certain<br />
serine/threonine protein kinases, such as ASK-1 and RIP,<br />
are mediators of apoptosis. A novel serine/threonine kinase<br />
that mediates apoptosis was recently identified and<br />
designated ZIP kinase (1). ZIP kinase contains an N-terminal<br />
kinase domain and a C-terminal leucine zipper structure and<br />
binds to ATF4 that is a member of ATF/CREB family. ZIP<br />
kinase has high sequence homology to DAP kinase (deathassociated<br />
protein kinase), which is a mediator of apoptosis<br />
induced by gamma interferon. Overexpression of ZIP kinase<br />
induces apoptosis. ZIP and DAP kinases represent a novel<br />
kinase family, which mediates apoptosis through their<br />
catalytic activities. The messenger RNA was ubiquitously<br />
expressed in various tissues (1).<br />
STORAGE:<br />
Supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of ZIP<br />
kinase in HeLa (H) and Jurkat<br />
(J) whole cell lysates with<br />
anti-ZIP kinase (IN) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase,<br />
a novel serine/threonine kinase which mediates apoptosis. Mol Cell<br />
Biol 1998;18:1642-51<br />
(RD1299)<br />
SOURCE:<br />
Rabbit anti-ZIP kinase (IN) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 279 to 298<br />
of human origin (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of ZIP<br />
kinase by Western blot at 1:1000 dilution. Jurkat or HeLa<br />
whole cell lysate can be used as positive control and a 52<br />
kDa band should be detected. It has no cross response to<br />
DAP kinase. It is human, mouse, and rat reactive. For<br />
research use only.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Anti-Caspase-9 (I16) ICE-LAP6, Mch6, Apaf-3<br />
CATALOG No.: 2071<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
A novel member in the caspase family was recently<br />
identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3).<br />
Caspase-9 and Apaf-1 bind to each other, which leads to<br />
caspase-9 activation (3). Caspase-9 is also activated by<br />
granzyme B and CPP32 (1,2). Activated caspase-9 cleaves<br />
and activates caspase-3 that is one of the key proteases,<br />
being responsible for the proteolytic cleavage of many key<br />
proteins in apoptosis (3). Caspase-9 play a central role in<br />
cell death induced by a wide variety of apoptosis activators<br />
including TNFα, TRAIL, anti-CD-95, FADD, and TRADD (4).<br />
Caspase-9 is expressed in a variety of human tissues (1,2).<br />
SOURCE:<br />
Rabbit anti-caspase-9 (I16) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 41 to 56 of<br />
human caspase-9 (1-3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
caspase-9 by Western blot at 1:1000 to 1:2000 dilution.<br />
Whole cell lysate from HeLa cells can be used as positive<br />
control and a 46 kDa band should be detected. It has no<br />
cross response to other members in caspase family. It is<br />
human, mouse, and rat reactive. This antibody is for<br />
research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
caspase-9 in HeLa whole<br />
cell lysate with anticaspase-9<br />
(I16) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He<br />
WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene<br />
family, is activated by the cytotoxic T cell protease granzyme B. J<br />
Biol Chem 1996;271:16720-4<br />
2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N,<br />
Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G,<br />
Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like<br />
homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are<br />
substrates for the apoptotic mediator CPP32. J Biol Chem<br />
1996;271:27099-106<br />
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M,<br />
Alnemri ES, Wang X. Cytochrome c and dATP-dependent<br />
formation of Apaf-1/caspase-9 complex initiates an apoptotic<br />
protease cascade. Cell 1997;91:479-89<br />
4. Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1<br />
form a ternary complex. J Biol Chem 1998;273:5841-5<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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techsupport@prosci-inc.com<br />
Anti-Caspase-9 (I20) ICE-LAP6, Mch6, Apaf-3<br />
CATALOG No.: 2073<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
A novel member in the caspase family was recently<br />
identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3).<br />
Caspase-9 and Apaf-1 bind to each other, which leads to<br />
caspase-9 activation (3). Caspase-9 is also activated by<br />
granzyme B and CPP32 (1,2). Activated caspase-9 cleaves<br />
and activates caspase-3 that is one of the key proteases,<br />
being responsible for the proteolytic cleavage of many key<br />
proteins in apoptosis (3). Caspase-9 play a central role in<br />
cell death induced by a wide variety of apoptosis activators<br />
including TNFα, TRAIL, anti-CD-95, FADD, and TRADD (4).<br />
Caspase-9 is expressed in a variety of human tissues (1,2).<br />
SOURCE:<br />
Rabbit anti-caspase-9 (I20) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 299 to 318<br />
of human caspase-9 (1-3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
caspase-9 by Western blot at 1:1000 to 1:2000 dilution.<br />
Whole cell lysate from HeLa cells can be used as positive<br />
control and a 46 kDa band should be detected. It has no<br />
cross response to other members in caspase family. This<br />
antibody is for research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
caspase-9 in HeLa whole cell<br />
lysate with anti-caspase-9<br />
(I20) at 1:1000 dilution.<br />
REFERENCES:<br />
1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He<br />
WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene<br />
family, is activated by the cytotoxic T cell protease granzyme B. J<br />
Biol Chem 1996;271:16720-4<br />
2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N,<br />
Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G,<br />
Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like<br />
homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are<br />
substrates for the apoptotic mediator CPP32. J Biol Chem<br />
1996;271:27099-106<br />
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M,<br />
Alnemri ES, Wang X. Cytochrome c and dATP-dependent<br />
formation of Apaf-1/caspase-9 complex initiates an apoptotic<br />
protease cascade. Cell 1997;91:479-89<br />
4. Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1<br />
form a ternary complex. J Biol Chem 1998;273:5841-5.<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-RICK (NT) RIP2, CARDIAK<br />
CATALOG No.: 2075<br />
BACKGROUND:<br />
Apoptosis is mediated by death domain (DD) and/or caspase<br />
recruitment domain (CARD) containing molecules and a<br />
caspase family of proteases. DD-containing serine/threonine<br />
kinase RIP regulates Fas-induced apoptosis. A novel CARDcontaining<br />
serine/threonine kinase was recently identified<br />
and designated RICK/RIP2/CARDIAK for RIP-like interacting<br />
CLARP kinase, receptor interacting protein-2, and CARDcontaining<br />
ICE associated kinase, respectively, (1-3). RICK<br />
contains an N-terminal kinase catalytic domain and a C-<br />
terminal CARD domain. Overexpression of RICK induced<br />
apoptosis and activation of NF-κB and JNK. RICK interacts<br />
with members of the TRAF family, CLARP and caspase-1.<br />
Thus, RICK represents a novel kinase that regulates TNF<br />
and Fas induced-apoptosis and that is involved in the<br />
generation of proinflammatory cytokine IL-1β. The<br />
messenger RNA of RICK is expressed in multiple human<br />
tissues (1).<br />
SOURCE:<br />
Rabbit anti-RICK (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 11 to 30 of<br />
human origin (1).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
RICK in A431 (A) and K562<br />
(K) whole cell lysate with<br />
anti-RICK (NT) at 1:1000<br />
dilution.<br />
REFERENCES:<br />
1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a<br />
novel protein kinase containing a caspase recruitment domain,<br />
interacts with CLARP and regulates CD95-mediated apoptosis. J<br />
Biol Chem 1998;273:12296-300<br />
2. McCarthy JV, Ni J, Dixit VM. RIP2 is a novel NF-kappaBactivating<br />
and cell death-inducing kinase. J Biol Chem<br />
1998;273:16968-75<br />
3. Thome M, Hofmann K, Burns K, Martinon F, Bodmer JL,<br />
Mattmann C, Tschopp J. Identification of CARDIAK, a RIP-like<br />
kinase that associates with caspase-1. Curr Biol 1998;8:885-8<br />
(RD1299)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of RICK<br />
by Western blot at 1:1000 dilution. A431 or K562 whole cell<br />
lysate can be used as positive control and an approximately<br />
60 kDa band can be detected. It is human, mouse, and rat<br />
reactive. For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-ARC (CT)<br />
CATALOG No.: 2081<br />
BACKGROUND:<br />
Apoptosis is regulated by death domain (DD) and/or<br />
caspase recruitment domain (CARD) containing molecules<br />
and a caspase family of proteases. CARD domain containing<br />
cell death regulators include RAIDD, Apaf-1, caspase-9, and<br />
caspase-2. A novel CARD domain containing protein was<br />
recently identified and designated ARC for apoptosis<br />
repressor with CARD (1). ARC interacts with caspase-2 and<br />
–8 and inhibits enzymatic activity of caspase-8. ARC<br />
suppresses apoptosis induced by cell death adapters FADD<br />
and TRADD and by cell death receptors Fas, TNFR-1 and<br />
DR3. The messenger RNA of ARC is primarily expressed in<br />
skeletal muscle and cardiac tissue (1).<br />
SOURCE:<br />
Rabbit anti-ARC (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 191 to 208 of human<br />
origin (1).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
ARC in HeLa (H), KB (K),<br />
and A549 (A) whole cell<br />
lysates with anti-ARC (CT)<br />
at 1:1000 dilution.<br />
REFERENCES:<br />
1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of<br />
apoptosis expressed in skeletal muscle and heart that interacts<br />
selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-<br />
60 (RD1299)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of ARC<br />
by Western blot at 1:1000 dilution. Whole cell lysate from<br />
HeLa cells can be used as positive control and an<br />
approximately 25 kDa band can be detected. This antibody<br />
is for research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-CIDE-A (CT)<br />
CATALOG No.: 2085<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors. Cell death signals are<br />
transduced by DD-, DED-, or CARD-containing molecules<br />
and members of the caspase family of proteases. These<br />
death signals finally cause the degradation of chromosomal<br />
DNA by activated DNase DFF40/CAD, which is chaperoned<br />
and inhibited by DFF45/ICAD (1-4). DFF45 related proteins<br />
CIDE-A and CIDE-B (for cell death-inducing DFF-like<br />
effector A and B) were recently identified (5). CIDE contains<br />
a new type of domain termed CIDE-N, which has high<br />
homology with the regulatory domains of DFF45/ICAD and<br />
DFF40/CAD (5,6). Expression of CIDE-A induces DNA<br />
fragmentation and activates apoptosis, which is inhibited by<br />
DFF45. CIDE-A is expressed in many tissues.<br />
SOURCE:<br />
Rabbit anti-CIDE-A (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 200 to 217<br />
of human CIDE-A (5).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of CIDE-A<br />
by Western blot at 1:1000 to 1:2000 dilution. Human brain<br />
tissue lysate can be used as positive control and an<br />
approximately 23 kDa band can be detected. It has no cross<br />
activity to CIDE-B. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2085P). Human brain tissue lysate,<br />
200 µl/100 µl, is available for positive control (<strong>Catalog</strong> No.<br />
1303).<br />
Western blot analysis of<br />
CIDE-A in human brain tissue<br />
lysate in the absence (A) or<br />
presence (B) of peptide<br />
(2085P) with anti-CIDE-A<br />
(CT) at 1:2000 dilution.<br />
REFERENCES:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184 2. Enari M,<br />
Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A<br />
caspase-activated DNase that degrades DNA during apoptosis,<br />
and its inhibitor ICAD. Nature 1998;391:43-50<br />
3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
5. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel<br />
family of cell death activators with homology to the 45 kDa subunit<br />
of the DNA fragmentation factor. EMBO J 1998;17:2526-33<br />
6. Lugovskoy AA, Zhou P, Chou JJ, McCarty JS, Li P, Wagner G.<br />
Solution structure of the CIDE-N domain of CIDE -B and a model<br />
for CIDE-N/CIDE -N interactions in the DNA fragmentation pathway<br />
of apoptosis. Cell 1999;99(7):747-55<br />
(WD0300)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-CIDE-A (CT)<br />
CATALOG No.: 2089<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. DFF45/ICAD has<br />
been identified as inhibitor of caspase activated DNase<br />
DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B<br />
(for cell death-inducing DFF-like effector A and B) were<br />
recently identified (1). CIDE contains a new type of domain<br />
termed CIDE-N, which has high homology with the<br />
regulatory domains of DFF45/ICAD and DFF40/CAD (1,2).<br />
Expression of CIDE-A induces DNA fragmentation and<br />
activates apoptosis, which is inhibited by DFF45. CIDE-A is<br />
a DFF45-inhibitable effector that promotes cell death and<br />
DNA fragmentation. CIDE-A is expressed in many tissues.<br />
STORAGE:<br />
Supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
CIDE-A in murine heart<br />
tissue lysate with anti-CIDE-A<br />
(CT) at 1:500 dilution.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 100 µg / 500 µl, is available for<br />
competition studies (<strong>Catalog</strong> No. 2089P).<br />
Murine heart tissue lysate, 500 µg / 200 µl, is available for<br />
positive control (<strong>Catalog</strong> No. 1253).<br />
SOURCE:<br />
Rabbit anti-CIDE-A (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 200 to 214<br />
of mouse CIDE-A (1).<br />
REFERENCES:<br />
1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel<br />
family of cell death activators with homology to the 45 kDa subunit<br />
of the DNA fragmentation factor. EMBO J 1998;17:2526-33<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of CIDE-A<br />
by Western blot at 1:500 to 1:1000 dilution. Tissue lysate of<br />
murine heart can be used as positive control and an<br />
approximately 25 kDa band can be detected. It has no cross<br />
activity to CIDE-B. This antibody is for research use only.<br />
2. Inohara N, Koseki T, Chen S, Benedict MA, Nunez G.<br />
Identification of regulatory and catalytic domains in the apoptosis<br />
nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-CIDE-B (CT)<br />
CATALOG No.: 2091<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. DFF45/ICAD has<br />
been identified as inhibitor of caspase activated DNase<br />
DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B<br />
(for cell death-inducing DFF-like effector A and B) were<br />
recently identified (1). CIDE contains a new type of domain<br />
termed CIDE-N, which has high homology with the<br />
regulatory domains of DFF45/ICAD and DFF40/CAD (1,2).<br />
Expression of CIDE-B induces apoptosis, which is inhibited<br />
by DFF45. CIDE-B is a DFF45-inhibitable effector that<br />
promotes cell death and DNA fragmentation. CIDE-B is<br />
expressed mainly in liver and at lower levels in spleen,<br />
kidney, peripheral blood lymphocytes, and bone marrow (1).<br />
SOURCE:<br />
Rabbit anti-CIDE-B (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 204 to 219<br />
of murine CIDE-B (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of CIDE-B<br />
by Western blot at 1:500 to 1:1000 dilution. Tissue lysate of<br />
murine liver can be used as positive control and an<br />
approximately 25 kDa band can be detected. It has no cross<br />
activity to CIDE-A. This antibody is for research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
CIDE-B in murine liver tissue<br />
lysate with anti-CIDE-B (CT)<br />
at 1:500 dilution.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 100 µg / 500 µl, is available for<br />
competition studies (<strong>Catalog</strong> No. 2091P).<br />
Murine liver tissue lysate, 500 µg / 200 µl, is available for<br />
positive control (<strong>Catalog</strong> No. 1254).<br />
REFERENCES:<br />
1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel<br />
family of cell death activators with homology to the 45 kDa subunit<br />
of the DNA fragmentation factor. EMBO J 1998;17:2526-33<br />
2. Inohara N, Koseki T, Chen S, Benedict MA, Nunez G.<br />
Identification of regulatory and catalytic domains in the apoptosis<br />
nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-CX 3 CR1 (NT) V28, CMKBRL1<br />
CATALOG NO.: 2093<br />
BACKGROUND:<br />
CX 3 CR1 is one of the chemokine receptors that are required<br />
as coreceptors for HIV infection. The genes encoding<br />
human, murine, and rat CX 3 CR1 were cloned and<br />
designated V28 and CMKBRL1, CX 3 CR1, and RBS11,<br />
respectively, (1-4). The encoded seven transmembrane<br />
protein was recently identified as the receptor for a novel<br />
transmembrane molecule, fractalkine, and renamed CX 3 CR1<br />
(5). Recently, CX 3 CR1 was found to serve as a coreceptor<br />
for HIV-1 and HIV-2 envelope fusion and virus infection,<br />
which can be inhibited by fractokine (6). CX 3 CR1 mediates<br />
leukocyte migration and adhesion (5). CX 3 CR1 is expressed<br />
in a variety of human tissues and cell lines (2).<br />
SOURCE:<br />
Rabbit anti-CX 3 CR1 polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 2 to 21 of human<br />
CX 3 CR1 (1,2). The sequence differs from those of mouse<br />
and rat CX 3 CR1 by four amino acids (3,4)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
CX 3 CR1 by Western blot at 1:500 to 1:2000 dilution. Human<br />
spleen tissue lysate can be used as positive control and an<br />
approximately 50 kDa band can be detected. It is human,<br />
mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
CX 3 CR1 in human spleen<br />
lysate with anti-CX 3 CR1 in the<br />
absence (lane 1) or presence<br />
of blocking peptide (lane 2).<br />
REFERENCES:<br />
1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The<br />
orphan G-protein-coupled receptor-encoding gene V28 is closely related to<br />
genes for chemokine receptors and is expressed in lymphoid and neural<br />
tissues. Gene 1995;163:295-9<br />
2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization,<br />
and RNA expression of a human beta chemokine receptor-like gene. DNA<br />
Cell Biol 1995;14:673-80<br />
3. Combadiere C, Gao J, Tiffany HL, Murphy PM. Gene cloning, RNA<br />
distribution, and functional expression of mCX3CR1, a mouse chemotactic<br />
receptor for the CX3C chemokine fractalkine. Biochem Biophys Res<br />
Commun 1998;253:728-32<br />
4. Harrison JK, Barber CM, Lynch KR. cDNA cloning of a G-protein-coupled<br />
receptor expressed in rat spinal cord and brain related to chemokine<br />
receptors. Neurosci Lett 1994;169:85-9<br />
5. Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki<br />
M, Takagi S, Nomiyama H, Schall TJ, Yoshie O. Identification and molecular<br />
characterization of fractalkine receptor CX3CR1, which mediates both<br />
leukocyte migration and adhesion. Cell 1997;91:521-30<br />
6. Combadiere C, Salzwedel K, Smith ED, Tiffany HL, Berger EA, Murphy<br />
PM. Identification of CX3CR1. A chemotactic receptor for the human CX3C<br />
chemokine fractalkine and a fusion coreceptor for HIV-1. J Biol Chem<br />
1998;273:23799-804 (RD1299)
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Anti-CCR8 TER1, CKR-L1, ChemR1<br />
CATALOG NO.: 2097<br />
BACKGROUND:<br />
CCR8 is one of the chemokine receptors that are required as<br />
coreceptors for HIV infection. The genes encoding human<br />
and murine CCR8 were cloned and designated TER1, CKR-<br />
L1, and ChemR1 (1-4). The encoded seven transmembrane<br />
protein was identified as the receptor for human CC<br />
chemokine I-309 and renamed CCR8. Recently, CCR8 was<br />
found to serve as a coreceptor for diverse T-cell tropic, dualtropic<br />
and macrophage-tropic HIV-1 strains (5). CCR8<br />
mediates CC chemokine I-309 induced monocyte<br />
chemoattraction and HIV-1 envelope fusion and virus<br />
infection, which can be prevented by the CCR8 ligand I-309.<br />
CCR8 is expressed in spleen, thymus and T lymphoblastic<br />
cell lines.<br />
SOURCE:<br />
Rabbit anti-CCR8 polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 183 to 201 of human<br />
CCR8, which locate in the second extracellular loop (1-3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of CCR8<br />
by Western blot at 1:500 to 1:1000 dilution. Human spleen<br />
tissue lysate can be used as positive control and an<br />
approximately 50 kDa band can be detected. For research<br />
use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
CCR8 in human spleen lysate<br />
with anti-CCR8 at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A,<br />
Storlazzi CT, Rocchi M, Santoni A. Molecular cloning of TER1, a<br />
chemokine receptor-like gene expressed by lymphoid tissues. J<br />
Immunol 1996;157:2759-63<br />
2. Zaballos A, Varona R, Gutierrez J, Lind P, Marquez G.<br />
Molecular cloning and RNA expression of two new human<br />
chemokine receptor-like genes. Biochem Biophys Res Commun<br />
1996;227:846-53<br />
3. Samson M, Stordeur P, Labbe O, Soularue P, Vassart G,<br />
Parmentier M. Molecular cloning and chromosomal mapping of a<br />
novel human gene, ChemR1, expressed in T lymphocytes and<br />
polymorphonuclear cells and encoding a putative chemokine<br />
receptor. Eur J Immunol 1996;26:3021-8<br />
4. Goya I, Gutierrez J, Varona R, Kremer L, Zaballos A, Marquez<br />
G. Identification of CCR8 as the specific receptor for the human<br />
beta-chemokine I-309: cloning and molecular characterization of<br />
murine CCR8 as the receptor for TCA-3. J Immunol<br />
1998;160:1975-81<br />
5. Horuk R, Hesselgesser J, Zhou Y, Faulds D, Halks-Miller M,<br />
Harvey S, Taub D, Samson M, Parmentier M, Rucker J, Doranz<br />
BJ, Doms RW. The CC chemokine I-309 inhibits CCR8-dependent<br />
infection by diverse HIV-1 strains. J Biol Chem 1998;273:386-91<br />
(WD1299)
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Anti-GPR15 (NT) BOB<br />
CATALOG NO.: 2101<br />
BACKGROUND:<br />
Human immunodeficiency virus (HIV) and related viruses<br />
require coreceptors to infect target cells. Some G proteincoupled<br />
receptors including CCR5, CXCR4, CCR3, CCR2b<br />
and CCR8 in the chemokine receptor family, and four new<br />
human molecules GPR15, STRL33, GPR1 and V28 were<br />
recently identified as HIV coreceptors. The genes encoding<br />
human and monkey GPR15/BOB (for G protein-coupled<br />
receptor 15 and brother of Bonzo, respectively,) were<br />
recently cloned (1,2). This novel G protein-coupled receptor<br />
serves as corecptor for simian immunodeficiency virus (SIV),<br />
and for strains of HIV-2 and M-tropic HIV-1 (2,3). The ligand<br />
for GPR15 has not been identified yet.<br />
SOURCE:<br />
Rabbit anti-GPR15/BOB polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 13 to 28 of<br />
human GPR15 (1). The sequence differs from those of<br />
African green monkey and pig-tailed macaque BOB by one<br />
amino acid (2)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
GPR15/BOB by Western blot at 1:500 to 1:2000 dilution.<br />
Jurkat or A549 cell lysate can be used as positive control<br />
and an approximately 50 kDa band can be detected. It is<br />
human, mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of GPR15 in<br />
human heart lysate with anti-<br />
GPR15 (NT) in the absence (lane<br />
1) or presence of specific peptide<br />
(lane 2) or non-related peptide<br />
(lane 3).<br />
REFERENCES:<br />
1. Heiber M, Marchese A, Nguyen T, Heng HH, George SR,<br />
O'Dowd BF. A novel human gene encoding a G-proteincoupled<br />
receptor (GPR15) is located on chromosome 3. Genomics<br />
1996;32:462-5<br />
2. Deng HK, Unutmaz D, KewalRamani VN, Littman DR.<br />
Expression cloning of new receptors used by simian and human<br />
immunodeficiency viruses. Nature 199;388:296-300<br />
3. Farzan M, Choe H, Martin K, Marcon L, Hofmann W, Karlsson<br />
G, Sun Y, Barrett P, Marchand N, Sullivan N, Gerard N, Gerard C,<br />
Sodroski J. Two orphan seven-transmembrane segment receptors<br />
which are expressed in CD4-positive cells support simian<br />
immunodeficiency virus infection. J Exp Med 1997;186:405-11<br />
(RD1299)
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Anti-DFF40/CAD (I18)<br />
CATALOG No.: 2107<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A mouse DNase<br />
that causes DNA fragmentation was identified recently and<br />
designated CAD (for caspase activated deoxyribonuclease)<br />
(1,2). The human homologue of mouse CAD was more<br />
recently identified by three groups independently and termed<br />
CPAN, DFF40, and human CAD, respectively, (3-5).<br />
DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6)<br />
and forms complex with DFF40/CAD. Upon cleavage of<br />
DFF45/ICAD by activated caspase, DFF40/CAD is released<br />
and activated and eventually causes the degradation of DNA<br />
in the nuclei. Activation of DFF40/CAD, which causes DNA<br />
degradation, is the hallmark of apoptotic cell death.<br />
SOURCE:<br />
Rabbit anti-CAD (I18) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 147 to 164 of murine<br />
CAD (1). The sequence differs from human DFF40 by two<br />
amino acids (3,4)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
DFF40/CAD by Western blot at 1:500 to 1:1000 dilution.<br />
K562 or Jurkat whole cell lysate can be used as positive<br />
control and a 40 kDa band can be detected. It is human,<br />
mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100 µg<br />
in 200 µl of PBS containing 0.02% sodium azide. Store at<br />
4°C, stable for one year.<br />
Western blot analysis of<br />
DFF40/CAD in HeLa (H),<br />
K562 (K), Jurkat (J), and<br />
Raji (R) whole cell lysate<br />
with anti-DFF40/CAD (I18)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
3. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L,<br />
Williams LT. CPAN, a human nuclease regulated by the caspasesensitive<br />
inhibitor DFF45. Curr Biol 1998;8:537-40<br />
4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H,<br />
Nagata S. Molecular cloning + characterization of human caspaseactivated<br />
DNase. Proc Natl Acad Sci USA 1998;95:9123-8<br />
6. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
(RD1299)
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Anti-IKKα/IKK-1 (C2)<br />
CATALOG No.: 2115<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli including IL-1, TNFα and<br />
bacteria product LPS. NF-κB is associated with IκB proteins<br />
in the cell cytoplasm, which inhibit NF-κB activity. The longsought<br />
IκB kinase (IKK), which phosphorylates IκB, and<br />
mediates IκB degradation and NF-κB activation, was<br />
recently identified by several laboratories (1-5). IKK is a<br />
serine protein kinase, and the IKK complex contains alpha<br />
and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact<br />
with each other and both are essential for the NF-κB<br />
activation. IKKα specifically phosphorylates IκB-α. IKKα is<br />
expressed in a variety of human tissues.<br />
SOURCE:<br />
Rabbit anti-IKKα (C2) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 699 to 715 of human<br />
IKKα (1,2), which differs from corresponding murine<br />
sequence by one amino acid.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IKKα<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from HeLa cells can be used as positive control and an 85<br />
kDa band should be detected. It has no cross response to<br />
IKKβ or IKKγ. For research use only.<br />
Western blot analysis of<br />
IKKα in HeLa whole cell<br />
lysate with anti-IKKα (C2)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A<br />
cytokine-responsive IkappaB kinase that activates the transcription<br />
factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M.<br />
Identification and characterization of an IkappaB kinase. Cell<br />
1997;90:373-83<br />
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The<br />
IkappaB kinase complex (IKK) contains two kinase subunits,<br />
IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and<br />
NF-kappaB activation. Cell 1997;91:243-52<br />
4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB<br />
kinase-beta: NF-kappaB activation and complex formation with<br />
IkappaB kinase-alpha and NIK. Science 1997;278:866-9<br />
5. Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J,<br />
Young DB, Barbosa M, Mann M, Manning A, Rao A. IKK-1 and<br />
IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB<br />
activation. Science 1997;278:860-6 (RD1299)<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.
