HT Protein Charge Variant Kit User Guide - PerkinElmer
HT Protein Charge Variant Kit User Guide - PerkinElmer
HT Protein Charge Variant Kit User Guide - PerkinElmer
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<strong>HT</strong> <strong>Protein</strong> <strong>Charge</strong> <strong>Variant</strong> <strong>Kit</strong> <strong>User</strong> <strong>Guide</strong><br />
Specifications<br />
Sample Type<br />
Monoclonal antibody (mAb)<br />
pI Range 7.0 - 9.5<br />
Amount of Sample Required<br />
Resolution<br />
Reproducibility<br />
Carryover<br />
Assay Run Time<br />
Number of Samples per <strong>Kit</strong><br />
25 µL with concentration between 0.5 - 10 mg/mL<br />
(12.5 µg to 250 µg of mAb, total)<br />
Optimal concentration: 2 mg/mL<br />
Comparable to IEX and conventional CZE<br />
CV < 5% for varying concentration from 1 - 3 mg/mL<br />
CV < 3% at constant concentration<br />
< 0.5% of previous sample can be detected in following<br />
sample.<br />
1.8 - 3 hr for a 96-well plate.<br />
Three Assay durations: 68 s, 90 s, and 110 s.<br />
120 samples<br />
Number of Samples per Chip Prep<br />
Chip Life Time 1<br />
96 samples<br />
500 samples per chip<br />
1 Expected chip lifetime is based on use under normal laboratory conditions and adherence to Caliper preparation protocols, sample guidelines and storage<br />
conditions. Individual results may vary.<br />
_________________________________________________________________________________________<br />
Caliper - a <strong>PerkinElmer</strong> Company Page 2 of 18 PN: CLS135474 Rev. 02<br />
68 Elm Street<br />
Hopkinton, MA 01748-1668<br />
1-877-LABCHIP (1-877-522-2447)