HT Protein Charge Variant Kit User Guide - PerkinElmer
HT Protein Charge Variant Kit User Guide - PerkinElmer
HT Protein Charge Variant Kit User Guide - PerkinElmer
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<strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> <strong>Kit</strong> <strong>User</strong> <strong>Guide</strong><br />
Running the Assay<br />
1. Click the Run button in the upper left corner of the LabChip GX software.<br />
2. The Start Run dialog box will appear. At the top of the box are tabs labeled Run, Output, and Advanced.<br />
3. In the Run tab, select the appropriate Assay, Plate Name, and Well Pattern. For selection of the<br />
appropriate Assay (<strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> 68s, <strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> 90s, or <strong>HT</strong> <strong>Protein</strong><br />
<strong>Charge</strong> <strong>Variant</strong> 110s), refer to Table 2.<br />
4. In the Output tab, select the destination of the file and the filename convention. We recommend including<br />
Running Buffer pH in the File prefix.<br />
5. In the Advanced tab, select the number of times each well is to be sampled and the sample names.<br />
6. Click Start to begin the run.<br />
Table 2: Recommended Run Conditions<br />
pI of Main <strong>Variant</strong> Running Buffer pH Assay<br />
9.5 - 9.1 7.2 <strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> 68s<br />
9.0 - 8.7 6.2 <strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> 68s<br />
8.6 - 8.0 5.9 <strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> 90s<br />
7.9 - 7.0 5.6 <strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> 110s<br />
The pH values listed in Table 2 are recommendations for achieving high resolution of charge variants<br />
within the time allowed by the indicated assay. If required, the resolution may be increased by increasing<br />
the pH. However, migration speeds decrease with increasing pH, and thus a longer assay may be<br />
required.<br />
_________________________________________________________________________________________<br />
Caliper - a <strong>PerkinElmer</strong> Company Page 7 of 18 PN: CLS135474 Rev. 02<br />
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