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techsupport@prosci-inc.com<br />
Anti-IKKα (C3)<br />
CATALOG No.: 2117<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli including IL-1, TNFα, and<br />
bacteria product LPS. NF-κB is associated with IκB proteins<br />
in the cell cytoplasm, which inhibit NF-κB activity. The longsought<br />
IκB kinase (IKK), which phosphorylates IκB, and<br />
mediates IκB degradation and NF-κB activation, was<br />
recently identified by several laboratories (1-5). IKK is a<br />
serine protein kinase, and the IKK complex contains alpha<br />
and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact<br />
with each other and both are essential for the NF-κB<br />
activation. IKKα specifically phosphorylates IκB-α. IKKα is<br />
expressed in a variety of human tissues.<br />
SOURCE:<br />
Rabbit anti-IKKα (C3) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 658 to 674 of human<br />
IKKα (1,2), which differs from corresponding murine<br />
sequence by one amino acid.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IKKα<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from Jurkat cells can be used as positive control and an 85<br />
kDa band should be detected. It has no cross response to<br />
IKKβ or IKKγ. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
IKKα in Jurkat whole cell<br />
lysate with anti-IKKα (C3)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A<br />
cytokine-responsive IkappaB kinase that activates the transcription<br />
factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M.<br />
Identification and characterization of an IkappaB kinase. Cell<br />
1997;90:373-83<br />
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The<br />
IkappaB kinase complex (IKK) contains two kinase subunits,<br />
IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and<br />
NF-kappaB activation. Cell 1997;91:243-52<br />
4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB<br />
kinase-beta: NF-kappaB activation and complex formation with<br />
IkappaB kinase-alpha and NIK. Science 1997;278:866-9<br />
5. Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J,<br />
Young DB, Barbosa M, Mann M, Manning A, Rao A. IKK-1 and<br />
IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB<br />
activation. Science 1997;278:860-6<br />
(RD1299)
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techsupport@prosci-inc.com<br />
Anti-IKKβ (C3)<br />
CATALOG No.: 2121<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli including IL-1, TNFα, and<br />
bacteria product LPS. NF-κB is associated with IκB proteins<br />
in the cell cytoplasm, which inhibit NF-κB activity. The longsought<br />
IκB kinase (IKK), which phosphorylates IκB, and<br />
mediates IκB degradation and NF-κB activation, was<br />
recently identified by several laboratories (1-5). IKK is a<br />
serine protein kinase, and the IKK complex contains alpha<br />
and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact<br />
with each other and both are essential for NF-κB activation.<br />
IKKβ phosphorylates both IκB-α and IκB-β. IKKβ is<br />
expressed in variety of human tissues.<br />
SOURCE:<br />
Rabbit anti-IKKβ (C3) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 662 to 680 of human<br />
IKKβ (3,4), which differs from corresponding murine<br />
sequence by one amino acid (5).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IKKβ<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from Jurkat cells can be used as positive control and an 87<br />
kDa band should be detected. It has no cross response to<br />
IKKα or IKKγ. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of IKKβ<br />
in Jurkat whole cell lysate with<br />
anti-IKKβ (C3) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A<br />
cytokine-responsive IkappaB kinase that activates the transcription<br />
factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M.<br />
Identification and characterization of an IkappaB kinase. Cell<br />
1997;90:373-83<br />
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The<br />
IkappaB kinase complex (IKK) contains two kinase subunits,<br />
IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and<br />
NF-kappaB activation. Cell 1997;91:243-52<br />
4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB<br />
kinase-beta: NF-kappaB activation and complex formation with<br />
IkappaB kinase-alpha and NIK. Science 1997;278:866-9<br />
5. Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J,<br />
Young DB, Barbosa M, Mann M, Manning A, Rao A. IKK-1 and<br />
IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB<br />
activation. Science 1997;278:860-6<br />
6. Nakano H, Shindo M, Sakon S, Nishinaka S, Mihara M, Yagita<br />
H, Okumura K. Differential regulation of IkappaB kinase alpha and<br />
beta by two upstream kinases, NF-kappaB-inducing kinase and<br />
mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl<br />
Acad Sci U S A 1998;95:3537-42<br />
(RD1299)
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Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
Anti-IRAK2 (C2)<br />
CATALOG No.: 2123<br />
BACKGROUND:<br />
The pro-inflammatory cytokine IL-1 induces cellular<br />
response through two subunits of its receptor, IL-1 receptor I<br />
(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1<br />
receptor-associated kinase (IRAK) mediates activation of<br />
NF-κB, which is a pivotal transcription factor mediating<br />
inflammatory and immune response. A novel member in the<br />
IRAK/Pelle family was recently identified and designated<br />
IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of<br />
the IL-1R complex after IL-1 binding and lead to NF-κB<br />
activation. IRAKs also associate with Toll like receptor (TLR)<br />
and the dominant negative mutants of IRAKs inhibit LPSinduced<br />
NF-κB activation (2,3). Members in IRAK/Pelle<br />
family play a central role in IL-1R and TLR mediated<br />
inflammatory response. IRAK2 is expressed in a variety of<br />
human tissues.<br />
SOURCE:<br />
Rabbit anti-IRAK2 (C2) polyclonal antibody was raised<br />
against a peptide corresponding to amino acid 546 to 564 of<br />
human IRAK2 (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IRAK2<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from HeLa or K562 cells can be used as positive control and<br />
a 65 kDa band should be detected. Anti-IRAK2 has no cross<br />
response to IRAK. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
IRAK2 in HeLa (H) and K562<br />
(K) whole cell lysate with anti-<br />
IRAK2 (C2) at 1:500 dilution.<br />
REFERENCES:<br />
1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member<br />
IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.<br />
Science 1997;278:1612-5<br />
2. Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E,<br />
Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial<br />
lipopolysaccharide activates nuclear factor-kappaB through<br />
interleukin-1 signaling mediators in cultured human dermal<br />
endothelial cells and mononuclear phagocytes. J Biol Chem<br />
1999;274:7611-4<br />
3. Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events<br />
induced by lipopolysaccharide-activated toll-like receptor 2. J<br />
Immunol 1999;163:639-43<br />
(RD1299)
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techsupport@prosci-inc.com<br />
Anti-MyD88 (IN)<br />
CATALOG No.: 2125<br />
BACKGROUND:<br />
The pro-inflammatory cytokine IL-1 induced cellular<br />
response requires IL-1 receptor complex including IL-1RI<br />
and IL-1RAcP. Recently, MyD88 was identified as an<br />
adapter molecule in the IL-1 signaling pathway (1). MyD88<br />
associates with and recruits IRAK to the IL-1 receptor<br />
complex in response to IL-1 treatment and dominant<br />
negative form of MyD88 attenuates IL-1R-mediated NF-κB<br />
activation. MyD88 is also employed as a regulator molecule<br />
by IL-18 receptor and human Toll receptor (2,3), which are<br />
members in the Toll/IL-1R family of receptors. Targeted<br />
disruption of the MyD88 gene results in lose of cellular<br />
responses to IL-1 and IL-18, and MyD88-deficient mice lack<br />
responses to bacterial product LPS that employs Toll-like<br />
receptors 2 and 4 (TLR2 and TLR4) as the signaling<br />
receptors. MyD88 is a general adapter protein for the<br />
Toll/IL-1R family of receptors and plays an important role in<br />
the inflammatory response induced by cytokines IL-1 and IL-<br />
18 and endotoxin. MyD88 gene is expressed in many<br />
tissues.<br />
SOURCE:<br />
Rabbit anti-MyD88 (IN) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 233 to 248<br />
of human MyD88 (5), which differ from those of mouse by<br />
two amino acids.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of MyD88<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from Jurkat cells can be used as positive control and an<br />
approximately 35 kDa band can be detected. It is human and<br />
mouse reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
MyD88 in Jurkat whole cell<br />
lysate with anti-MyD88 (IN)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member<br />
IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.<br />
Science 1997;278:1612-5<br />
2. Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H,<br />
Sakagami M, Nakanishi K, Akira S. Targeted disruption of the<br />
MyD88 gene results in loss of IL-1- and IL-18-mediated function.<br />
Immunity 1998;9:143-50<br />
3. Medzhitov R, Preston-Hurlburt P, Kopp E, Stadlen A, Chen C,<br />
Ghosh S, Janeway CA Jr. MyD88 is an adaptor protein in the<br />
hToll/IL-1 receptor family signaling pathways. Mol Cell 1998;2:253-<br />
8<br />
4. Kawai T, Adachi O, Ogawa T, Takeda K, Akira S.<br />
Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity<br />
1999;11:115-22<br />
5. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE.<br />
The cloning and characterization of human MyD88: amember of an<br />
IL-1 receptor related family. FEBS Lett 1997;402:81-4<br />
(RD1299)
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Anti-MyD88 (CT)<br />
CATALOG No.: 2127<br />
BACKGROUND:<br />
The pro-inflammatory cytokine IL-1 induced cellular<br />
response requires IL-1 receptor complex including IL-1RI<br />
and IL-1RAcP. Recently, MyD88 was identified as an<br />
adapter molecule in the IL-1 signaling pathway (1). MyD88<br />
associates with and recruits IRAK to the IL-1 receptor<br />
complex in response to IL-1 treatment and dominant<br />
negative form of MyD88 attenuates IL-1R-mediated NF-κB<br />
activation. MyD88 is also employed as a regulator molecule<br />
by IL-18 receptor and human Toll receptor (2,3), which are<br />
members in the Toll/IL-1R family of receptors. Targeted<br />
disruption of the MyD88 gene results in lose of cellular<br />
responses to IL-1 and IL-18, and MyD88-deficient mice lack<br />
responses to bacterial product LPS that employs Toll-like<br />
receptors 2 and 4 (TLR2 and TLR4) as the signaling<br />
receptors. MyD88 is a general adapter protein for the<br />
Toll/IL-1R family of receptors and plays an important role in<br />
the inflammatory response induced by cytokines IL-1 and IL-<br />
18 and endotoxin. MyD88 gene is expressed in many<br />
tissues.<br />
SOURCE:<br />
Rabbit anti-MyD88 (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 279 to 296<br />
of human MyD88 (5), which are identical to those of mouse.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of MyD88<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from Jurkat cells can be used as positive control and an<br />
approximately 35 kDa band can be detected. It is human and<br />
mouse reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
MyD88 in Jurkat whole cell<br />
lysate with anti-MyD88 (CT)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member<br />
IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.<br />
Science 1997;278:1612-5<br />
2. Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H,<br />
Sakagami M, Nakanishi K, Akira S. Targeted disruption of the<br />
MyD88 gene results in loss of IL-1- and IL-18-mediated function.<br />
Immunity 1998;9:143-50<br />
3. Medzhitov R, Preston-Hurlburt P, Kopp E, Stadlen A, Chen C,<br />
Ghosh S, Janeway CA Jr. MyD88 is an adaptor protein in the<br />
hToll/IL-1 receptor family signaling pathways. Mol Cell 1998;2:253-<br />
8<br />
4. Kawai T, Adachi O, Ogawa T, Takeda K, Akira S.<br />
Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity<br />
1999;11:115-22<br />
5. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE.<br />
The cloning and characterization of human MyD88: a member of<br />
an IL-1 receptor related family. FEBS Lett 1997;402:81-4<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
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Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
Anti-IL-1RAcP (C2)<br />
CATALOG No.: 2129<br />
BACKGROUND:<br />
The pro-inflammatory cytokine IL-1 induced cellular<br />
response requires two subunits of its receptor, IL-1 receptor I<br />
(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1).<br />
IL-1RAcP forms a complex with IL-1RI in response to IL-1<br />
treatment. The IL-1 receptor-associated kinase (IRAK),<br />
which mediates activation of NF-κB inducing kinae (NIK) and<br />
of NF-κB, recruits to the IL-1R complex through IL-1RAcP<br />
(2). IL-1 activation of stress-activated protein kinase and of<br />
acid sphingomyelinase also requires IL-1RAcP (3,4). Like<br />
IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated<br />
cellular response. IL-1RAcP is expressed in many tissues.<br />
SOURCE:<br />
Rabbit anti-IL-1RAcP (C2) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 525 to 540<br />
of human IL-1RAcP, which is identical to those of mouse and<br />
rat origins (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IL-<br />
1RAcP by Western blot at 1:1000 to 1:2000 dilution. Whole<br />
cell lysate from HeLa cells can be used as positive control<br />
and an approximately 66 kDa band can be detected. It is<br />
human, mouse, and rat reactive, and has no cross activity to<br />
other members in the IL-1 receptor family. For research use<br />
only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of IL-<br />
1RAcP in HeLa whole cell<br />
lysate with anti-IL-1RAcP (C2)<br />
at 1:1000 dilution.<br />
REFERENCES:<br />
1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju<br />
G. Molecular cloning and characterization of a second subunit of<br />
the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-<br />
65<br />
2. Huang J, Gao X, Li S, Cao Z. Recruitment of IRAK to the<br />
interleukin 1 receptor complex requires interleukin 1 receptor<br />
accessory protein. Proc Natl Acad Sci U S A 1997;94:12829-32<br />
3. Wesche H, Korherr C, Kracht M, Falk W, Resch K, Martin MU.<br />
The interleukin-1 receptor accessory protein (IL-1RAcP) is<br />
essential for IL-1-induced activation of interleukin-1 receptorassociated<br />
kinase (IRAK) and stress-activated protein kinases<br />
(SAP kinases). J Biol Chem 1997;272:7727-31<br />
4. Hofmeister R, Wiegmann K, Korherr C, Bernardo K, Kronke M,<br />
Falk W. Activation of acid sphingomyelinase by interleukin-1 (IL-1)<br />
requires the IL-1 receptor accessory protein. J Biol Chem<br />
1997;272:27730-6<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IL-1RAcP (CT)<br />
CATALOG No.: 2131<br />
BACKGROUND:<br />
The pro-inflammatory cytokine IL-1 induced cellular<br />
response requires two subunits of its receptor, IL-1 receptor I<br />
(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1).<br />
IL-1RAcP forms a complex with IL-1RI in response to IL-1<br />
treatment. The IL-1 receptor-associated kinase (IRAK),<br />
which mediates activation of NF-κB inducing kinae (NIK) and<br />
of NF-κB, recruits to the IL-1R complex through IL-1RAcP<br />
(2). IL-1 activation of stress-activated protein kinase and of<br />
acid sphingomyelinase also requires IL-1RAcP (3,4). Like<br />
IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated<br />
cellular response. IL-1RAcP is expressed in many tissues.<br />
SOURCE:<br />
Rabbit anti-IL-1RAcP (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 549 to 569<br />
of human IL-1RAcP, which differs from the sequence of<br />
mouse origin by three amino acids (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IL-<br />
1RAcP by Western blot at 1:500 to 1:1000 dilution. Whole<br />
lysate from HeLa cells can be used as positive control and<br />
an approximately 66 kDa band can be detected. It has no<br />
cross activity to other members in the IL-1 receptor family.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of IL-<br />
1RAcP in HeLa whole cell<br />
lysate with anti-IL-1RAcP<br />
(CT) at 1:500 dilution.<br />
REFERENCES:<br />
1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju<br />
G. Molecular cloning and characterization of a second subunit of<br />
the interleukin 1 receptor complex. J Biol Chem 1995;270:13757-<br />
65<br />
2. Huang J, Gao X, Li S, Cao Z. Recruitment of IRAK to the<br />
interleukin 1 receptor complex requires interleukin 1 receptor<br />
accessory protein. Proc Natl Acad Sci U S A 1997;94:12829-32<br />
3. Wesche H, Korherr C, Kracht M, Falk W, Resch K, Martin MU.<br />
The interleukin-1 receptor accessory protein (IL-1RAcP) is<br />
essential for IL-1-induced activation of interleukin-1 receptorassociated<br />
kinase (IRAK) and stress-activated protein kinases<br />
(SAP kinases). J Biol Chem 1997;272:7727-31<br />
4. Hofmeister R, Wiegmann K, Korherr C, Bernardo K, Kronke M,<br />
Falk W. Activation of acid sphingomyelinase by interleukin-1 (IL-1)<br />
requires the IL-1 receptor accessory protein. J Biol Chem<br />
1997;272:27730-6<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-APP (Aβ-NT)<br />
CATALOG NO.: 2133<br />
BACKGROUND:<br />
Accumulation of the amyloid-β peptide (Aβ) in the cerebral<br />
cortex is a critical event in the pathogenesis of Alzheimer’s<br />
disease. The β−amyloid protein precursor (APP) is cleaved<br />
by β−secretase, producing a soluble derivative of the protein<br />
and a membrane anchored 99-amino acid carboxy-terminal<br />
fragment (C99). The C99 fragment serves as substrate for<br />
γ−secretase to generate the 4 kDa amyloid-β peptide (Aβ),<br />
which is deposited in the brains of all suffers of Alzheimer’s<br />
disease.<br />
SOURCE:<br />
Rabbit anti-APP (Aβ-NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 653 to 662<br />
of human amyloid A4 protein precursor (APP) (1) or 1 to 10<br />
of the 4K Aβ peptide generated by β− and γ−secretases (2).<br />
The peptide sequences are identical to those of rabbit, pig,<br />
bovine, guinea pig, and chicken.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of APP<br />
and Aβ peptide by Western blot at 1:500 to 1:1000 dilution.<br />
Murine brain lysate can be used as positive control. It is<br />
human, mouse, and rat reactive. This antibody is for<br />
research use only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 100 µg / 500 µl, is available for<br />
competition studies (<strong>Catalog</strong> No. 2133P).<br />
Murine brain tissue lysate, 500 µg / 200 µl, is available for<br />
positive control (<strong>Catalog</strong> No. 1255).<br />
Western blot analysis of APP<br />
in human (A), mouse (B), and<br />
rat (C) brain tissue lysates<br />
with anti-APP (Aβ-NT) at<br />
1:500 dilution.<br />
REFERENCES:<br />
1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D,<br />
Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new<br />
A4 amyloid mRNA contains a domain homologous to serine<br />
proteinase inhibitors. Nature 1988;331:525-7.<br />
2. Selkoe D.J. Cell biology of the amyloid beta-protein precursor<br />
and the mechanism of Alzheimer's disease. Annu Rev Cell Biol<br />
1994;10:373-403<br />
(RD1000)
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Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-APP (CT)<br />
CATALOG NO.: 2136<br />
BACKGROUND:<br />
Accumulation of the amyloid-β peptide (Aβ) in the cerebral<br />
cortex is a critical event in the pathogenesis of Alzheimer’s<br />
disease. The β−amyloid protein precursor (APP) is cleaved<br />
by β−secretase, producing a soluble derivative of the protein<br />
and a membrane anchored 99-amino acid carboxy-terminal<br />
fragment (C99). The C99 fragment serves as substrate for<br />
γ−secretase to generate the 4 kDa amyloid-β peptide (Aβ),<br />
which is deposited in the brains of all suffers of Alzheimer’s<br />
disease.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
Western blot analysis of<br />
APP in mouse (M) and rat<br />
(R) brain tissue lysates with<br />
anti-APP (CT) at 1:500<br />
dilution.<br />
SOURCE:<br />
Rabbit anti-APP (CT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 737 to 751 of human<br />
amyloid A4 protein precursor (APP) (1) or 85 to 99 of the<br />
C99 fragment generated by β− secretase cleavage (2). The<br />
peptide sequences are identical to those of monkey, mouse,<br />
rat, chicken, and a variety of other species.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of APP<br />
and the C99 fragment by Western blot at 1:500 to 1:1000<br />
dilution. Murine brain lysate can be used as positive control.<br />
It is human, mouse, and rat reactive. This antibody is for<br />
research use only.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 100 µg / 500 µl, is available for<br />
competition studies (<strong>Catalog</strong> No. 2136P).<br />
Murine brain tissue lysate, 500 µg / 200 µl, is available for<br />
positive control (<strong>Catalog</strong> No. 1255).<br />
REFERENCES:<br />
1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D,<br />
Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new<br />
A4 amyloid mRNA contains a domain homologous to serine<br />
proteinase inhibitors. Nature 1988;331:525-7<br />
2. Selkoe D.J. Cell biology of the amyloid beta-protein precursor<br />
and the mechanism of Alzheimer's disease. Annu Rev Cell Biol<br />
1994;10:373-403<br />
(RD1299)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DcR3 (NT) TR6<br />
CATALOG No.: 2140<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand in the TNF family through their death domain<br />
containing receptors. Several novel members in the TNFR<br />
family including DR3, DR4, DR5, and DR6 were recently<br />
discovered and function as cell death receptors. Two decoy<br />
receptors, DcR1 and DcR2, were recently identified to<br />
compete with DR4 and DR5 for their ligand TRAIL binding. A<br />
novel decoy receptor was more recently discovered and<br />
designated DcR3 and TR6, respectively, (1,2). Unlike DcR1<br />
and DcR2, DcR3 is a soluble rather than a membrane<br />
associated molecule. DcR3 binds to FasL and LIGHT and<br />
inhibits FasL and LIGHT induced apoptosis (1,2). DcR3<br />
transcript is expressed in a number of lung and colon<br />
carcinomas and in some normal tissues.<br />
SOURCE:<br />
Rabbit anti-DcR3/TR6 (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 31 to 46 of<br />
human DcR3 precursor (1,2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DcR3<br />
expression by Western blot at 1:500 to 1:1000 dilution.<br />
Tissue lysate of human heart can be used as positive control<br />
and an approximately 33 kDa band can be detected. It is<br />
human, mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at -20°C. Stable for one year at 2-8°C.<br />
Western blot analysis of DcR3<br />
in human heart (H), brain (B),<br />
and kidney (K) tissue lysates<br />
with anti-DcR3 (NT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd<br />
P, Huang A, Donahue CJ, Sherwood SW, Baldwin DT, Godowski<br />
PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD,<br />
Botstein D, Ashkenazi A. Genomic amplification of a decoy<br />
receptor for Fas ligand in lung and colon cancer. Nature<br />
1998;396:699-703<br />
2. Yu KY, Kwon B, Ni J, Zhai Y, Ebner R, Kwon BS. A newly<br />
identified member of tumor necrosis factor receptor superfamily<br />
(TR6) suppresses LIGHT-mediated apoptosis. J Biol Chem<br />
1999;274:13733-6<br />
(RD1299)
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Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-SODD (NT)<br />
CATALOG No.: 2143<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand of the TNF family through their death domain<br />
containing receptors, TNF-R1 and Fas. Several novel death<br />
receptors including DR3, DR4, DR5, and DR6 were recently<br />
identified. Cell death signal is transduced by death domain<br />
containing adapter molecules through the interaction with<br />
death domain of these death receptors. A novel TNF-R1<br />
interacting protein was recently identified and designated<br />
SODD for silencer of death domains (1). SODD associates<br />
with the death domain of TNF-R1 and prevents constitutive<br />
activation of TNF-R1 signaling. TNF treatment releases<br />
SODD and permits adapter molecules such as TRADD<br />
recruiting to the active TNF-R1 complex, which activates<br />
TNF signaling pathways. SODD also interacts with DR3.<br />
SODD is ubiquitously expressed in human tissues and cell<br />
lines.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of SODD<br />
in HeLa (1,3) and THP-1 (2,4)<br />
whole cell lysates in the<br />
absence (1,2) or presence (3,4)<br />
of blocking peptide (<strong>Catalog</strong> no.<br />
2143P) with anti-SODD (NT) at<br />
1:500 dilution.<br />
REFERENCES:<br />
1. Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of<br />
constitutive TNF receptor 1 signaling by silencer of death domains.<br />
Science 1999;283:543-6<br />
(RD1299)<br />
SOURCE:<br />
Rabbit anti-SODD (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 2 to 16 of<br />
human SODD (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of SODD<br />
by Western blot at 1:500 to 1:2000 dilution. HeLa and THP-1<br />
whole cell lysate can be used as positive control and an<br />
approximately 60 kDa band can be detected. It is human,<br />
mouse, and rat reactive. For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DRAK1 (NT)<br />
CATALOG No.: 2147<br />
BACKGROUND:<br />
Apoptosis is mediated by death domain containing adapter<br />
molecules and a caspase family of proteases. Certain<br />
serine/threonine protein kinases, such as ASK-1 and RIP,<br />
are mediators of apoptosis. Two novel serine/threonine<br />
kinases that induce apoptosis were recently identified and<br />
designated DRAK1 and DRAK2 for DAP kinase-related<br />
apoptosis-inducing protein kinases (1). DRAKs contain an N-<br />
terminal kinase domain and a C-terminal regulation domain.<br />
Overexpression of DRAK1 induces apoptosis. DRAKs have<br />
high sequence homology to DAP and ZIP kinases, and they<br />
represent a novel family of serine/threonine kinases, which<br />
mediates apoptosis through their catalytic activities. DRAK1<br />
is located in nucleus and the messenger RNA was<br />
ubiquitously expressed in human tissues (1).<br />
SOURCE:<br />
Rabbit anti-DRAK1 (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 5 to 19 of<br />
human DRAK1 (1).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
DRAK1 in MOLT4 (A),<br />
A431 (B), and 3T3 (C)<br />
whole cell lysates with<br />
anti-DRAK1 (2147) at<br />
1:500 dilution.<br />
REFERENCES:<br />
1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine<br />
kinases related to death-associated protein kinase that trigger<br />
apoptosis. J Biol Chem 1998;273:29066-71<br />
(WD1299)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DRAK1<br />
by Western blot at 1:500 to 1:1000 dilution. A431 or MOLT4<br />
whole cell lysate can be used as positive control and an<br />
approximately 50 kDa band can be detected. It is human,<br />
mouse, and rat reactive, and has no cross responses to<br />
DRAK2, DAP or ZIP kinases. For research use only.
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Anti-DRAK2 (CT)<br />
CATALOG No.: 2149<br />
BACKGROUND:<br />
Apoptosis is mediated by death domain containing adapter<br />
molecules and a caspase family of proteases. Certain<br />
serine/threonine protein kinases, such as ASK-1 and RIP,<br />
are mediators of apoptosis. Two novel serine/threonine<br />
kinases that induce apoptosis were recently identified and<br />
designated DRAK1 and DRAK2 (for DAP kinase-related<br />
apoptosis-inducing protein kinases) (1). DRAKs contain an<br />
N-terminal kinase domain and a C-terminal regulation<br />
domain. Overexpression of DRAK2 induces apoptosis.<br />
DRAKs have high sequence homology to DAP and ZIP<br />
kinases, and they represent a novel family of<br />
serine/threonine kinases, which mediates apoptosis through<br />
their catalytic activities. DRAK2 is located in nucleus and the<br />
messenger RNA was ubiquitously expressed in human<br />
tissues (1).<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
DRAK2 in Jurkat (1,3) and<br />
Raji (2,4) cell lysate in the<br />
absence (1,2) or presence<br />
(3,4) of blocking peptide with<br />
anti-DRAK2 (CT) at 1:500<br />
dilution.<br />
REFERENCES:<br />
1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine<br />
kinases related to death-associated protein kinase that trigger<br />
apoptosis. J Biol Chem 1998;273:29066-71<br />
(RD1299)<br />
SOURCE:<br />
Rabbit anti-DRAK2 (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 351 to 365<br />
of human DRAK2 (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DRAK2<br />
by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate<br />
from Jurkat cells can be used as positive control and an<br />
approximately 45 kDa band can be detected. It has no cross<br />
responses to DAP or ZIP kinases. The approximately 70 kDa<br />
band is probably non-related to DRAK2 although it is peptide<br />
blockable. For research use only.
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Anti-DFF40 (NT) CPAN, hCAD<br />
CATALOG No.: 2153<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A mouse DNase<br />
that causes DNA fragmentation was identified recently and<br />
designated CAD for caspase activated deoxyribonuclease<br />
(1,2). The human homologue of mouse CAD was more<br />
recently identified by three groups independently and termed<br />
CPAN, DFF40, and human CAD, respectively, (3-5).<br />
DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6)<br />
and forms complex with DFF40/CAD. Upon cleavage of<br />
DFF45/ICAD by activated caspase, DFF40/CAD is released<br />
and activated and eventually causes the degradation of DNA<br />
in the nuclei. Activation of DFF40/CAD, which causes DNA<br />
degradation, is the hallmark of apoptotic cell death.<br />
SOURCE:<br />
Rabbit anti-DFF40 (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 3 to 18 of<br />
human DFF40 (4, 5).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DFF40<br />
by Western blot at 1:500 to 1:2000 dilution. K562 or Jurkat<br />
whole cell lysate can be used as positive control and a 40<br />
kDa band can be detected. It is human and mouse reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100 µg<br />
in 200 µl of PBS containing 0.02% sodium azide. Store at<br />
4°C, stable for one year.<br />
Western blot analysis of<br />
DFF40 in Jurkat (J) and<br />
K562 (K) whole cell lysate<br />
with anti-DFF40 (NT) at<br />
1:500 dilution.<br />
REFERENCES:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
3. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L,<br />
Williams LT. CPAN, a human nuclease regulated by the caspasesensitive<br />
inhibitor DFF45. Curr Biol 1998;8:537-40<br />
4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H,<br />
Nagata S. Molecular cloning and characterization of human<br />
caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-<br />
8<br />
6. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
(WD1299)
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Anti-DFF40 (IN) CPAN, hCAD<br />
CATALOG No.: 2155<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain containing adapter<br />
molecules and members of the caspase family of proteases.<br />
These death signals finally cause the degradation of<br />
chromosomal DNA by activated DNase. A mouse DNase<br />
that causes DNA fragmentation was identified recently and<br />
designated CAD for caspase activated deoxyribonuclease<br />
(1,2). The human homologue of mouse CAD was more<br />
recently identified by three groups independently and termed<br />
CPAN, DFF40, and human CAD, respectively, (3-5).<br />
DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6)<br />
and forms complex with DFF40/CAD. Upon cleavage of<br />
DFF45/ICAD by activated caspase, DFF40/CAD is released<br />
and activated and eventually causes the degradation of DNA<br />
in the nuclei. Activation of DFF40/CAD, which causes DNA<br />
degradation, is the hallmark of apoptotic cell death.<br />
SOURCE:<br />
Rabbit anti-DFF40 (IN) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 203 to 218<br />
of human DFF40 (4, 5).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DFF40<br />
/CAD by Western blot at 1:500 dilution. K562 or Jurkat whole<br />
cell lysate can be used as positive control and a 40 kDa<br />
band can be detected. It is human, mouse, and rat reactive,<br />
For research use only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100<br />
µg in 200 µl of PBS containing 0.02% sodium azide. Store<br />
at 4°C, stable for one year.<br />
REFERENCES:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A,<br />
Nagata S. A caspase-activated DNase that degrades DNA during<br />
apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50<br />
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in<br />
CAD activation and DNA degradation during apoptosis. Nature<br />
1998;391:96-99<br />
3. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L,<br />
Williams LT. CPAN, a human nuclease regulated by the caspasesensitive<br />
inhibitor DFF45. Curr Biol 1998;8:537-40<br />
4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The<br />
40-kDa subunit of DNA fragmentation factor induces DNA<br />
fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H,<br />
Nagata S. Molecular cloning + characterization of human caspaseactivated<br />
DNase. Proc Natl Acad Sci USA 1998;95:9123-8<br />
6. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric<br />
protein that functions downstream of caspase-3 to trigger DNA<br />
fragmentation during apoptosis. Cell 1997;89:175-184<br />
(WD0300)
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DR6 (NT)<br />
CATALOG No.: 2157<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand of the TNF family through their death domain<br />
containing receptors, TNF-R1 and Fas. Several novel death<br />
receptors including DR3, DR4, and DR5 were recently<br />
identified. A new death domain containing receptor in the<br />
TNFR family was cloned recently and termed DR6 for death<br />
receptor-6 (1). Like TNF-R1, DR6 interacts with death<br />
domain containing adapter molecule TRADD.<br />
Overexpression of DR6 induces apoptosis and activates NFκB<br />
and JNK. DR6 is widely expressed in human tissues and<br />
cell lines (1). The ligand for DR6 has not been identified.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
Western blot analysis of DR6<br />
in K562 (1,3) and Raji (2,4)<br />
whole cell lysate in the<br />
absence (1,2) or presence<br />
(3,4) of blocking peptide<br />
(<strong>Catalog</strong> no. 2157P) with anti-<br />
DR6 (NT) at 1:500 dilution.<br />
SOURCE:<br />
Rabbit anti-DR6 (NT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 42 to 56 of human<br />
DR6 precursor (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DR6 by<br />
Western blot at 1:500 to 1:2000 dilution. HeLa and K562<br />
whole cell lysate can be used as positive control and an<br />
approximately 68 kDa band can be detected. For research<br />
use only.<br />
REFERENCES:<br />
1. Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, Vincenz C,<br />
Aggarwal BB, Ni J, Dixit VM. Identification and functional<br />
characterization of DR6, a novel death domain-containing TNF<br />
receptor. FEBS Lett 1998;431:351-6<br />
(RD1299)
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Bcl10 (NT) CIPER, mE10, CARMEN, CLAP<br />
CATALOG No.: 2161<br />
BACKGROUND:<br />
Apoptosis is related to many diseases including cancer. Cell<br />
death signals are transduced by death domain (DD) and<br />
caspase recruitment domain (CARD) containing molecules<br />
and a caspase family of proteases. CARD containing cell<br />
death regulators include ARC, RAIDD, Apaf-1, caspase-9,<br />
and caspase-2. A novel CARD containing protein was<br />
recently identified by several groups and designated Bcl10,<br />
CIPER, mE10, CARMEN, CLAP (1-6). Bcl10 is a cellular<br />
homolog of the equine herpesvirus-2 E-10 gene.<br />
Overexpression of Bcl10 induces JNK, p38, and NF-κB<br />
activation (1,2,4,6). Bcl10 interacts with caspase-9 and<br />
enhances pro-caspase-9 processing (3) and induces<br />
apoptosis through caspase-9 activation (3.6). Bcl10 exhibits<br />
a variety of mutations in MALT lymphomas and in B and T<br />
cell lineage lymphomas indicating that it may be commonly<br />
involved in the pathogenesis of human malignancy (1,5).<br />
Bcl10 is expressed in many human and murine tissues and<br />
cell lines (2-6).<br />
SOURCE:<br />
Rabbit anti-BCL10 (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 5 to 19 of<br />
human origin (1-6).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of BCL10<br />
by Western blot at 1:500 dilution. Raji whole cell lysate can<br />
be used as positive control and an approximately 31 kDa<br />
band can be detected. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2161P).<br />
Raji cell lysate, 200 µg/100 µl, is available for positive<br />
control (<strong>Catalog</strong> No. 1207).<br />
Western blot analysis of Bcl-10<br />
in Raji whole cell lysate in the<br />
absence (A) or presence (B) of<br />
peptide (2161P) with anti-Bcl-10<br />
(NT) at 1:500 dilution.<br />
REFERENCES:<br />
1. Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in<br />
t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple<br />
tumor types. Cell 1999;96(1):35-45<br />
2. Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF kappaBactivating<br />
protein containing a caspase recruitment domain with<br />
homology to Herpesvirus-2 protein E10. J Biol Chem<br />
1999;274(15):9955-61<br />
3. Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel<br />
caspase recruitment domain-containing proapoptotic molecule. J<br />
Biol Chem 1999;274(15):10287-92<br />
4. Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2<br />
E10 gene product, but not its cellular homologue, activates NFkappaB<br />
transcription factor and c-Jun N-terminal kinase. J Biol<br />
Chem 1999;274(15):9962-8<br />
5. Zhang Q, Siebert R, Yan M, Hinzmann B, et al. Inactivating<br />
mutations and overexpression of BCL10, a caspase recruitment<br />
domain-containing gene, in MALT lymphoma with t(1;14)(p22;q32).<br />
Nat Genet 1999;22(1):63-8<br />
6. Srinivasula SM, Ahmad M, Lin JH, et al. CLAP, a novel caspase<br />
recruitment domain-containing protein in the tumor necrosis factor<br />
receptor pathway, regulates NF-kappaB activation and apoptosis. J<br />
Biol Chem 1999;274(25):17946-54<br />
(WD0300)
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Toll Free: 888-513-9525<br />
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Anti-DcR1 (ED) TRAIL-R3, TRID, LIT<br />
CATALOG No.: 2179<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand in the TNF family through their death domain<br />
containing receptors. TRAIL/Apo2L is a new member of the<br />
TNF family and induces apoptosis of a variety of tumor cell<br />
lines. DR4 and DR5 are the recently identified functional<br />
receptors for TRAIL (1-3). Two decoy receptors for TRAIL<br />
have been identified and designated DcR1/TRID/TRAIL-<br />
R3/LIT (2-7) and DcR2/TRAIL-R4/TRUNDD (8-10). DcR1<br />
has extracellular TRAIL-binding domain but lacks<br />
intracellular signaling domain. It is a glycophospholipidanchored<br />
cell surface protein. DcR1 transcripts were<br />
expressed in many normal human tissues but not in most<br />
cancer cell lines (2,3). Overexpression of DcR1 did not<br />
induce apoptosis, but attenuated TRAIL-induced apoptosis<br />
(2,3).<br />
SOURCE:<br />
Rabbit anti-DcR1 (ED) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 149 to 167<br />
at extracellular domain (ED) of human DcR1 precursor (2-3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DcR1<br />
by Western blot at 1:500 to 1:1000 dilution. HeLa cell lysate<br />
can be used as positive control and an approximate 65 kDa<br />
band can be detected (6). It is human, mouse, and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as chromatography purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at -20°C.<br />
Stable for one year at 2-8°C.<br />
Western blot analysis of DcR1<br />
in HeLa whole cell lysate with<br />
anti-DcR1 (ED) at 1:500 dilution.<br />
REFERENCES:<br />
1. Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic<br />
ligand TRAIL. Science; 1997;276:111-113<br />
2. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death<br />
domain-containing receptor for TRAIL. Science 1997;277:815-8<br />
3. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced<br />
apoptosis by a family of signaling and decoy receptors. Science<br />
1997;277:818-21<br />
4. Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and<br />
characterization of TRAIL-R3, a novel member of the emerging TRAIL<br />
receptor family. J Exp Med 1997;186(7):1165-70<br />
5. MacFarlane M, Ahmad M, Srinivasula SM, et al. Identification and<br />
molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J<br />
Biol Chem 1997;272(41):25417-20<br />
6. Schneider P, Bodmer JL, Thome M, Hofmann K, Holler N, Tschopp J.<br />
Characterization of two receptors for TRAIL. FEBS Lett 1997;416(3):329-34<br />
7. Mongkolsapaya J, Cowper AE, Xu XN, et al. Lymphocyte inhibitor of<br />
TRAIL (TNF-related apoptosis -inducing ligand): a new receptor protecting<br />
lymphocytes from the death ligand TRAIL. J Immunol 1998;160(1):3-6<br />
8. Marsters SA, Sheridan JP, Pitti RM, et al. A novel receptor for<br />
Apo2L/TRAIL contains a truncated death domain. Curr Biol 1997;7:1003-6<br />
9. Degli-Esposti MA, Dougall WC, Smolak PJ, et al. The novel receptor<br />
TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated<br />
apoptosis, yet retains an incomplete death domain. Immunity 1997;7:813-20<br />
10. Pan G, Ni J, Yu G, et al. TRUNDD, a new member of the TRAIL<br />
receptor family that antagonizes TRAIL signalling. FEBS Lett 1998;424:41-5<br />
(RD0800)
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Poway, CA 92064<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-RICK (CT) RIP2, CARDIAK<br />
CATALOG No.: 2183<br />
BACKGROUND:<br />
Apoptosis is mediated by death domain (DD) and/or caspase<br />
recruitment domain (CARD) containing molecules and a<br />
caspase family of proteases. DD-containing serine/threonine<br />
kinase RIP regulates Fas-induced apoptosis. A novel CARDcontaining<br />
serine/threonine kinase was recently identified<br />
and designated RICK/RIP2/CARDIAK for RIP-like interacting<br />
CLARP kinase, receptor interacting protein-2, and CARDcontaining<br />
ICE associated kinase, respectively, (1-3). RICK<br />
contains an N-terminal kinase catalytic domain and a C-<br />
terminal CARD domain. Overexpression of RICK induced<br />
apoptosis and activation of NF-κB and JNK. RICK interacts<br />
with members of the TRAF family, CLARP and caspase-1.<br />
Thus, RICK represents a novel kinase that regulates TNF<br />
and Fas induced-apoptosis and that is involved in the<br />
generation of proinflammatory cytokine IL-1β. The<br />
messenger RNA of RICK is expressed in multiple human<br />
tissues (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of RICK<br />
by Western blot at 1:500 dilution. K562 or 3T3 whole cell<br />
lysate can be used as positive control and an approximately<br />
60 kDa band can be detected. It is human, mouse, and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
REFERENCES:<br />
1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a<br />
novel protein kinase containing a caspase recruitment domain,<br />
interacts with CLARP and regulates CD95-mediated apoptosis. J<br />
Biol Chem 1998;273:12296-300<br />
2. McCarthy JV, Ni J, Dixit VM. RIP2 is a novel NF-kappaBactivating<br />
and cell death-inducing kinase. J Biol Chem<br />
1998;273:16968-75<br />
SOURCE:<br />
Rabbit anti-RICK (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 508 to 522<br />
of human origin (1).<br />
3. Thome M, Hofmann K, Burns K, Martinon F, Bodmer JL,<br />
Mattmann C, Tschopp J. Identification of CARDIAK, a RIP-like<br />
kinase that associates with caspase-1. Curr Biol 1998;8:885-8<br />
(WD0300)
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Poway, CA 92064<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-ARC (NT)<br />
CATALOG No.: 2185<br />
BACKGROUND:<br />
Apoptosis is regulated by death domain (DD) and/or<br />
caspase recruitment domain (CARD) containing molecules<br />
and a caspase family of proteases. CARD containing cell<br />
death regulators include RAIDD, RICK BCL10, Apaf-1,<br />
caspase-9, and caspase-2. A novel CARD domain<br />
containing protein was recently identified and designated<br />
ARC for apoptosis repressor with CARD (1). ARC interacts<br />
with caspase-2 and –8 and inhibits enzymatic activity of<br />
caspase-8. ARC suppresses apoptosis induced by cell death<br />
adapters FADD and TRADD and by cell death receptors<br />
Fas, TNFR-1, and DR3. The messenger RNA of ARC is<br />
primarily expressed in skeletal muscle and cardiac tissue (1).<br />
SOURCE:<br />
Rabbit anti-ARC (NT) polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 2 to 18 of human<br />
origin (1). These sequences are identical to those of human<br />
nuclear protein Nop30 (2) and differ from those of the rat<br />
homolog of ARC by one amino acid (3)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of ARC<br />
by Western blot at 1:500 dilution. Whole cell lysate from<br />
HeLa cells can be used as positive control and an<br />
approximately 25 kDa band can be detected. It is human,<br />
mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2185P).<br />
HeLa cell lysate, 200 µg/100 µl, is available for positive<br />
control (<strong>Catalog</strong> No. 1201).<br />
Western blot analysis of ARC in<br />
HeLa whole cell lysates with anti-<br />
ARC (NT) at 1:500 dilution.<br />
REFERENCES:<br />
1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of<br />
apoptosis expressed in skeletal muscle and heart that interacts<br />
selectively with caspases. Proc Natl Acad Sci USA 1998;95:5156-<br />
60<br />
2. Stoss O, Schwaiger FW, Cooper TA, Stamm S. Alternative<br />
splicing determines the intracellular localization of the novel<br />
nuclear protein Nop30 and its interaction with the splicing factor<br />
SRp30c. J Biol Chem 1999;274(16):10951-62<br />
3. Geertman R, McMahon A, Sabban EL. Cloning and<br />
characterization of cDNAs for novel proteins with glutamic acidproline<br />
dipeptide tandem repeats. Biochim Biophys Acta<br />
1996;1306(2-3):147-52<br />
(WD 0300)
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Anti-CX 3 CR1 (EL) V28, CMKBRL1<br />
CATALOG No.: 2201<br />
BACKGROUND:<br />
CX 3 CR1 is one of the chemokine receptors that are required<br />
as coreceptors for HIV infection. The genes encoding<br />
human, mouse, and rat CX 3 CR1 were cloned and<br />
designated V28 and CMKBRL1, CX 3 CR1, and RBS11,<br />
respectively, (1-4). The encoded seven transmembrane<br />
protein was recently identified as the receptor for a novel<br />
transmembrane molecule, fractalkine, and renamed CX 3 CR1<br />
(5). Recently, CX 3 CR1 was found to serve as a coreceptor<br />
for HIV-1 and HIV-2 envelope fusion and virus infection,<br />
which can be inhibited by fractokine (6). CX 3 CR1 mediates<br />
leukocyte migration and adhesion (5). CX 3 CR1 is expressed<br />
in a variety of human tissues and cell lines (2).<br />
SOURCE:<br />
Rabbit anti-CX 3 CR1 polyclonal antibody was raised against<br />
a peptide corresponding to amino acids 175 to 189 of human<br />
CX 3 CR1 (1,2). The sequence is identical to that of rat<br />
CX 3 CR1 and differs from that of mouse CX 3 CR1 by one<br />
amino acid (3,4).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
CX 3 CR1 by Western blot at 1:500 dilution. THP-1 cell lysate<br />
can be used as positive control and an approximately 50<br />
kDa band can be detected. For research use only.<br />
STORAGE:<br />
It is supplied as 100 µg of immunoaffinity chromatography<br />
purified IgG in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2201P).<br />
THP-1 cell lysate, 200 µg/100 µl, is available for positive<br />
control (<strong>Catalog</strong> No. 1208).<br />
Western blot analysis of<br />
CX 3 CR1 in THP-1 cell lysate in<br />
the absence (lane A) or<br />
presence (lane B) of blocking<br />
peptide with anti-CX 3 CR1 (EL)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The<br />
orphan G-protein-coupled receptor-encoding gene V28 is closely related to<br />
genes for chemokine receptors and is expressed in lymphoid and neural<br />
tissues. Gene 1995;163:295-9<br />
2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization,<br />
and RNA expression of a human beta chemokine receptor-like gene. DNA<br />
Cell Biol 1995;14:673-80<br />
3. Combadiere C, Gao J, Tiffany HL, Murphy PM. Gene cloning, RNA<br />
distribution, and functional expression of mCX3CR1, a mouse chemotactic<br />
receptor for the CX3C chemokine fractalkine. Biochem Biophys Res<br />
Commun 1998;253:728-32<br />
4. Harrison JK, Barber CM, Lynch KR. cDNA cloning of a G-protein-coupled<br />
receptor expressed in rat spinal cord and brain related to chemokine<br />
receptors. Neurosci Lett 1994;169:85-9<br />
5. Imai T, Hieshima K, Haskell C, et al. Identification and molecular<br />
characterization of fractalkine receptor CX3CR1, which mediates both<br />
leukocyte migration and adhesion. Cell 1997;91:521-30<br />
6. Combadiere C, Salzwedel K, Smith ED, et al. Identification of CX3CR1. A<br />
chemotactic receptor for the human CX3C chemokine fractalkine and a<br />
fusion coreceptor for HIV-1. J Biol Chem 1998;273:23799-804 (RD0400)
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Anti-Bonzo (NT2) STRL33, TYMSTR<br />
CATALOG NO.: 2209<br />
BACKGROUND:<br />
Human immunodeficiency virus (HIV) and simian<br />
immunodeficiency virus (SIV) require coreceptors, in addition<br />
to CD4, to infect target cells. Some G protein-coupled<br />
receptors including CCR5, CXCR4, CCR3, and CCR2b in<br />
the chemokine receptor family have been identified as HIV<br />
coreceptors. An orphan G protein-coupled receptor was<br />
recently cloned and designated Bonzo, STRL33 and<br />
TYMSTR, and identified as HIV and SIV coreceptor (1-4).<br />
Bonzo/STRL33 serves as coreceptor for SIV, HIV-2 and<br />
HIV-1. The messenger RNA of Bonzo/STRL33 is expressed<br />
in lymphoid tissues and activated peripheral blood<br />
lymphocytes.<br />
SOURCE:<br />
Rabbit anti-Bonzo polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 11 to 25 of human<br />
Bonzo/STRL33 (1,2). The sequence of this peptide differs<br />
from those of African green monkey and pig-tailed macaque<br />
by one or two amino acids, respectively, (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Bonzo<br />
by Western blot at 1:500 dilution. Human spleen tissue<br />
lysate can be used as positive control. For research use<br />
only.<br />
STORAGE:<br />
It is supplied as 100 µg of immunoaffinity purified IgG in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2209P).<br />
Human spleen tissue lysate, 200 µg/100 µl, is available for<br />
positive control (<strong>Catalog</strong> No. 1306).<br />
Western blot analysis of<br />
Bonzo in human spleen tissue<br />
lysate in the absence (lane A)<br />
or presence (lane B) of<br />
peptide with anti-Bonzo (NT2)<br />
at 1:500 dilution.<br />
REFERENCES:<br />
1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR.<br />
Expression cloning of new receptors used by simian and human<br />
immunodeficiency viruses. Nature 1997;388:296-300<br />
2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber<br />
JM. STRL33, A novel chemokine receptor-like protein, functions as<br />
a fusion cofactor for both macrophage-tropic and T cell line-tropic<br />
HIV-1. J Exp Med 1997;185:2015-23<br />
3. Alkhatib G, Liao F, Berger EA, Farber JM, Peden KW. A new<br />
SIV co-receptor, STRL33. Nature 1997;388:238<br />
4. Loetscher M, Amara A, Oberlin E, Brass N, Legler D, Loetscher<br />
P, D'Apuzzo M, Meese E, Rousset D, Virelizier JL, Baggiolini M,<br />
Arenzana-Seisdedos F, Moser B. TYMSTR, a putative chemokine<br />
receptor selectively expressed in activated T cells, exhibits HIV -1<br />
coreceptor function. Curr Biol 1997;7:652-60 (RD0400)
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Anti-IRAK2 (CT)<br />
CATALOG No.: 2213<br />
BACKGROUND:<br />
The pro-inflammatory cytokine IL-1 induces cellular response<br />
through two subunits of its receptor, IL-1 receptor I (IL-1RI)<br />
and IL-1 receptor accessory protein (IL-1RAcP). IL-1<br />
receptor-associated kinase (IRAK) mediates activation of<br />
NF-κB (1), which is a pivotal transcription factor mediating<br />
inflammatory and immune response. A novel member in the<br />
IRAK/Pelle family was recently identified and designated<br />
IRAK2 (2). Both IRAK and IRAK2 recruit to the subunits of<br />
the IL-1R complex after IL-1 binding and lead to NF-κB<br />
activation. IRAKs also associate with Toll-like receptor (TLR)<br />
and the dominant negative mutants of IRAKs inhibit LPSinduced<br />
NF-κB activation (3,4). Members in IRAK/Pelle<br />
family play a central role in IL-1R and TLR mediated<br />
inflammatory responses to cytokine IL-1 and LPS. IRAK2 is<br />
expressed in a variety of human tissues.<br />
SOURCE:<br />
Rabbit anti-IRAK2 (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 571 to 590<br />
of human IRAK2 (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IRAK2<br />
by Western blot at 1:500 to 1:1000 dilution. K562 whole cell<br />
lysate can be used as positive control and a 65 kDa band<br />
can be detected. Anti-IRAK2 has no cross response to IRAK.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as chromatography purified IgG, 100 µg in<br />
200 µl of PBS containing 0.02% sodium azide. Store at<br />
4°C, stable for one year.<br />
Western blot analysis of<br />
IRAK2 in K562 whole cell<br />
lysate with anti-IRAK2 (CT) at<br />
1:500 dilution.<br />
REFERENCES:<br />
1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the<br />
interleukin-1 receptor. Science 1996;271:1128-31.<br />
2. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member<br />
IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.<br />
Science 1997;278:1612-5<br />
3. Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E,<br />
Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial<br />
lipopolysaccharide activates nuclear factor-kappaB through<br />
interleukin-1 signaling mediators in cultured human dermal<br />
endothelial cells and mononuclear phagocytes. J Biol Chem<br />
1999;274:7611-4<br />
4. Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events<br />
induced by lipopolysaccharide-activated toll-like receptor 2. J<br />
Immunol 1999;163:639-43<br />
(RD0300)
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Anti-Acinus (CP)<br />
CATALOG No.: 2215<br />
BACKGROUND:<br />
Chromatin condensation and nuclear fragmentation (CCNF)<br />
is the hallmark of apoptosis. CCNF is triggered by the<br />
activation of members of caspase family, caspase activated<br />
DNase (CAD/DFF40), and several novel proteins including<br />
AIF and CIDE (1). A new inducer of chromatin condensation<br />
was recently identified and designated Acinus (for apoptotic<br />
chromatin condensation inducer in the nucleus). Acinus is<br />
cleaved by caspase-3 and an additional unknown protease<br />
generating a small active peptide p17, which causes<br />
chromatin condensation in vitro when it is added to purified<br />
nuclei. Acinus also induces apoptotic chromatin<br />
condensation in cells. Acinus is ubiquitously expressed.<br />
Three different spliced forms of Acinus have been identified<br />
in human and mouse and designated AcinusL, AcinusS and<br />
AcinusS’ (2).<br />
SOURCE:<br />
Rabbit anti-Acinus (CP) polyclonal antibody was raised<br />
against a peptide (TRTALHGVKWPQSNPK) corresponding<br />
to amino acids 1065 to 1080 of human AcinusL, 338 to 353<br />
of human AcinusS’, or 307 to 322 of human AcinusS, which<br />
are identical to those of mouse Acinus (2). The selected<br />
antigenic sequence is located near the C-terminus of the<br />
cleaved active peptide p17.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Western blot analysis of Acinus in<br />
K562 whole cell lysate with anti-<br />
Acinus (CP) at 1 µg/ml.<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2215P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death.<br />
Nature 1999 ;401:127-8.<br />
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto<br />
Y. Acinus is a caspase-3-activated protein required for apoptotic<br />
chromatin condensation. Nature 1999 401:168-73.<br />
(WD0800)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Acinus<br />
by Western blot at 0.5 to 1 µg/ml. K562 cell lysate can be<br />
used as positive control and an approximate 220 kDa band<br />
can be detected. For research use only.
