HT Protein Charge Variant Kit User Guide - PerkinElmer

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HT Protein Charge Variant Kit User Guide Specifications Sample Type Monoclonal antibody (mAb) pI Range 7.0 - 9.5 Amount of Sample Required Resolution Reproducibility Carryover Assay Run Time Number of Samples per Kit 25 µL with concentration between 0.5 - 10 mg/mL (12.5 µg to 250 µg of mAb, total) Optimal concentration: 2 mg/mL Comparable to IEX and conventional CZE CV < 5% for varying concentration from 1 - 3 mg/mL CV < 3% at constant concentration < 0.5% of previous sample can be detected in following sample. 1.8 - 3 hr for a 96-well plate. Three Assay durations: 68 s, 90 s, and 110 s. 120 samples Number of Samples per Chip Prep Chip Life Time 1 96 samples 500 samples per chip 1 Expected chip lifetime is based on use under normal laboratory conditions and adherence to Caliper preparation protocols, sample guidelines and storage conditions. Individual results may vary. _________________________________________________________________________________________ Caliper - a PerkinElmer Company Page 2 of 18 PN: CLS135474 Rev. 02 68 Elm Street Hopkinton, MA 01748-1668 1-877-LABCHIP (1-877-522-2447)
HT Protein Charge Variant Kit User Guide Kit Contents HT Protein Charge Variant Kit (P/N: CLS760670) Items at -20 ºC (HT Protein Charge Variant Labeling Kit) Vial Quantity Labeling Buffer Orange 1 vial, 1 mL Dye Concentrate Items at 4 ºC (HT Protein Charge Variant Buffer Kit) Blue Bottle 5 vials, 5 µL (24 samples / vial) Quantity Running Buffer pH 5.6 Red 1 bottle, 25 mL Running Buffer pH 7.2 Green 1 bottle, 25 mL Storage Buffer Purple 1 bottle, 25 mL Consumable Items Supplier Number Quantity Ladder Tubes Genemate, Cat. # C-3258-1 10, 0.2 mL PCR tubes Centrifuge Tubes Eppendorf, Cat. # 022363352 5, 2.0 mL centrifuge tubes Detection Window Cleaning Cloth VWR, Cat. # 21912-046 1 clean room cloth Swab ITW Texwipe®, Cat. # TX758B 3 Buffer Tubes E&K Scientific, Cat. # 697075-NC 10, 0.75 mL tubes HT DNA5K/RNA/CZE LabChip (P/N: 760435) HT DNA5K/RNA/CZE Chip 1 Safety Warning & Precautions ! WARNING ! For Research Use Only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals. CAUTION We recommend that this product and components be handled only by those who have been trained in laboratory techniques and that it is used in accordance with the principles of good laboratory practice. As all chemicals should be considered as potentially hazardous, it is advisable when handling chemical reagents to wear suitable protective clothing, such as laboratory overalls, safety glasses, and gloves. Care should be taken to avoid contact with skin or eyes. In case of contact with skin or eyes, wash immediately with water. ! WARNING ! Dye concentrate contains DMAC. Avoid inhalation and contact with skin and eyes. Storage: When not in use, store chips and buffers refrigerated at 4 °C; store labeling reagents at -20 °C. Do not leave chips, buffers and reagents at room temperature overnight. _________________________________________________________________________________________ Caliper - a PerkinElmer Company Page 3 of 18 PN: CLS135474 Rev. 02 68 Elm Street Hopkinton, MA 01748-1668 1-877-LABCHIP (1-877-522-2447)
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HT Protein Charge Variant Kit User Guide - PerkinElmer

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