Book with abstracts from the COST Action 0905 meeting in ... - UMB
Book with abstracts from the COST Action 0905 meeting in ... - UMB
Book with abstracts from the COST Action 0905 meeting in ... - UMB
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POSSIBLE NICOTIANAMINE INVOLVEMENT IN CADMIUM<br />
METABOLISM IN TOMATO PLANTS<br />
K.Tracz 1 , S. Clemens 2 and D.M. Antosiewicz 1<br />
1 University of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology and<br />
Biotechnology, Miecznikowa 1, 02-096 Warsaw, Poland<br />
2 University of Bayreuth, Department of Plant Physiology, Universitätsstrasse 30, 95449<br />
Bayreuth, Germany<br />
e-mails: ktracz@biol.uw.edu.pl; dma@biol.uw.edu.pl<br />
Keywords: cadmium, nicotianam<strong>in</strong>e, tomato<br />
Due to cadmium toxicity <strong>the</strong> research <strong>in</strong>to reduc<strong>in</strong>g its amount <strong>in</strong> edible plant parts<br />
is necessary. We have generated tomato plants express<strong>in</strong>g AhNAS2p::AhNAS2 <strong>from</strong><br />
A.halleri, encod<strong>in</strong>g nicotianam<strong>in</strong>e synthase (NAS), an enzyme <strong>in</strong>volved <strong>in</strong> <strong>the</strong> biosyn<strong>the</strong>sis<br />
of nicotianam<strong>in</strong>e (NA), chelator of Fe 2+ , Fe 3+ , Ni 2+ , Zn 2+ , Cu 2+ . Until now, <strong>the</strong>re was no<br />
<strong>in</strong>dication of NA <strong>in</strong>volvement <strong>in</strong> <strong>the</strong> metabolism of Cd.<br />
Transgenic and wild-type plants were exposed to Cd (0,25µM) and different Fe<br />
regimes: 0,1µM, 10µM, 100µM. Plant tolerance to growth conditions and accumulation of<br />
Fe, Zn and Cd were evaluated.<br />
The difference <strong>in</strong> Fe-deficiency tolerance between plants not detected dur<strong>in</strong>g <strong>the</strong><br />
first 7-9-days of exposure (all l<strong>in</strong>es had leaves <strong>with</strong> yellow areas) became apparent on <strong>the</strong><br />
day 8-12 th . Leaves of transgenics turned <strong>from</strong> yellow to green and Fe level was higher<br />
than <strong>in</strong> WTs. Prelim<strong>in</strong>ary results <strong>in</strong>dicate also that transgenics exposed to<br />
0.1µMFe/0,25µMCd had lower Cd concentration. In total, <strong>the</strong>y accumulated less Fe but<br />
be<strong>in</strong>g more efficient <strong>in</strong> translocation Fe to leaves, its level rema<strong>in</strong>ed as <strong>in</strong> WT.<br />
Switch <strong>in</strong> <strong>the</strong> phenotype of transformants after 10-day of growth at low Fe <strong>in</strong>dicates<br />
developmental regulation of AhNAS2p::AhNAS2 <strong>in</strong> tomato and contribution of NA to<br />
<strong>in</strong>crease <strong>in</strong> Fe-deficiency tolerance. Decreased Cd concentrations <strong>in</strong> leaves of<br />
transformants <strong>in</strong>dicate possible <strong>in</strong>volvement of NA <strong>in</strong> Cd-b<strong>in</strong>d<strong>in</strong>g and regulation of Cd<br />
root-to-shoot translocation.<br />
Acknowledgements: This work was supported by FP6 EU PHIME project (FOOD-CT-2006-<br />
016253), and <strong>COST</strong> FA <strong>0905</strong>.