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Anti-Acinus (CT)<br />
CATALOG No.: 2217<br />
BACKGROUND:<br />
Chromatin condensation and nuclear fragmentation (CCNF)<br />
is the hallmark of apoptosis. CCNF is triggered by the<br />
activation of members of caspase family, caspase activated<br />
DNase (CAD/DFF40), and several novel proteins including<br />
AIF and CIDE (1). A new inducer of chromatin condensation<br />
was recently identified and designated Acinus (for apoptotic<br />
chromatin condensation inducer in the nucleus). Acinus is<br />
cleaved by caspase-3 and an additional unknown protease<br />
generating a small active peptide p17, which causes<br />
chromatin condensation in vitro when it is added to purified<br />
nuclei. Acinus also induces apoptotic chromatin<br />
condensation in cells. Acinus is ubiquitously expressed.<br />
Three different spliced forms of Acinus have been identified<br />
in human and mouse and designated AcinusL, AcinusS and<br />
AcinusS’ (2).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
Acinus in K562 whole cell<br />
lysate with anti-Acinus (CT) at<br />
0.5 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2217P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
SOURCE:<br />
Rabbit anti-Acinus (CT) polyclonal antibody was raised<br />
against a peptide (SRSTPVRDRGGRR) corresponding to<br />
amino acids 1329 to 1341 of human AcinusL, 602 to 614 of<br />
human AcinusS’, or 571 to 583 of human AcinusS, which are<br />
identical to those of mouse Acinus (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Acinus<br />
by Western blot at 0.25 to 0.5 µg/ml. K562 cell lysate can be<br />
used as positive control and an approximate 220 kDa band<br />
can be detected. For research use only.<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death.<br />
Nature 1999 ;401:127-8.<br />
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto<br />
Y. Acinus is a caspase-3-activated protein required for apoptotic<br />
chromatin condensation. Nature 1999 401:168-73.<br />
(WD0800)
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Anti-Acinus (IN)<br />
CATALOG No.: 2219<br />
BACKGROUND:<br />
Chromatin condensation and nuclear fragmentation (CCNF)<br />
is the hallmark of apoptosis. CCNF is triggered by the<br />
activation of members of caspase family, caspase activated<br />
DNase (CAD/DFF40), and several novel proteins including<br />
AIF and CIDE (1). A new inducer of chromatin condensation<br />
was recently identified and designated Acinus (for apoptotic<br />
chromatin condensation inducer in the nucleus). Acinus is<br />
cleaved by caspase-3 and an additional unknown protease<br />
generating a small active peptide p17, which causes<br />
chromatin condensation in vitro when it is added to purified<br />
nuclei. Acinus also induces apoptotic chromatin<br />
condensation in cells. Acinus is ubiquitously expressed.<br />
Three different spliced forms of Acinus have been identified<br />
in human and mouse and designated AcinusL, AcinusS and<br />
AcinusS’ (2).<br />
SOURCE:<br />
Rabbit anti-Acinus (IN) polyclonal antibody was raised<br />
against a peptide (DTSENRPENDVPEPP) corresponding to<br />
amino acids 775 to 789 of human AcinusL, 48 to 62 of<br />
human AcinusS’, or 17 to 31 of human AcinusS, which differ<br />
from those of mouse Acinus by one amino acid (2).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of Acinus<br />
in K562 whole cell lysate with<br />
anti-Acinus (IN) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2219P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death.<br />
Nature 1999 ;401:127-8.<br />
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto<br />
Y. Acinus is a caspase-3-activated protein required for apoptotic<br />
chromatin condensation. Nature 1999 401:168-73.<br />
(WD0800)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Acinus<br />
by Western blot at 0.5 to 1 µg/ml. K562 cell lysate can be<br />
used as positive control and an approximate 220 kDa band<br />
can be detected. For research use only.
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-BAFF (CT) BLyS, TALL-1, THANK<br />
CATALOG No.: 2221<br />
BACKGROUND:<br />
Members in the TNF superfamily regulate immune<br />
responses and induce apoptosis. A novel member in the<br />
TNF family was recently identified by several groups and<br />
designated BAFF (for B cell Activating Factor belonging to<br />
the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-<br />
1 (for TNF- and ApoL-related Leukocyte-expressed Ligand),<br />
and THANK (for TNF Homologue that Activate Apoptosis,<br />
NF-κB and c-jun N-terminal Kinase) (1-4). BAFF/BLyS was<br />
characterized as a B cell stimulator since it induced B cell<br />
proliferation and immunoglobulin secretion (1,2). Two<br />
receptors for BAFF were recently identified and designated<br />
TACI and BCMA (5). BAFF and its receptors are involved in<br />
the development of systemic lupus erythaematosus and<br />
other B cell associated autoimmune diseases (5,6). Like<br />
TNFα and TRAIL, THANK was shown to activate NF-κB and<br />
c-jun N-terminal kinase (JNK) and to induce apoptosis (4).<br />
SOURCE:<br />
Rabbit anti-BAFF (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 254 to 269<br />
of human BAFF (1-4).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of BAFF<br />
by Western blot at 0.25 to 1 µg/ml. It is human, mouse and<br />
rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year. Azide free antibody is<br />
available.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2221P).<br />
HL60 cell lysate, 200 µg/100 µl, is available for positive<br />
control (<strong>Catalog</strong> No. 1209).<br />
Western blot analysis of<br />
BAFF in human HL60 cell<br />
lysate (A) and murine spleen<br />
tissue lysate (B) with anti-<br />
BAFF (CT) at 1 µg/ml.<br />
REFERENCES:<br />
1. Moore PA, Belvedere O, Orr A, et al. BLyS: member of the<br />
tumor necrosis factor family and B lymphocyte stimulator. Science<br />
1999;285:260-3<br />
2. Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of<br />
the tumor necrosis factor family, stimulates B cell growth. J Exp<br />
Med 1999;189:1747-56<br />
3. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the<br />
TNF family that is down-regulated by mitogens. J Leukoc Biol<br />
1999;65:680-3<br />
4. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB.<br />
Identification and characterization of a novel cytokine, THANK, a<br />
TNF homologue that activates apoptosis, nuclear factor-kappaB,<br />
and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81<br />
5. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are<br />
receptors for a TNF homologue implicated in B-cell autoimmune<br />
disease. Nature 2000;404:995-9<br />
6. Khare SD, Sarosi I, Xia XZ, et al. Severe B cell hyperplasia and<br />
autoimmune disease in TALL-1 transgenic mice. Proc Natl Acad<br />
Sci USA 2000;97:3370-5<br />
(WD0500)
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techsupport@prosci-inc.com<br />
Anti-APRIL (ED) TALL-2, TRDL-1<br />
CATALOG No.: 2223<br />
BACKGROUND:<br />
Members in the TNF superfamily regulate immune<br />
responses and induce apoptosis. A novel member in the<br />
TNF family was recently identified by several groups and<br />
designated APRIL (for a proliferation-inducing ligand), TALL-<br />
2 (for TNF- and ApoL-related Leukocyte-expressed Ligand<br />
2), and TRDL-1α (for TNF related death ligand 1α) in human<br />
and mouse (1-4). Two receptors for APRIL were recently<br />
identified and designated TACI and BCMA (5-7). APRIL<br />
stimulates B and T cell proliferation, triggers humoral<br />
immune responses, activates NF-kB, and induces cell death<br />
(1-7). APRIL and its close relative of BlyS and their<br />
receptors BCMA and TACI are involved in diseases of<br />
autoimmunity and cancer (8,9).<br />
REFERENCES:<br />
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of<br />
the tumor necrosis factor family, stimulates tumor cell growth. J<br />
Exp Med. 1998;188(6):1185-90.<br />
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the<br />
TNF family that is down-regulated by mitogens. J Leukoc Biol.<br />
1999;65(5):680-3.<br />
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor<br />
necrosis factor-like ligand, stimulates cell death. Cancer Res.<br />
2000;60(4):1021-7.<br />
4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and<br />
receptors BCMA and TACI: system for regulating humoral<br />
immunity. Nat Immunol. 2000;1(3):252-6.<br />
SOURCE:<br />
Rabbit anti-APRIL (ED) polyclonal antibody was raised<br />
against a synthetic peptide (GTGGPSQNGEGYP)<br />
corresponding to amino acids 67 to 79 of human APRIL (1-<br />
3).<br />
APPLICATION:<br />
This antibody can be used for detection of APRIL by<br />
Western blot at 4-5 µg/ml. HL60 or Jurkat cell lysate can be<br />
used as positive control and an approximately 42 kDa band<br />
can be detected. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2223P).<br />
5. Wu Y, Bressette D, Carrell JA, et a. Tumor necrosis factor<br />
(TNF) receptor superfamily member TACI is a high affinity receptor<br />
for TNF family members APRIL and BLyS. J Biol Chem.<br />
2000;275(45):35478-85.<br />
6. Marsters SA, Yan M, Pitti RM, et al. Interaction of the TNF<br />
homologues BLyS and APRIL with the TNF receptor homologues<br />
BCMA and TACI. Curr Biol. 2000;10(13):785-8.<br />
7. Rennert P, Schneider P, Cachero TG, at al. A soluble form of B<br />
cell maturation antigen, a receptor for the tumor necrosis factor<br />
family member APRIL, inhibits tumor cell growth. J Exp Med.<br />
2000;192(11):1677-84.<br />
8. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are<br />
receptors for a TNF homologue implicated in B-cell autoimmune<br />
disease. Nature 2000;404:995-9<br />
9. Ware CF. APRIL and BAFF connect autoimmunity and cancer. J<br />
Exp Med. 2000;192(11):F35-8.<br />
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Anti-DEDAF<br />
CATALOG No.: 2227<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death<br />
signals are transduced by death domain (DD) death effector<br />
domain (DED), and caspase recruitment domain (CARD)<br />
containing molecules. Several molecules including caspases<br />
and adaptor FADD contain DEDs. A novel protein that<br />
interacts with DED of caspase-8 and –10, and FADD was<br />
identified recently and designated DEDAF for DED<br />
associated factor (1). DEDAF is identical to the<br />
transcriptional repressor RYBP (2). DEDAF/RYBP is<br />
expressed in multiple tissues and cell lines. DEDAF interacts<br />
with FADD and augments the formation of<br />
CD95/FADD/capase-8 complexes at the cell membrane, and<br />
interacts with DED-containing DNA biding protein (DEDD) in<br />
the nucleus indicating it is involved in the regulation of both<br />
cytoplasmic and nuclear events of apoptosis.<br />
SOURCE:<br />
Rabbit anti-DEDAF polyclonal antibody was raised against a<br />
synthetic peptide (TPKGDMSAVNDESF) corresponding to<br />
amino acids 215 to 228 of human DEDAF (1,2). The<br />
sequence is identical to that of mouse origin (2).<br />
APPLICATION:<br />
This antibody can be used for detection of DEDAF by<br />
Western blot at 0.5 to 1 µg/ml. Human A549 or HepG2 cell<br />
lysate can be used as a positive control. A band at<br />
approximately 32 kDa can be detected. It is human, mouse,<br />
and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
Western blot analysis of DEDAF<br />
expression in human A549<br />
(lane A), HepG2 (lane B), and<br />
mouse 3T3 (lane C) cell lysates<br />
with anti-DEDAF at 1 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2227P).<br />
Human HepG2 cell lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1211).<br />
REFERENCES:<br />
1. Zheng L, Schickling O, Peter ME, Lenardo MJ. The death<br />
effector domain-associated factor (DEDAF) plays distinct<br />
regulatory roles in the nucleus and cytoplasm. J Biol Chem.<br />
2001;276(34):31945-52.<br />
2. Garcia E, Marcos-Gutierrez C, del Mar Lorente M, Moreno JC,<br />
Vidal M. RYBP, a new repressor protein that interacts with<br />
components of the mammalian Polycomb complex, and with the<br />
transcription factor YY1. EMBO J. 1999;18(12):3404-18.<br />
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Anti-Survivin (NT) TIAP/API4<br />
CATALOG No.: 2233<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, is related to many<br />
diseases, such as cancer. Apoptosis is triggered by a variety<br />
of stimuli including members in the TNF family and<br />
prevented by the inhibitor of apoptosis (IAP) proteins. IAP<br />
proteins form a conserved gene family that binds to and<br />
inhibits cell death proteases. A novel IAP protein was<br />
recently identified and designated survivin, apoptosis<br />
inhibitor 4 (API4), and TIAP (1-3). Survivin/TIAP interacted<br />
with the processed form of caspase-3 and inhibited its<br />
proteolytic activity. Survivin/TIAP is predominantly expressed<br />
in tissues of embryos, transformed cell lines, and many<br />
human cancers and lymphomas (1).<br />
SOURCE:<br />
Rabbit anti-survivin (NT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 1 to 12 of<br />
human origin (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of survivin<br />
by Western blot at 0.5 to 1 µg/ml. MOLT4 cell lysate can be<br />
used as positive control and an approximate 17 kDa band<br />
should be detected. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2233P). MOLT4 cell<br />
lysate, 200 µg at 2 mg/ml, is available for positive control<br />
(<strong>Catalog</strong> No. 1206).<br />
Western blot analysis of<br />
survivin in MOLT4 cell<br />
lysate in the absence (A) or<br />
presence (B) of blocking<br />
peptide with anti-survivin<br />
(NT) at 1 µg/ml.<br />
REFERENCES:<br />
1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene,<br />
survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-<br />
21<br />
2. Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC,<br />
Altieri DC. Control of apoptosis and mitotic spindle checkpoint by<br />
survivin. Nature 1998;396:580-4<br />
3. Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T.<br />
Expression of a murine homologue of the inhibitor of apoptosis<br />
protein is related to cell proliferation. Proc Natl Acad Sci USA<br />
1999;96:1457-62<br />
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techsupport@prosci-inc.com<br />
Anti-Survivin (CT) TIAP/API4<br />
CATALOG No.: 2235<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, is related to many<br />
diseases, such as cancer. Apoptosis is triggered by a variety<br />
of stimuli including members in the TNF family and<br />
prevented by the inhibitor of apoptosis (IAP) proteins. IAP<br />
proteins form a conserved gene family that binds to and<br />
inhibits cell death proteases. A novel IAP protein was<br />
recently identified and designated survivin, apoptosis<br />
inhibitor 4 (API4), and TIAP (1-3). Survivin/TIAP interacted<br />
with the processed form of caspase-3 and inhibited its<br />
proteolytic activity. Survivin/TIAP is predominantly expressed<br />
in tissues of embryos, transformed cell lines, and many<br />
human cancers and lymphomas (1,3).<br />
SOURCE:<br />
Rabbit anti-survivin (CT) polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 129 to 142<br />
of human origin (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of survivin<br />
by Western blot at 0.5 to 1 µg/ml. MOLT4 cell lysate can be<br />
used as positive control and an approximate 17 kDa band<br />
should be detected. For research use only.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2235P). MOLT4 cell<br />
lysate, 200 µg at 2 mg/ml, is available for positive control<br />
(<strong>Catalog</strong> No. 1206).<br />
Western blot analysis of<br />
survivin in MOLT4 cell<br />
lysate in the absence (A)<br />
or presence (B) of<br />
blocking peptide with<br />
anti-survivin (CT) at 1<br />
µg/ml.<br />
REFERENCES:<br />
1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene,<br />
survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-<br />
21<br />
2. Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC,<br />
Altieri DC. Control of apoptosis and mitotic spindle checkpoint by<br />
survivin. Nature 1998;396:580-4<br />
3. Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T.<br />
Expression of a murine homologue of the inhibitor of apoptosis<br />
protein is related to cell proliferation. Proc Natl Acad Sci USA<br />
1999;96:1457-62<br />
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Anti-Survivin (CT) TIAP/API4<br />
CATALOG No.: 2237<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, is related to many<br />
diseases, such as cancer. Apoptosis is triggered by a variety<br />
of stimuli including members in the TNF family and<br />
prevented by the inhibitor of apoptosis (IAP) proteins. IAP<br />
proteins form a conserved gene family that binds to and<br />
inhibits cell death proteases. A novel IAP protein was<br />
recently identified and designated survivin, apoptosis<br />
inhibitor 4 (API4), and TIAP (1-3). Survivin/TIAP interacted<br />
with the processed form of caspase-3 and inhibited its<br />
proteolytic activity. Survivin/TIAP is predominantly expressed<br />
in tissues of embryos, transformed cell lines, and many<br />
human cancers and lymphomas (1,3).<br />
SOURCE:<br />
Rabbit anti-survivin (CT) polyclonal antibody was raised<br />
against a synthetic peptide (AKTTRQSIEQLAA)<br />
corresponding to amino acids 128 to 140 of mouse origin (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of survivin<br />
by Western blot at 0.5 to 1 µg/ml. Mouse spleen tissue lysate<br />
can be used as positive control and an approximate 17 kDa<br />
band can be detected. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2237P). Mouse spleen<br />
lysate, 200 µg at 2 mg/ml, is available for positive control<br />
(<strong>Catalog</strong> No. 1406).<br />
Western blot analysis of<br />
survivin in mouse spleen<br />
tissue lysate with anti-survivin<br />
(CT) at 1 µg/ml.<br />
REFERENCES:<br />
1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene,<br />
survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-<br />
21<br />
2. Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC,<br />
Altieri DC. Control of apoptosis and mitotic spindle checkpoint by<br />
survivin. Nature 1998;396:580-4<br />
3. Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T.<br />
Expression of a murine homologue of the inhibitor of apoptosis<br />
protein is related to cell proliferation. Proc Natl Acad Sci USA<br />
1999;96:1457-62<br />
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techsupport@prosci-inc.com<br />
Anti-AIF (NT)<br />
CATALOG No.: 2239<br />
BACKGROUND:<br />
Apoptosis is characterized by several morphological nuclear<br />
changes including chromatin condensation and nuclear<br />
fragmentation. These changes are triggered by the activation<br />
of members of caspase family, caspase activated DNase,<br />
and several novel proteins (1). A novel gene, the product of<br />
which causes chromatin condensation and DNA<br />
fragmentation, was recently identified, cloned, and<br />
designated apoptosis inducing factor (AIF) (2). Like the<br />
critical molecules, cytochrome c and caspase-9, in<br />
apoptosis, AIF localizes in mitochondria. AIF translocates to<br />
the nucleus when apoptosis is induced and induces<br />
mitochondria to release the apoptogenic proteins<br />
cytochrome c and caspase-9. AIF induces chromatin<br />
condensation and DNA fragmentation, which are the<br />
hallmarks of apoptosis, of the isolated nucleus and the<br />
nucleus in live cells by microinjection. AIF is highly<br />
conserved between human and mouse and widely<br />
expressed (2).<br />
SOURCE:<br />
Rabbit anti-AIF (N-terminus of mature AIF) polyclonal<br />
antibody was raised against a peptide corresponding to<br />
amino acids 109 to 122 of human AIF (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of AIF by<br />
Western blot at 0.25 to 1 µg/ml. K562 cell lysate can be used<br />
as positive control and a 67 kDa band should be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
AIF in K562 cell lysate with<br />
anti-AIF (NT) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2239P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature<br />
1999;401:127-8<br />
2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular<br />
characterization of mitochondrial apoptosis-inducing factor. Nature<br />
1999;397:441-6<br />
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techsupport@prosci-inc.com<br />
Anti-Acinus (NP)<br />
CATALOG No.: 2241<br />
BACKGROUND:<br />
Chromatin condensation and nuclear fragmentation (CCNF)<br />
is the hallmark of apoptosis. CCNF is triggered by the<br />
activation of members of caspase family, caspase activated<br />
DNase (CAD/DFF40), and several novel proteins including<br />
AIF and CIDE (1). A new inducer of chromatin condensation<br />
was recently identified and designated Acinus (for apoptotic<br />
chromatin condensation inducer in the nucleus). Acinus is<br />
cleaved by caspase-3 and an additional unknown protease<br />
generating a small active peptide p17, which causes<br />
chromatin condensation in vitro when it is added to purified<br />
nuclei. Acinus also induces apoptotic chromatin<br />
condensation in cells. Acinus is ubiquitously expressed.<br />
Three different spliced forms of Acinus have been identified<br />
in human and mouse and designated AcinusL, AcinusS and<br />
AcinusS’ (2).<br />
SOURCE:<br />
Rabbit anti-Acinus (NP) polyclonal antibody was raised<br />
against a peptide (DDPVRTAQVPSPPRGK) corresponding<br />
to amino acids 994 to 1009 of human AcinusL, 267 to 282 of<br />
human AcinusS’, or 236 to 251 of human AcinusS, which are<br />
identical to those of mouse Acinus (2). The selected<br />
antigenic sequence is located near the N-terminus of active<br />
peptide p17.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
Acinus in K562 whole cell<br />
lysate with anti-Acinus (NP)<br />
at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2241P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death.<br />
Nature 1999 ;401:127-8.<br />
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto<br />
Y. Acinus is a caspase-3-activated protein required for apoptotic<br />
chromatin condensation. Nature 1999 401:168-73.<br />
(WD0800)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Acinus<br />
by Western blot at 0.5 to 1 µg/ml. K562 cell lysate can be<br />
used as positive control and an approximate 220 kDa band<br />
can be detected. For research use only.
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Anti-Caspase-13 / ERICE<br />
CATALOG No.: 2245<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and induced by a<br />
family of cell death receptors and their ligands. Cell death is<br />
finally caused by members of the caspase family of<br />
proteases and caspase activated DNases. A novel member<br />
in the caspase family was recently identified and designated<br />
ERICE (for Evolutionarily Related Interleukin-1 β Converting<br />
Enzyme) and caspase-13 (1). Caspase-13 belongs to the<br />
ICE subfamily of caspases. Overexpression of caspase-13<br />
induces apoptosis. Caspase-13 was activated by caspase-8,<br />
which is a key enzyme in death receptor induced apoptosis.<br />
Caspase-13 is expressed in a variety of human tissues and<br />
cell lines (1).<br />
SOURCE:<br />
Rabbit anti-caspase-13 polyclonal antibody was raised<br />
against a peptide corresponding to amino acids 91 to 105 of<br />
human Acaspase-13 (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of AIF by<br />
Western blot at 0.5 to 1 µg/ml. HL60 cell lysate can be used<br />
as positive control and a 43 kDa band should be detected. It<br />
is human, mouse and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2245P). HL60 cell lysate,<br />
200 µg at 2 mg/ml, is available for positive control (<strong>Catalog</strong><br />
No. 1209).<br />
Western blot analysis of casp-<br />
13 in human HL60 cell lysate<br />
(A), mouse brain (B) and rat<br />
brain (C) tissue lysates with<br />
anti-casp-13 at 1 µg/ml.<br />
REFERENCES:<br />
1. Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable<br />
caspase. J Biol Chem 1998;273:15702-7<br />
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techsupport@prosci-inc.com<br />
Anti-BACE2 / Asp1 (NT)<br />
CATALOG No.: 2247<br />
BACKGROUND:<br />
Accumulation of the amyloid-β (Aβ) plaque in the cerebral<br />
cortex is a critical event in the pathogenesis of Alzheimer’s<br />
disease. Aβ peptide is generated by proteolytic cleavage of<br />
the β−amyloid protein precursor (APP) at β- and γ-sites by<br />
proteases. The long-sought β−secretase was recently<br />
identified by several groups independently and designated<br />
beta-site APP cleaving enzyme (BACE) and aspartyl<br />
protease 2 (Asp2) (1-4). A BACE homolog was recently<br />
cloned and designated BACE2, Asp1, DRAP (for Down<br />
region aspartic protease), and memapsin 1 (4-9). BACE2<br />
also cleaves APP at β-site and at a different site within Aβ<br />
(8). BACE2 locates on chromosome 21q22.3, the so-called<br />
‘Down critical region’, suggesting that BACE2 and Aβ may<br />
also contribute to the pathogenesis of Down syndrome (6,7)<br />
SOURCE:<br />
This rabbit polyclonal antibody was raised against a<br />
synthetic peptide (APTPGPGTPAERHADG) corresponding<br />
to amino acids 44 to 59 of human BACE2 (4).<br />
APPLICATION:<br />
This antibody can be used for detection of BACE2 by<br />
Western blot at 0.5 to 1 µg/ml. Human heart tissue lysate<br />
can be used as positive control. It is human, mouse, and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year. Azide free antibody is<br />
available.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2247P).<br />
Human heart tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1301).<br />
Western blot analysis of<br />
BACE2 in human (A) and<br />
mouse (B) heart tissue<br />
lysates with anti-BACE2<br />
(CT) at 1 µg/ml.<br />
REFERENCES:<br />
1. Vassar R, et al. Beta-secretase cleavage of Alzheimer's amyloid<br />
precursor protein by the transmembrane aspartic protease BACE.<br />
Science 1999;286:735-41<br />
2. Hussain I, et al. Identification of a novel aspartic protease (Asp<br />
2) as beta-secretase. Mol Cell Neurosci 1999;14:419-27<br />
3. Sinha S, et al. Purification and cloning of amyloid precursor<br />
protein beta-secretase from human brain. Nature 1999;402:537-40<br />
4. Yan R, et al. Membrane-anchored aspartyl protease with<br />
Alzheimer's disease beta-secretase activity. Nature 1999;402:533-<br />
7<br />
5. Lin X, et al. Human aspartic protease memapsin 2 cleaves the<br />
beta-secretase site of beta-amyloid precursor protein. Proc Natl<br />
Acad Sci USA 2000 15;97:1456-60<br />
6. Acquati F, et al. The gene encoding DRAP (BACE2), a<br />
glycosylated transmembrane protein of the aspartic protease<br />
family, maps to the down critical region. FEBS Lett 2000;468:59-64<br />
7. Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is<br />
highly similar to Alzheimer's amyloid precursor protein betasecretase.<br />
Cytogenet Cell Genet 2000;89:177-184<br />
8. Farzan M, et al. BACE2, a beta -secretase homolog, cleaves at<br />
the beta site and within the amyloid-beta region of the amyloid-beta<br />
precursor protein. Proc Natl Acad Sci USA 2000;97:9712-7<br />
9. Bennett BD, et al. Expression analysis of BACE2 in brain and<br />
peripheral tissues. J Biol Chem 2000;275:20647-51<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-BACE2 / Asp1 (CT)<br />
CATALOG No.: 2249<br />
BACKGROUND:<br />
Accumulation of the amyloid-β (Aβ) plaque in the cerebral<br />
cortex is a critical event in the pathogenesis of Alzheimer’s<br />
disease. Aβ peptide is generated by proteolytic cleavage of<br />
the β−amyloid protein precursor (APP) at β- and γ-sites by<br />
proteases. The long-sought β−secretase was recently<br />
identified by several groups independently and designated<br />
beta-site APP cleaving enzyme (BACE) and aspartyl<br />
protease 2 (Asp2) (1-4). BACE/Asp2 is a novel<br />
transmembrane aspartic protease and co-localizes with<br />
APP. A BACE homolog was recently cloned and designated<br />
BACE2, Asp1, DRAP (for Down region aspartic protease),<br />
and memapsin 1 (4-9). BACE2 also cleaves APP at β-site<br />
and at a different site within Aβ (8). BACE2 locates on<br />
chromosome 21q22.3, the so-called ‘Down critical region’,<br />
suggesting that BACE2 and Aβ may also contribute to the<br />
pathogenesis of Down syndrome (6,7)<br />
SOURCE:<br />
This rabbit polyclonal antibody was raised against a peptide<br />
corresponding to amino acids 496 to 511 of human BACE2<br />
(4).<br />
APPLICATION:<br />
This antibody can be used for detection of BACE2 by<br />
Western blot at 0.5 to 1 µg/ml. Human heart tissue lysate<br />
can be used as positive control. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year. Azide free antibody is<br />
available.<br />
RELATED PRODUCTS:<br />
Western blot analysis of<br />
BACE2 in human heart<br />
tissue lysate in the absence<br />
(A) or presence (B) of<br />
blocking peptide (2249P)<br />
with anti-BACE2 (CT) at 1<br />
µg/ml.<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2249P).<br />
Human heart tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1301).<br />
REFERENCES:<br />
1. Vassar R, et al. Beta-secretase cleavage of Alzheimer's amyloid<br />
precursor protein by the transmembrane aspartic protease BACE.<br />
Science 1999;286:735-41<br />
2. Hussain I, et al. Identification of a novel aspartic protease (Asp<br />
2) as beta-secretase. Mol Cell Neurosci 1999;14:419-27<br />
3. Sinha S, et al. Purification and cloning of amyloid precursor<br />
protein beta-secretase from human brain. Nature 1999;402:537-40<br />
4. Yan R, et al. Membrane-anchored aspartyl protease with<br />
Alzheimer's disease beta-secretase activity. Nature 1999;402:533-<br />
7<br />
5. Lin X, et al. Human aspartic protease memapsin 2 cleaves the<br />
beta-secretase site of beta-amyloid precursor protein. Proc Natl<br />
Acad Sci USA 2000 15;97:1456-60<br />
6. Acquati F, et al. The gene encoding DRAP (BACE2), a<br />
glycosylated transmembrane protein of the aspartic protease<br />
family, maps to the down critical region. FEBS Lett 2000;468:59-64<br />
7. Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is<br />
highly similar to Alzheimer's amyloid precursor protein betasecretase.<br />
Cytogenet Cell Genet 2000;89:177-184<br />
8. Farzan M, et al. BACE2, a beta-secretase homolog, cleaves at<br />
the beta site and within the amyloid-beta region of the amyloid-beta<br />
precursor protein. Proc Natl Acad Sci USA 2000;97:9712-7<br />
9. Bennett BD, et al. Expression analysis of BACE2 in brain and<br />
peripheral tissues. J Biol Chem 2000;275:20647-51<br />
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Anti-BACE / Asp2 (CT)<br />
CATALOG No.: 2253<br />
BACKGROUND:<br />
Accumulation of the amyloid-β (Aβ) plaque in the cerebral<br />
cortex is a critical event in the pathogenesis of Alzheimer’s<br />
disease. Aβ peptide is generated by proteolytic cleavage of<br />
the β−amyloid protein precursor (APP) at β- and γ-sites by<br />
two proteases. APP is first cleaved by β−secretase,<br />
producing a soluble derivative of the protein and a<br />
membrane anchored 99-amino acid carboxy-terminal<br />
fragment (C99). The C99 fragment serves as substrate for<br />
γ−secretase to generate the 4 kDa amyloid-β peptide, which<br />
is deposited in the brains of all suffers of Alzheimer’s<br />
disease. The long-sought β−secretase was recently<br />
identified by several groups independently and designated<br />
beta-site APP cleaving enzyme (BACE) and aspartyl<br />
protease 2 (Asp2) (1-4). BACE/Asp2 is a novel<br />
transmembrane aspartic protease and colocalizes with APP.<br />
SOURCE:<br />
This rabbit polyclonal antibody was raised against a peptide<br />
corresponding to amino acids 485 to 501 of human BACE<br />
(1). The peptide sequence differs from those of mouse and<br />
rat BACE by one amino acid (1).<br />
APPLICATION:<br />
This antibody can be used for detection of BACE by Western<br />
blot at 0.5 to 1 µg/ml. Human brain tissue lysate can be used<br />
as positive control and an approximately 70 kDa band<br />
should be detected. It is human and mouse reactive. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year. Azide free antibody is<br />
available.<br />
Western blot analysis of<br />
BACE in human brain tissue<br />
lysate in the absence (A) or<br />
presence (B) of blocking<br />
peptide (2253P) and in mouse<br />
3T3 cell lysate (C) with anti-<br />
BACE (CT) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2253P).<br />
Human brain tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1303).<br />
REFERENCES:<br />
1. Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase<br />
cleavage of Alzheimer's amyloid precursor protein by the<br />
transmembrane aspartic protease BACE. Science 1999;286:735-<br />
41<br />
2. Hussain I, Powell D, Howlett DR, et al. Identification of a novel<br />
aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci<br />
1999;14:419-27<br />
3. Yan R, Bienkowski MJ, Shuck ME, et al. Membrane-anchored<br />
aspartyl protease with Alzheimer's disease beta-secretase activity.<br />
Nature 1999;402:533-7<br />
4. Sinha S, Anderson JP, Barbour R, et al. Purification and cloning<br />
of amyloid precursor protein beta-secretase from human brain.<br />
Nature 1999;402:537-40 (WD0500)
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Fax: 858-513-2692<br />
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Anti-AIF (IN)<br />
CATALOG No.: 2267<br />
BACKGROUND:<br />
Apoptosis is characterized by several morphological nuclear<br />
changes including chromatin condensation and nuclear<br />
fragmentation. These changes are triggered by the activation<br />
of members of caspase family, caspase activated DNase,<br />
and several novel proteins (1). A novel gene, the product of<br />
which causes chromatin condensation and DNA<br />
fragmentation, was recently identified, cloned, and<br />
designated apoptosis inducing factor (AIF) (2). Like the<br />
critical molecules, cytochrome c and caspase-9, in<br />
apoptosis, AIF localizes in mitochondria. AIF translocates to<br />
the nucleus when apoptosis is induced and induces<br />
mitochondria to release the apoptogenic proteins<br />
cytochrome c and caspase-9. AIF induces chromatin<br />
condensation and large scale DNA fragmentation, which are<br />
the hallmarks of apoptosis, of the isolated nucleus and the<br />
nucleus in live cells by microinjection and apoptosis stimuli<br />
(2,3). AIF is highly conserved between human and mouse<br />
and widely expressed (2).<br />
SOURCE:<br />
Rabbit anti-AIF (IN) polyclonal antibody was raised against a<br />
peptide corresponding to amino acids 517 to 531 of human<br />
AIF (2). This sequence is identical to those of mouse and rat<br />
AIF (2).<br />
Western blot analysis of AIF<br />
in K562 cell lysate (A), rat<br />
heart (B), and mouse heart<br />
(C) tissue lysates with anti-<br />
AIF (IN) at 1 µg/ml.<br />
STORAGE:<br />
It is supplied as ion exchange chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2267P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature<br />
1999;401:127-8<br />
2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular<br />
characterization of mitochondrial apoptosis-inducing factor. Nature<br />
1999;397:441-6<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of AIF by<br />
Western blot at 0.25 to 1 µg/ml. K562 cell lysate can be used<br />
as positive control and a 67 kDa band should be detected. It<br />
is human, mouse and rat reactive. For research use only.<br />
3. Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear<br />
translocation of AIF in apoptosis and necrosis. FASEB J<br />
2000;14:729-39<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-FLASH (CT)<br />
CATALOG No.: 2271<br />
BACKGROUND:<br />
A novel mammalian CED-4 homologous was recently<br />
identified and cloned in mouse (1) and human (2) and<br />
designated FLASH (for FLICE-associated huge protein).<br />
FLASH is involved in Fas induced apoptosis. It is recruited to<br />
Fas after the receptor cross-linking. Overexpression of wild<br />
type of FLASH facilitates and its dominant negative form<br />
inhibits Fas induced apoptosis. FLASH interacts with the<br />
DEDs of caspase-8 and FADD through the DED-like domain<br />
of FLASH and mediates activation of caspase-8 (1). There<br />
are parallels between FLASH and Apaf-1/CED-4 although<br />
there are arguments against their structural similarity (1,2).<br />
FLASH is widely expressed.<br />
SOURCE:<br />
Rabbit anti-FLASH (CT) polyclonal antibody was raised<br />
against a synthetic peptide (SERFQQLMKLFEKSKC)<br />
corresponding to amino acids 1253 to 1268 of human origin,<br />
which differ from those of mouse by one amino acid (1,2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of FLASH<br />
by Western blot at 0.25 to 0.5 µg/ml. HeLa cell lysate can be<br />
used as positive control and an approximate 220 kDa band<br />
can be detected. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
FLASH in HeLa whole cell<br />
lysate with anti-FLASH (CT)<br />
at 0.5 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2271P).<br />
HeLa cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1201).<br />
REFERENCES:<br />
1. Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K,<br />
Yonehara S. The CED-4-homologous protein FLASH is involved in<br />
Fas-mediated activation of caspase-8 during apoptosis. Nature<br />
1999;398:777-85<br />
2. Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM<br />
Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3<br />
3. Medema JP. Apoptosis. Life and death in a FLASH. Nature<br />
1999;398:756-7<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-F1Aα<br />
CATALOG No.: 2279<br />
BACKGROUND:<br />
Fas and tumor necrosis factor receptor 1 (TNFR1) are two<br />
prototype members in the death receptor family. A novel<br />
protein that associates with the intracellular domains of Fas<br />
and TNFR1 was recently identified and designated<br />
F1Aα and FEM1β (1,2). F1Aα/FEM1β is the homologue of C.<br />
elegans sex determining protein FEM-1 (1,2). FEM-<br />
1/F1Aα is cleaved by CED-3 and caspase (3). FEM-<br />
1/F1Aα associates with CED-4 and its mammalian<br />
homologue Apaf-1 (3). Overexpression of F1Aα induces<br />
apoptosis. F1Aα is therefore a novel member of the death<br />
receptor associated protein that mediates apoptosis. F1Aα<br />
is expressed in a variety of human and mouse tissues (1,2)<br />
SOURCE:<br />
Rabbit anti-F1Aα polyclonal antibody was raised against a<br />
peptide (DINYQDQIPRTLEE) corresponding to amino acids<br />
609 to 622 of human F1Aα (1). This sequence is identical to<br />
the corresponding sequence of mouse FEM1β (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of F1Aα<br />
by Western blot at 0.5 to 1 µg/ml. Mouse liver tissue lysate<br />
can be used as positive control and an approximately 70 kDa<br />
band can be detected. It is human, mouse, and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2279P).<br />
Mouse liver tissue lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1404).<br />
Western blot analysis of F1Aα<br />
in mouse (A) and rat (B) liver<br />
tissue lysates with anti- F1Aα<br />
at 1 µg /ml.<br />
REFERENCES:<br />
1. Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu<br />
VC. F1Aalpha, a death receptor-binding protein homologous to the<br />
Caenorhabditis elegans sex-determining protein, FEM-1, is a<br />
caspase substrate that mediates apoptosis. J Biol Chem.<br />
1999;274(45):32461-8.<br />
2. Ventura-Holman T, Maher JF. Sequence, organization, and<br />
expression of the human FEM1B gene. Biochem Biophys Res<br />
Commun 2000;267(1):317-20<br />
3. Chan SL, Yee KS, Tan KM, Yu VC. The Caenorhabditis elegans<br />
sex determination protein FEM-1 is a CED-3 substrate that<br />
associates with CED-4 and mediates apoptosis in mammalian<br />
cells. J Biol Chem. 2000;275(24):17925-8.<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-RIP3<br />
CATALOG No.: 2283<br />
BACKGROUND:<br />
Certain serine/threonine protein kinases, such as ASK1, RIP,<br />
DAP, and ZIP kinases, are mediators of apoptosis. Receptor<br />
interacting proteins including RIP and RIP2/RICK mediate<br />
apoptosis induced by TNFR1 and Fas, two prototype<br />
members in the death receptor family. A novel member in<br />
the RIP kinase family was recently identified and designated<br />
RIP3 (1-3). RIP3 contains N-terminal kinase domain but,<br />
unlike RIP or RIP2, lacks the C-terminal death or CARD<br />
domain. RIP3 binds to RIP and TNFR1, mediates TNFR1<br />
induced apoptosis, and attenuates RIP and TNFR1 induced<br />
NF-κB activation. Overexpression of RIP3 induces apoptosis<br />
and NF-κB activation. The messenger RNA of RIP3 is<br />
expressed in a subset of adult tissues (2,3).<br />
SOURCE:<br />
Rabbit anti-RIP3 polyclonal antibody was raised against a<br />
peptide (AQFGRGRGWQPFHK) corresponding to amino<br />
acids 473 to 486 of murine RIP3 (3).<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2383P).<br />
Mouse 3T3 whole cell lysate, 200 µg at 2 mg/ml, is<br />
available for positive control (<strong>Catalog</strong> No. 1212).<br />
Western blot analysis of RIP3 in<br />
mouse 3T3 whole cell lysate in<br />
the absence (A) or presence (B)<br />
of blocking peptide with anti-<br />
RIP3 at 1 µg /ml.<br />
REFERENCES:<br />
1. Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP,<br />
Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that<br />
activates apoptosis and NFkappaB. Curr Biol. 1999;9(10):539-42.<br />
2. Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3,<br />
a novel apoptosis-inducing kinase. J Biol Chem.<br />
1999;274(24):16871-5.<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of RIP3<br />
by Western blot at 0.5 to 1 µg/ml. 3T3 cell lysate can be<br />
used as positive control and an approximately 57 kDa band<br />
can be detected. It is mouse and rat reactive. For research<br />
use only.<br />
3. Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse<br />
receptor interacting protein 3 does not contain a caspase-recruiting<br />
or a death domain but induces apoptosis and activates NFkappaB.<br />
Mol Cell Bio. 1999; 19(10):6500-8<br />
(WD0102)<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-ASC / TMS1<br />
CATALOG No.: 2287<br />
BACKGROUND:<br />
Apoptosis is regulated by death domain (DD) and/or caspase<br />
recruitment domain (CARD) containing molecules and a<br />
caspase family of proteases. CARD containing cell death<br />
regulators include RAIDD, RICK, BCL10, Apaf-1, ARC,<br />
caspase-9, and caspase-2. A novel CARD domain<br />
containing protein was recently identified in human and<br />
mouse and designated ASC and TMS1 (1-3). Ectopic<br />
expression of ASC/TMS1 induced apoptosis through<br />
activation of caspase-9 and inhibited the survival of human<br />
breast cancer cells (3,4). Overexpression of ASC/TMS1<br />
induced DNA fragmentation (4). ASC/TMS1 is expressed in<br />
a variety of human and mouse tissues (1, 2)<br />
SOURCE:<br />
Rabbit anti-ASC/TMS1 polyclonal antibody was raised<br />
against a synthetic peptide (RESQSYLVEDLERS)<br />
corresponding to amino acids 182 to 195 of human ASC (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
ASC/TMS1 by Western blot at 1 µg/ml. HL60 whole cell<br />
lysate can be used as positive control and an approximately<br />
25 kDa band can be detected. It is human reactive. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2287P). HL60 whole cell<br />
lysate, 200 µg at 2 mg/ml, is available for positive control<br />
(<strong>Catalog</strong> No. 1209).<br />
Western blot analysis of ASC in<br />
HL60 whole cell lysate in the<br />
absence (A) or presence (B) of<br />
blocking peptide (2287P) with anti-<br />
ASC at 1 µg /ml.<br />
REFERENCES:<br />
1. Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T,<br />
Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a<br />
novel 22-kDa protein, aggregates during apoptosis of human<br />
promyelocytic leukemia HL-60 cells. J Biol Chem.<br />
1999;274(48):33835-8.<br />
2. Masumoto J, Taniguchi S, Nakayama K, Ayukawa K, Sagara J.<br />
Murine Ortholog of ASC, a CARD-Containing Protein, Self-<br />
Associates and Exhibits Restricted Distribution in Developing<br />
Mouse Embryos. Exp Cell Res. 2001;262(2):128-133.<br />
3. Conway KE, McConnell BB, Bowring CE, Donald CD, Warren<br />
ST, Vertino PM. TMS1, a novel proapoptotic caspase recruitment<br />
domain protein, is a target of methylation-induced gene silencing in<br />
human breast cancers. Cancer Res. 2000;60(22):6236-42.<br />
4. McConnell BB, Vertino PM. Activation of a caspase-9-mediated<br />
apoptotic pathway by subcellular redistribution of the novel<br />
caspase recruitment domain protein TMS1. Cancer Res.<br />
2000;60(22):6243-7.<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Bnip3L (IN) Bnip3α, Nix<br />
CATALOG No.: 2289<br />
BACKGROUND:<br />
Members in the Bcl-2 family are critical regulators of<br />
apoptosis by either inhibiting or promoting cell death. Bcl-2<br />
homology 3 (BH3) domain is a potent death domain. BH3<br />
domain containing pro-apoptotic proteins, including Bad,<br />
Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass<br />
of the Bcl-2 family. A novel BH3 domain containing protein<br />
was recently identified and designated Bnip3L, Bnip3α, and<br />
Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3α/Nix is a<br />
homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic<br />
protein Bnip3. Overexpression of Bnip3L induces apoptosis<br />
(2,3). Bnip3L interacts with and overcomes suppresses by<br />
Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The<br />
messenger RNA of Bnip3L is ubiquitously expressed in<br />
human tissues (1,2). Bnip3L and Bnip3 form a new subfamily<br />
of the pro-apoptotic mitochondrial proteins.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of Bnip3L<br />
in K562 whole cell lysate in the<br />
absence (A), or presence (B) of<br />
immunogenic peptide (2289P)<br />
with anti-Bnip3L (IN) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2289P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
SOURCE:<br />
Rabbit anti-Bnip3L (IN) polyclonal antibody was raised<br />
against a peptide (DAQHESGQSSSRGSSH) corresponding<br />
to amino acids 77 to 92 of human origin, which are identical<br />
to those of mouse Bnip3L (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of Bnip3L<br />
by Western blot at 0.5 to 1 µg/ml. K562 cell lysate can be<br />
used as positive control and the approximate 40 kDa top<br />
band represents monomer of Bnip3L (4). It is human and<br />
mouse reactive. For research use only.<br />
REFERENCES:<br />
1. Matsushima M, Fujiwara T, Takahashi E, et al. Isolation,<br />
mapping, and functional analysis of a novel human cDNA (BNIP3L)<br />
encoding a protein homologous to human NIP3. Genes<br />
Chromosomes Cancer 1998;21:230-5<br />
2. Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G.<br />
BNIP3alpha: a human homolog of mitochondrial proapoptotic<br />
protein BNIP3. Cancer Res 1999;59:533-7<br />
3. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a<br />
subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem<br />
1999;274(1):7-10<br />
4. Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDainteracting<br />
protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL<br />
and induces apoptosis by altering mitochondrial membrane<br />
permeability. Oncogene. 1999;18:4523-9 (RD1200)
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Anti-DcR1 (ED2) TRAIL-R3, TRID, LIT<br />
CATALOG No.: 2299<br />
BACKGROUND:<br />
Apoptosis is induced by certain cytokines including TNF and<br />
Fas ligand in the TNF family through their death domain<br />
containing receptors. TRAIL/Apo2L is a new member of the<br />
TNF family and induces apoptosis of a variety of tumor cell<br />
lines. DR4 and DR5 are the recently identified functional<br />
receptors for TRAIL (1-3). Two decoy receptors for TRAIL<br />
have been identified and designated DcR1/TRID/TRAIL-<br />
R3/LIT (2-7) and DcR2/TRAIL-R4/TRUNDD (8-10). DcR1<br />
has extracellular TRAIL-binding domain but lacks<br />
intracellular signaling domain. It is a glycophospholipidanchored<br />
cell surface protein. DcR1 transcripts are<br />
expressed in many normal human tissues but not in most<br />
cancer cell lines (2,3). Overexpression of DcR1 did not<br />
induce apoptosis, but attenuated TRAIL-induced apoptosis<br />
(2,3).<br />
SOURCE:<br />
Rabbit anti-DcR1 (ED2) polyclonal antibody was raised<br />
against a peptide (CKEGTFRNENSPE) corresponding to<br />
amino acids 111 to 123 at extracellular domain of human<br />
DcR1 precursor (2-3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DcR1<br />
by Western blot at 0.5 to 1 µg/ml. HeLa cell lysate can be<br />
used as positive control and an approximate 65 kDa band<br />
can be detected (6). It is human, mouse, and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as chromatography purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at -20°C.<br />
Stable for one year at 2-8°C.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2299P).<br />
HeLa cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1201).<br />
Western blot analysis of DcR1 in<br />
HeLa cell (A), mouse (B) and rat (C)<br />
liver tissue lysates with anti-DcR1<br />
(ED2) at 1 µg/ml.<br />
REFERENCES:<br />
1. Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic<br />
ligand TRAIL. Science; 1997;276:111-113<br />
2. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death<br />
domain-containing receptor for TRAIL. Science 1997;277:815-8<br />
3. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced<br />
apoptosis by a family of signaling and decoy receptors. Science<br />
1997;277:818-21<br />
4. Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and<br />
characterization of TRAIL-R3, a novel member of the emerging TRAIL<br />
receptor family. J Exp Med 1997;186(7):1165-70<br />
5. MacFarlane M, Ahmad M, Srinivasula SM, et al. Identification and<br />
molecular cloning of two novel receptors for the cytotoxic ligand TRAIL. J<br />
Biol Chem 1997;272(41):25417-20<br />
6. Schneider P, Bodmer JL, Thome M, Hofmann K, Holler N, Tschopp J.<br />
Characterization of two receptors for TRAIL. FEBS Lett 1997;416(3):329-34<br />
7. Mongkolsapaya J, Cowper AE, Xu XN, et al. Lymphocyte inhibitor of<br />
TRAIL (TNF-related apoptosis -inducing ligand): a new receptor protecting<br />
lymphocytes from the death ligand TRAIL. J Immunol 1998;160(1):3-6<br />
8. Marsters SA, Sheridan JP, Pitti RM, et al. A novel receptor for<br />
Apo2L/TRAIL contains a truncated death domain. Curr Biol 1997;7:1003-6<br />
9. Degli-Esposti MA, Dougall WC, Smolak PJ, et al. The novel receptor<br />
TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated<br />
apoptosis, yet retains an incomplete death domain. Immunity 1997;7:813-20<br />
10. Pan G, Ni J, Yu G, et al. TRUNDD, a new member of the TRAIL<br />
receptor family that antagonizes TRAIL signalling. FEBS Lett 1998;424:41-5<br />
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Anti-AIF (CT)<br />
CATALOG No.: 2301<br />
BACKGROUND:<br />
Apoptosis is characterized by several morphological nuclear<br />
changes including chromatin condensation and nuclear<br />
fragmentation. These changes are triggered by the activation<br />
of members of caspase family, caspase activated DNase,<br />
and several novel proteins (1). A novel gene, the product of<br />
which causes chromatin condensation and DNA<br />
fragmentation, was recently identified, cloned, and<br />
designated apoptosis inducing factor (AIF) (2). Like the<br />
critical molecules, cytochrome c and caspase-9, in<br />
apoptosis, AIF localizes in mitochondria. AIF translocates to<br />
the nucleus when apoptosis is induced and induces<br />
mitochondria to release the apoptogenic proteins<br />
cytochrome c and caspase-9. AIF induces chromatin<br />
condensation and DNA fragmentation, which are the<br />
hallmarks of apoptosis, of the isolated nucleus and the<br />
nucleus in live cells by microinjection. AIF is highly<br />
conserved between human and mouse and widely<br />
expressed (2).<br />
SOURCE:<br />
Rabbit anti-AIF (CT) polyclonal antibody was raised against<br />
a peptide (KDGEQHEDLNEVAK) corresponding to amino<br />
acids 593 to 606 of human AIF (2). This sequence is<br />
identical to those of mouse and rat AIF (2).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of AIF in<br />
K562 cell lysate (A), mouse (B),<br />
and rat (C) liver tissue lysates<br />
with anti-AIF (CT) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2301P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
REFERENCES:<br />
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature<br />
1999;401:127-8<br />
2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular<br />
characterization of mitochondrial apoptosis-inducing factor. Nature<br />
1999;397:441-6<br />
(WD0800)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of AIF by<br />
Western blot at 0.25 to 1 µg/ml. K562 cell lysate can be used<br />
as positive control and a 67 kDa band should be detected. It<br />
is human, mouse, and rat reactive. For research use only.
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Anti-DAP Kinase 2<br />
CATALOG No.: 2323<br />
BACKGROUND:<br />
Apoptosis is mediated by death domain containing adapter<br />
molecules and a caspase family of proteases. Certain<br />
serine/threonine protein kinases, such as RIP and DAP<br />
kinase, are mediators of apoptosis. DAP kinase (DAPK) is<br />
pro-apoptotic calcium-regulated serine/threonine kinase<br />
containing death domain. Ectopic expression of DAPK<br />
induces cell death and suppresses oncogenic<br />
transformation. DAPK mediates IFNγ induced apoptosis. A<br />
novel DAP kinase-related protein was recently identified and<br />
designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed<br />
DAPK2 induced apoptosis in various types of cells (1,2).<br />
DAPK has high sequence homology to ZIP kinase and<br />
DRAK1/2, and they represent a novel family of<br />
serine/threonine kinases, which mediates apoptosis through<br />
their catalytic activities. The messenger RNA of DAPK2 is<br />
expressed in multiple human tissues (1).<br />
SOURCE:<br />
Rabbit anti-DAPK2 polyclonal antibody was raised against a<br />
peptide (ARRKALHPRRRSSTS) corresponding to amino<br />
acids 356 to 370 of human DAPK2 (1,2). The sequence of<br />
this antigenic peptide is identical to the corresponding amino<br />
acids of mouse origin (1,2)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of DAPK2<br />
by Western blot at 0.5 to 1 µg/ml. A431 whole cell lysate can<br />
be used as positive control and an approximately 42 kDa<br />
band can be detected. It is human, mouse, and rat reactive,<br />
and has no cross responses to DAPK1. For research use<br />
only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
DAPK2 in A431 (H), mouse<br />
spleen (M), and rat kidney (R)<br />
lysates with anti-DAPK2<br />
(2323) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2323P).<br />
A431 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1202).<br />
REFERENCES:<br />
1. Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ,<br />
Jenkins NA, Akira S. Death-associated protein kinase 2 is a new<br />
calcium/calmodulin-dependent protein kinase that signals<br />
apoptosis through its catalytic activity. Oncogene<br />
1999;18(23):3471-80<br />
2. Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Deathassociated<br />
protein kinase-related protein 1, a novel<br />
serine/threonine kinase involved in apoptosis. Mol Cell Biol<br />
2000;20(3):1044-54<br />
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techsupport@prosci-inc.com<br />
Anti-Caspase-12 (NT)<br />
CATALOG No.: 2325<br />
BACKGROUND:<br />
Three distinct signaling pathways lead to programmed cell<br />
death (apoptosis). The death receptor and mitochondrion<br />
pathways are the mains, in which the key apoptotic<br />
proteases capase-8 and caspase-9, respectively, are<br />
involved. The endoplasmic reticulum (ER) stress is the third<br />
apoptotic pathway and caspase-12 is involved (1,2).<br />
Caspase-12 is localized to the ER but not to cytoplasm or<br />
mitochondrion. Caspase-12 is activated by ER stress,<br />
including disruption of ER calcium homeostasis, and<br />
mediates ER stress-induced apoptosis. Caspase-12 is colocalized<br />
to the ER with several proteins that are involved in<br />
Alzheimer’s disease including γ-secretase presenilin and β-<br />
amyloid precursor protein (APP). Caspase-12 mediates<br />
cytotoxicity induced by amyloid-β. Caspase-12 is<br />
ubiquitously expressed in mouse tissues (1).<br />
SOURCE:<br />
Rabbit anti-caspase-12 (NT) polyclonal antibody was raised<br />
against a peptide (AARRTHERDPIYKIKG) corresponding<br />
to amino acids 2 to 17 of murine caspase-12 (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
caspase-12 by Western blot at 0.5 to 1 µg/ml. Murine spleen<br />
tissue lysate can be used as positive control. It is human,<br />
mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
caspase-12 in human (A)<br />
and mouse (B) spleen<br />
tissue lysates with anticaspase-12<br />
(NT) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2325P).<br />
Murine spleen tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1406).<br />
REFERENCES:<br />
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA,<br />
Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific<br />
apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-<br />
103.<br />
2. Mehmet H. Caspases find a new place to hide. Nature<br />
2000;403:29-30<br />
3. Van de Craen M, Vandenabeele P, Declercq W, Van den<br />
Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W,<br />
Beyaert R, Fiers W. Characterization of seven murine caspase<br />
family members. FEBS Lett 1997;403:61-9<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
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Anti-Caspase-12 (IN)<br />
CATALOG No.: 2327<br />
BACKGROUND:<br />
Three distinct signaling pathways lead to programmed cell<br />
death (apoptosis). The death receptor and mitochondrion<br />
pathways are the mains, in which the key apoptotic<br />
proteases capase-8 and caspase-9, respectively, are<br />
involved. The endoplasmic reticulum (ER) stress is the third<br />
apoptotic pathway and caspase-12 is involved (1,2).<br />
Caspase-12 is localized to the ER but not to cytoplasm or<br />
mitochondrion. Caspase-12 is activated by ER stress,<br />
including disruption of ER calcium homeostasis, and<br />
mediates ER stress-induced apoptosis. Caspase-12 is colocalized<br />
to the ER with several proteins that are involved in<br />
Alzheimer’s disease including γ-secretase presenilin and β-<br />
amyloid precursor protein (APP). Caspase-12 mediates<br />
cytotoxicity induced by amyloid-β. Caspase-12 is<br />
ubiquitously expressed in mouse tissues (1).<br />
SOURCE:<br />
Rabbit anti-caspase-12 (IN) polyclonal antibody was raised<br />
against a peptide (CTPSSPSESKRKVEDDE) corresponding<br />
to amino acids 100 to 116 of murine caspase-12 (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
caspase-12 by Western blot at 0.5 to 1 µg/ml. Murine brain<br />
tissue lysate can be used as positive control. It is human,<br />
mouse, and rat reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
caspase-12 in murine brain<br />
tissue lysate in the absence<br />
(A) or presence (B) of<br />
blocking peptide with anticaspase-12<br />
(IN) at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2327P).<br />
Murine brain tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1403).<br />
REFERENCES:<br />
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA,<br />
Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific<br />
apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-<br />
103.<br />
2. Mehmet H. Caspases find a new place to hide. Nature<br />
2000;403:29-30<br />
3. Van de Craen M, Vandenabeele P, Declercq W, Van den<br />
Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W,<br />
Beyaert R, Fiers W. Characterization of seven murine caspase<br />
family members. FEBS Lett 1997;403:61-9<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IKKε / IKK-i (CT)<br />
CATALOG No.: 2329<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli. NF-κB is associated with<br />
IκB proteins in the cell cytoplasm, which inhibit NF-κB<br />
activity. IκB is phosphorylated by IκB kinase (IKK) complex<br />
that contains IKKα, IKKβ, and IKKγ. A novel molecule in the<br />
IKK complex was recently identified and designated<br />
IKKε/IKK-i (1,2). IKKε is required for the activation of NF-κB<br />
by PMA and T cell receptors but not by TNFα and IL-1 (1).<br />
IKKε/IKK-i message is expressed in a variety of tissues and<br />
is inducible by TNFα, IL-1, and LPS (2).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
IKKε in Jurkat whole cell<br />
lysate with anti-IKKε/IKK-i<br />
at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2329P).<br />
SOURCE:<br />
Rabbit anti-IKKε polyclonal antibody was raised against a<br />
peptide (NRIIERLNRVPAPPDV) corresponding to amino<br />
acids 701 to 716 of human IKKε/IKK-i (1,2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IKKε by<br />
Western blot at 0.5 to 1 µg/ml. Jurkat whole cell lysate can<br />
be used as positive control and an 80 kDa band should be<br />
detected. It has no cross response to IKKα, IKKβ, or IKKγ.<br />
For research use only.<br />
REFERENCES:<br />
1. Peters RT, Liao SM, Maniatis T. IKKepsilon is part of a novel<br />
PMA-inducible IkappaB kinase complex. Mol Cell 2000;5(3):513-22<br />
2. Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi<br />
Y, Kanamaru A, Akira S. IKK-i, a novel lipopolysaccharideinducible<br />
kinase that is related to IkappaB kinases. Int Immunol<br />
1999;11(8):1357-62<br />
(WD0101)
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Poway, CA 92064<br />
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Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
Anti-IKKγ / NEMO (NT)<br />
CATALOG No.: 2335<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli. NF-κB is associated with<br />
IκB proteins in the cell cytoplasm, which inhibit NF-κB<br />
activity. The IκB kinase (IKKα and IKKβ) phosphorylates IκB<br />
and mediates NF-κB activation. A novel molecule in the IKK<br />
complex was recently identified and termed<br />
IKKγ/NEMO/FIP3/IKKAP1 (1-5). IKKγ interacts with IKKβ and<br />
is required for the activation of IKK complex and NF-κB by<br />
LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also<br />
shown to bind to RIP and NIK and to mediate TNF-induced<br />
NF-κB activation (3).<br />
SOURCE:<br />
Rabbit anti-IKKγ polyclonal antibody was raised against a<br />
peptide (CQYQAPDMDTLQIHVME) corresponding to amino<br />
acids 400 to 416 of human IKKγ (1,2), which are identical to<br />
those of mouse homologue (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IKKγ by<br />
Western blot at 0.5 to 1 µg/ml. HeLa whole cell lysate can be<br />
used as positive control and a 52 kDa band should be<br />
detected. It is human, mouse, and rat reactive and has no<br />
cross response to IKKα or IKKβ. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2335P).<br />
Western blot analysis of IKKγ<br />
in HeLa whole cell lysate with<br />
anti-IKKγ/NEMO at 1 µg/ml.<br />
REFERENCES:<br />
1. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an<br />
essential regulatory subunit of the IkappaB kinase complex. Nature<br />
1998;395(6699):297-300<br />
2. Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou<br />
F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a<br />
component of the IkappaB kinase complex essential for NFkappaB<br />
activation. Cell. 1998;93(7):1231-40.<br />
3. Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A,<br />
Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a<br />
modulator of NF-kappaB activity and as a target of an adenovirus<br />
inhibitor of tumor necrosis factor alpha-induced apoptosis. Proc<br />
Natl Acad Sci U S A 1999;96(3):1042-7<br />
4. Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB,<br />
Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM.<br />
IkappaB kinase (IKK)-associated protein 1, a common component<br />
of the heterogeneous IKK complex. Mol Cell Biol.<br />
1999;19(2):1526-38.<br />
5. Jin DY, Jeang KT. Isolation of full-length cDNA and<br />
chromosomal localization of human NF-kappaB modulator NEMO<br />
to Xq28. J Biomed Sci 1999;6(2):115-20<br />
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Anti-DC-SIGN (CT)<br />
CATALOG No.: 2347<br />
BACKGROUND:<br />
Dendritic cells (DCs) that control immune responses were<br />
recently found to capture and transport HIV from the<br />
mucosal area to remote lymph nodes (1), where DCs hand<br />
over HIV to CD4 + T lymphocytes. DCs also amplify the<br />
amount of virus and extend the duration of viral infectivity.<br />
Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-<br />
SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A<br />
DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-<br />
SIGN2) was identified recently (4-8). DC-SIGN forms a novel<br />
gene family with DC-SIGNR and many alternatively spliced<br />
isoforms of DC-SIGN and DC-SIGNR (8). The expression of<br />
DC-SIGN was found in mucosal tissues including placenta,<br />
small intestine, and rectum.<br />
SOURCE:<br />
Rabbit anti-DC-SIGN polyclonal antibody was raised against<br />
a synthetic peptide (CSRDEEQFLSPAPATPN PPPA)<br />
corresponding to amino acids 384 to 404 of human DC-<br />
DIGN (1).<br />
APPLICATION:<br />
This antibody can be used for detection of DC-SIGN by<br />
Western blot at 1 to 2 µg/ml. Human placenta lysate can be<br />
used as a positive control. A band at approximately 44 kDa<br />
can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2347P).<br />
Human placenta lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1309).<br />
Western blot detection of<br />
GST-DC-SIGN<br />
fusion<br />
protein expressed in E. Coli<br />
in the absence (lane 1) and<br />
presence (lane 2) of<br />
inducing reagent IPTG with<br />
anti-DC-SIGN at 0.5 µg /ml.<br />
REFERENCES:<br />
1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a<br />
dendritic cell-specific HIV -1-binding protein that enhances transinfection<br />
of T cells. Cell. 2000;100:587-97.<br />
2. Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN<br />
interactions with human immunodeficiency virus type 1 and 2 and<br />
simian immunodeficiency virus. J Virol. 2001;75(10):4664-72.<br />
3. Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification<br />
of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that<br />
supports primary immune responses. Cell. 2000;100(5):575-85.<br />
4. Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene,<br />
DC-SIGNR; and CD23 form a cluster on 19p13. J Immunol.<br />
2000;165(6):2937-42.<br />
5. Pohlmann S, Soilleux EJ, Baribaud F, et al DC-SIGNR, a DC-<br />
SIGN homologue expressed in endothelial cells, binds to human<br />
and simian immunodeficiency viruses and activates infection in<br />
trans. Proc Natl Acad Sci USA. 2001;98(5):2670-2675.<br />
6. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, at el. A<br />
dendritic cell-specific intercellular adhesion molecule 3-grabbing<br />
nonintegrin (DC-SIGN)-related protein is highly expressed on<br />
human liver sinusoidal endothelial cells and promotes HIV -1<br />
infection. J Exp Med. 2001;193(6):671-8.<br />
7. Mitchell DA, Fadden AJ, Drickamer K. A novel mechanism of<br />
carbohydrate recognition by the C-type lectins DC-SIGN and DC-<br />
SIGNR: Subunit organisation and binding to multivalent ligands. J<br />
Biol Chem. 2001 in press<br />
8. Mummidi S, Catano G, Lam L, et al. Extensive repertoire of<br />
membrane-bound and soluble DC-SIGN1 and DC-SIGN2 isoforms:<br />
Inter-individual variation in expression of DC-SIGN transcripts. J<br />
Biol Chem. 2001 in press<br />
(WD0108)
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-DC-SIGN (ED)<br />
CATALOG No.: 2349<br />
BACKGROUND:<br />
Dendritic cells (DCs) that control immune responses were<br />
recently found to capture and transport HIV from the<br />
mucosal area to remote lymph nodes (1), where DCs hand<br />
over HIV to CD4 + T lymphocytes. DCs also amplify the<br />
amount of virus and extend the duration of viral infectivity.<br />
Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-<br />
SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A<br />
DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-<br />
SIGN2) was identified recently (4-8). DC-SIGN forms a novel<br />
gene family with DC-SIGNR and many alternatively spliced<br />
isoforms of DC-SIGN and DC-SIGNR (8). The expression of<br />
DC-SIGN was found in mucosal tissues including placenta,<br />
small intestine, and rectum.<br />
SOURCE:<br />
Rabbit anti-DC-SIGN polyclonal antibody was raised against<br />
a synthetic peptide (CYFMSNSQRN WHDSITA)<br />
corresponding to amino acids 277 to 293 of human DC-<br />
DIGN (1).<br />
APPLICATION:<br />
This antibody can be used for detection of DC-SIGN by<br />
Western blot at 1 to 2 µg/ml. Human placenta lysate can be<br />
used as a positive control. A band at approximately 44 kDa<br />
can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2349P).<br />
Human placenta lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1309).<br />
Western blot analysis of DC-<br />
SIGN expression in human<br />
placenta tissue lysate in the<br />
absence (lane 1) and presence<br />
(lane 2) of blocking peptide with<br />
anti-DC-SIGN at 2 µg /ml.<br />
REFERENCES:<br />
1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a<br />
dendritic cell-specific HIV -1-binding protein that enhances transinfection<br />
of T cells. Cell. 2000;100:587-97.<br />
2. Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN<br />
interactions with human immunodeficiency virus type 1 and 2 and<br />
simian immunodeficiency virus. J Virol. 2001;75(10):4664-72.<br />
3. Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification<br />
of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that<br />
supports primary immune responses. Cell. 2000;100(5):575-85.<br />
4. Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene,<br />
DC-SIGNR; and CD23 form a cluster on 19p13. J Immunol.<br />
2000;165(6):2937-42.<br />
5. Pohlmann S, Soilleux EJ, Baribaud F, et al DC-SIGNR, a DC-<br />
SIGN homologue expressed in endothelial cells, binds to human<br />
and simian immunodeficiency viruses and activates infection in<br />
trans. Proc Natl Acad Sci USA. 2001;98(5):2670-2675.<br />
6. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, at el. A<br />
dendritic cell-specific intercellular adhesion molecule 3-grabbing<br />
nonintegrin (DC-SIGN)-related protein is highly expressed on<br />
human liver sinusoidal endothelial cells and promotes HIV -1<br />
infection. J Exp Med. 2001;193(6):671-8.<br />
7. Mitchell DA, Fadden AJ, Drickamer K. A novel mechanism of<br />
carbohydrate recognition by the C-type lectins DC-SIGN and DC-<br />
SIGNR: Subunit organisation and binding to multivalent ligands. J<br />
Biol Chem. 2001 in press<br />
8. Mummidi S, Catano G, Lam L, et al. Extensive repertoire of<br />
membrane-bound and soluble DC-SIGN1 and DC-SIGN2 isoforms:<br />
Inter-individual variation in expression of DC-SIGN transcripts. J<br />
Biol Chem. 2001 in press<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-NAK / TBK1 (CT)<br />
CATALOG No.: 2351<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli. NF-κB is associated with<br />
IκB proteins in the cell cytoplasm, which inhibit NF-κB<br />
activity. Phosphorylation of I-κB by IκB kinase (IKK) complex<br />
leads to degradation of I-κB and activation of NF-κB. The<br />
IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK<br />
related kinase was recently identified and designated TBK1<br />
(TANK-binding kinase 1), NAK (NF-κB-activating kinase),<br />
and T2K (1-3). NAK/TBK1 activates IKKβ through direct<br />
phosphorylation. NAK/TBK1 is activated by growth factors<br />
and PMA and mediates IKK and NF-kB activation in<br />
response to growth factors (2). NAK/TBK1 functions<br />
upstream of NIK and the IKK complex (1,2). NAK/TBK1 is<br />
also critical in protecting embryonic liver from apoptosis (3).<br />
SOURCE:<br />
Rabbit anti-NAK/TBK1 polyclonal antibody was raised<br />
against a synthetic peptide (ERFGSLTMDGGLRNVD)<br />
corresponding to amino acids 712 to 727 of human<br />
NAK/TBK1 (1,2), which are identical to those of mouse<br />
homologue (1).<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified<br />
IgG, 100 µg in 200 µl of PBS containing 0.02% sodium<br />
azide. Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2351P).<br />
REFERENCES:<br />
1. Pomerantz JL, Baltimore D. NF-kappaB activation by a<br />
signaling complex containing TRAF2, TANK and TBK1, a novel<br />
IKK-related kinase. EMBO J 1999;18(23):6694-704<br />
2. Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S,<br />
Nakayama K, Kaneko Y, Nimura Y, Motoyama N, Ikeda K, Karin<br />
M, Nakanishi M. NAK is an IkappaB kinase-activating kinase.<br />
Nature 2000;404(6779):778-82<br />
3. Bonnard M, Mirtsos C, Suzuki S, Graham K, Huang J, Ng M, Itie<br />
A, Wakeham A, Shahinian A, Henzel WJ, Elia AJ, Shillinglaw W,<br />
Mak TW, Cao Z, Yeh WC. Deficiency of T2K leads to apoptotic<br />
liver degeneration and impaired NF-kappaB-dependent gene<br />
transcription. EMBO J. 2000;19(18):4976-85.<br />
(WD0101)<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
NAK/TBK1 by Western blot at 0.5 to 1 µg/ml. MOLT4 whole<br />
cell lysate can be used as positive control and an 84 kDa<br />
band should be detected. It is human, mouse and rat<br />
reactive and has no cross response to IKKα, IKKβ, IKKγ, or<br />
IKKε. For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IRAK-M<br />
CATALOG No.: 2355<br />
BACKGROUND:<br />
Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces<br />
cellular response through IL-1 receptor (IL-1R) and Toll like<br />
receptors (TLR). IL-1 receptor-associated kinase (IRAK and<br />
IRAK2) mediates the activation of NF-κB by IL-1/Toll<br />
receptors (1,2), which is a pivotal transcription factor<br />
mediating inflammatory and immune response. A novel<br />
member in the IRAK/Pelle family was recently identified and<br />
designated IRAK-M (3). IRAKs associate with IL-1/Toll<br />
receptors after IL-1 or LPS stimulation and the dominant<br />
negative mutants of IRAKs inhibit IL-1 or LPS induced NFκB<br />
activation. Members in IRAK/Pelle family play a central<br />
role in IL-1R/TLR mediated inflammatory responses to<br />
cytokine IL-1 and LPS.<br />
STORAGE:<br />
It is supplied as chromatography purified IgG, 100 µg in<br />
200 µl of PBS containing 0.02% sodium azide. Store at<br />
4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2355P).<br />
Murine spleen tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1406).<br />
Western blot analysis of<br />
IRAK-M in mouse spleen (M)<br />
and rat liver (R) tissue lysates<br />
with anti-IRAK-M at 1 µg /ml.<br />
SOURCE:<br />
Rabbit anti-IRAK-M polyclonal antibody was raised against a<br />
peptide (CSSKFSWDEYEQYKKE) corresponding to amino<br />
acids 581 to 596 of human IRAK-M (3).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of IRAK-<br />
M by Western blot at 0.5 to 1 µg/ml. Mouse spleen or rat<br />
kidney tissue lysate can be used as positive control and a 68<br />
kDa major band can be detected. It is human, mouse, and<br />
rat reactive. Anti-IRAK-M has no cross response to IRAK or<br />
IRAK2. For research use only.<br />
REFERENCES:<br />
1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the<br />
interleukin-1 receptor. Science 1996;271:1128-31.<br />
2. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member<br />
IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.<br />
Science 1997;278:1612-5<br />
3. Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z. IRAK-<br />
M is a novel member of the Pelle/interleukin-1 receptor-associated<br />
kinase (IRAK) family. J Biol Chem. 1999;274(27):19403-10.<br />
(WD0101)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-p53R2 (NT)<br />
CATALOG No.: 2383<br />
BACKGROUND:<br />
The p53 tumor-suppressor gene integrates numerous<br />
signals that control cell life and death. Several novel<br />
molecules involved in p53 signaling, including p53R2 (1),<br />
Chk2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2<br />
(6), were recently discovered. p53R2 is a p53 inducible gene<br />
that contains a p53 binding sequence and encodes a subunit<br />
of the enzyme ribonucleotide reductase (1,7). p53R2 is<br />
induced by the reagents, ultraviolet and γ-irradiation that<br />
cause DNA damages. The product of p53R2 gene is directly<br />
involved in the p53 checkpoint for repair of damaged DNA.<br />
The isoform of the p53 family member p73 also induces<br />
p53R2 expression (8). p53R2 is an important target of p53<br />
for tumour suppression.<br />
SOURCE:<br />
Rabbit anti-p53R2 polyclonal antibody was raised against a<br />
synthetic peptide (GDPERPEAAGLDQDER) corresponding<br />
to amino acids 2 to 17 of human p53R2 (1).<br />
APPLICATION:<br />
This antibody can be used for detection of p53R2 by<br />
Western blot at 0.5 to 1 µg/ml. A431 or 3T3 cell lysate can<br />
be used as positive control and a 39 kDa band can be<br />
detected. It is human, mouse, and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2383P).<br />
A431 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1202).<br />
Western blot analysis of p53R2<br />
expression in A431 (A) and 3T3<br />
cell lysates in the absence (B) or<br />
presence (C) of blocking peptide<br />
with anti-p53R2 at 1 µg /ml.<br />
REFERENCES:<br />
1. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K,<br />
Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-<br />
dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.<br />
2. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle<br />
regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.<br />
3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T,<br />
Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and<br />
candidate mediator of p53-induced apoptosis. Science.<br />
2000;288(5468):1053-8.<br />
4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori<br />
H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator<br />
of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated<br />
p53. Cell. 2000 Sep 15;102(6):8 49-62.<br />
5. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53 modulates its<br />
effect on cell growth and apoptosis.<br />
Nature. 2000;408:377-81.<br />
6. Lin Y, Ma W, Benchimol S. Pidd, a new death-domain-containing protein,<br />
is induced by p53 and promotes apoptos is. Nat Genet. 2000;26:122-7.<br />
7. Lozano G, Elledge SJ. p53 sends nucleotides to repair DNA. Nature.<br />
2000;404:24-5.<br />
8. Nakano K, Balint E, Ashcroft M, Vousden KH. A ribonucleotide reductase<br />
gene is a transcriptional target of p53 and p73. Oncogene.<br />
2000;19(37):4283-9. (WD0105)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Chk2 (NT)<br />
CATALOG No.: 2391<br />
BACKGROUND:<br />
The p53 tumor-suppressor gene integrates numerous<br />
signals that control cell life and death. Several novel<br />
molecules involved in p53 signaling, including Chk2 (1),<br />
p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2<br />
(6), were recently discovered. The checkpoint kinase Chk2 is<br />
the mammalian homologue of yeast Cds1/Rad53. In<br />
response to DNA damage, the checkpoint kinase ATM<br />
phosphorylates and activates Chk2, which in turn directly<br />
phosphorylates and activates p53 (7,8). Chk2 serves as<br />
ATM downstream effector to mediate activation of p53. Chk2<br />
also phosphorylates and activates BRCA1, the product of a<br />
tumor suppressor gene that is mutated in breast and ovarian<br />
cancer (9).<br />
SOURCE:<br />
Rabbit anti-Chk2 polyclonal antibody was raised against a<br />
synthetic peptide (SRESDVEAQQSHGSSAC) corresponding<br />
to amino acids 2 to 18 of human Chk2 (1).<br />
APPLICATION:<br />
This antibody can be used for detection of Chk2 by Western<br />
blot at 0.5 to 1 µg/ml. Jurkat or K562 cell lysate can be used<br />
as positive control and a 60 kDa band can be detected. It is<br />
human, mouse, and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2391P).<br />
Jurkat cell lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1205).<br />
Western blot analysis of<br />
Chk2 expression in K562<br />
(A), Jurkat (B), and HL-60<br />
(C) whole cell lysates with<br />
anti-Chk2 at 1 µg /ml.<br />
REFERENCES:<br />
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell<br />
cycle regulation by the Chk2 protein kinase. Science.<br />
1998;282:1893-7.<br />
2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S,<br />
Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene<br />
involved in a p53-dependent cell-cycle checkpoint for DNA<br />
damage. Nature. 2000;404:42-9.<br />
3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita<br />
T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of<br />
the Bcl-2 family and candidate mediator of p53-induced<br />
apoptosis. Science. 2000;288(5468):1053-8.<br />
4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T,<br />
Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a<br />
potential mediator of p53-dependent apoptosis, and its regulation<br />
by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.<br />
5. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53<br />
modulates its effect on cell growth and apoptosis.<br />
Nature. 2000;408:377-81.<br />
6. Lin Y, Ma W, Benchimol S. Pidd, a new death-domaincontaining<br />
protein, is induced by p53 and promotes apoptosis.<br />
Nat Genet. 2000;26:122-7.<br />
7. Matsuoka S, Rotman G, Ogawa A, Shiloh Y, Tamai K, Elledge<br />
SJ. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo<br />
and in vitro.Proc Natl Acad Sci USA. 2000 ;97:10389-94.<br />
8. Hirao A, Kong YY, Matsuoka S, Wakeham A, Ruland J,<br />
Yoshida H, Liu D, Elledge SJ, Mak TW. DNA damage-induced<br />
activation of p53 by the checkpoint kinase Chk2. Science.<br />
2000;287:1824-7<br />
9. Lee JS, Collins KM, Brown AL, Lee CH, Chung JH. hCds1-<br />
mediated phosphorylation of BRCA1 regulates the DNA damage<br />
response. Nature. 2000;404:201-4.<br />
(WD0105)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Smac / DIABLO (CT)<br />
CATALOG No.: 2409<br />
BACKGROUND:<br />
The inhibitor of apoptosis proteins (IAPs) regulate<br />
programmed cell death by inhibiting members of the caspase<br />
family of enzymes. A novel mammalian protein that binds to<br />
IAPs and neutralizes the inhibitory effect of IAPs on<br />
caspases was recently identified and designated<br />
Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial<br />
protein that is released along with cytochrome c during<br />
apoptosis and activates cytochrome c/Apaf-1/capase-9<br />
pathway. Analysis of the structural basis of Smac/DIABLO<br />
reveals that the N-terminal amino acids are required for<br />
binding of Smac/DIABLO to IAPs and activation of caspases<br />
(3-6). Smac/DIABLO is expressed in a variety of human and<br />
mouse tissues (1,2).<br />
SOURCE:<br />
Rabbit anti-Smac/DIABLO (CT) polyclonal antibody was<br />
raised against a peptide (EERAESEQEAYLRED)<br />
corresponding to amino acids 225 to 239 of human<br />
Smac/DIABLO (1).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
Smac/DIABLO by Western blot at 0.5 to 1 µg/ml. Human<br />
heart tissue lysate can be used as positive control and a 25<br />
kDa band can be detected. It is human, mouse, and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2409P). Human heart tissue<br />
lysate, 200 µg at 2 mg/ml, is available for positive control<br />
(<strong>Catalog</strong> No. 1301).<br />
Western blot analysis of Smac<br />
in human heart tissue lysate in<br />
the absence (A) or presence (B)<br />
of blocking peptide (2409P) with<br />
anti-Smac at 1 µg /ml.<br />
REFERENCES:<br />
1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein<br />
that promotes cytochrome c-dependent caspase activation by<br />
eliminating IAP inhibition. Cell. 2000;102(1):33-42.<br />
2. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid<br />
GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a<br />
mammalian protein that promotes apoptosis by binding to and<br />
antagonizing IAP proteins. Cell. 2000;102(1):43-53.<br />
3. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z,<br />
Alnemri ES. Molecular Determinants of the Caspase-promoting<br />
Activity of Smac/DIABLO and Its Role in the Death Receptor<br />
Pathway. J Biol Chem. 2000;275(46):36152-36157.<br />
4. Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and<br />
biochemical basis of apoptotic activation by Smac/DIABLO. Nature.<br />
2000;406(6798):855-62.<br />
5. Liu Z, Sun C, Olejniczak ET, Meadows RP, Betz SF, Oost T,<br />
Herrmann J, Wu JC, Fesik SW. Structural basis for binding of<br />
Smac/DIABLO to the XIAP BIR3 domain. Nature.<br />
2000;408(6815):1004-8.<br />
6. Wu G, Chai J, Suber TL, Wu JW, Du C, Wang X, Shi Y. Structural<br />
basis of IAP recognition by Smac/DIABLO. Nature.<br />
2000;408(6815):1008-12.<br />
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Anti-mSmac / DIABLO (CT)<br />
CATALOG No.: 2411<br />
BACKGROUND:<br />
The inhibitor of apoptosis proteins (IAPs) regulate<br />
programmed cell death by inhibiting members of the caspase<br />
family of enzymes. A novel mammalian protein that binds to<br />
IAPs and neutralizes the inhibitory effect of IAPs on<br />
caspases was recently identified and designated<br />
Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial<br />
protein that is released along with cytochrome c during<br />
apoptosis and activates cytochrome c/Apaf-1/capase-9<br />
pathway. Analysis of the structural basis of Smac/DIABLO<br />
reveals that the N-terminal amino acids are required for<br />
binding of Smac/DIABLO to IAPs and activation of caspases<br />
(3-6). Smac/DIABLO is expressed in a variety of human and<br />
mouse tissues (1,2).<br />
SOURCE:<br />
Rabbit anti-mSmac/DIABLO (CT) polyclonal antibody was<br />
raised against a peptide (SDEGADQEEEAYLRED)<br />
corresponding to amino acids 222 to 237 of murine<br />
Smac/DIABLO (2).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of<br />
Smac/DIABLO by Western blot at 0.5 to 1 µg/ml. mouse<br />
heart tissue lysate can be used as positive control and a 25<br />
kDa band can be detected. It is human, mouse, and rat<br />
reactive. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2411P). Mouse heart<br />
tissue lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1401).<br />
Western blot analysis of Smac in<br />
mouse heart tissue lysate in the<br />
absence (A) or presence (B) of<br />
blocking peptide (2411P) and in<br />
rat heart tissue lysate with antimSmac<br />
at 1 µg /ml.<br />
REFERENCES:<br />
1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein<br />
that promotes cytochrome c-dependent caspase activation by<br />
eliminating IAP inhibition. Cell. 2000;102(1):33-42.<br />
2. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid<br />
GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a<br />
mammalian protein that promotes apoptosis by binding to and<br />
antagonizing IAP proteins. Cell. 2000;102(1):43-53.<br />
3. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang<br />
Z, Alnemri ES. Molecular Determinants of the Caspase-promoting<br />
Activity of Smac/DIABLO and Its Role in the Death Receptor<br />
Pathway. J Biol Chem. 2000;275(46):36152-36157.<br />
4. Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and<br />
biochemical basis of apoptotic activation by Smac/DIABLO.<br />
Nature. 2000;406(6798):855-62.<br />
5. Liu Z, Sun C, Olejniczak ET, Meadows RP, Betz SF, Oost T,<br />
Herrmann J, Wu JC, Fesik SW. Structural basis for binding of<br />
Smac/DIABLO to the XIAP BIR3 domain. Nature.<br />
2000;408(6815):1004-8.<br />
6. Wu G, Chai J, Suber TL, Wu JW, Du C, Wang X, Shi Y.<br />
Structural basis of IAP recognition by Smac/DIABLO. Nature.<br />
2000;408(6815):1008-12.<br />
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Anti-APRIL (ED2) TALL-2, TRDL-1<br />
CATALOG No.: 2415<br />
BACKGROUND:<br />
Members in the TNF superfamily regulate immune<br />
responses and induce apoptosis. A novel member in the<br />
TNF family was recently identified by several groups and<br />
designated APRIL (for a proliferation-inducing ligand), TALL-<br />
2 (for TNF- and ApoL-related Leukocyte-expressed Ligand<br />
2), and TRDL-1α (for TNF related death ligand 1α) in human<br />
and mouse (1-4). Two receptors for APRIL were recently<br />
identified and designated TACI and BCMA (5-7). APRIL<br />
stimulates B and T cell proliferation, triggers humoral<br />
immune responses, activates NF-kB, and induces cell death<br />
(1-7). APRIL and its close relative of BlyS and their<br />
receptors BCMA and TACI are involved in diseases of<br />
autoimmunity and cancer (8,9).<br />
SOURCE:<br />
Rabbit anti-APRIL (ED2) polyclonal antibody was raised<br />
against a synthetic peptide (CIRSMPSHPDRAYNS)<br />
corresponding to amino acids 196 to 210 of human APRIL<br />
(1-3).<br />
APPLICATION:<br />
This antibody can be used for detection of APRIL by<br />
Western blot at 2-4 µg/ml. HL60 or Jurkat cell lysate can be<br />
used as positive control and an approximately 42 kDa band<br />
can be detected. For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 200 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCT:<br />
Blocking peptide, 50 µg/250 µl, is available for competition<br />
studies (<strong>Catalog</strong> No. 2415P).<br />
HL60 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1209).<br />
Western blot analysis of APRIL<br />
expression in Jurkat cell lysates<br />
in the absence (A) or presence<br />
(B) of blocking peptide with anti-<br />
APRIL at 2 µg /ml.<br />
REFERENCES:<br />
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor<br />
necrosis factor family, stimulates tumor cell growth. J Exp Med.<br />
1998;188(6):1185-90.<br />
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF<br />
family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3.<br />
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis<br />
factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7.<br />
4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA<br />
and TACI: system for regulating humoral immunity. Nat Immunol.<br />
2000;1(3):252-6.<br />
5. Wu Y, Bressette D, Carrell JA, et a. Tumor necrosis factor (TNF) receptor<br />
superfamily member TACI is a high affinity receptor for TNF family members<br />
APRIL and BLyS. J Biol Chem. 2000;275(45):35478-85.<br />
6. Marsters SA, Yan M, Pitti RM, et al. Interaction of the TNF homologues<br />
BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr<br />
Biol. 2000;10(13):785-8.<br />
7. Rennert P, Schneider P, Cachero TG, at al. A soluble form of B cell<br />
maturation antigen, a receptor for the tumor necrosis factor family member<br />
APRIL, inhibits tumor cell growth. J Exp Med. 2000;192(11):1677-84.<br />
8. Gross JA, Johnston J, Mudri S, et al. TACI and BCMA are receptors for a<br />
TNF homologue implicated in B-cell autoimmune disease. Nature<br />
2000;404:995-9<br />
9. Ware CF. APRIL and BAFF connect autoimmunity and cancer. J Exp<br />
Med. 2000;192(11):F35-8.<br />
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Anti-PID / MTA2<br />
CATALOG No.: 2443<br />
BACKGROUND:<br />
The p53 tumor-suppressor gene integrates numerous<br />
signals that control cell life and death. Several novel<br />
molecules involved in p53 pathway, including Chk2 (1),<br />
p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2<br />
(6), were recently discovered. The transcriptional activity of<br />
p53 is modulated by protein stability and acetylation.<br />
PID/MTA2, also termed MTA1-L1, was found to be a subunit<br />
of nucleosome remodeling and deacetylating (NRD/NuRD)<br />
complex (6-8). PID/MTA2 modulates the enzymatic activity<br />
of the histone deacetylase complex and its expression<br />
reduces the levels of acetylated p53. Deacetylation of p53 by<br />
PID/MTA2 represses p53-dependent transcriptional<br />
activation and modulates p53-mediated cell growth arrest<br />
and apoptosis (6). PID/MTA2 is ubiquitously expressed in<br />
human tissues (8).<br />
SOURCE:<br />
Rabbit anti-PID/MTA2 polyclonal antibody was raised<br />
against a synthetic peptide (PAPSHPASTNEPIVLED)<br />
corresponding to amino acids 652 to 668 of human<br />
PID/MTA2 (6), which differ from the mouse sequence by one<br />
amino acid (7).<br />
APPLICATION:<br />
This antibody can be used for detection of PID/MTA2 by<br />
Western blot at 0.5 to 1 µg/ml. HeLa cell lysate can be used<br />
as positive control and a 75 kDa band can be detected. It is<br />
human, mouse, and rat reactive and has no cross response<br />
to MTA1. For research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2443P). HeLa cell lysate,<br />
200 µg at 2 mg/ml, is available for positive control (<strong>Catalog</strong><br />
No. 1201).<br />
Western blot analysis of<br />
PID/MTA2 expression in HeLa<br />
whole cell lysates in the absence<br />
(A) or presence (B) of blocking<br />
peptide with anti-PID at 1 µg /ml.<br />
REFERENCES:<br />
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle<br />
regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.<br />
2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S,<br />
Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene<br />
involved in a p53-dependent cell-cycle checkpoint for DNA<br />
damage. Nature. 2000;404:42-9.<br />
3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T,<br />
Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the<br />
Bcl-2 family and candidate mediator of p53-induced apoptosis.<br />
Science. 2000;288(5468):1053-8.<br />
4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T,<br />
Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a<br />
potential mediator of p53-dependent apoptosis, and its regulation<br />
by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.<br />
5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domaincontaining<br />
protein, is induced by p53 and promotes apoptosis. Nat<br />
Genet. 2000;26:122-7.<br />
6. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53<br />
modulates its effect on cell growth and apoptosis. Nature.<br />
2000;408:377-81.<br />
7. Zhang Y, Ng HH, Erdjument-Bromage H, Tempst P, Bird A,<br />
Reinberg D. Analysis of the NuRD subunits reveals a histone<br />
deacetylase core complex and a connection with DNA methylation.<br />
Genes Dev. 1999;13(15):1924-35.<br />
8. Futamura M, Nishimori H, Shiratsuchi T, Saji S, Nakamura Y,<br />
Tokino T.Molecular cloning, mapping, and characterization of a<br />
novel human gene, MTA1-L1, showing homology to a metastasisassociated<br />
gene, MTA1. J Hum Genet. 1999;44(1):52-6.<br />
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Anti-MTBP (IN2)<br />
CATALOG No.: 2447<br />
BACKGROUND:<br />
The p53 tumor-suppressor gene integrates numerous<br />
signals that control cell life and death. Several novel<br />
molecules involved in p53 network, including Chk2 (1),<br />
p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6)<br />
and MTBP (7), were recently discovered. The transcriptional<br />
activity of p53 is modulated by posttranslational regulations<br />
of the p53 protein including stabilization and acetylation. P53<br />
transcriptionally activates MDM2 gene then the translated<br />
MDM2 protein binds to p53 and promotes the degradation of<br />
p53 leading to lowering the concentration of p53 protein.<br />
MDM2 inhibits both p53 mediated G 1 arrest and apoptosis. A<br />
recently discovered protein termed MTBP was found to bind<br />
to MDM2 and to inhibit the modulation effect of MDM2 on<br />
p53 (7). MTBP is expressed in a variety of normal tissues<br />
(7).<br />
SOURCE:<br />
Rabbit anti-MTBP polyclonal antibody was raised against a<br />
synthetic peptide (GAVECFEEEDSNSRESLS)<br />
corresponding to amino acids 122 to 139 of human MTBP,<br />
which differ from the mouse sequence by three amino acids<br />
(7).<br />
APPLICATION:<br />
This antibody can be used for detection of MTBP by Western<br />
blot at 0.5 to 1 µg/ml. K562 cell lysate can be used as<br />
positive control and a 104 kDa band can be detected. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2447P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1204).<br />
Western blot analysis of<br />
MTBP expression in K562<br />
whole cell lysates with anti-<br />
MTBP at 1 µg /ml.<br />
REFERENCES:<br />
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle<br />
regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.<br />
2. Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S,<br />
Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene<br />
involved in a p53-dependent cell-cycle checkpoint for DNA<br />
damage. Nature. 2000;404:42-9.<br />
3. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T,<br />
Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the<br />
Bcl-2 family and candidate mediator of p53-induced apoptosis.<br />
Science. 2000;288(5468):1053-8.<br />
4. Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T,<br />
Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a<br />
potential mediator of p53-dependent apoptosis, and its regulation<br />
by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.<br />
5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domaincontaining<br />
protein, is induced by p53 and promotes apoptosis. Nat<br />
Genet. 2000;26:122-7.<br />
6. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53<br />
modulates its effect on cell growth and apoptosis. Nature.<br />
2000;408:377-81.<br />
7 Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein<br />
(MTBP) binds to MDM2 and induces a G1 arrest that is<br />
suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.<br />
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Anti-PERP<br />
CATALOG No.: 2451<br />
BACKGROUND:<br />
The p53 tumor-suppressor gene integrates numerous<br />
signals that control cell life and death. Several novel<br />
molecules involved in p53 network, including Chk2 (1),<br />
p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6),<br />
MTBP (7) and PERP (8), were identified and their genes<br />
were cloned recently. PERP, also termed PIGPC1 and THW,<br />
is a plasma membrane protein (8-10). p53 binds to the<br />
promoter of PERP and transcriptionally activates PERP gene<br />
then the translated PERP protein mediates the p53 induced<br />
apoptosis (8). The expression of PERP causes cell death.<br />
PERP is a mediator of p53 induced apoptosis. PERP has<br />
sequence similarity to PMP-22/gas3 and is a new member of<br />
the PMP-22/gas3 family (8).<br />
SOURCE:<br />
Rabbit anti-PERP polyclonal antibody was raised against a<br />
synthetic peptide (EDDLLGNAKPRYFYTSA) corresponding<br />
to amino acids 175 to 193 of human PERP, which differ from<br />
the mouse sequence by three amino acids (7-9).<br />
APPLICATION:<br />
This antibody can be used for detection of PERP by Western<br />
blot at 0.5 to 1 µg/ml. A431 cell lysate can be used as<br />
positive control and a 21 kDa band can be detected. For<br />
research use only.<br />
STORAGE:<br />
It is supplied as purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for one<br />
year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2451P).<br />
A431 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1202).<br />
Western blot analysis of<br />
PERP expression in A431<br />
whole cell lysates in the<br />
absence (A) and presence (B)<br />
of blocking peptide with anti-<br />
PERP at 1 µg /ml.<br />
REFERENCES:<br />
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle<br />
regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.<br />
2. Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide<br />
reductase gene involved in a p53-dependent cell-cycle checkpoint<br />
for DNA damage. Nature. 2000;404:42-9.<br />
3. Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of<br />
the Bcl-2 family and candidate mediator of p53-induced apoptosis.<br />
Science. 2000;288(5468):1053-8.<br />
4. Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential<br />
mediator of p53-dependent apoptosis, and its regulation by Ser-46-<br />
phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.<br />
5. Lin Y, Ma W, Benchimol S. Pidd, a new death-domaincontaining<br />
protein, is induced by p53 and promotes apoptosis. Nat<br />
Genet. 2000;26:122-7.<br />
6. Luo J, Su F, Chen D, et al. Deacetylation of p53 modulates its<br />
effect on cell growth and apoptosis. Nature. 2000;408:377-81.<br />
7 Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein<br />
(MTBP) binds to MDM2 and induces a G1 arrest that is<br />
suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.<br />
8. Attardi,L.D., Reczek,E.E., Cosmas,C., et al. PERP, an<br />
apoptosis-associated target of p53, is a novel member of the PMP-<br />
22/gas3 family. Genes Dev. 2000;14 (6):704-718<br />
9. Goltsov,A.A., Ren,C., Wang,J., et al. p53 regulates the<br />
expression of a novel four transmembrane protein-PERP/PIGPC1<br />
in mouse and human prostate cancer. GeneBank database<br />
10. Hildebrandt,T., Weidle,U., Preiherr,J. et al. Identification of<br />
THW, a putative new tumor suppressor gene. GeneBank database<br />
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Anti-IL-21 (IN)<br />
CATALOG No.: 2465<br />
BACKGROUND:<br />
A novel cytokine was recently identified in human and mouse<br />
and designated IL-21 (1), which has significant homology to<br />
IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also<br />
termed NILR for novel Interleukin receptor) is a new member<br />
of the class I cytokine receptor family (1,2). IL-21R forms a<br />
complex with the common cytokine receptor γ chain, γ c , and<br />
mediates IL-21 signaling (3,4). IL-21 and its receptor activate<br />
JAK-STAT signaling pathway. IL-21 is expressed in activated<br />
T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in<br />
the proliferation and maturation of NK, B and T cell<br />
populations.<br />
SOURCE:<br />
Rabbit anti-IL-21 polyclonal antibody was raised against a<br />
synthetic peptide (TCPSCDSYEKKPPKE) corresponding to<br />
amino acids 121 to 135 of human IL-21 precursor (1).<br />
APPLICATION:<br />
This antibody can be used for detection of IL-21 by Western<br />
blot at 0.5 to 1 µg/ml. Human HL-60 and THP-1 cell lysates<br />
can be used as positive control and an approximately 18 kDa<br />
band can be detected.<br />
For research use only.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2465P).<br />
Human HL-60 cell lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1209).<br />
Western blot analysis of IL-21<br />
expression in HL-60 cell lysate in<br />
the absence (lane A) or presence<br />
of blocking peptide (lane B) with<br />
anti-IL-21 (IN) at 1 µg /ml.<br />
REFERENCES:<br />
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and<br />
its receptor are involved in NK cell expansion and regulation of<br />
lymphocyte function. Nature. 2000;408(6808):57-63.<br />
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I<br />
cytokine receptor most related to the IL-2 receptor beta chain. Proc<br />
Natl Acad Sci USA. 2000;97(21):11439-44.<br />
3. Asao H, Okuyama C, Kumaki S, et al. the common gammachain<br />
is an indispensable subunit of the IL-21 receptor complex. J<br />
Immunol. 2001;167(1):1-5.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
4. Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the<br />
gamma(c)-dependent network? Curr Biol. 2001;11(5):R175-7.<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IL-21R (ED)<br />
CATALOG No.: 2469<br />
BACKGROUND:<br />
A novel cytokine related to IL-2 and IL-15 was recently<br />
identified and designated IL-21 (1). The receptor for IL-21<br />
(IL-21R, also termed NILR for novel Interleukin receptor) is a<br />
new member of the class I cytokine receptor family (1,2). IL-<br />
21R forms a complex with the common cytokine receptor γ<br />
chain, γ c , and mediates IL-21 signaling (3,4). Both IL-21R<br />
and the γ c are necessary for the IL-21 function. IL-21 and its<br />
receptor activate JAK-STAT signaling pathway. IL-21R is<br />
expressed in spleen, thymus, natural killer (NK), T and B cell<br />
lines. IL-21 plays a role in the proliferation and maturation of<br />
NK, B and T cell populations.<br />
SOURCE:<br />
Rabbit anti-IL-21R polyclonal antibody was raised against a<br />
synthetic peptide (NITDQSGNYSQECGS) corresponding to<br />
amino acids 97 to 111 of human IL-21R precursor (1).<br />
APPLICATION:<br />
This antibody can be used for detection of IL-21R by<br />
Western blot at 0.5 to 1 µg/ml. Human Raji and A431 cell<br />
lysates can be used as positive control and an approximately<br />
60 kDa band can be detected.<br />
For research use only.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2469P).<br />
Human Raji cell lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1207).<br />
Western blot analysis of IL-21R<br />
expression in human Raji cell<br />
lysate with anti-IL-21R (ED) at 1<br />
µg /ml.<br />
REFERENCES:<br />
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and<br />
its receptor are involved in NK cell expansion and regulation of<br />
lymphocyte function. Nature. 2000;408(6808):57-63.<br />
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I<br />
cytokine receptor most related to the IL-2 receptor beta chain. Proc<br />
Natl Acad Sci USA. 2000;97(21):11439-44.<br />
3. Asao H, Okuyama C, Kumaki S, et al. the common gammachain<br />
is an indispensable subunit of the IL-21 receptor complex. J<br />
Immunol. 2001;167(1):1-5.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
4. Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the<br />
gamma(c)-dependent network? Curr Biol. 2001;11(5):R175-7.<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IL-21R (NT)<br />
CATALOG No.: 2471<br />
BACKGROUND:<br />
A novel cytokine related to IL2 and IL15 was recently<br />
identified and designated IL-21 (1). The receptor for IL-21<br />
(IL-21R, also termed NILR for novel Interleukin receptor) is a<br />
new member of the class I cytokine receptor family (1,2). IL-<br />
21R forms a complex with the common cytokine receptor γ<br />
chain, γ c , and mediates IL-21 signaling (3,4). Both IL-21R<br />
and the γ c are necessary for the IL-21 function. IL-21 and its<br />
receptor activate JAK-STAT signaling pathway. IL-21R is<br />
expressed in spleen, thymus, natural killer (NK), T and B cell<br />
lines. IL-21 plays a role in the proliferation and maturation of<br />
NK, B and T cell populations.<br />
SOURCE:<br />
Rabbit anti-IL-21R polyclonal antibody was raised against a<br />
synthetic peptide (CPDLVCYTDYLQT) corresponding to<br />
amino acids 20 to 32 of human IL-21R precursor (1).<br />
APPLICATION:<br />
This antibody can be used for detection of IL-21R by<br />
Western blot at 0.5 to 1 µg/ml. Human Raji and A431 cell<br />
lysates can be used as positive control and an approximately<br />
60 kDa band can be detected.<br />
For research use only.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2471P).<br />
Human Raji cell lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1207).<br />
Western blot analysis of IL-<br />
21R expression in human Raji<br />
cell lysate with anti-IL-21R<br />
(NT) at 1 µg /ml.<br />
REFERENCES:<br />
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and<br />
its receptor are involved in NK cell expansion and regulation of<br />
lymphocyte function. Nature. 2000;408(6808):57-63.<br />
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I<br />
cytokine receptor most related to the IL-2 receptor beta chain. Proc<br />
Natl Acad Sci USA. 2000;97(21):11439-44.<br />
3. Asao H, Okuyama C, Kumaki S, et al. the common gammachain<br />
is an indispensable subunit of the IL-21 receptor complex. J<br />
Immunol. 2001;167(1):1-5.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
4. Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the<br />
gamma(c)-dependent network? Curr Biol. 2001;11(5):R175-7.<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-TCCR (NT)<br />
CATALOG No.: 2481<br />
BACKGROUND:<br />
Upon antigen challenge, T-helper cells differentiate into two<br />
functional distinct subsets, Th1 and Th2. Th1 cells produce<br />
IL-2, IFN-γ and lymphotoxin-β that augment cell mediated<br />
immune response while Th2 cells secrete IL-4, IL-5, and IL-<br />
10 that enhance humoral immunity. The function of T-helper<br />
cells is regulated by cytokines. A novel cytokine receptor<br />
was recently identified and cloned (1,2). It is a new member<br />
in the type I cytokine receptor family and designated TCCR<br />
for T-cell cytokine receptor and WSX-1 (1,2). TCCR<br />
deficient mice had impaired Th1 responses to protein<br />
antigen challenge, including decreased levels of IFN-γ and<br />
Th1-dependent antibody IgG2a (1). TCCR is predominately<br />
expressed in thymus, spleen, lymph notes and peripheral<br />
blood leukocytes.<br />
SOURCE:<br />
Rabbit anti-TCCR polyclonal antibody was raised against a<br />
synthetic peptide (GSAGPLQCYGVGPLGD)<br />
corresponding to amino acids 44 to 59 of human TCCR<br />
precursor (1),<br />
APPLICATION:<br />
This antibody can be used for detection of TCCR by Western<br />
blot at 0.5 to 1 µg/ml. Human spleen tissue lysate can be<br />
used as positive control and an approximately 70 kDa band<br />
can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
Western blot analysis of<br />
TCCR expression in human<br />
spleen tissue lysates with<br />
anti-TCCR at 1 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2481P).<br />
K562 cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1306).<br />
REFERENCES:<br />
1. Chen Q, Ghilardi N, Wang H, Baker T, Xie MH, Gurney A,<br />
Grewal IS and de Sauvage FJ. Development of Th1-type immune<br />
responses requires the type I cytokine receptor TCCR Nature<br />
2000;407(6806):916-920<br />
2. Sprecher,C.A., Grant,F.J., Baumgartner,J.W., Presnell,S.R.,<br />
Schrader,S.K., Yamagiwa,T., Whitmore,T.E., O'Hara,P.J. and<br />
Foster,D.F. Cloning and characterization of a novel class I cytokine<br />
receptor Biochem. Biophys. Res. Commun. 1998;246(1):82-90<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-TCCR (CT)<br />
CATALOG No.: 2483<br />
BACKGROUND:<br />
Upon antigen challenge, T-helper cells differentiate into two<br />
functional distinct subsets, Th1 and Th2. Th1 cells produce<br />
IL-2, IFN-γ and lymphotoxin-β that augment cell mediated<br />
immune response while Th2 cells secrete IL-4, IL-5, and IL-<br />
10 that enhance humoral immunity. The function of T-helper<br />
cells is regulated by cytokines. A novel cytokine receptor<br />
was recently identified and cloned (1,2). It is a new member<br />
in the type I cytokine receptor family and designated TCCR<br />
for T-cell cytokine receptor and WSX-1 (1,2). TCCR<br />
deficient mice had impaired Th1 responses to protein<br />
antigen challenge, including decreased levels of IFN-γ and<br />
Th1-dependent antibody IgG2a (1). TCCR is predominately<br />
expressed in thymus, spleen, lymph notes and peripheral<br />
blood leukocytes.<br />
SOURCE:<br />
Rabbit anti-TCCR polyclonal antibody was raised against a<br />
synthetic peptide (SGYEKHFLPTPEELG) corresponding to<br />
amino acids 611 to 625 of human TCCR precursor (1). This<br />
sequence is identical to that of mouse TCCR (1,2)<br />
APPLICATION:<br />
This antibody can be used for detection of TCCR by Western<br />
blot at 0.5 to 1 µg/ml. Human spleen tissue lysate can be<br />
used as positive control and an approximately 70 kDa band<br />
can be detected. It is human, mouse, and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
Western blot analysis of<br />
TCCR expression in human<br />
spleen tissue lysate with anti-<br />
TCCR-CT at 1 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2483P).<br />
Human spleen tissue lysate, 200 µg at 2 mg/ml, is available<br />
for positive control (<strong>Catalog</strong> No. 1306).<br />
REFERENCES:<br />
1. Chen Q, Ghilardi N, Wang H, Baker T, Xie MH, Gurney A,<br />
Grewal IS and de Sauvage FJ. Development of Th1-type immune<br />
responses requires the type I cytokine receptor TCCR Nature<br />
2000;407(6806):916-920<br />
2. Sprecher,C.A., Grant,F.J., Baumgartner,J.W., Presnell,S.R.,<br />
Schrader,S.K., Yamagiwa,T., Whitmore,T.E., O'Hara,P.J. and<br />
Foster,D.F. Cloning and characterization of a novel class I cytokine<br />
receptor Biochem. Biophys. Res. Commun. 1998;246(1):82-90<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IL-22R (CT)<br />
CATALOG No.: 2489<br />
BACKGROUND:<br />
A novel cytokine, designated IL-TIF for IL-10 related T cellderived<br />
inducible factor and IL-22, was recently identified (1-<br />
3). The receptor for IL-22 (IL-22R, also termed CRF2-9 and<br />
IL-TIF-R1 chain) is a new member of the class II cytokine<br />
receptor family (3,4). IL-22R forms a complex with IL-10<br />
receptor β chain and mediates IL-22 signaling. IL-22 and its<br />
receptor activate JAK-STAT signaling pathway. IL22R is<br />
expressed in normal liver and kidney and their cell lines<br />
HepG2 and TK-10. A soluble form of IL-22 receptor, also<br />
termed IL-22 binding protein (IL-22BP) and IL-22 receptor-α<br />
2 (IL-22RA2), was identified very recently (5-7). IL-22BP<br />
prevents binding of IL-22 to the functional cell surface IL-22R<br />
complex and neutralizes IL-22 activity. LPS induces IL-22<br />
expression, which indicates the role of IL-22 in inflammatory<br />
response.<br />
SOURCE:<br />
Rabbit anti-IL-22R polyclonal antibody was raised against a<br />
synthetic peptide (DSLFRGLALTVQWES) corresponding to<br />
amino acids 560 to 574 of human IL-22R precursor (1).<br />
APPLICATION:<br />
This antibody can be used for detection of IL-22R by<br />
Western blot at 0.5 to 1 µg/ml. Human HepG2 cell lysate can<br />
be used as positive control and an approximately 62 kDa<br />
band can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2489P). Human HepG2<br />
cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1211).<br />
Western blot analysis of IL-<br />
22R expression in human<br />
HepG2 cell lysate with anti-<br />
IL-22R (CT) at 1 µg /ml.<br />
REFERENCES:<br />
1. Dumoutier L, Louahed J, Renauld JC. Cloning and<br />
characterization of IL-10-related T cell-derived inducible factor (IL-<br />
TIF), a novel cytokine structurally related to IL-10 and inducible by<br />
IL-9. J Immunol. 2000;164(4):1814-9.<br />
2. Dumoutier L, Van Roost E, Colau D, Renauld JC. Human<br />
interleukin-10-related T cell-derived inducible factor: molecular<br />
cloning and functional characterization as an hepatocytestimulating<br />
factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.<br />
3. Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel<br />
human cytokine that signals through the interferon receptor-related<br />
proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.<br />
4. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al.<br />
Identification of the functional interleukin-22 (IL-22) receptor<br />
complex: J Biol Chem. 2001;276(4):2725-32.<br />
5. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al.<br />
Identification, cloning, and characterization of a novel soluble<br />
receptor that binds IL-22 and neutralizes its activity. J Immunol.<br />
2001;166(12):7096-103.<br />
6. Dumoutier L, Lejeune D, Colau D, Renauld JC. Cloning and<br />
characterization of il-22 binding protein, a natural antagonist of il-<br />
10-related t cell-derived inducible factor/il-22. J Immunol.<br />
2001;166(12):7090-5.<br />
7. Xu W, Presnell SR, Parrish-Novak J, et al. A soluble class II<br />
cytokine receptor, IL-22RA2, is a naturally occurring IL-22<br />
antagonist. Proc Natl Acad Sci USA. 2001 in press (WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-IL-22R (NT)<br />
CATALOG No.: 2497<br />
BACKGROUND:<br />
A novel cytokine, designated IL-TIF for IL-10 related T cellderived<br />
inducible factor and IL-22, was recently identified (1-<br />
3). The receptor for IL-22 (IL-22R, also termed CRF2-9 and<br />
IL-TIF-R1 chain) is a new member of the class II cytokine<br />
receptor family (3,4). IL-22R forms a complex with IL-10<br />
receptor β chain and mediates IL-22 signaling. IL-22 and its<br />
receptor activate JAK-STAT signaling pathway. IL22R is<br />
expressed in normal liver and kidney and their cell lines<br />
HepG2 and TK-10. A soluble form of IL-22 receptor, also<br />
termed IL-22 binding protein (IL-22BP) and IL-22 receptor-α<br />
2 (IL-22RA2), was identified very recently (5-7). IL-22BP<br />
prevents binding of IL-22 to the functional cell surface IL-22R<br />
complex and neutralizes IL-22 activity. LPS induces IL-22<br />
expression, which indicates the role of IL-22 in inflammatory<br />
response.<br />
SOURCE:<br />
Rabbit anti-IL-22R polyclonal antibody was raised against a<br />
synthetic peptide (QSSNFENILTWDSGPE) corresponding to<br />
amino acids 31 to 46 of human IL-22R precursor (1).<br />
APPLICATION:<br />
This antibody can be used for detection of IL-22R by<br />
Western blot at 0.5 to 1 µg/ml. Human HepG2 cell lysate can<br />
be used as positive control and an approximately 62 kDa<br />
band can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2497P). Human HepG2<br />
cell lysate, 200 µg at 2 mg/ml, is available for positive<br />
control (<strong>Catalog</strong> No. 1211).<br />
Western blot analysis of IL-22R<br />
expression in human HepG2 cell<br />
lysate with anti-IL-22R (NT) at 1<br />
µg /ml.<br />
REFERENCES:<br />
1. Dumoutier L, Louahed J, Renauld JC. Cloning and<br />
characterization of IL-10-related T cell-derived inducible factor (IL-<br />
TIF), a novel cytokine structurally related to IL-10 and inducible by<br />
IL-9. J Immunol. 2000;164(4):1814-9.<br />
2. Dumoutier L, Van Roost E, Colau D, Renauld JC. Human<br />
interleukin-10-related T cell-derived inducible factor: molecular<br />
cloning and functional characterization as an hepatocytestimulating<br />
factor. Proc Natl Acad Sci USA. 2000 ;97(18):10144-9.<br />
3. Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel<br />
human cytokine that signals through the interferon receptor-related<br />
proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.<br />
4. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al.<br />
Identification of the functional interleukin-22 (IL-22) receptor<br />
complex: J Biol Chem. 2001;276(4):2725-32.<br />
5. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al.<br />
Identification, cloning, and characterization of a novel soluble<br />
receptor that binds IL-22 and neutralizes its activity. J Immunol.<br />
2001;166(12):7096-103.<br />
6. Dumoutier L, Lejeune D, Colau D, Renauld JC. Cloning and<br />
characterization of il-22 binding protein, a natural antagonist of il-<br />
10-related t cell-derived inducible factor/il-22. J Immunol.<br />
2001;166(12):7090-5.<br />
7. Xu W, Presnell SR, Parrish-Novak J, et al. A soluble class II<br />
cytokine receptor, IL-22RA2, is a naturally occurring IL-22<br />
antagonist. Proc Natl Acad Sci USA. 2001 in press (WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Act1 / CIKS (CT)<br />
CATALOG No.: 2499<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli. NF-κB associates with IκB<br />
proteins in the cell cytoplasm, which inhibit NF-κB activity.<br />
IκB is phosphorylated by IκB kinase (IKK) complex that<br />
contains IKKα, IKKβ, and IKKγ. A novel molecule that<br />
associates with and activates IKK was recently identified and<br />
designated CIKS (for connection to IKK and SAPK/JNK) and<br />
Act1 (for NF-κB activator 1) (1,2). CIKS directly interacts with<br />
IKKγ. CIKS/Act1 also activates activating transcription factor<br />
(ATF) and activator protein 1 (AP-1) through Jun kinase<br />
(JNK). These results indicate that CIKS/Act1 is involved in<br />
the inflammation and stress responses. CIKS/Act1 is<br />
ubiquitously expressed in human tissues.<br />
SOURCE:<br />
Rabbit anti-CIKS polyclonal antibody was raised against a<br />
synthetic peptide (REEEYVAPPRGPLPT) corresponding to<br />
amino acids 554 to 568 of human CIKS (1,2).<br />
APPLICATION:<br />
This antibody can be used for detection of CIKS by Western<br />
blot at 0.5 to 1 µg/ml. Human placenta tissue lysate can be<br />
used as a positive control. A band at approximately 63 kDa<br />
can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
Western blot analysis of CIKS<br />
expression in human placenta<br />
tissue lysate with anti-CIKS (CT)<br />
at 1 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2499P).<br />
Human placenta tissue lysate, 200 µg at 2 mg/ml, is<br />
available for positive control (<strong>Catalog</strong> No. 1309).<br />
REFERENCES:<br />
1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U.<br />
CIKS, a connection to Ikappa B kinase and stress-activated protein<br />
kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.<br />
2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D,<br />
Haag M, Stark GR. Act1, an NF-kappa B activating protein. Proc<br />
Natl Acad Sci USA. 2000;97(19):10489-93.<br />
(WD0108)
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Act1 / CIKS (NT)<br />
CATALOG No.: 2501<br />
BACKGROUND:<br />
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription<br />
factor and an essential mediator of gene expression during<br />
activation of immune and inflammatory responses. NF-κB<br />
mediates the expression of a great variety of genes in<br />
response to extracellular stimuli. NF-κB associates with IκB<br />
proteins in the cell cytoplasm, which inhibit NF-κB activity.<br />
IκB is phosphorylated by IκB kinase (IKK) complex that<br />
contains IKKα, IKKβ, and IKKγ. A novel molecule that<br />
associates with and activates IKK was recently identified and<br />
designated CIKS (for connection to IKK and SAPK/JNK) and<br />
Act1 (for NF-κB activator 1) (1,2). CIKS directly interacts with<br />
IKKγ. CIKS/Act1 also activates activating transcription factor<br />
(ATF) and activator protein 1 (AP-1) through Jun kinase<br />
(JNK). These results indicate that CIKS/Act1 is involved in<br />
the inflammation and stress responses. CIKS/Act1 is<br />
ubiquitously expressed in human tissues.<br />
SOURCE:<br />
Rabbit anti-CIKS polyclonal antibody was raised against a<br />
synthetic peptide (PPQLQETRMNRSIP) corresponding to<br />
amino acids 2 to 15 of human CIKS (1,2).<br />
APPLICATION:<br />
This antibody can be used for detection of CIKS by Western<br />
blot at 0.5 to 1 µg/ml. Human placenta tissue lysate can be<br />
used as a positive control. A band at approximately 63 kDa<br />
can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200<br />
µl of PBS containing 0.02% sodium azide. Store at 4°C,<br />
stable for one year.<br />
Western blot analysis of<br />
CIKS expression in human<br />
lung (lane A) and placenta<br />
(lane B) tissue lysates with<br />
anti-CIKS (NT) at 1 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2501P).<br />
Human placenta tissue lysate, 200 µg at 2 mg/ml, is<br />
available for positive control (<strong>Catalog</strong> No. 1309).<br />
REFERENCES:<br />
1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U.<br />
CIKS, a connection to Ikappa B kinase and stress-activated protein<br />
kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.<br />
2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D,<br />
Haag M, Stark GR. Act1, an NF-kappa B activating protein. Proc<br />
Natl Acad Sci USA. 2000;97(19):10489-93.<br />
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Anti-Livin, KIAP<br />
CATALOG No.: 2505<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, is related to many<br />
diseases, such as cancer. Apoptosis is triggered by a variety<br />
of stimuli including members in the TNF family and<br />
prevented by the inhibitor of apoptosis (IAP) proteins. IAP<br />
proteins form a conserved gene family that binds to and<br />
inhibits cell death proteases. A novel member in the IAP<br />
protein family was recently identified and designated Livin<br />
and KIAP for kidney IAP (1,2). Livin/XIAP contains a single<br />
baculoviral IAP repeat (BIR) domain and a RING finger<br />
domain and has two isoforms termed Livin-α and Livin-β<br />
(1,3). Transfection of Livin in cells resulted in protection from<br />
apoptosis induced by FADD, BAX, RIP, RIP3 and DR6 (1).<br />
Livin has direct interaction with several caspases including<br />
caspase-3, -7, and –9. Livin inhibits the activation of<br />
caspase-9 induced by Apaf-1, cytochrome c, and dATP. The<br />
two isoforms of Livin ???????to ?????different function<br />
and tissue distribution.<br />
SOURCE:<br />
Rabbit anti-Livin polyclonal antibody was raised with a<br />
synthetic peptide (CRAPVRSRVRTFLS) corresponding to<br />
amino acids 264 to 280 of the short form and 281 to 298 of<br />
the long form of human Livin (1,3). This sequence is identical<br />
between α and β ????s ?? the Livin ????????.<br />
APPLICATION:<br />
This antibody can be used for detection of Livin by Western<br />
blot at 2 to 4 µg/ml. Human Raji cell lysate can be used as a<br />
positive control and a band at 33 kDa can be detected. A<br />
lower but much weaker band at 30 kDa was detected in Raji<br />
cell lysate, which may represent the short<br />
??????????????<br />
For research use only.<br />
Western blot analysis of Livin<br />
expression in human Raji cell lysate with<br />
anti-Livin at 4 µg/ml.<br />
STORAGE: It is supplied as immunoaffinity<br />
chromatography purified IgG, 100 µg in 200 µl of PBS<br />
containing 0.02% sodium azide. Store at 4°C, stable for<br />
one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2505P).<br />
Human Raji cell lysate (<strong>Catalog</strong> No. 1207), 200 µg at 2<br />
mg/ml, is available for positive control.<br />
REFERENCES:<br />
1. Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis<br />
protein family member. J Biol Chem. 2001;276(5):3238-46.<br />
2. Lin JH, Deng G, Huang Q, Morser J. KIAP, a novel member of<br />
the inhibitor of apoptosis protein family. Biochem Biophys Res<br />
Commun. 2000;279(3):820-31.<br />
3. Ashhab Y, Alian A, Polliack A, Panet A, Yehuda DB.<br />
Two splicing variants of a new inhibitor of apoptosis gene with<br />
different biological properties and tissue distribution pattern.<br />
FEBS Lett. 2001;495(1-2):56-60.<br />
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Anti-CARD9<br />
CATALOG No.: 2515<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development.<br />
Cell death signals are transduced by death domain (DD),<br />
death effector domain (DED), and caspase recruitment<br />
domain (CARD) containing molecules. CARD containing<br />
proteins include some caspases, Apaf-1, CARD4, IAPs,<br />
RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARDcontaining<br />
protein was recently identified and designated<br />
CARD9, which interacts with the CARD activation domain of<br />
BCL-10 (1). CARD9 associates with BCL-10 and forms a<br />
complex within cells. CARD9 induces apoptosis and<br />
activates NF-κB. CARD9 is an upstream activator of BCL-10<br />
and NF-κB signaling.<br />
SOURCE:<br />
Rabbit anti-CARD9 polyclonal antibody was raised against a<br />
synthetic peptide (DRENTTGSDNTDTEGS) corresponding to<br />
amino acids 521 to 536 of human CARD9 (1). The<br />
sequence is different from that of rat origin by two amino<br />
acids (1).<br />
APPLICATION:<br />
This antibody can be used for detection of CARD9 by<br />
Western blot at 0.5 to 1 µg/ml. Human PC-3 and MDA-MB-<br />
361 cell lysates can be used as a positive control and a band<br />
at approximately 59 kDa can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity purified IgG, 100 µg in 200 µl<br />
of PBS containing 0.02% sodium azide. Store at 4°C, stable<br />
for one year.<br />
Western blot analysis of CARD9<br />
expression in human MDA-MB-361<br />
(A) and PC-3 (B) cell lysate with anti-<br />
CARD9 at 2.5 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 2515P).<br />
Human PC-3 lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1216).<br />
REFERENCES:<br />
1. Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL,<br />
Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES.<br />
CARD9 is a novel caspase recruitment domain-containing protein that<br />
interacts with BCL10/CLAP and activates NF-kappa B. J Biol Chem.<br />
2000;275(52):41082-6.<br />
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Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-ILP-2<br />
CATALOG No.: 3017<br />
BACKGROUND:<br />
Apoptosis, or programmed cell death, is related to many<br />
diseases, such as cancer. Apoptosis is triggered by a variety<br />
of stimuli including members in the TNF family and<br />
prevented by the inhibitor of apoptosis (IAP) proteins. IAP<br />
proteins form a conserved gene family including IAP,<br />
XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits<br />
specific proteases. A novel member in the IAP protein family<br />
was recently identified and designated ILP-2 for IAP-like<br />
protein-2 (1). ILP-2 has high homology to ILP-1, but is<br />
encoded by a distinct gene that is solely expressed in testis<br />
of tested normal human tissues (1). ILP-2, unlike ILP-1, has<br />
no inhibitory effect on Fas and TNF induced apoptosis, but<br />
potently inhibits apoptosis induced by overexpression of Bax<br />
or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts<br />
with the processed caspase-9. These results suggest that<br />
ILP-2 is a novel IAP family member with restricted specificity<br />
for caspase-9.<br />
SOURCE:<br />
Rabbit anti-ILP-2 polyclonal antibody was raised with a<br />
synthetic peptide (TGYEARLITFGT) corresponding to amino<br />
acids 2 to 13 of human ILP-2 (1).<br />
APPLICATION:<br />
This antibody can be used for detection of ILP-2 by Western<br />
blot at 1 to 2 µg/ml. Human HepG2 or MOLT4 cell lysate<br />
can be used as a positive control and a band at<br />
approximately 33 kDa can be detected. It is human, mouse,<br />
and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of ILP-2<br />
expression in human HepG2 (lane 1)<br />
and MOLT4 (lane 2) cell lysates and<br />
mouse spleen (lane 3) tissue lysates<br />
with anti-ILP-2 at 1 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3017P).<br />
Human HepG2 cell lysate (<strong>Catalog</strong> No. 1211), 200 µg at 2<br />
mg/ml, is available for positive control.<br />
REFERENCES:<br />
1. Richter BW, Mir SS, Eiben LJ, Lewis J, Reffey SB, Frattini A, Tian<br />
L, Frank S, Youle RJ, Nelson DL, Notarangelo LD, Vezzoni P,<br />
Fearnhead HO, Duckett CS. Molecular cloning of ILP-2, a novel<br />
member of the inhibitor of apoptosis protein family. Mol Cell Biol.<br />
2001;21(13):4292-301.<br />
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Anti-ARTS<br />
CATALOG No.: 3025<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development.<br />
Mitochondrial proteins, such as cytochrome c, Apaf-1, and<br />
AIF play important role in apoptosis. A novel mitochondrial<br />
septin-like protein was identified recently and designated<br />
ARTS for apoptosis related protein in TGF-β signaling<br />
pathway (1). ARTS that is encoded by the human septin<br />
H5/PNUTL2/CDCrel2b gene (1-4) is located to mitochondria<br />
and translocates to the nucleus when apoptosis occurs.<br />
ARTS is expressed in many tissues. It enhances cell death<br />
induced by TGF-β and, to a lesser extent, by other apoptotic<br />
agents, such as TNF-α and Fas ligand.<br />
SOURCE:<br />
Rabbit anti-ARTS polyclonal antibody was raised against a<br />
synthetic peptide (KRFLEDTTDDGE) corresponding to<br />
amino acids 3 to 14 of human ARTS (1).<br />
APPLICATION:<br />
This antibody can be used for detection of ARTS by Western<br />
blot at 0.5 to 2 µg/ml. Human lung, kidney and spleen tissue<br />
lysates can be used as a positive control and a band at 32<br />
kDa can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3025P).<br />
Human lung tissue lysate, 200 µg at 2 mg/ml, is available for<br />
positive control (<strong>Catalog</strong> No. 1302).<br />
Western blot analysis of ARTS<br />
expression in human lung (lane 1),<br />
spleen (lane 2), and kidney (lane 3)<br />
tissue lysates with anti-ARTS at 2 µg /ml.<br />
REFERENCES:<br />
1. Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial<br />
septin-like protein, ARTS, mediates apoptosis dependent on its P-<br />
loop motif. Nat Cell Biol 2000;2(12):915-21<br />
2. Depraetere V. The ARTS of apoptosis. Nat Cell Biol.<br />
2000;2(12):E219.<br />
3. Paavola,P., Horelli-Kuitunen,N., Palotie,A. and Peltonen,L.<br />
Characterization of a novel gene, PNUTL2, on human chromosome<br />
17q22-q23 and its exclusion as the Meckel syndrome gene.<br />
Genomics 1999;55 (1):122-125<br />
4. Zieger,B., Tran,H., Hainmann,I., Wunderle,D., Zgaga-Griesz,A.,<br />
Blaser,S. and Ware,J. Characterization and expression analysis of<br />
two human septin genes, PNUTL1 and PNUTL2. Gene<br />
2000;261(2):197-203<br />
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Bmf (CT)<br />
CATALOG No.: 3029<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development.<br />
Members in the Bcl-2 family are critical regulators of<br />
apoptosis by either inhibiting or promoting cell death. Bcl-2<br />
homology 3 (BH3) domain is a potent death domain. BH3-<br />
only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and<br />
PUMA, form a growing subclass of the Bcl-2 family. A novel<br />
BH3-only protein was recently identified in human and<br />
mouse and designated Bmf (for Bcl-2-modifing factor) (1).<br />
The BH3 domain in Bmf is required both for binding to Bcl-2<br />
proteins and for triggering apoptosis. In healthy cells, Bmf<br />
associates with the dynein light chain 2 (DLC2) component<br />
of the myosin V motors and is sequestered by the cell’s actin<br />
cytoskeleton. Disruption of the actin cytoskeleton, either by<br />
depolymerization of actin filaments or by detachment of cells<br />
from the extracellular matrix, triggers release and activation<br />
of Bmf, initiating the downstream apoptotic program (1,2).<br />
Bmf is constitutively expressed in many tissues (1,2).<br />
SOURCE:<br />
Rabbit anti-Bmf polyclonal antibody was raised with a<br />
synthetic peptide (ALNGEENRNGAGPR) corresponding to<br />
amino acids 171 to 184 of human Bmf (1).<br />
APPLICATION:<br />
This antibody can be used for detection of Bmf by Western<br />
blot at 2 µg/ml. HepG2 or 293 cell lysate can be used as a<br />
positive control and a band at approximately 25 kDa can be<br />
detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3029P).<br />
HepG2 (<strong>Catalog</strong> No. 1204) cell lysate, 200 µg at 2 mg/ml, is<br />
available for positive control<br />
REFERENCES:<br />
1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L,<br />
Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only<br />
protein regulated by interaction with the myosin V actin motor<br />
complex, activated by anoikis.<br />
Science. 2001;293(5536):1829-32.<br />
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science.<br />
2001;293(5536):1784-5.<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-Bmf (NT)<br />
CATALOG No.: 3031<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development.<br />
Members in the Bcl-2 family are critical regulators of<br />
apoptosis by either inhibiting or promoting cell death. Bcl-2<br />
homology 3 (BH3) domain is a potent death domain. BH3-<br />
only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and<br />
PUMA, form a growing subclass of the Bcl-2 family. A novel<br />
BH3-only protein was recently identified in human and<br />
mouse and designated Bmf (for Bcl-2-modifing factor) (1).<br />
The BH3 domain in Bmf is required both for binding to Bcl-2<br />
proteins and for triggering apoptosis. In healthy cells, Bmf<br />
associates with the dynein light chain 2 (DLC2) component<br />
of the myosin V motors and is sequestered by the cell’s actin<br />
cytoskeleton. Disruption of the actin cytoskeleton, either by<br />
depolymerization of actin filaments or by detachment of cells<br />
from the extracellular matrix, triggers release and activation<br />
of Bmf, initiating the downstream apoptotic program (1,2).<br />
Bmf is constitutively expressed in many tissues (1,2).<br />
SOURCE:<br />
Rabbit anti-Bmf polyclonal antibody was raised with a<br />
synthetic peptide (EPSQCVEELEDDV) corresponding to<br />
amino acids 2 to 14 of human Bmf (1). This sequence differs<br />
from that of mouse by one amino acid.<br />
APPLICATION:<br />
This antibody can be used for detection of Bmf by Western<br />
blot at 2 µg/ml. HepG2 or 293 cell lysate can be used as a<br />
positive control and a band at approximately 25 kDa can be<br />
detected. It is human and mouse reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of Bmf<br />
expression in human HepG2 (lane 1)<br />
and 293 (lane 2) cell lysates with anti-<br />
Bmf at 2 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3031P).<br />
HepG2 (<strong>Catalog</strong> No. 1204) cell lysate, 200 µg at 2 mg/ml, is<br />
available for positive control<br />
REFERENCES:<br />
1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L,<br />
Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only<br />
protein regulated by interaction with the myosin V actin motor<br />
complex, activated by anoikis.<br />
Science. 2001;293(5536):1829-32.<br />
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science.<br />
2001;293(5536):1784-5.<br />
(WD0204)
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Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Anti-EndoG<br />
CATALOG No.: 3035<br />
BACKGROUND:<br />
The fragmentation of nuclear DNA is a hallmark of apoptotic<br />
cell death. The activities of caspase and nuclease are<br />
involved in the DNA fragmentation. Caspase-activated<br />
deoxyribonuclease (CAD), also termed DNA fragmentation<br />
factor (DFF40), is one such nuclease, and is capable of<br />
inducing DNA fragmentation and chromatin condensation<br />
after cleavage by caspase-3 of its inhibitor ICAD/DFF45.<br />
Caspase and CAD independent DNA fragmentation also<br />
exists. Recent studies demonstrated that another nuclease,<br />
endonuclease G (endoG), is specifically activated by<br />
apoptotic stimuli and is able to induce nucleosomal<br />
fragmentation of DNA independently of caspase and<br />
DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease<br />
that translocates to the nucleus and cleaves chromatin DNA<br />
during apoptosis. The homologue of mammalian EndoG is<br />
the first mitochondrial protein identified to be involved in<br />
apoptosis in C. elegans (2). EndooG also cleaves DNA in<br />
vitro (4).<br />
SOURCE:<br />
Rabbit anti-EndoG polyclonal antibody was raised with a<br />
synthetic peptide (GGPRGPGELAKYGLP) corresponding to<br />
amino acids 55 to 70 of human EndoG (5).<br />
APPLICATION:<br />
This polyclonal antibody can be used for detection of EndoG<br />
by Western blot at 1 to 2 µg/ml. HepG2 and 3T3 cell lysates<br />
can be used as positive control and a 35 kDa band can be<br />
detected. It is human, mouse and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as affinity chromatography purified IgG, 100 µg<br />
in 100 µl of PBS containing 0.02% sodium azide. Store at<br />
4°C, stable for one year.<br />
Western blot analysis of EndoG in<br />
mouse (M) 3T3 and human (H) HepG2<br />
cell lysates with anti-EndoG at 2 µg/ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3035P).<br />
HepG2 cell lysate (<strong>Catalog</strong> No. 1211), 200 µg at 2 mg/ml, is<br />
available for positive control.<br />
REFERENCES:<br />
1. Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase<br />
when released from mitochondria. Nature. 2001;412(6842):95-9.<br />
2. Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial<br />
endonuclease G is important for apoptosis in C. elegans. Nature.<br />
2001;412(6842):90-4<br />
3. Hengartner MO. Apoptosis. DNA destroyers. Nature.<br />
2001;412(6842):27, 29.<br />
4. Widlak P, Li LY, Wang X, Garrard WT. Action of recombinant<br />
human apoptotic endonuclease G on naked DNA and chromatin<br />
substrates: cooperation with exonuclease and DNase I. J Biol<br />
Chem. 2001;276(51):48404-9.<br />
5. Tiranti V, Rossi E, Ruiz-Carrillo A, Rossi G, Rocchi M, DiDonato<br />
S, Zuffardi O, Zeviani M. Chromosomal localization of mitochondrial<br />
transcription factor A (TCF6), single-stranded DNA-binding protein<br />
(SSBP), and endonuclease G (ENDOG), three human<br />
housekeeping genes involved in mitochondrial biogenesis.<br />
Genomics. 1995;25(2):559-64. (WD0204)
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Anti-PUMA (CT) bbc3<br />
CATALOG No.: 3041<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development. The<br />
p53 tumor-suppressor protein induces apoptosis through<br />
transcriptional activation of several genes. A novel p53<br />
inducible pro-apoptotic gene was identified recently and<br />
designated PUMA (for p53 upregulated modulator of<br />
apoptosis) and bbc3 (for Bcl-2 binding component 3) in<br />
human and mouse (1-3). PUMA/bbc3 is one of the proapoptotic<br />
Bcl-2 family members including Bax and Noxa,<br />
which are also transcriptional targets of p53. The PUMA<br />
gene encodes two BH3 domain-containing proteins termed<br />
PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2,<br />
localize to the mitochondria, and induce cytochrome c<br />
release and apoptosis in response to p53. PUMA may be a<br />
direct mediator of p53-induced apoptosis.<br />
SOURCE:<br />
Rabbit anti-PUMA polyclonal antibody was raised against a<br />
synthetic peptide (PLPRGHRAPEMEPN) corresponding to<br />
amino acids 180 to 193 of human PUMA-α (1). This<br />
sequence is identical between α and β ????????the<br />
????????????.<br />
APPLICATION:<br />
This antibody can be used for detection of PUMA by<br />
Western blot at 1 to 2 µg/ml. K562 or 3T3 cell lysate can be<br />
used as a positive control and a band at approximately 23<br />
kDa can be detected. A lower band at approximately 16 kDa<br />
was detected in MOLT4 and U937 cells, which may<br />
represent the PUMA-β ????? It is human and mouse<br />
reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of PUMA<br />
expression in human (H) K562 and<br />
mouse (M) 3T3 cell lysates with anti-<br />
PUMA at 2 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3041P).<br />
K562 (<strong>Catalog</strong> No. 1204) or 3T3 (<strong>Catalog</strong> No. 1212) cell<br />
lysate, 200 µg at 2 mg/ml, is available for positive control<br />
REFERENCES:<br />
1. Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is<br />
induced by p53. Mol Cell. 2001;7(3):683-94.<br />
2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA<br />
induces the rapid apoptosis of colorectal cancer cells. Mol Cell.<br />
2001;7(3):673-82.<br />
3. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ,<br />
Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only<br />
gene, is regulated by diverse cell death and survival signals. Proc<br />
Natl Acad Sci U S A. 2001;98(20):11318-23.<br />
(WD0204)
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Anti-PUMA (NT) bbc3<br />
CATALOG No.: 3043<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development. The<br />
p53 tumor-suppressor protein induces apoptosis through<br />
transcriptional activation of several genes. A novel p53<br />
inducible pro-apoptotic gene was identified recently and<br />
designated PUMA (for p53 upregulated modulator of<br />
apoptosis) and bbc3 (for Bcl-2 binding component 3) in<br />
human and mouse (1-3). PUMA/bbc3 is one of the proapoptotic<br />
Bcl-2 family members including Bax and Noxa,<br />
which are also transcriptional targets of p53. The PUMA<br />
gene encodes two BH3 domain-containing proteins termed<br />
PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2,<br />
localize to the mitochondria, and induce cytochrome c<br />
release and apoptosis in response to p53. PUMA may be a<br />
direct mediator of p53-induced apoptosis.<br />
SOURCE:<br />
Rabbit anti-PUMA polyclonal antibody was raised with a<br />
synthetic peptide (ARARQEGSSPEPVEG) corresponding to<br />
amino acids 2 to 16 of human PUMA-α (1). This sequence is<br />
identical between human and mouse PUMA.<br />
APPLICATION:<br />
This antibody can be used for detection of PUMA by<br />
Western blot at 2 to 4 µg/ml. K562 cell lysate can be used<br />
as a positive control and a band at approximately 23 kDa<br />
can be detected.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of PUMA<br />
expression in human K562 cell lysate<br />
with anti-PUMA at 2 µg /ml.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3043P).<br />
K562 cell lysate (<strong>Catalog</strong> No. 1204), 200 µg at 2 mg/ml, is<br />
available for positive control.<br />
REFERENCES:<br />
1. Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is<br />
induced by p53. Mol Cell. 2001;7(3):683-94.<br />
2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA<br />
induces the rapid apoptosis of colorectal cancer cells. Mol Cell.<br />
2001;7(3):673-82.<br />
3. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ,<br />
Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only<br />
gene, is regulated by diverse cell death and survival signals. Proc<br />
Natl Acad Sci U S A. 2001;98(20):11318-23.<br />
(WD0204)
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techsupport@prosci-inc.com<br />
Anti-p53DINP1, SIP<br />
CATALOG No.: 3045<br />
BACKGROUND:<br />
Apoptosis is related to many diseases and development. The<br />
p53 tumor-suppressor protein induces apoptosis through<br />
transcriptional activation of several genes. A novel p53<br />
inducible gene was identified recently and designated<br />
p53DINP1 (for p53-dependent damage-inducible nuclear<br />
protein 1) and SIP (for stress induced protein) in human and<br />
mouse (1,2). A p53DINP1 antisense oligonucleotide inhibits<br />
and overexpression of p53DINP1 enhances Ser46<br />
phosphorylation of p53, induction of p53AIP1, and cell death<br />
induced by DNA double-strand breaks (1). p53DINP1 may<br />
regulate p53-dependent apoptosis through phosphorylation<br />
at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene<br />
encodes two proteins of 27 and 18 kDa in human and mouse<br />
termed p53DINP1-α and p53DINP1-β or SIP 27 and SIP 18<br />
(1,2). p53DINP1/SIP is expressed in many tissues and<br />
induced by a variety of stress agents including UV stress,<br />
mutagenic stress, heat shock, and oxidative stress (2).<br />
SOURCE:<br />
Rabbit anti-p53DINP1 polyclonal antibody was raised with a<br />
synthetic peptide (MFQRLNKMFVGEVS) corresponding to<br />
amino acids 1 to 14 of human p53DINP1 (1). This sequence<br />
is identical between α and β ????????the<br />
p53DINP1????????, and differs by one amino acid from<br />
those of mouse.<br />
APPLICATION:<br />
This antibody can be used for detection of p53DINP1 by<br />
Western blot at 1 to 2 µg/ml. Human lung tissue lysate can<br />
be used as a positive control and a band at 27 kDa can be<br />
detected. A lower band at 18 kDa was detected in human<br />
spleen, and mouse liver and kidney tissue lysates, which<br />
may represent the p53DINP1-β ????? It is human, mouse,<br />
and rat reactive.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as immunoaffinity chromatography purified IgG,<br />
100 µg in 100 µl of PBS containing 0.02% sodium azide.<br />
Store at 4°C, stable for one year.<br />
Western blot analysis of<br />
p53DINP1 expression in human<br />
lung tissue lysate with antip53DINP1<br />
at 2 µg/ml in the<br />
absence (lane 1) or presence<br />
(lane 2) of blocking peptide.<br />
RELATED PRODUCTS:<br />
Blocking peptide, 50 µg at 200 µg/ml, is available for<br />
competition studies (<strong>Catalog</strong> No. 3045P).<br />
Human lung tissue lysate (<strong>Catalog</strong> No. 1302), 200 µg at 2<br />
mg/ml, is available for positive control.<br />
REFERENCES:<br />
1. Okamura S, Arakawa H, Tanaka T, Nakanishi H, Ng CC, Taya Y,<br />
Monden M, Nakamura Y. p53DINP1, a p53-inducible gene,<br />
regulates p53-dependent apoptosis. Mol Cell. 2001;8(1):85-94.<br />
2. Tomasini R, Samir AA, Vaccaro MI, Pebusque MJ, Dagorn JC,<br />
Iovanna JL, Dusetti NJ. Molecular and functional characterization of<br />
the stress-induced protein (SIP) gene and its two transcripts<br />
generated by alternative splicing. SIP induced by stress and<br />
promotes cell death. J Biol Chem. 2001;276(47):44185-92.<br />
(WD0204)
peptide Data Sheets
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IRAK (CT) peptide<br />
CATALOG NO.: 1007P<br />
SEQUENCE: DRQGPEESDEFQS<br />
LOCATION: Amino acids 700 to 712 of human IRAK (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IRAK (CT, catalog number 1007). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-<br />
IRAK in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science<br />
1996;271:1128-31.
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CXCR4 (NT) peptide<br />
CATALOG NO.: 1009P<br />
SEQUENCE: MEGISIYTSDNYTE<br />
LOCATION: Amino acids 1 to 14 of human CXCR4 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CXCR4 (NT, catalog number 1009). Incubating<br />
the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of<br />
anti-CXCR4 (NT) in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Berson J.F. et al. J Virol 1996;70:6288-95.<br />
2. Leoetscher M. et al. J Biol Chem 1994;269:232-237
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CXCR4 (EL) peptide<br />
CATALOG NO.: 1012P<br />
SEQUENCE: DRYICDRFYPNDLWV<br />
LOCATION: Amino acids 182 to 196 of human CXCR4 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CXCR4 (EL, catalog number 1012). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-<br />
CXCR4 (EL) in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Berson J.F. et al. J Virol 1996;70:6288-95.<br />
2. Leoetscher M. et al. J Biol Chem 1994;269:232-237
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
CCR5 (NT) peptide<br />
CATALOG NO.: 1112P<br />
SEQUENCE: SSPIYDINYYTSEPC<br />
LOCATION: Amino acids 6 to 20 of human CCR5 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CCR5 (catalog number 1112). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-<br />
CCR5 in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Raport, CJ, et al. J Biol Chem 1996;271:17161-6<br />
2. Combadiere, C. et al. J Leuk Biol 1996;60:147-52
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
TRAIL (CT) peptide<br />
CATALOG NO.: 1113P<br />
SEQUENCE: CTNEHLIDMDHEASFFGA<br />
LOCATION: Amino acids 261 to 277 of human TRAIL (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-TRAIL (catalog number 1113). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-<br />
TRAIL (CT) in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch<br />
C, Smith CA, et al. Identification and characterization of a new member of the TNF family that<br />
induces apoptosis. Immunity 1995;3:673-682<br />
2. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. Induction of apoptosis by<br />
Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem.<br />
1996;271:12687-90
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Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
RAIDD (IN) peptide<br />
CATALOG NO.: 1115P<br />
SEQUENCE: CAGDRLTGIPSHILNSSPSD<br />
LOCATION: Amino acids 99 to 117 of human RAIDD (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-RAIDD (catalog number 1115). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-<br />
RAIDD (IN) in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89<br />
2. Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G, Fernandes-Alnemri T, Alnemri ES.<br />
CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor<br />
receptor-interacting protein RIP. Cancer Res 1997
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
RAIDD (CT) peptide<br />
CATALOG NO.: 1117P<br />
SEQUENCE: CHNGLRAVEVDPSLLLHMLE<br />
LOCATION: Amino acids 181 to 199 of human RAIDD (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-RAIDD (catalog number 1117). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89<br />
2. Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G, Fernandes-Alnemri T, Alnemri ES.<br />
CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor<br />
receptor-interacting protein RIP. Cancer Res 1997 57:615-619
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DR3 (CT) peptide<br />
CATALOG NO.: 1120P<br />
SEQUENCE: ERMGLDGCVEDLRSRLQRGP<br />
LOCATION: Amino acids 398 to 417 of human DR3 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR3 (catalog number 1120). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit<br />
VM. Science, 1996;274:990-2.<br />
2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN.<br />
Nature, 1996;384:372-5.
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techsupport@prosci-inc.com<br />
Neurturin peptide<br />
CATALOG NO.: 1121P<br />
SEQUENCE: DAHSRYHTVHELSARE<br />
LOCATION: Amino acids 178 to 193 of human neurturin (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-neurturin (catalog number 1121). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Kotzbauer PT, Lampe PA, Heuckeroth RO, Golden JP, Creedon DJ, Johnson EM Jr, lbrandt J.<br />
Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
Eotaxin peptide<br />
CATALOG NO.: 1123P<br />
SEQUENCE: NH 2 -DSMKYLDQKSPTPKP-OH<br />
LOCATION: Amino acids 83 to 97 of human eotaxin (1-2).<br />
Application:<br />
The peptide is used for blocking the antibody activity of eotaxin (catalog number 1123). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective<br />
CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J<br />
Biol Chem 1996;271:7725-30<br />
2. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin.<br />
Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment<br />
of eosinophils. J Clin Invest 1996;97:604-12
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techsupport@prosci-inc.com<br />
SIRPα (CT) peptide<br />
CATALOG NO.: 1125P<br />
SEQUENCE: KPEPSFSEYASVQVPRK<br />
LOCATION: Amino acids 487 to 503 of human SIRPα (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-SIRPα (catalog number 1125). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Kharitonenkov A, et al. Nature 1997;386:181-186.
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techsupport@prosci-inc.com<br />
Caspase-10 (CT) peptide<br />
CATALOG NO.: 1128P<br />
SEQUENCE: ISAQTPRPPMRRWSSVS<br />
LOCATION: Amino acids 505 to 521 of human caspase-10 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-caspase-10 (catalog number 1128). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Vincenz.C. and Dixit,V.M. Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2),<br />
an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem.<br />
1997;272:6578-6583
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Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
NIK (CT) peptide<br />
CATALOG NO.: 1129P<br />
SEQUENCE: SFAWSWRVKHGQLENRP<br />
LOCATION: Amino acids 931 to 947 of human NIK (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-NIK (catalog number 1129). Incubating the peptide<br />
with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in<br />
Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3K-related kinase involved in NF-kappaB<br />
induction by TNF, CD95 and IL-1. Nature 1997;385:540-544
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techsupport@prosci-inc.com<br />
TACE (CT) peptide<br />
CATALOG NO.: 1131P<br />
SEQUENCE: ASFKLQRQNRVDSKETE<br />
LOCATION: Amino acids 807 to 823 of human TACE (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-TACE (CT, catalog number 1131). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the activity of anti-<br />
TACE in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCES:<br />
1. Black RA, Rauch CT, Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumournecrosis<br />
factor-alpha from cells. Nature 1997;385:729-733<br />
2. Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor<br />
tumour-necrosis factor-alpha. Nature 1997;385:733-736
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
GFRα-1 (IN) peptide<br />
CATALOG NO.: 1133P<br />
SEQUENCE: KNKPLGPAGSENEI<br />
LOCATION: Amino acids 369 to 382 of human GFRα-1 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-GFRα-1 (catalog number 1133). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z; Cupples R; Louis JC;<br />
Hu S; Altrock BW; Fox GM. GDNF-induced activation of the Ret protein tyrosien kinase is mediated<br />
by GDNFR-? , a novel receptor for GDNF. Cell, 1996;85:1113-24.<br />
2. Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD; Gray C; Armanini MP;<br />
Pollock RA; Herti F; Phillips HS; Goddard A; Moore MW; Buj-Bello A;Davies AM; Asai N;<br />
Takahashi M; Vandlen R; Henderson CE; Rosenthal A. Characterization of a multicomponent receptor<br />
for GDNF. Nature, 1996;382:80-83.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
GFRα-3 (IN) peptide<br />
CATALOG NO.: 1137P<br />
SEQUENCE: RFHRQLFSQDWADS<br />
LOCATION: Amino acids 347 to 360 of murine GFRα-3 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-GFRα-3 (catalog number 1137). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Naveilhan P, Baudet C, Mikaels A, Shen L, Westphal H, Ernfors P. Expression and regulation of<br />
GFRalpha3, a glial cell line-derived neurotrophic factor family receptor. Proc Natl Acad Sci USA<br />
1998;95:1295-1300.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DR4 (CT) peptide<br />
CATALOG NO.: 1139P<br />
SEQUENCE: CDSGKFIYLEDGTGSAVSLE<br />
LOCATION: Amino acids 427 to 445 of human DR4 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR4 (catalog number 1139). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor<br />
for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DFF45 (CT) peptide<br />
CATALOG NO.: 1141P<br />
SEQUENCE: KASPPGDLQNPKRARQDPT<br />
LOCATION: Amino acids 313 to 331 of human DFF45 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DFF45 (catalog number 1141). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of<br />
caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184
13100 Kirkham Way<br />
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www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DFF45/35 (NT) peptide<br />
CATALOG NO.: 1148P<br />
SEQUENCE: EVTGDAGVPESGEIRTLKPC<br />
LOCATION: Amino acids 2 to 21 of human DFF45/35 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DFF45/35 (catalog number 1148). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of<br />
caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
MADD (NT) peptide<br />
CATALOG NO.: 1150P<br />
SEQUENCE: AAVHSSEEDLRTPPRPVSS<br />
LOCATION: Amino acids 1570 to 1588 of human MADD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-MADD (catalog number 1150). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Schievella AR, Chen JH, Graham JR, Lin LL. MADD, a novel death domain protein that interacts with<br />
the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J Biol Chem<br />
1997;272:12069-12075
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
mFLIP (CT) peptide<br />
CATALOG NO.: 1156P<br />
SEQUENCE: DKVYAWNSGVSSKEKYS<br />
LOCATION: Amino acids 449 to 465 of human mFLIP (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-mFLIP (catalog number 1156). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K,<br />
Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP.<br />
Nature 1997;388:190-195.<br />
2. Goltsev YV, Kovalenko AV, Arnold E, Varfolomeev EE, Brodianskii VM, Wallach D. CASH, a<br />
novel caspase homologue with death effector domains. J Biol Chem 1997;272:19641-19644.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DR3 (ED) peptide<br />
CATALOG NO.: 1158P<br />
SEQUENCE: APCTEPCGNSTCLVCPQDT<br />
LOCATION: Amino acids 59 to 77 of human DR3 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR3 (catalog number 1158). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit<br />
VM. Science, 1996;274:990-2.<br />
2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN.<br />
Nature, 1996;384:372-5.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
FLIP (NT) peptide<br />
CATALOG NO.: 1159P<br />
SEQUENCE: SAEVIHQVEEALDTDEKE<br />
LOCATION: Amino acids 2 to 18 of human FLIP (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-FLIP (catalog number 1159). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K,<br />
Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP.<br />
Nature 1997;388:190-195
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
FLIPα (CT) peptide<br />
CATALOG NO.: 1161P<br />
SEQUENCE: NGYMYDWNSRVSAKEKYY<br />
LOCATION: Amino acids 447 to 464 of human FLIP? (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-FLIP? (catalog number 1161). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K,<br />
Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP.<br />
Nature 1997;388:190-195
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Daxx (CT) peptide<br />
CATALOG NO.: 1163P<br />
SEQUENCE: TSVATQCDPEEIIVLSDSD<br />
LOCATION: Amino acids 722 to 740 of human Daxx (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-Daxx (catalog number 1163). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel Fas-binding protein that activates<br />
JNK and apoptosis. Cell 1997;89:1067-1076<br />
2. Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd. Cloning and expression of primate Daxx<br />
cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol<br />
1997;16:1289-1298 5.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DR3 (NT) peptide<br />
CATALOG NO.: 1166P<br />
SEQUENCE: QGGTRSPRCDCAGDFH<br />
LOCATION: Amino acids 25 to 40 of human DR3 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR3 (catalog number 1166). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Chinnaiyan AM; O'Rourke K; Yu GL; Lyons RH; Garg M; Duan DR; Xing L; Gentz R; Ni J; Dixit<br />
VM. Science, 1996;274:990-2.<br />
2. Kitson J; Raven T; Jiang YP; Goeddel DV; Giles KM; Pun KT; Grinham CJ; Brown R; Farrow SN.<br />
Nature, 1996;384:372-5.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DR4 (NT) peptide<br />
CATALOG NO.: 1167P<br />
SEQUENCE: ASGTEAAAATPSKVWGSSAG<br />
LOCATION: Amino acids 1 to 20 of human DR4 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR4 (catalog number 1167). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor<br />
for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Bonzo (CT) peptide<br />
CATALOG NO.: 1170P<br />
SEQUENCE: KSSEDNSKTFSASHNVEATS<br />
LOCATION: Amino acids 319 to 338 of human Bonzo (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-Bonzo (catalog number 1170). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by<br />
simian and human immunodeficiency viruses. Nature 1997;388:296-300<br />
2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine<br />
receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic<br />
HIV-1. J Exp Med 1997;185:2015-23.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
ICAD (NT) peptide<br />
CATALOG NO.: 2001P<br />
SEQUENCE: ELSRGASAPDPDDVRPLKPC<br />
LOCATION: Amino acids 2 to 21 of murine ICAD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-ICAD (catalog number 2001). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that<br />
degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
ICAD (CT) peptide<br />
CATALOG NO.: 2003P<br />
SEQUENCE: -RRSPLPGEPQRPKRAKRDSS<br />
LOCATION: Amino acids 312 to 331 of murine ICAD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-ICAD (catalog number 2003). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that<br />
degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CAD (I17) peptide<br />
CATALOG NO.: 2007P<br />
SEQUENCE: YNGSYFDRGAEASSRL<br />
LOCATION: Amino acids 205 to 222 of murine CAD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CAD (catalog number 2007). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that<br />
degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CAD (CT) peptide<br />
CATALOG NO.: 2011P<br />
SEQUENCE: KLECDRSRIYRPQTGS<br />
LOCATION: Amino acids 314 to 329 of murine CAD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CAD (catalog number 2011). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that<br />
degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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techsupport@prosci-inc.com<br />
Apaf-1 (NT) peptide<br />
CATALOG NO.: 2013P<br />
SEQUENCE: HREALEKDIKTSYIMDHC<br />
LOCATION: Amino acids 12 to 28 of human Apaf-1 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-Apaf-1 (catalog number 2013). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans<br />
CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
Apaf-1 (CT) peptide<br />
CATALOG NO.: 2015P<br />
SEQUENCE: TFYTNGTNLKKIHVSPDFKT<br />
LOCATION: Amino acids 1158 to 1177 of human Apaf-1 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-Apaf-1 (catalog number 2015). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans<br />
CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DR5 (CT) peptide<br />
CATALOG NO.: 2019P<br />
SEQUENCE: KIEDHLLSSGKFMYLEGNAD<br />
LOCATION: Amino acids 388 to 407 of human DR5 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR5 (catalog number 2019). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domaincontaining<br />
receptor for TRAIL. Science 1997;277:815-8<br />
2. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL,<br />
Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by<br />
a family of signaling and decoy receptors. Science 1997;277:818-21
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DcR2 (ID) peptide<br />
CATALOG NO.: 2021P<br />
SEQUENCE: GGPERVHRVLFRRRS<br />
LOCATION: Amino acids 249 to 263 of human DcR2 (1-3)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DcR2 (catalog number 2021). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor<br />
for the cytotoxic ligand TRAIL. Science; 1997;276:111-113<br />
2. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domaincontaining<br />
receptor for TRAIL. Science 1997;277:815-8<br />
3. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL,<br />
Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by<br />
a family of signaling and decoy receptors. Science 1997;277:818-21
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IKKα (C1) peptide<br />
CATALOG NO.: 2025P<br />
SEQUENCE: CLGHLSTIIHEANEEQGNS<br />
LOCATION: Amino acids 716 to 734 of human IKKα (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IKKα (catalog number 2025). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase<br />
that activates the transcription factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an<br />
IkappaB kinase. Cell 1997;90:373-83
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
ADAM10 (CT) peptide<br />
CATALOG NO.: 2051P<br />
SEQUENCE: PQRQRPRESYQMGHMRR<br />
LOCATION: Amino acids 732 to 748 of human ADAM10 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-ADAM10 (catalog number 2051). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumor necrosis<br />
factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem<br />
1997;272:24588-93
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
FLIPs (CT) peptide<br />
CATALOG NO.: 2055P<br />
SEQUENCE: QKSLKDPSNNFRMITPYAH<br />
LOCATION: Amino acids 191 to 209of human FLIPs (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-FLIPs (catalog number 2055). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K,<br />
Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP.<br />
Nature 1997;388:190-195
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DNase II (CT) peptide<br />
CATALOG NO.: 2059P<br />
SEQUENCE: NH 2 -CNGMARKPSRAYKI-OH<br />
LOCATION: Amino acids 347 to 360 of human DNase II precursor (1-3).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of DNase II (catalog number 2059). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning<br />
of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6<br />
2. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role<br />
in apoptosis. J Biol Chem 1998;273:30909-14<br />
3. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem<br />
Biophys Res Commun 1998;247:864-9
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Bim (IN) peptide<br />
CATALOG NO.: 2065P<br />
SEQUENCE: AERPPQLRPGAPTSLQTEP<br />
LOCATION: Amino acids 22 to 40 of human Bim (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-Bim (catalog number 2065). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. O'Connor,L., Strasser,A., O'Reilly,L.A., Hausmann,G., Adams,J.M., Cory,S. and Huang,D.C. Bim: a<br />
novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998;17:384-395
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
ZIPK (IN) peptide<br />
CATALOG NO.: 2067P<br />
SEQUENCE: RRRNVRGEDSGRKPERRRLK<br />
LOCATION: Amino acids 279 to 298 of human ZIPK (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-ZIPK (catalog number 2067). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which<br />
mediates apoptosis. Mol Cell Biol 1998;18:1642-51
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Caspase-9 (IN1) peptide<br />
CATALOG NO.: 2071P<br />
SEQUENCE: EDIQRAGSGSRRDQAR<br />
LOCATION: Amino acids 41 to 56 of human caspase-9 (1-3)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-caspase-9 (catalog number 2071). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel<br />
member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol<br />
Chem 1996;271:16720-4<br />
2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA,<br />
Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog<br />
Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J<br />
Biol Chem 1996;271:27099-106<br />
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and<br />
dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell<br />
1997;91:479-89
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Caspase-9 (IN2) peptide<br />
CATALOG NO.: 2073P<br />
SEQUENCE: ASTSPEDESPGSNPEPDATP<br />
LOCATION: Amino acids 299 to 318 of human caspase-9 (1-3)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-caspase-9 (catalog number 2073). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel<br />
member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol<br />
Chem 1996;271:16720-4<br />
2. Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA,<br />
Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog<br />
Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J<br />
Biol Chem 1996;271:27099-106<br />
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and<br />
dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell<br />
1997;91:479-89
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
RICK (NT) peptide<br />
CATALOG NO.: 2075P<br />
SEQUENCE: PTIPYHKLADLRYLSRGASG<br />
LOCATION: Amino acids 11 to 30 of human RICK (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-RICK (catalog number 2075). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase<br />
recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem<br />
1998;273:12296-300
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
ARC (CT) peptide<br />
CATALOG NO.: 2081P<br />
SEQUENCE: PDPEPEPDFEERDESEDS<br />
LOCATION: Amino acids 191 to 208 of human ARC (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-ARC (catalog number 2081). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle<br />
and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CIDE-A (CT) peptide<br />
CATALOG NO.: 2085P<br />
SEQUENCE: DDKEERPSLRSQAKGRFT<br />
LOCATION: Amino acids 200 to 217 of human CIDE-A (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CIDE-A (catalog number 2085). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with<br />
homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
mCIDE-A (CT) peptide<br />
CATALOG NO.: 2089P<br />
SEQUENCE: GDTEEQPSPKPSTKG<br />
LOCATION: Amino acids 200 to 214 of murine CIDE-A (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-mCIDE-A (catalog number 2089). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with<br />
homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
mCIDE-B (CT) peptide<br />
CATALOG NO.: 2091P<br />
SEQUENCE: EGADRWQWHGQRHLHS<br />
LOCATION: Amino acids 204 to 219 of murine CIDE-B (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-mCIDE-B (catalog number 2091). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with<br />
homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CX 3 CR1 (NT) peptide<br />
CATALOG NO.: 2093P<br />
SEQUENCE: DQFPESVTENFEYDDLAEAC<br />
LOCATION: Amino acids 2 to 21 of murine CX 3 CR1 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CX 3 CR1 (catalog number 2093). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The orphan G-protein-coupled<br />
receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in<br />
lymphoid and neural tissues. Gene 1995;163:295-9<br />
2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization, and RNA expression of a<br />
human beta chemokine receptor-like gene. DNA Cell Biol 1995;14:673-80
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CCR8 (EL) peptide<br />
CATALOG NO.: 2097P<br />
SEQUENCE: CYSFYNQQTLKWKIFTNFK<br />
LOCATION: Amino acids 732 to 748 of human CCR8 (1-3)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CCR8 (catalog number 2097). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A, Storlazzi CT, Rocchi M, Santoni A.<br />
Molecular cloning of TER1, a chemokine receptor-like gene expressed by lymphoid tissues. J Immunol<br />
1996;157:2759-63<br />
2. Zaballos A, Varona R, Gutierrez J, Lind P, Marquez G. Molecular cloning and RNA expression of two<br />
new human chemokine receptor-like genes. Biochem Biophys Res Commun 1996;227:846-53<br />
3. Samson M, Stordeur P, Labbe O, Soularue P, Vassart G, Parmentier M. Molecular cloning and<br />
chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and<br />
polymorphonuclear cells and encoding a putative chemokine receptor. Eur J Immunol 1996;26:3021-8
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
GPR15 (NT) peptide<br />
CATALOG NO.: 2101P<br />
SEQUENCE: YYATSPNSDIRETHSH<br />
LOCATION: Amino acids 13 to 28 of murine GPR15 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-GPR15 (catalog number 2101). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Heiber M, Marchese A, Nguyen T, Heng HH, George SR, O'Dowd BF. A novel human gene encoding<br />
a G-protein-coupled receptor (GPR15) is located on chromosome 3. Genomics 1996;32:462-5
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DFF40/CAD (I18) peptide<br />
CATALOG NO.: 2107P<br />
SEQUENCE: EGLESRFRNKSGYLRYS<br />
LOCATION: Amino acids 147 to 164 of murine CAD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DFF40/CAD (catalog number 2107). Incubating<br />
the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody<br />
activity in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that<br />
degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IKKα (C2) peptide<br />
CATALOG NO.: 2115P<br />
SEQUENCE: TPQDGETSAQMIEENLN<br />
LOCATION: Amino acids 699 to 715 of human IKKα (1-3)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IKKα (catalog number 2115). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase<br />
that activates the transcription factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an<br />
IkappaB kinase. Cell 1997;90:373-83
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IKKα (C3) peptide<br />
CATALOG NO.: 2117P<br />
SEQUENCE: CTQSSARSLVGSSLEGA<br />
LOCATION: Amino acids 658 to 674 of human IKKα (1-3)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IKKα (catalog number 2117). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase<br />
that activates the transcription factor NF-kappaB. Nature 1997;388:548-54<br />
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an<br />
IkappaB kinase. Cell 1997;90:373-83
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IKKβ (C3) peptide<br />
CATALOG NO.: 2121P<br />
SEQUENCE: CSKVRGPVSGSPDSMNASR<br />
LOCATION: Amino acids 662 to 680 of human IKKβ (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IKKβ (catalog number 2121). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IkappaB kinase complex (IKK)<br />
contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NFkappaB<br />
activation. Cell 1997;91:243-52<br />
2. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB kinase-beta: NF-kappaB activation and<br />
complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-9
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IRAK2 (C2) peptide<br />
CATALOG NO.: 2123P<br />
SEQUENCE: APWAGAATPLLPTENGEGR<br />
LOCATION: Amino acids 546 to 564 of human IRAK2 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IRAK2 (catalog number 2123). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal<br />
mediators of IL-1 signaling. Science 1997;278:1612-5
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
MyD88 (IN) peptide<br />
CATALOG NO.: 2125P<br />
SEQUENCE: CDFQTKFALSLSPGAHD<br />
LOCATION: Amino acids 233 to 248 of human MyD88 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-MyD88 (catalog number 2125). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE. The cloning and characterization of<br />
human MyD88: amember of an IL-1 receptor related family. FEBS Lett 1997;402:81-4
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
MyD88 (CT) peptide<br />
CATALOG NO.: 2127P<br />
SEQUENCE: CTKSWFWTRLAKALSLP<br />
LOCATION: Amino acids 279 to 296 of human MyD88 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-MyD88 (catalog number 2127). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, Sims JE. The cloning and characterization of<br />
human MyD88: a member of an IL-1 receptor related family. FEBS Lett 1997;402:81-4
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-1RAcP (C2) peptide<br />
CATALOG NO.: 2129P<br />
SEQUENCE: KWKGEKSKYPQGRFWK<br />
LOCATION: Amino acids 525 to 540 of human IL-1RAcP (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IL-1RAcP (catalog number 2129). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G. Molecular cloning and<br />
characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem<br />
1995;270:13757-65
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-1RAcP (CT) peptide<br />
CATALOG NO.: 2131P<br />
SEQUENCE: KKSPRRSSSDEQGLSYSSLKN<br />
LOCATION: Amino acids 549 to 569 of human IL-1RAcP (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IL-1RAcP (catalog number 2131). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Greenfeder SA, Nunes P, Kwee L, Labow M, Chizzonite RA, Ju G. Molecular cloning and<br />
characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem<br />
1995;270:13757-65
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
APP (AβNT) peptide<br />
CATALOG NO.: 2133P<br />
SEQUENCE: DAEFRHDSGY<br />
LOCATION: Amino acids 653 to 662 of human APP (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-APP (catalog number 2133). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W,<br />
Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase<br />
inhibitors. Nature 1988;331:525-7.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
APP (CT) peptide<br />
CATALOG NO.: 2136P<br />
SEQUENCE: GYENPTYKFFEQMQN<br />
LOCATION: Amino acids 737 to 751 of human APP (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-APP (catalog number 2136). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W,<br />
Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase<br />
inhibitors. Nature 1988;331:525-7.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DcR3 (NT) peptide<br />
CATALOG NO.: 2140P<br />
SEQUENCE: AETPTYPWRDAETGERC<br />
LOCATION: Amino acids 31 to 46 of human DcR3 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DcR3 (catalog number 2140). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, Huang A, Donahue CJ, Sherwood SW,<br />
Baldwin DT, Godowski PJ, Wood WI, Gurney AL, Hillan KJ, Cohen RL, Goddard AD, Botstein D, Ashkenazi A.<br />
Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 1998;396:699-703<br />
2. Yu KY, Kwon B, Ni J, Zhai Y, Ebner R, Kwon BS. A newly identified member of tumor necrosis factor receptor<br />
superfamily (TR6) suppresses LIGHT-mediated apoptosis. J Biol Chem 1999;274:13733-6
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
SODD (NT) peptide<br />
CATALOG NO.: 2143P<br />
SEQUENCE: SALRRSGYGPSDGPSC<br />
LOCATION: Amino acids 2 to 16 of human SODD (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-SODD (catalog number 2143). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of constitutive TNF receptor 1 signaling by<br />
silencer of death domains. Science 1999;283:543-6
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DRAK1 (NT) peptide<br />
CATALOG NO.: 2147P<br />
SEQUENCE: EKPGSGGSSPGATSGC<br />
LOCATION: Amino acids 5 to 19 of human DRAK1 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DRAK1 (catalog number 2147). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein<br />
kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DRAK2 (CT) peptide<br />
CATALOG NO.: 2149P<br />
SEQUENCE: CSKRFRFDDSLPNPHE<br />
LOCATION: Amino acids 351 to 365 of human DRAK2 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DRAK2 (catalog number 2149). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein<br />
kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DFF40 (NT) peptide<br />
CATALOG NO.: 2153P<br />
SEQUENCE: QKPKSVKLRALRSPRKC<br />
LOCATION: Amino acids 3 to 18 of human DFF40 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DFF40 (catalog number 2153). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA<br />
fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
2. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H, Nagata S. Molecular cloning and<br />
characterization of human caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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DFF40 (IN) peptide<br />
CATALOG NO.: 2155P<br />
SEQUENCE: NGSYFDRGAKGGSRLC<br />
LOCATION: Amino acids 203 to 218 of human DFF40 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DFF40 (catalog number 2155). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA<br />
fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc<br />
Natl Acad Sci USA 1998;95:8461-6<br />
2. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H, Nagata S. Molecular cloning and<br />
characterization of human caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8
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techsupport@prosci-inc.com<br />
DR6 (NT) peptide<br />
CATALOG NO.: 2157P<br />
SEQUENCE: QPEQKASNLIGTYRHC<br />
LOCATION: Amino acids 42 to 56 of human DR6 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DR6 (catalog number 2157). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pan G, Bauer JH, Haridas V, Wang S, Liu D, Yu G, Vincenz C, Aggarwal BB, Ni J, Dixit VM.<br />
Identification and functional characterization of DR6, a novel death domain-containing TNF receptor.<br />
FEBS Lett 199;431:351-6
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techsupport@prosci-inc.com<br />
BCL-10 (NT) peptide<br />
CATALOG NO.: 2161P<br />
SEQUENCE: APSLTEEDLTEVKKDC<br />
LOCATION: Amino acids 5 to 19 of human BCL-10 (1-5)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-BCL-10 (catalog number 2161). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and<br />
mutated in multiple tumor types. Cell 1999;96(1):35-45<br />
2. Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF kappaB-activating protein containing a caspase<br />
recruitment domain with homology to Herpesvirus-2 protein E10. J Biol Chem 1999;274(15):9955-61<br />
3. Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel caspase recruitment domain-containing<br />
proapoptotic molecule. J Biol Chem 1999;274(15):10287-92<br />
4. Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2 E10 gene product, but not its cellular homologue,<br />
activates NF-kappaB transcription factor and c-Jun N-terminal kinase. J Biol Chem 1999;274(15):9962-8<br />
5. Srinivasula SM, Ahmad M, Lin JH, et al. CLAP, a novel caspase recruitment domain-containing protein in the<br />
tumor necrosis factor receptor pathway, regulates NF-kappaB activation and apoptosis. J Biol Chem<br />
1999;274(25):17946-54
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techsupport@prosci-inc.com<br />
DcR1 (ED) peptide<br />
CATALOG NO.: 2179P<br />
SEQUENCE: CVEEFGANATVETPAAEET<br />
LOCATION: Amino acids 149 to 167 of human DcR1 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-DcR1 (catalog number 2179). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year..<br />
REFERENCE:<br />
1. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for<br />
TRAIL. Science 1997;277:815-8<br />
2. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of<br />
signaling and decoy receptors. Science 1997;277:818-21
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techsupport@prosci-inc.com<br />
RICK (CT) peptide<br />
CATALOG NO.: 2183P<br />
SEQUENCE: KLKDNKQMGLQPYPE<br />
LOCATION: Amino acids 508 to 522 of human RICK (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-RICK (catalog number 2183). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase<br />
recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem<br />
1998;273:12296-300
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techsupport@prosci-inc.com<br />
ARC (NT) peptide<br />
CATALOG NO.: 2185P<br />
SEQUENCE: GNAQERPSETIDRERKR<br />
LOCATION: Amino acids 2 to 18 of human ARC (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-ARC (catalog number 2185). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle<br />
and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60
13100 Kirkham Way<br />
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techsupport@prosci-inc.com<br />
CX 3 CR1 (EL) peptide<br />
CATALOG NO.: 2201P<br />
SEQUENCE: CLGDYPEVLQEIWPV<br />
LOCATION: Amino acids 175 to 189 of human CX 3 CR1/V28 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-CX 3 CR1 (catalog number 2201). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The orphan G-protein-coupled<br />
receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in<br />
lymphoid and neural tissues. Gene 1995;163:295-9<br />
2. Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization, and RNA expression of a<br />
human beta chemokine receptor-like gene. DNA Cell Biol 1995;14:673-80
13100 Kirkham Way<br />
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techsupport@prosci-inc.com<br />
Bonzo (NT2) peptide<br />
CATALOG NO.: 2209P<br />
SEQUENCE: GFSSFNDSSQEEHQD<br />
LOCATION: Amino acids 11 to 25 of human Bonzo/STRL33 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-Bonzo (catalog number 2209). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by<br />
simian and human immunodeficiency viruses. Nature 1997;388:296-300<br />
2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine<br />
receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic<br />
HIV-1. J Exp Med 1997;185:2015-23
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IRAK2 (CT) peptide<br />
CATALOG NO.: 2213P<br />
SEQUENCE: READSSSEACVGLEPPQDVT<br />
LOCATION: Amino acids 571 to 590 of human IRAK2 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of anti-IRAK2 (catalog number 2213). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal<br />
mediators of IL-1 signaling. Science 1997;278:1612-5
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Acinus (CP) peptide<br />
CATALOG NO.: 2215P<br />
SEQUENCE: NH 2 -TRTALHGVKWPQSNPK-OH<br />
LOCATION: Amino acids 1065 to 1080 of human AcinusL, 338 to 353 of human AcinusS’, or 307 to 322<br />
of human AcinusS, which are identical to those of mouse Acinus (1). The selected antigenic sequence is<br />
located near the C-terminus of the cleaved active peptide p17.<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of Acinus (catalog number 2215). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated<br />
protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Acinus (CT) peptide<br />
CATALOG NO.: 2217P<br />
SEQUENCE: NH 2 -SRSTPVRDRGGRR-OH<br />
LOCATION: Amino acids 1329 to 1341 of human AcinusL, 602 to 614 of human AcinusS’, or 571 to 583<br />
of human AcinusS (1).<br />
Application:<br />
The peptide is used for blocking the antibody activity of Acinus (catalog number 2217). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated<br />
protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
Acinus (IN) peptide<br />
CATALOG NO.: 2219P<br />
SEQUENCE: NH 2 -DTSENRPENDVPEPP-OH<br />
LOCATION: Amino acids 775 to 789 of human AcinusL, 48 to 62 of human AcinusS’, or 17 to 31 of<br />
human AcinusS (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of Acinus (catalog number 2219). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated<br />
protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
BAFF (CT) peptide<br />
CATALOG NO.: 2221P<br />
SEQUENCE: NH 2 -EEGDELQLAIPRENAQ-OH<br />
LOCATION: Amino acids 254 to 269 of human BAFF/BLys (1,4)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of BAFF (catalog number 2221). It usually blocks the<br />
antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for<br />
30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B<br />
lymphocyte stimulator. Science 1999;285:260-3<br />
2. Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family,<br />
stimulates B cell growth. J Exp Med 1999;189:1747-56<br />
3. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by<br />
mitogens. J Leukoc Biol 1999;65:680-3<br />
4. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. Identification and characterization of a novel<br />
cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-<br />
terminal kinase. J Biol Chem 1999 ;274:15978-81
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
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techsupport@prosci-inc.com<br />
CATALOG NO.: 2223P<br />
SEQUENCE: NH 2 -GTGGPSQNGEGYP-OH<br />
APRIL (ED) (TALL-2/TRDL-1) peptide<br />
LOCATION: Amino acids 67 to 79 of human APRIL (1-3).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of APRIL (catalog number 2423). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor<br />
family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.<br />
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is downregulated<br />
by mitogens. J Leukoc Biol. 1999;65(5):680-3.<br />
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand,<br />
stimulates cell death. Cancer Res. 2000;60(4):1021-7.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DEDAF peptide<br />
CATALOG NO.: 2227P<br />
SEQUENCE: NH 2 -TPKGDMSAVNDESF-OH<br />
LOCATION: Amino acids 215 to 228 of human DADEF (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of DEDAF (catalog number 2227). It usually blocks the<br />
antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for<br />
30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Zheng L, Schickling O, Peter ME, Lenardo MJ. The death effector domain-associated factor plays<br />
distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 2001;276(34):31945-52.
13100 Kirkham Way<br />
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www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Survivin (NT) peptide<br />
CATALOG NO.: 2233P<br />
SEQUENCE: NH 2 -MGAPTLPPAWQP-OH<br />
LOCATION: Amino acids 1 to 12 of human survivin (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of survivin (catalog number 2233). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and<br />
lymphoma. Nat Med 1997;3:917-21
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Survivin (CT) peptide<br />
CATALOG NO.: 2235P<br />
SEQUENCE: NH 2 -KKVRRAIEQLAAMD-OH<br />
LOCATION: Amino acids 129 to 142 of human survivin (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of survivin (catalog number 2235). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and<br />
lymphoma. Nat Med 1997;3:917-21
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Survivin (CT) peptide<br />
CATALOG NO.: 2237P<br />
SEQUENCE: NH 2 -AKTTRQSIEQLAA-OH<br />
LOCATION: Amino acids 128 to 140 of mouse survivin (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of survivin (catalog number 2237). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T. Expression of a murine homologue<br />
of the inhibitor of apoptosis protein is related to cell proliferation. Proc Natl Acad Sci USA 1999;96:1457-<br />
62
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AIF (NT) peptide<br />
CATALOG NO.: 2239P<br />
SEQUENCE: NH 2 -KQKKAALSASEGEE-OH<br />
LOCATION: Amino acids 109 to 122 of human AIF (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of AIF (catalog number 2239). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosisinducing<br />
factor. Nature 1999;397:441-6
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techsupport@prosci-inc.com<br />
Acinus (NP) peptide<br />
CATALOG NO.: 2241P<br />
SEQUENCE: NH 2 -DDPVRTAQVPSPPRGK-OH<br />
LOCATION:<br />
Amino acids 994 to 1009 of human AcinusL, 267 to 282 of human AcinusS’, or 236 to 251 of human<br />
AcinusS, which are identical to those of mouse Acinus (1). The selected antigenic sequence is located near<br />
the N-terminus of active peptide p17.<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of Acinus (catalog number 2241). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated<br />
protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
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Caspase-13 peptide<br />
CATALOG NO.: 2245P<br />
SEQUENCE: NH 2 -ETVAGPDESVGSAAT-OH<br />
LOCATION: Amino acids 91 to 105 of human Acaspase-13 (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of caspase-13 (catalog number 2245). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Humke EW, Ni J, Dixit VM. ERICE, a novel FLICE-activatable caspase. J Biol Chem<br />
1998;273:15702-7
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techsupport@prosci-inc.com<br />
BACE2 (NT) peptide<br />
CATALOG NO.: 2247P<br />
SEQUENCE: NH 2 -APTPGPGTPAERHADG-OH<br />
LOCATION: Amino acids 44 to 59 of human BACE2 (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of BACE2 (catalog number 2247). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity.<br />
Nature 1999;402:533-7
13100 Kirkham Way<br />
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techsupport@prosci-inc.com<br />
BACE2 (CT) peptide<br />
CATALOG NO.: 2249P<br />
SEQUENCE: NH 2 -CQRRPRDPEVVNDESS-OH<br />
LOCATION: Amino acids 496 to 511 of human BACE2 (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of BACE2 (catalog number 2249). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity.<br />
Nature 1999;402:533-7
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
BACE (CT) peptide<br />
CATALOG NO.: 2253P<br />
SEQUENCE: NH 2 -CLRQQHDDFADDISLLK-OH<br />
LOCATION: Amino acids 485 to 501 of human BACE (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of BACE (catalog number 2253). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase cleavage of Alzheimer's amyloid<br />
precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
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AIF (IN) peptide<br />
CATALOG NO.: 2267P<br />
SEQUENCE: NH 2 -PKSATEQSGTGIRSE-OH<br />
LOCATION:<br />
Amino acids 517 to 531 of human AIF, which are identical to corresponding amino acids of mouse and<br />
rat AIF (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of AIF (catalog number 2267). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosisinducing<br />
factor. Nature 1999;397:441-6
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
FLASH (CT) peptide<br />
CATALOG NO.: 2271P<br />
SEQUENCE: NH 2 -SERFQQLMKLFEKSKC-OH<br />
LOCATION: Amino 1253 to 1268 of human FLASH (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of FLASH (catalog number 2271). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains.<br />
Nature 1999;401:662-3
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
F1Aα peptide<br />
CATALOG NO.: 2279P<br />
SEQUENCE: NH 2 -DINYQDQIPRTLEE-OH<br />
LOCATION: Amino acids 609 to 622 of human F1Aα, ?which are identical to the corresponding<br />
sequences of mouse FEM1β (1,2).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of F1Aα (catalog number 2279). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu VC. F1Aalpha, a death receptor-binding<br />
protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate<br />
that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8.<br />
2. Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene.<br />
Biochem Biophys Res Commun 2000;267(1):317-20
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
RIP3 peptide<br />
CATALOG NO.: 2283P<br />
SEQUENCE: NH 2 -AQFGRGRGWQPFHK-OH<br />
LOCATION: Amino acids 473 to 486 of murine RIP3 (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of RIP3 (catalog number 2283). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not<br />
contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-? B. Mol Cell Biol.<br />
1999;19(10):6500-8.
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
ASC peptide<br />
CATALOG NO.: 2287P<br />
SEQUENCE: NH 2 -RESQSYLVEDLERS-OH<br />
LOCATION: Amino acids 182 to 195 of human ASC (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of ASC (catalog number 2287). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T,<br />
Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic<br />
leukemia HL-60 cells. J Biol Chem. 1999;274(48):33835-8.
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Bnip3L (IN) peptide<br />
CATALOG NO.: 2289P<br />
SEQUENCE: NH 2 -DAQHESGQSSSRGSSH-OH<br />
LOCATION: Amino acids 77 to 92 of human origin, which are identical to those of mouse Bnip3L (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of Bnip3L (catalog number 2289). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial<br />
proteins. J Biol Chem 1999;274(1):7-10
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DcR1 (ED2) peptide<br />
CATALOG NO.: 2299P<br />
SEQUENCE: NH 2 -CKEGTFRNENSPE-OH<br />
LOCATION: Amino acids 111 to 123 at extracellular domain of human DcR1 precursor (1,2).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of DcR1 (catalog number 2299). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for<br />
TRAIL. Science 1997;277:815-8<br />
3. Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of<br />
signaling and decoy receptors. Science 1997;277:818-21
13100 Kirkham Way<br />
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www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
AIF (CT) peptide<br />
CATALOG NO.: 2301P<br />
SEQUENCE: NH 2 -KDGEQHEDLNEVAK-OH<br />
LOCATION: Amino acids 593 to 606 of human AIF (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of AIF (catalog number 2301). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosisinducing<br />
factor. Nature 1999;397:441-6
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DAP Kinase 2 peptide<br />
CATALOG NO.: 2323P<br />
SEQUENCE: NH 2 -ARRKALHPRRRSSTS-OH<br />
LOCATION: Amino acids 356 to 370 of human DAPK2 (1,2).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of DAP kinase 2 (catalog number 2323). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with equal<br />
volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated<br />
protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its<br />
catalytic activity. Oncogene 1999;18(23):3471-80<br />
2. Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a<br />
novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Caspase-12 (NT) peptide<br />
CATALOG NO.: 2325P<br />
SEQUENCE: NH 2 -AARRTHERDPIYKIKG-OH<br />
LOCATION: Amino acids 2 to 17 of murine caspase-12 (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of caspase-12 (catalog number 2325). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmicreticulum-specific<br />
apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-103.
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Caspase-12 (IN) peptide<br />
CATALOG NO.: 2327P<br />
SEQUENCE: NH 2 -CTPSSPSESKRKVEDDE-OH<br />
LOCATION: Amino acids 100 to 116 of murine caspase-12 (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of caspase-12 (catalog number 2327). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmicreticulum-specific<br />
apoptosis and cytotoxicity by amyloid-beta. Nature 2000;403:98-103.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IKKε/IKK-i (CT) peptide<br />
CATALOG NO.: 2329P<br />
SEQUENCE: NH 2 -NRIIERLNRVPAPPDV-OH<br />
LOCATION: Amino acids 701 to 716 of human IKKε/IKK-i (1,2).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IKKε/IKK-i (catalog number 2329). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Peters RT, Liao SM, Maniatis T. IKKepsilon is part of a novel PMA-inducible IkappaB kinase complex.<br />
Mol Cell 2000;5(3):513-22<br />
2. Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi Y, Kanamaru A, Akira S. IKK-i, a<br />
novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases. Int Immunol 1999;11(8):1357-<br />
62
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techsupport@prosci-inc.com<br />
IKKγ/NEMO (NT) peptide<br />
CATALOG NO.: 2335P<br />
SEQUENCE: NH 2 -CQYQAPDMDTLQIHVME-OH<br />
LOCATION: Amino acids 400 to 416 of human IKKγ (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IKKγ (catalog number 2335). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the<br />
IkappaB kinase complex. Nature 1998;395(6699):297-300<br />
2. Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A.<br />
Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB<br />
activation. Cell. 1998;93(7):1231-40.
13100 Kirkham Way<br />
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DC-SIGN (CT) peptide<br />
CATALOG NO.: 2347P<br />
SEQUENCE: NH 2 -CSRDEEQFLSPAPATPNPPPA-OH<br />
LOCATION: Amino acids 384 to 404 of human DC-DIGN (1).<br />
Application:<br />
The peptide is used for blocking the antibody activity of DC-SIGN (catalog number 2347). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with an<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-<br />
binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
DC-SIGN (ED) peptide<br />
CATALOG NO.: 2349P<br />
SEQUENCE: NH 2 -CYFMSNSQRNWHDSITA-OH<br />
LOCATION: Amino acids 277 to 293 of human DC-DIGN (1).<br />
Application:<br />
The peptide is used for blocking the antibody activity of DC-SIGN (catalog number 2347). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-<br />
binding protein that enhances trans-infection of T cells. Cell. 2000;100:587-97.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
NAK/TBK1 (CT) peptide<br />
CATALOG NO.: 2351P<br />
SEQUENCE: NH 2 -ERFGSLTMDGGLRNVD-OH<br />
LOCATION: Amino acids 712 to 727 of human NAK/TBK1 (1,2)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of NAK/TBK1 (catalog number 2351). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with an equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Pomerantz JL, Baltimore D. NF-kappaB activation by a signaling complex containing TRAF2, TANK<br />
and TBK1, a novel IKK-related kinase. EMBO J 1999;18(23):6694-704<br />
2. Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S, Nakayama K, Kaneko Y, Nimura Y,<br />
Motoyama N, Ikeda K, Karin M, Nakanishi M. NAK is an IkappaB kinase-activating kinase. Nature<br />
2000;404(6779):778-82
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
IRAK-M peptide<br />
CATALOG NO.: 2355P<br />
SEQUENCE: NH 2 -CSSKFSWDEYEQYKKE-OH<br />
LOCATION: Amino acids 581 to 596 of human IRAK-M (1)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IRAK-M (catalog number 2255). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Wesche H, Gao X, Li X, Kirschning CJ, Stark GR, Cao Z. IRAK-M is a novel member of the<br />
Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J Biol Chem. 1999;274(27):19403-10.
13100 Kirkham Way<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
p53R2 (NT) peptide<br />
CATALOG NO.: 2383P<br />
SEQUENCE: NH 2 -GDPERPEAAGLDQDER-OH<br />
LOCATION: Amino acids 2 to 17 of human p53R2 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of p53R2 (catalog number 2383). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.<br />
Science. 1998;282:1893-7.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Chk2 (NT) peptide<br />
CATALOG NO.: 2391P<br />
SEQUENCE: NH 2 -SRESDVEAQQSHGSSAC-OH<br />
LOCATION: Amino acids 2 to 18 of human Chk2 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of Chk2 (catalog number 2391). It usually blocks<br />
the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody<br />
for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.<br />
Science. 1998;282:1893-7.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Smac/DIABLO (CT) peptide<br />
CATALOG NO.: 2409P<br />
SEQUENCE: NH 2 -EERAESEQEAYLRED-OH<br />
LOCATION: Amino acids 225 to 239 of human Smac/DIABLO (1)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of mSmac/DIABLO (catalog number 2409). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with equal<br />
volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein that promotes cytochrome c-<br />
dependent caspase activation by eliminating IAP inhibition. Cell. 2000;102(1):33-42.
13100 Kirkham Way<br />
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Tel: 858-513-2638<br />
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techsupport@prosci-inc.com<br />
mSmac/DIABLO (CT) peptide<br />
CATALOG NO.: 2411P<br />
SEQUENCE: NH 2 - SDEGADQEEEAYLRED-OH<br />
LOCATION: Amino acids 222 to 237 of murine Smac/DIABLO (1)<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of mSmac/DIABLO (catalog number 2411). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with equal<br />
volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux<br />
DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and<br />
antagonizing IAP proteins. Cell. 2000;102(1):43-53.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CATALOG NO.: 2415P<br />
APRIL (ED2) (TALL-2/TRDL-1) peptide<br />
SEQUENCE: NH 2 -CIRSMPSHPDRAYNS-OH<br />
LOCATION: Amino acids 196 to 210 of human APRIL (1-3).<br />
Application:<br />
The peptide is used for blocking the antibody activity of APRIL (catalog number 2415). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor<br />
family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.<br />
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is downregulated<br />
by mitogens. J Leukoc Biol. 1999;65(5):680-3.<br />
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand,<br />
stimulates cell death. Cancer Res. 2000;60(4):1021-7.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
PID/MTA2 peptide<br />
CATALOG NO.: 2443P<br />
SEQUENCE: PAPSHPASTNEPIVLED<br />
LOCATION: Amino acids 652 to 668 of human PID/MTA2 (1)<br />
APPLICATION:<br />
The peptide is used for blocking the activity of PID/MTA2 (catalog number 2443). Incubating the<br />
peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity<br />
in Western blotting.<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Luo J, Su F, Chen D, Shiloh A, Gu W. Deacetylation of p53 modulates its effect on cell growth and<br />
apoptosis. Nature. 2000;408:377-81.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
MTBP (IN2) peptide<br />
CATALOG NO.: 2447P<br />
SEQUENCE: NH 2 -GAVECFEEEDSNSRESLS-OH<br />
LOCATION: Amino acids 122 to 139 of human MTBP (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of MTBP (catalog number 2247). It usually<br />
blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of<br />
antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130 mM<br />
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.<br />
REFERENCE:<br />
1. Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein (MTBP) binds to MDM2 and induces a<br />
G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
PERP peptide<br />
CATALOG NO.: 2451P<br />
SEQUENCE: NH 2 -EDDLLGNAKPRYFYTSA-OH<br />
LOCATION: Amino acids 175 to 193 of human PERP (1,2).<br />
Application:<br />
The peptide is used for blocking the antibody activity of PERP (catalog number 2451). It can<br />
block the antibody activity completely in Western blot by incubating the peptide with equal volume<br />
of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Boyd MT, Vlatkovic N, Haines DS. A novel cellular protein (MTBP) binds to MDM2 and<br />
induces a G1 arrest that is suppressed by MDM2. J Biol Chem. 2000;275(41):31883-90.<br />
2. Attardi,L.D., Reczek,E.E., Cosmas,C., et al. PERP, an apoptosis-associated target of p53,<br />
is a novel member of the PMP-22/gas3 family. Genes Dev. 2000;14 (6):704-718
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-21 (IN) peptide<br />
CATALOG NO.: 2465P<br />
SEQUENCE: NH 2 -TCPSCDSYEKKPPKE-OH<br />
LOCATION: Amino acids 121 to 135 of human IL-21 precursor (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IL-21 (catalog number 2465). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Arrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK<br />
cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
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Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-21R (ED) peptide<br />
CATALOG NO.: 2469P<br />
SEQUENCE: NH 2 -NITDQSGNYSQECGS-OH<br />
LOCATION: Amino acids 97 to 111 of human IL-21R precursor (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IL-21R (catalog number 2469). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Arrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK<br />
cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-21R (NT) peptide<br />
CATALOG NO.: 2471P<br />
SEQUENCE: NH 2 -CPDLVCYTDYLQT-OH<br />
LOCATION: Amino acids 20 to 32 of human IL-21R precursor (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IL-21R (catalog number 2471). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Arrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK<br />
cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-22R (CT) peptide<br />
CATALOG NO.: 2489P<br />
SEQUENCE: NH 2 -DSLFRGLALTVQWES-OH<br />
LOCATION: Amino acids 560 to 574 of human IL-22R precursor (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IL-22R (catalog number 2489). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cellderived<br />
inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J<br />
Immunol. 2000;164(4):1814-9.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
IL-22R (NT) peptide<br />
CATALOG NO.: 2497P<br />
SEQUENCE: NH 2 -QSSNFENILTWDSGPE-OH<br />
LOCATION: Amino acids 31 to 46 of human IL-22R precursor (1).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of IL-22R (catalog number 2497). It<br />
usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cellderived<br />
inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J<br />
Immunol. 2000;164(4):1814-9.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CIKS/Act1 (CT) peptide<br />
CATALOG NO.: 2499P<br />
SEQUENCE: NH 2 -REEEYVAPPRGPLPT-OH<br />
LOCATION: Amino acids 554 to 568 of human CIKS (1,2).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of CIKS/Act1 (catalog number 2499).<br />
It usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to<br />
Ikappa B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.<br />
2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR.<br />
Act1, an NF-kappa B activating protein. Proc Natl Acad Sci USA. 2000;97(19):10489-93.
13100 Kirkham Way<br />
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Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
CIKS/Act1 (NT) peptide<br />
CATALOG NO.: 2501P<br />
SEQUENCE: NH 2 -PPQLQETRMNRSIP-OH<br />
LOCATION: Amino acids 2 to 15 of human CIKS (1,2).<br />
APPLICATION:<br />
The peptide is used for blocking the antibody activity of CIKS/Act1 (catalog number 2501).<br />
It usually blocks the antibody activity completely in Western blot by incubating the peptide with<br />
equal volume of antibody for 30 min at 37°C<br />
For research use only.<br />
STORAGE:<br />
It is supplied as 50 µg at 200 µg/ml, in PBS pH7.2 (10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 130<br />
mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C,<br />
stable for one year.<br />
REFERENCE:<br />
1. Leonardi A, Chariot A, Claudio E, Cunningham K, Siebenlist U. CIKS, a connection to<br />
Ikappa B kinase and stress-activated protein kinase. Proc Natl Acad Sci USA. 2000;97(19):10494-9.<br />
2. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, Stark GR.<br />
Act1, an NF-kappa B activating protein. Proc Natl Acad Sci USA. 2000;97(19):10489-93.
lysate Data Sheets
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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HeLa Cell Lysate<br />
<strong>Catalog</strong> No: 1201<br />
Cell line:<br />
HeLa-S3 (Human cervix epithelioid carcinoma)<br />
Growth media: Jokliks-MEM and 5% NCS (Newborn calf serum).<br />
Cell lysate preparation:<br />
HeLa cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
HeLa cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
A431 Cell Lysate<br />
<strong>Catalog</strong> No: 1202<br />
Cell line:<br />
A431 (Human epidermoid carcinoma)<br />
Growth media: DMEM and 10% FBS.<br />
Cell lysate preparation:<br />
A431 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
A431 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
A549 Cell Lysate<br />
<strong>Catalog</strong> No: 1203<br />
Cell line:<br />
A549 (Human lung carcinoma)<br />
Growth media: DMEM and 10% NCS (Newborn calf serum)<br />
Cell lysate preparation:<br />
A549 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
A549 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
K562 Cell Lysate<br />
<strong>Catalog</strong> No: 1204<br />
Cell line:<br />
K562 (Human chronic myelogenous leukemia)<br />
Growth media: RPMI & 10% NCS (Newborn calf serum)<br />
Cell lysate preparation:<br />
K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
K562 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Jurkat Cell Lysate<br />
<strong>Catalog</strong> No: 1205<br />
Cell line:<br />
Jurkat, clone E6-1 (Human acute T cell leukemia)<br />
Growth media: RPMI & 10% NCS (Newborn calf serum)<br />
Cell lysate preparation:<br />
Jurkat cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Jurkat cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
MOLT4 Cell Lysate<br />
<strong>Catalog</strong> No: 1206<br />
Cell line:<br />
MOLT4 (Human acute lymphoblastic leukemia)<br />
Growth media: RPMI and 10% FBS<br />
Cell lysate preparation:<br />
MOLT4 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
MOLT4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Raji Cell Lysate<br />
<strong>Catalog</strong> No: 1207<br />
Cell line:<br />
Raji (Human Burkitt's lymphoma)<br />
Growth media: RPMI & 10% NCS (Newborn calf serum)<br />
Cell lysate preparation:<br />
Raji cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Raji cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
THP-1 Cell Lysate<br />
<strong>Catalog</strong> No: 1208<br />
Cell line:<br />
THP-1 (Human monocyte)<br />
Growth media: RPMI & 10% FBS<br />
Cell lysate preparation:<br />
THP-1 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
THP-1 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
HL60 Cell Lysate<br />
<strong>Catalog</strong> No: 1209<br />
Cell line:<br />
HL60 (Human promyelocytic leukemia)<br />
Growth media: RPMI & 10% FBS (Fetal bovine serum)<br />
Cell lysate preparation:<br />
HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing 5%<br />
β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
293 Cell Lysate<br />
<strong>Catalog</strong> No: 1210<br />
Cell line:<br />
293 (Human transformed primary embryonal kidney)<br />
Growth media: RPMI & 10% FBS (Fetal bovine serum)<br />
Cell lysate preparation:<br />
293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
293 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
HepG2 Cell Lysate<br />
<strong>Catalog</strong> No: 1211<br />
Cell line:<br />
HepG2 (Human hepatocellular carcinoma)<br />
Growth media: DMEM & 10% FBS (Fetal bovine serum)<br />
Cell lysate preparation:<br />
HepG2 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium<br />
chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
HepG2 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
3T3 Cell Lysate<br />
<strong>Catalog</strong> No: 1212<br />
Cell line:<br />
BALB/3T3 clone A31 (Mouse embryo)<br />
Growth media: DMEM & 10% FBS (Fetal bovine serum)<br />
Cell lysate preparation:<br />
BALB/3T3 clone A31 cell lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
BALB/3T3 clone A31 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
T24 Cell Lysate<br />
<strong>Catalog</strong> No: 1213<br />
Cell line:<br />
T24 (Transitional-cell human bladder carcinoma)<br />
Growth media: DMEM & 10% FBS (Fetal bovine serum)<br />
Cell lysate preparation:<br />
T24 (Transitional-cell human bladder carcinoma) cell lysate was prepared by homogenization<br />
in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM<br />
ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1%<br />
sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin).<br />
Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad<br />
protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH<br />
6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-<br />
mercaptoethanol.<br />
Application:<br />
T24 (Transitional-cell human bladder carcinoma) cell lysate is ready to load on SDS-PAGE<br />
for Western blotting, 20 µg per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
SW1353 Cell Lysate<br />
<strong>Catalog</strong> No: 1214<br />
Cell line:<br />
SW1353 (Human humerus chondrosarcoma)<br />
Growth media: DMEM & 10% FBS (Fetal bovine serum)<br />
Cell lysate preparation:<br />
SW1353 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
SW1353 cell lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
U937 Whole Cell Lysate<br />
<strong>Catalog</strong> No: 1215<br />
Cell line:<br />
U937 (Human histiocytic lymphoma)<br />
Growth media: DMEM & 10% NCS (Newborn Calf serum)<br />
Cell lysate preparation:<br />
U937 (Human histiocytic lymphoma) cell lysate was prepared by homogenization in modified<br />
RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic<br />
acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1%<br />
sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate<br />
was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
U937 (Human histiocytic lymphoma) cell lysate is ready to load on SDS -PAGE for Western<br />
blotting, 20 µg per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate, 200 µg per vial at 100 µg at 2 mg/ml, in 1 x SDS sample<br />
buffer containing 5% β-mercaptoethanol. Storage at -20°C will be stable for three months, storage<br />
at –70°C will be stable for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
PC-3 Whole Cell Lysate<br />
<strong>Catalog</strong> No: 1216<br />
Cell line:<br />
PC-3 (Human prostate adenocarcinoma)<br />
Growth media: DMEM/Hams F12 & 10% NCS (Newborn Calf serum)<br />
Cell lysate preparation:<br />
PC-3 (Human prostate adenocarcinoma) cell lysate was prepared by homogenization in<br />
modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM<br />
ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1%<br />
sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin).<br />
Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad<br />
protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH<br />
6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-<br />
mercaptoethanol.<br />
Application:<br />
PC-3 (Human prostate adenocarcinoma) cell lysate is ready to load on SDS-PAGE for<br />
Western blotting, 20 µg per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate, 200 µg per vial at 100 µg at 2 mg/ml, in 1 x SDS sample<br />
buffer containing 5% β-mercaptoethanol. Storage at -20°C will be stable for three months, storage<br />
at –70°C will be stable for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
MDA-MB-361 Whole Cell Lysate<br />
<strong>Catalog</strong> No: 1217<br />
Cell line:<br />
MDA-MB-361 (Human breast adenocarcinoma)<br />
Growth media: DMEM/Hams F12 & 10% NCS (Newborn Calf serum)<br />
Cell lysate preparation:<br />
MDA-MB-361 (Human breast adenocarcinoma) cell lysate was prepared by homogenization<br />
in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM<br />
ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1%<br />
sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin).<br />
Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad<br />
protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH<br />
6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-<br />
mercaptoethanol.<br />
Application:<br />
MDA-MB-361 (Human breast adenocarcinoma) cell lysate is ready to load on SDS-PAGE for<br />
Western blotting, 20 µg per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate, 200 µg per vial at 100 µg at 2 mg/ml, in 1 x SDS sample<br />
buffer containing 5% β-mercaptoethanol. Storage at -20°C will be stable for three months, storage<br />
at –70°C will be stable for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Heart Tissue Lysate<br />
<strong>Catalog</strong> No: 1301<br />
Source: 38 year old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human heart tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Lung Tissue Lysate<br />
<strong>Catalog</strong> No: 1302<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human lung tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Brain Tissue Lysate<br />
<strong>Catalog</strong> No: 1303<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human brain tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Liver Tissue Lysate<br />
<strong>Catalog</strong> No: 1304<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human liver tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Kidney Tissue Lysate<br />
<strong>Catalog</strong> No: 1305<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150<br />
mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human kidney tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per<br />
lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Spleen Tissue Lysate<br />
<strong>Catalog</strong> No: 1306<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150<br />
mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human spleen tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per<br />
lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Pancreas Tissue Lysate<br />
<strong>Catalog</strong> No: 1307<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150<br />
mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human pancreas tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per<br />
lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1308<br />
Human Small Intestine Tissue Lysate<br />
Source: 38-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human small intestine tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human small intestine tissue lysate is ready to load on SDS -PAGE for Western blotting, 20<br />
µg per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1321<br />
Human Lung Tumor Tissue Lysate<br />
Source: 28-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human lung tumor (adenocarcinoma) tissue lysate was prepared by homogenization in lysis<br />
buffer (10 mM HEPES pH7.9, 1.5 mM MgCl 2 , 10 mM KCl, 1 mM ethylenediaminetetraacetic acid,<br />
10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was<br />
removed by centrifugation. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50<br />
mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue)<br />
containing 5% β-mercaptoethanol.<br />
Application:<br />
Human lung tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Human Brain Tumor Tissue Lysate<br />
<strong>Catalog</strong> No: 1322<br />
Source: 75-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human brain tumor (Meningeal tumor) tissue lysate was prepared by homogenization in lysis<br />
buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml aprotinin, 5<br />
µg/ml leupeptin, 1% Triton x-100, 1% DOC, 0.1% SDS). Tissue and cell debris was removed by<br />
centrifugation. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl<br />
pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-<br />
mercaptoethanol.<br />
Application:<br />
Human brain tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1323<br />
Human Breast Tumor Tissue Lysate<br />
Source: 56-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human breast tumor (moderately differentiated invasive ductal carcinoma) tissue lysate was<br />
prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl 2 , 10 mM KCl, 1<br />
mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease<br />
inhibitors). Tissue and cell debris was removed by centrifugation. The tissue lysate was boiled for<br />
5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium<br />
dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human breast tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1324<br />
Human Kidney Tumor Tissue Lysate<br />
Source: 44-year-old female<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human kidney tumor (renal cell carcinoma) tissue lysate was prepared by homogenization in<br />
lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl 2 , 10 mM KCl, 1 mM ethylenediaminetetraacetic<br />
acid, 10% glycerol, 1% NP -40, and a cocktail of protease inhibitors). Tissue and cell debris was<br />
removed by centrifugation. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50<br />
mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue)<br />
containing 5% β-mercaptoethanol.<br />
Application:<br />
Human kidney tumor tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1341-N<br />
Human Colon Normal Tissue Lysate<br />
Source: Human adult colon<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human colon normal tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human colon normal tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer<br />
containing 5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1341-T<br />
Human Colon Tumor Tissue Lysate<br />
Source: Human adult colon<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human colon tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1342-N<br />
Human Liver Normal Tissue Lysate<br />
Source: Human adult liver<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human liver normal tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human liver normal tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1342-T<br />
Human Liver Tumor Tissue Lysate<br />
Source: Human adult liver<br />
This material has been tested by accepted techniques and has been found to be negative<br />
for HBsAg, HIV 1/2, and HCV.<br />
Tissue lysate preparation:<br />
Human liver tumor tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Human liver tumor tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Mouse Heart Tissue Lysate<br />
<strong>Catalog</strong> No: 1401<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse heart tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Mouse Lung Tissue Lysate<br />
<strong>Catalog</strong> No: 1402<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse lung tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Mouse Brain Tissue Lysate<br />
<strong>Catalog</strong> No: 1403<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse brain tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Mouse Liver Tissue Lysate<br />
<strong>Catalog</strong> No: 1404<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse liver tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane<br />
is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Mouse Kidney Tissue Lysate<br />
<strong>Catalog</strong> No: 1405<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150<br />
mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse kidney tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per<br />
lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Mouse Spleen Tissue Lysate<br />
<strong>Catalog</strong> No: 1406<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150<br />
mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per<br />
lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1407<br />
Mouse Skeletal Muscle Tissue Lysate<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse skeletal muscle tissue lysate was prepared by homogenization in modified RIPA<br />
buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid,<br />
1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse skeletal muscle lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per<br />
lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1408<br />
Mouse Small Intestine Tissue Lysate<br />
Source:<br />
White laboratory mice<br />
Tissue lysate preparation:<br />
Mouse small intestine tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Mouse small intestine tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Rat Heart Tissue Lysate<br />
<strong>Catalog</strong> No: 1461<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat heart tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat heart tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Rat Lung Tissue Lysate<br />
<strong>Catalog</strong> No: 1462<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat lung tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Rat Brain Tissue Lysate<br />
<strong>Catalog</strong> No: 1463<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat brain tissue lysate is ready to load on SDS -PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Rat Liver Tissue Lysate<br />
<strong>Catalog</strong> No: 1464<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat liver tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Rat Kidney Tissue Lysate<br />
<strong>Catalog</strong> No: 1465<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat kidney tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
Rat Spleen Tissue Lysate<br />
<strong>Catalog</strong> No: 1466<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM<br />
sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is<br />
recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
Fax: 858-513-2692<br />
techsupport@prosci-inc.com<br />
<strong>Catalog</strong> No: 1467<br />
Rat Skeletal Muscle Tissue Lysate<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat skeletal muscle tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat skeletal muscle tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
13100 Kirkham Way<br />
Suite 207<br />
Poway, CA 92064<br />
www.prosci-inc.com<br />
Toll Free: 888-513-9525<br />
Tel: 858-513-2638<br />
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Rat Small Intestine Tissue Lysate<br />
<strong>Catalog</strong> No: 1468<br />
Source:<br />
White laboratory rats<br />
Tissue lysate preparation:<br />
Rat small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer<br />
(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM<br />
phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium<br />
dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by<br />
centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue<br />
lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1%<br />
sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.<br />
Application:<br />
Rat small Intestine tissue lysate is ready to load on SDS-PAGE for Western blotting, 20 µg<br />
per lane is recommended for mini gel.<br />
Storage:<br />
It is supplied as whole cell lysate at 100 µg at 2 mg/ml in 1 x SDS sample buffer containing<br />
5% β-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.<br />
For research use only.
Order forms
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Suite 207<br />
Poway, CA 92064<br />
orders@prosci-inc.com<br />
Tel: 858.513.2638<br />
Fax: 858.513.2692<br />
Toll Free: 888.513.9525<br />
www.prosci-inc.com<br />
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PRODUCTS<br />
<strong>Catalog</strong> No. Description Size Quantity
Protocols
WESTERN BLOT PROCEDURE<br />
1) Load 20 to 25 microgram of whole cell lysate per lane in an SDS-PAGE mini gel.<br />
2) Run at 20 mA per gel untill the dye front is close to the bottom.<br />
3) Transfer the proteins to a nitrocellulose membrane (S&S NCTM) at 250 mA in<br />
transfer buffer for 1-4 h, depending on the size of the target protein.<br />
4) Incubate the blot with blocking buffer (5% non-fat dry milk in TBS) overnight at 4 o C<br />
or 2 h at room temperature (RT).<br />
5) Incubate the blot with primary antibody (diluted 1:250 to 1:1000 in blocking buffer)<br />
for 1 h in blocking buffer at RT.<br />
6) Wash the blot 3 x 10 min in washing buffer (TBS containing 0.1% Tween 20) with<br />
shaking.<br />
7) Incubate blot with anti-rabbit IgG-HRP conjugate (Sigma) (diluted 1:10,000 -<br />
1:2,000 in blocking buffer) for 1 h in blocking buffer at RT.<br />
8) Wash 3 x 10 min in washing buffer with shaking.<br />
9) Drain washing buffer, add ECL solution (Amersham) and develop for 1 min.<br />
10) Expose to X-ray film for 1 to 30 min.<br />
TBS: • 125 mM NaCl<br />
• 25 mM Tris pH 8.0<br />
SDS/Running Buffer: • 25 mM Tris<br />
• 192 mM Glycine<br />
• 1% SDS<br />
Transfer Buffer: • 20 mM Tris pH 8.0<br />
• 150 mM Glycine<br />
• 20% methanol
CELL LYSATE PROCEDURE<br />
1) Wash the cell pellets with ice-cold PBS.<br />
2) Add 1 ml of RIPA buffer per 10 8 cells and incubate on ice for 20 min, vortex 2-3<br />
times, briefly.<br />
3) Aliquat cell lysate into 1.5 ml microfuge tubes and centrifuge at 13,000 rpm, 4 o L for<br />
5 min in an Eppendorf microfuge.<br />
4) Transfer the supernatant into clean tubes. Measure the protein concentration with<br />
BioRad protein assay.<br />
5) Adjust the concentration to 5 mg/ml with RIPA lysis buffer.<br />
6) Add an equal volume of 2 x SDS sample buffer into the cell lysate. Boil for 5 min.<br />
7) Store at -20 o C for daily use or -80 o C for long term storage.<br />
RIPA Buffer: • 50 mM Tris-HCl pH 7.4<br />
• 150 mM NaCl<br />
• 1 mM PMSF<br />
• 1 mM EDTA<br />
• 5 µg/ml Aprotinin<br />
• 5 µg/ml Leupeptin<br />
• 1% Triton -100<br />
• 1% Sodium deoxycholate<br />
• 0.1% SDS<br />
2 x SDS/Sample buffer: • 100 mM Tris<br />
• 25% glycerol<br />
• 2% SDS<br />
• 0.01% bromphenol blue<br />
• Adjust to pH 6.8<br />
• Add 2-ME to 10%